MGST1

Summary

MGST1 (microsomal glutathione S-transferase 1, HGNC:7061) is a protein-coding gene on chromosome 12p12.3, encoding Microsomal glutathione S-transferase 1 (P10620). Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.

Source: NCBI Gene 4257 — RefSeq curated summary.

At a glance

• GWAS associations: 10
• Clinical variants (ClinVar): 43 total
• Druggable target: yes
• MANE Select transcript: NM_020300

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7061
Approved symbol	MGST1
Name	microsomal glutathione S-transferase 1
Location	12p12.3
Locus type	gene with protein product
Status	Approved
Aliases	MGST-I, PMAN
Ensembl gene	ENSG00000008394
Ensembl biotype	protein_coding
OMIM	138330
Entrez	4257

Gene structure

Transcript identifiers

Ensembl transcripts: 50 — 41 protein_coding, 6 protein_coding_CDS_not_defined, 2 retained_intron, 1 TEC

ENST00000010404, ENST00000359720, ENST00000396207, ENST00000396209, ENST00000396210, ENST00000534835, ENST00000535309, ENST00000535624, ENST00000536371, ENST00000537878, ENST00000538857, ENST00000538885, ENST00000539036, ENST00000539708, ENST00000540056, ENST00000540126, ENST00000542256, ENST00000543076, ENST00000624056, ENST00000902095, ENST00000902096, ENST00000902097, ENST00000902098, ENST00000902099, ENST00000902100, ENST00000902101, ENST00000902102, ENST00000902103, ENST00000902104, ENST00000902105, ENST00000902106, ENST00000902107, ENST00000902108, ENST00000902109, ENST00000902110, ENST00000902111, ENST00000902112, ENST00000902113, ENST00000902114, ENST00000902115, ENST00000902116, ENST00000921235, ENST00000921236, ENST00000921237, ENST00000921238, ENST00000921239, ENST00000921240, ENST00000921241, ENST00000960433, ENST00000960434

RefSeq mRNA: 24 — MANE Select: NM_020300 NM_001260511, NM_001260512, NM_001267598, NM_001414355, NM_001414356, NM_001414357, NM_001414358, NM_001414359, NM_001414361, NM_001414362, NM_001414363, NM_001414365, NM_001414367, NM_001414368, NM_001414369, NM_001414370, NM_001414371, NM_001414372, NM_001414373, NM_001414374, NM_020300, NM_145764, NM_145791, NM_145792

CCDS: CCDS58209, CCDS8677

Canonical transcript exons

ENST00000396210 — 4 exons

Exon	Start	End
ENSE00001524244	16363795	16364410
ENSE00001524245	16347645	16347710
ENSE00003528377	16354231	16354378
ENSE00003669648	16357605	16357699

Expression profiles

Bgee: expression breadth ubiquitous, 258 present calls, max score 99.86.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 302.1066 / max 5232.6914, expressed in 1686 samples.

FANTOM5 promoters (13 alternative TSS)

Promoter ID	TPM avg	Samples expressed
124524	247.8202	1682
124525	35.5051	1622
124522	7.9324	1098
124523	7.5410	1330
124521	0.8104	287
124529	0.5085	230
124531	0.4465	216
124527	0.3618	155
124530	0.3036	134
124528	0.2852	121

Top tissues by expression

259 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right adrenal gland	UBERON:0001233	99.86	gold quality
right adrenal gland cortex	UBERON:0035827	99.86	gold quality
left adrenal gland	UBERON:0001234	99.83	gold quality
adrenal cortex	UBERON:0001235	99.81	gold quality
left adrenal gland cortex	UBERON:0035825	99.80	gold quality
right lobe of liver	UBERON:0001114	99.75	gold quality
ileal mucosa	UBERON:0000331	99.74	gold quality
kidney epithelium	UBERON:0004819	99.72	gold quality
adipose tissue	UBERON:0001013	99.65	gold quality
adrenal gland	UBERON:0002369	99.65	gold quality
adipose tissue of abdominal region	UBERON:0007808	99.61	gold quality
liver	UBERON:0002107	99.60	gold quality
omental fat pad	UBERON:0010414	99.60	gold quality
peritoneum	UBERON:0002358	99.58	gold quality
subcutaneous adipose tissue	UBERON:0002190	99.57	gold quality
pericardium	UBERON:0002407	99.46	gold quality
upper leg skin	UBERON:0004262	99.45	gold quality
right lobe of thyroid gland	UBERON:0001119	99.41	gold quality
left lobe of thyroid gland	UBERON:0001120	99.36	gold quality
adrenal tissue	UBERON:0018303	99.35	gold quality
upper arm skin	UBERON:0004263	99.32	gold quality
nasal cavity epithelium	UBERON:0005384	99.30	gold quality
mucosa of transverse colon	UBERON:0004991	99.27	gold quality
thyroid gland	UBERON:0002046	99.22	gold quality
decidua	UBERON:0002450	99.20	gold quality
stromal cell of endometrium	CL:0002255	99.19	gold quality
colonic mucosa	UBERON:0000317	99.15	gold quality
thoracic mammary gland	UBERON:0005200	99.13	gold quality
mucosa of sigmoid colon	UBERON:0004993	99.09	gold quality
mammary gland	UBERON:0001911	99.07	gold quality

