MGST3

Summary

MGST3 (microsomal glutathione S-transferase 3, HGNC:7064) is a protein-coding gene on chromosome 1q24.1, encoding Glutathione S-transferase 3, mitochondrial (O14880). Displays both glutathione S-transferase and glutathione peroxidase activities toward oxyeicosanoids, as part of cellular detoxification as well as synthesis of bioactive metabolites.

This gene encodes a member of the MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) protein family. Members of this family are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes an enzyme which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. This enzyme also demonstrates glutathione-dependent peroxidase activity towards lipid hydroperoxides.

Source: NCBI Gene 4259 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 44 total
• Druggable target: yes
• MANE Select transcript: NM_004528

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:7064
Approved symbol	MGST3
Name	microsomal glutathione S-transferase 3
Location	1q24.1
Locus type	gene with protein product
Status	Approved
Aliases	GST-III
Ensembl gene	ENSG00000143198
Ensembl biotype	protein_coding
OMIM	604564
Entrez	4259

Gene structure

Transcript identifiers

Ensembl transcripts: 43 — 35 protein_coding, 5 retained_intron, 3 protein_coding_CDS_not_defined

ENST00000367883, ENST00000367884, ENST00000367885, ENST00000367888, ENST00000367889, ENST00000460632, ENST00000461308, ENST00000461759, ENST00000477450, ENST00000488688, ENST00000494074, ENST00000495447, ENST00000609263, ENST00000627653, ENST00000855187, ENST00000855188, ENST00000855189, ENST00000855190, ENST00000855191, ENST00000855192, ENST00000855193, ENST00000855194, ENST00000855195, ENST00000855196, ENST00000855197, ENST00000855198, ENST00000922014, ENST00000922015, ENST00000922016, ENST00000922017, ENST00000922018, ENST00000922019, ENST00000922020, ENST00000922021, ENST00000922022, ENST00000922023, ENST00000969013, ENST00000969014, ENST00000969015, ENST00000969016, ENST00000969017, ENST00000969018, ENST00000969019

RefSeq mRNA: 1 — MANE Select: NM_004528 NM_004528

CCDS: CCDS1249

Canonical transcript exons

ENST00000367889 — 6 exons

Exon	Start	End
ENSE00001931710	165631234	165631293
ENSE00003545335	165651978	165652035
ENSE00003598372	165649841	165649964
ENSE00003608858	165654279	165654351
ENSE00003680235	165651014	165651087
ENSE00003848969	165655368	165656136

Expression profiles

Bgee: expression breadth ubiquitous, 293 present calls, max score 99.74.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 135.1088 / max 1659.4271, expressed in 1822 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
6392	133.7429	1822
6391	1.3659	755

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
corpus epididymis	UBERON:0004359	99.74	gold quality
jejunal mucosa	UBERON:0000399	99.67	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	99.59	gold quality
pons	UBERON:0000988	99.58	gold quality
heart right ventricle	UBERON:0002080	99.54	gold quality
diaphragm	UBERON:0001103	99.51	gold quality
adult organism	UBERON:0007023	99.50	gold quality
apex of heart	UBERON:0002098	99.46	gold quality
duodenum	UBERON:0002114	99.45	gold quality
biceps brachii	UBERON:0001507	99.43	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	99.43	gold quality
mucosa of sigmoid colon	UBERON:0004993	99.38	gold quality
lateral nuclear group of thalamus	UBERON:0002736	99.32	gold quality
cardiac ventricle	UBERON:0002082	99.30	gold quality
jejunum	UBERON:0002115	99.30	gold quality
mucosa of transverse colon	UBERON:0004991	99.30	gold quality
heart left ventricle	UBERON:0002084	99.29	gold quality
right atrium auricular region	UBERON:0006631	99.27	gold quality
colonic mucosa	UBERON:0000317	99.26	gold quality
prefrontal cortex	UBERON:0000451	99.26	gold quality
hindlimb stylopod muscle	UBERON:0004252	99.22	gold quality
triceps brachii	UBERON:0001509	99.18	gold quality
trabecular bone tissue	UBERON:0002483	99.17	gold quality
Brodmann (1909) area 46	UBERON:0006483	99.16	gold quality
amygdala	UBERON:0001876	99.15	gold quality
substantia nigra pars compacta	UBERON:0001965	99.13	gold quality
body of tongue	UBERON:0011876