Single-cell (SCXA)

Detected in 36 experiment(s), a significant marker in 35.

Experiment	Marker?	Max mean expression
E-MTAB-10553	yes	3501.15
E-HCAD-9	yes	3460.61
E-MTAB-8495	yes	3414.31
E-MTAB-6308	yes	3213.18
E-HCAD-15	yes	1663.43
E-MTAB-6701	yes	1515.55
E-HCAD-1	yes	1165.50
E-HCAD-24	yes	1163.44
E-CURD-126	yes	1036.50
E-GEOD-84465	yes	856.09
E-MTAB-5061	yes	850.67
E-MTAB-8884	yes	688.86
E-CURD-46	yes	580.08
E-GEOD-81547	yes	530.89
E-MTAB-11121	yes	498.04

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): MYC, NFE2L2, SP1

miRNA regulators (miRDB)

24 targeting MGST1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-8087	99.90	69.55	1351
HSA-MIR-4495	99.82	72.08	3080
HSA-MIR-7161-5P	99.68	68.92	1592
HSA-MIR-548U	99.65	67.78	1463
HSA-MIR-10393-5P	99.65	68.01	1368
HSA-MIR-513A-3P	99.39	70.63	3620
HSA-MIR-513C-3P	99.39	70.63	3620
HSA-MIR-664A-3P	99.22	71.08	2696
HSA-MIR-4291	99.20	68.88	2969
HSA-MIR-4528	99.18	69.77	1936
HSA-MIR-8077	99.17	66.67	862
HSA-MIR-3606-3P	99.11	69.84	3254
HSA-MIR-922	99.02	67.23	1838
HSA-MIR-3619-5P	99.00	68.87	2308
HSA-MIR-1257	98.97	68.02	1133
HSA-MIR-6074	98.89	69.64	2187
HSA-MIR-4272	98.76	68.74	1810
HSA-MIR-761	98.71	68.07	2051
HSA-MIR-214-3P	98.71	68.12	2128
HSA-MIR-640	98.44	66.93	644
HSA-MIR-10397-3P	97.78	65.70	601
HSA-MIR-376A-5P	97.70	65.61	863
HSA-MIR-6818-5P	97.50	67.10	1167

Literature-anchored findings (GeneRIF, showing 16)

• As an addition to the high-density SNP (single-nucleotide polymorphism) maps of 12 glutathione S-transferase and related genes reported earlier, a SNP map of the microsomal glutathione S-transferase 1 (MGST1) gene is provided. (PMID:11587073)
• The results indicate that Sp1 is the protein mediating the basal transcription of MGST1 (PMID:12818425)
• Genes encoding KU70, MGST1 and BIK show age-related mRNA expression levels in hematopoietic stem cells. (PMID:17714764)
• MGST1 protects cells (and mitochondria) by both conjugation and glutathione peroxidase functions. A new protective mechanism against cisplatin is also indicated (PMID:20727966)
• The results suggest that MGST1 gene polymorphisms as one of environment gene may contribute to colorectal cancer risk in younger ages. (PMID:23314968)
• Differential expression of SOD2, ALDH1A1, and MGST1 genes in the anterior lens capsules of patients with pseudoexfoliation syndrome suggest that diseased tissue appears to respond to the previously reported oxidative stress. (PMID:23805041)
• possibly marked variations in NB mitochondrial homeostasis, they also imply that the results of these earlier studies using NB cells are not transferable to other tumor and cell types that express MGST1 at high concentrations. (PMID:24486338)
• This study provides the most comprehensive evaluation of inflammation-related germline variation in relation to risk of BE/OA and suggests that variants in MGST1 influence disease susceptibility. (PMID:27486097)
• The MSGT1 displays a preexisting dynamic equilibrium between high- and low-activity forms. (PMID:28558199)
• High MGST1 expression is associated with Bendamustine-resistance in Mantle Cell Lymphoma. (PMID:29695404)
• Significant association for germline variations only in the COX pathway (specifically in the antioxidant microsomal glutathione S-transferase 1 [MGST1] gene), with risk for Barrett’s esophagus and the combined outcome of Barrett’s esophagus and esophageal adenocarcinoma was found. (PMID:29713984)
• MGST1 plays an important role in lung adenocarcinoma (LUAD)tumorigenesis and might serve as a potential prognostic factor and therapeutic target in LUAD. (PMID:31707341)
• MGST1 is a redox-sensitive repressor of ferroptosis in pancreatic cancer cells. (PMID:33539732)
• MiR-379-5p targets microsomal glutathione transferase 1 (MGST1) to regulate human glioma in cell proliferation, migration and invasion and epithelial-mesenchymal transition (EMT). (PMID:34171541)
• Microsomal glutathione S-transferase 1 targets the autophagy signaling pathway to suppress ferroptosis in gastric carcinoma cells. (PMID:37161854)
• A role for microsomal glutathione transferase 1 in melanin biosynthesis and melanoma progression. (PMID:37321450)

Cross-species orthologs

7 orthologs

Organism	Symbol	Gene ID
danio_rerio	mgst1.2	ENSDARG00000022165
danio_rerio	mgst1.1	ENSDARG00000032618
mus_musculus	Mgst1	ENSMUSG00000008540
rattus_norvegicus	Mgst1	ENSRNOG00000007743
drosophila_melanogaster	Mgstl	FBGN0025814
drosophila_melanogaster	CG33177	FBGN0053177
drosophila_melanogaster	CG33178	FBGN0053178

Paralogs (1): PTGES (ENSG00000148344)

Protein

Protein identifiers

Microsomal glutathione S-transferase 1 — P10620 (reviewed: P10620)

Alternative names: Microsomal GST-I

All UniProt accessions (6): P10620, F5GX73, F5H613, F5H6X2, F5H760, F5H7F6

UniProt curated annotations — full annotation on UniProt →

Function. Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Subunit / interactions. Homotrimer; The trimer binds only one molecule of glutathione.

Subcellular location. Endoplasmic reticulum membrane. Mitochondrion outer membrane.

Tissue specificity. Highly expressed in liver.

Similarity. Belongs to the MAPEG family.

Isoforms (2)

UniProt ID	Names	Canonical?
P10620-1	1	yes
P10620-2	2

RefSeq proteins (24): NP_001247440, NP_001247441, NP_001254527, NP_001401284, NP_001401285, NP_001401286, NP_001401287, NP_001401288, NP_001401290, NP_001401291, NP_001401292, NP_001401294, NP_001401296, NP_001401297, NP_001401298, NP_001401299, NP_001401300, NP_001401301, NP_001401302, NP_001401303, NP_064696, NP_665707, NP_665734, NP_665735 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001129	Membr-assoc_MAPEG	Family
IPR023352	MAPEG-like_dom_sf	Homologous_superfamily
IPR040162	MGST1-like	Family

Pfam: PF01124

Catalyzed reactions (Rhea), 1 shown:

• RX + glutathione = an S-substituted glutathione + a halide anion + H(+) (RHEA:16437)

UniProt features (20 total): binding site 6, topological domain 5, transmembrane region 4, modified residue 3, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P10620-F1	78.65	0.07

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (6): 38; 73; 74; 76; 81; 121

Post-translational modifications (3): 42, 55, 60

Function

Pathways and Gene Ontology

Reactome pathways

8 pathways

ID	Pathway
R-HSA-156590	Glutathione conjugation
R-HSA-5423646	Aflatoxin activation and detoxification
R-HSA-6798695	Neutrophil degranulation
R-HSA-1430728	Metabolism
R-HSA-156580	Phase II - Conjugation of compounds
R-HSA-168249	Innate Immune System
R-HSA-168256	Immune System
R-HSA-211859	Biological oxidations

MSigDB gene sets: 282 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_UP, REACTOME_BIOLOGICAL_OXIDATIONS, REACTOME_INNATE_IMMUNE_SYSTEM, GOBP_CELLULAR_RESPONSE_TO_LIPID, GOCC_VACUOLAR_MEMBRANE, GOCC_SECRETORY_GRANULE, MODULE_151, GOBP_MODIFIED_AMINO_ACID_TRANSPORT, GOBP_CELLULAR_RESPONSE_TO_OXYGEN_CONTAINING_COMPOUND, GOMF_GLUTATHIONE_TRANSFERASE_ACTIVITY, GOLDRATH_ANTIGEN_RESPONSE, HERNANDEZ_MITOTIC_ARREST_BY_DOCETAXEL_2_UP, WEI_MYCN_TARGETS_WITH_E_BOX, SCHUHMACHER_MYC_TARGETS_UP, GOBP_CELLULAR_RESPONSE_TO_TOXIC_SUBSTANCE

GO Biological Process (3): glutathione transport (GO:0034635), cellular response to lipid hydroperoxide (GO:0071449), cellular oxidant detoxification (GO:0098869)

GO Molecular Function (4): glutathione transferase activity (GO:0004364), glutathione peroxidase activity (GO:0004602), protein binding (GO:0005515), transferase activity (GO:0016740)

GO Cellular Component (8): mitochondrion (GO:0005739), mitochondrial outer membrane (GO:0005741), peroxisomal membrane (GO:0005778), endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789), plasma membrane (GO:0005886), membrane (GO:0016020), azurophil granule membrane (GO:0035577)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
Biological oxidations	2
Phase II - Conjugation of compounds	1
Innate Immune System	1
Immune System	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cytoplasm	2
intracellular membrane-bounded organelle	2
tripeptide transport	1
modified amino acid transport	1
sulfur compound transport	1
response to lipid hydroperoxide	1
cellular response to lipid	1
cellular response to hydroperoxide	1
cellular detoxification	1
transferase activity, transferring alkyl or aryl (other than methyl) groups	1
peroxidase activity	1
binding	1
catalytic activity	1
mitochondrial membrane	1
organelle outer membrane	1
peroxisome	1
microbody membrane	1
endomembrane system	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
membrane	1
cell periphery	1
cellular anatomical structure	1
lysosomal membrane	1
secretory granule membrane	1
azurophil granule	1

Protein interactions and networks

STRING

1220 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MGST1	MGST2	Q99735	968
MGST1	MGST3	O14880	948
MGST1	LTC4S	Q16873	916
MGST1	ALOX5AP	P20292	894
MGST1	HPGDS	O60760	759
MGST1	GSTA3	Q16772	663
MGST1	SLCO6A1	Q86UG4	646
MGST1	GSTO1	P78417	589
MGST1	GSTK1	Q9Y2Q3	564
MGST1	GSTM2	P28161	532
MGST1	GSTP1	P09211	531
MGST1	ALOX5	P09917	515
MGST1	GSTA1	P08263	504
MGST1	GSTT2B	P0CG30	497
MGST1	PPIF	P30405	494

IntAct

79 interactions, top by confidence:

A	B	Type	Score
RAF1	CALU	psi-mi:“MI:0914”(association)	0.640
VIP	MGST1	psi-mi:“MI:0915”(physical association)	0.560
FZD7	MGST1	psi-mi:“MI:0915”(physical association)	0.560
MGST1	SIGLEC12	psi-mi:“MI:0915”(physical association)	0.560
EGF	MGST1	psi-mi:“MI:0915”(physical association)	0.560
TNFRSF10C	MGST1	psi-mi:“MI:0915”(physical association)	0.560
MGST1	FXYD3	psi-mi:“MI:0915”(physical association)	0.560
ARMC6	SLC27A2	psi-mi:“MI:0914”(association)	0.530
ANKH	FAM234B	psi-mi:“MI:0914”(association)	0.530
ERBB2	NDUFA4	psi-mi:“MI:0914”(association)	0.530
NELFB	MGST1	psi-mi:“MI:0915”(physical association)	0.520
RAB11A	LANCL1	psi-mi:“MI:2364”(proximity)	0.420
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350
	HAX1	psi-mi:“MI:0914”(association)	0.350
Prdm16	ESYT2	psi-mi:“MI:0914”(association)	0.350
Mecom	ESYT2	psi-mi:“MI:0914”(association)	0.350
LRRK2		psi-mi:“MI:0914”(association)	0.350
NMES1	COX7A2L	psi-mi:“MI:0914”(association)	0.350
CHCHD10	ACSL4	psi-mi:“MI:0914”(association)	0.350
COQ9	ACOT7	psi-mi:“MI:0914”(association)	0.350
FAM136A	ALDH1L1	psi-mi:“MI:0914”(association)	0.350
NDUFA4	NDUFS8	psi-mi:“MI:0914”(association)	0.350

BioGRID (164): LTC4S (Two-hybrid), MGST1 (Affinity Capture-MS), MGST1 (Proximity Label-MS), MGST1 (Affinity Capture-MS), MGST1 (Affinity Capture-MS), MGST1 (Affinity Capture-RNA), MGST1 (Affinity Capture-MS), MGST1 (Affinity Capture-MS), MGST1 (Proximity Label-MS), MGST1 (Affinity Capture-MS), MGST1 (Proximity Label-MS), MGST1 (Affinity Capture-MS), MGST1 (Affinity Capture-MS), MGST1 (Proximity Label-MS), MGST1 (Co-fractionation)

ESM2 similar proteins: A0A2I1C3V3, A0SYQ0, A1L2F6, A2RST1, A4DA06, A6XA80, A8X8R3, J7FIJ6, O14684, O14880, O74507, O94511, P08011, P0DN89, P10620, P64515, P64516, P70245, P73795, P79382, Q15125, Q16873, Q17428, Q28GF8, Q296J9, Q2KJG4, Q2NKS0, Q3T100, Q41745, Q54GA9, Q57ZC7, Q5R9A6, Q60490, Q60860, Q64L89, Q6GNM0, Q6GPW4, Q6PWL6, Q8HZJ2, Q91VS7

Diamond homologs: A0SYQ0, O14684, P08011, P10620, P79382, Q64L89, Q6PWL6, Q8HZJ2, Q91VS7, Q95L14, Q9JHF3, Q9JM51

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 85 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
positive regulation of MAPK cascade	7	8.2×	9e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

43 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	24
Likely benign	6
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1778 predictions. Top by Δscore:

Variant	Effect	Δscore
12:16560408:CTTA:C	donor_loss	1.0000
12:16560409:TTAC:T	donor_loss	1.0000
12:16560410:TA:T	donor_loss	1.0000
12:16560411:A:C	donor_loss	1.0000
12:16560412:CCT:C	donor_gain	1.0000
12:16560535:GAGCC:G	acceptor_loss	1.0000
12:16560537:GC:G	acceptor_gain	1.0000
12:16560537:GCCTA:G	acceptor_loss	1.0000
12:16560538:CC:C	acceptor_gain	1.0000
12:16560538:CCT:C	acceptor_loss	1.0000
12:16560539:C:CA	acceptor_loss	1.0000
12:16560539:C:CC	acceptor_gain	1.0000
12:16560540:T:A	acceptor_loss	1.0000
12:16590509:A:AC	donor_gain	1.0000
12:16600746:T:TA	donor_gain	1.0000
12:16600864:TATAC:T	acceptor_gain	1.0000
12:16600865:ATAC:A	acceptor_gain	1.0000
12:16600866:TAC:T	acceptor_gain	1.0000
12:16600866:TACC:T	acceptor_loss	1.0000
12:16600869:C:CC	acceptor_gain	1.0000
12:16600869:CTAAA:C	acceptor_loss	1.0000
12:16354219:A:G	acceptor_gain	0.9900
12:16354228:A:AG	acceptor_gain	0.9900
12:16354229:A:G	acceptor_gain	0.9900
12:16354374:GAAAG:G	donor_gain	0.9900
12:16354376:AAG:A	donor_loss	0.9900
12:16354377:AG:A	donor_loss	0.9900
12:16354378:GG:G	donor_loss	0.9900
12:16354380:T:A	donor_loss	0.9900
12:16357600:GATA:G	acceptor_loss	0.9900

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000005682 (12:16517888 A>C,G), RS1000015119 (12:16477572 G>C,T), RS1000018649 (12:16359891 T>G), RS1000029810 (12:16359618 A>G,T), RS1000057979 (12:16571474 A>C,G), RS1000071537 (12:16469519 G>A), RS1000080146 (12:16386496 T>C), RS1000099350 (12:16488357 A>G,T), RS1000100626 (12:16426832 C>A,T), RS1000112128 (12:16398869 G>T), RS1000145160 (12:16438853 A>G), RS1000149954 (12:16553826 G>C), RS1000164506 (12:16505620 A>G,T), RS1000166261 (12:16378117 T>G), RS1000183115 (12:16516209 CAA>C)

Disease associations

OMIM: gene MIM:138330 | disease phenotypes:

GenCC curated gene-disease

Mondo (1): pulmonary disease, chronic obstructive, susceptibility to (MONDO:0100167)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

10 associations (top):

Study	Trait	p-value
GCST001525_20	Visceral fat	5.000000e-06
GCST002816_1	Colorectal cancer (aspirin and/or NSAID use interaction)	5.000000e-09
GCST009391_71	Metabolite levels	4.000000e-06
GCST009524_200	Household income (MTAG)	6.000000e-09
GCST011354_10	Bell’s palsy	8.000000e-06
GCST011461_2	Barrett’s esophagus or Esophageal adenocarcinoma	1.000000e-08
GCST011461_7	Barrett’s esophagus or Esophageal adenocarcinoma	5.000000e-09
GCST011462_2	Barrett’s esophagus or esophageal adenocarcinoma x sex interaction (2df test)	4.000000e-08
GCST011584_1	Metastatic colorectal cancer survival in treatment with chemotherapy plus biologics	1.000000e-06
GCST90026416_16	Mild age-related type 2 diabetes	8.000000e-06

EFO canonical traits (8, from GWAS)

EFO ID	Trait name
EFO:0007010	drug use measurement
EFO:0007012	NSAID use measurement
EFO:0007013	aspirin use measurement
EFO:0010353	diacylglycerol 34:2 measurement
EFO:0009695	household income
EFO:0008343	sex interaction measurement
EFO:0000714	survival time
EFO:1001480	metastatic colorectal cancer

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL1743184 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

Binding affinities (BindingDB)

2 measured of 4 human assays (4 total across all organisms); most potent 2 below. Values come from heterogeneous assays and are not directly comparable.

Ligand	Measure	Value
640/359	KI	32700 nM
CEFOPERAZONE	KI	245000 nM

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.42	Kd	3764	nM	CHEMBL5653589
5.42	ED50	3764	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 6 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148747: Binding affinity to human MGST1 incubated for 45 mins by Kinobead based pull down assay	kd	3.7638	uM

CTD chemical–gene interactions

111 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Tobacco Smoke Pollution	decreases expression, increases expression	6
Benzo(a)pyrene	increases methylation, increases expression	5
bisphenol A	affects expression, decreases expression, increases expression, increases methylation	4
sodium arsenite	increases abundance, increases expression, increases reaction, decreases expression	4
Acetaminophen	decreases expression, affects cotreatment, affects expression	4
Valproic Acid	affects cotreatment, increases expression, affects expression, decreases methylation	4
Cadmium Chloride	increases expression, decreases reaction, increases abundance, increases palmitoylation, decreases expression	4
trichostatin A	increases expression, affects cotreatment	3
Cadmium	decreases expression, increases expression, decreases reaction, increases abundance, increases palmitoylation	3
Estradiol	affects cotreatment, increases expression, decreases expression	3
Tetrachlorodibenzodioxin	decreases expression, increases expression	3
Tretinoin	decreases expression	3
lead acetate	increases expression	2
arsenite	affects binding, increases reaction, increases abundance, increases expression	2
nickel sulfate	increases expression	2
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression, increases expression	2
Arsenic	increases expression, increases abundance	2
Nickel	decreases expression	2
Sodium Selenite	decreases expression, increases expression	2
beta-Naphthoflavone	increases expression	2
bisphenol F	increases expression	1
TL8-506	affects cotreatment, increases expression	1
bismuth tripotassium dicitrate	increases expression	1
methylmercuric chloride	increases expression	1
triphenyl phosphate	affects expression	1
6-hydroxy-5-((p- sulfophenyl)azo)-2-naphthalenesulfonic acid disodium salt	affects cotreatment, increases expression	1
sodium arsenate	increases abundance, increases expression	1
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate	affects response to substance, affects expression	1
pyrogallol 1,3-dimethyl ether	affects cotreatment, increases expression	1
beta-lapachone	increases expression	1

ChEMBL screening assays

3 unique, capped per target: 2 admet, 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1743239	ADMET	Substrates for human microsomal glutathione transferase MGST1	Casarett and Doull’s Toxicology The Basic Science of Poisons, 7th edition
CHEMBL5651789	Binding	Binding affinity to human MGST1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_E1MU	HyCyte SK-HEP-1 KO-hMGST1	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.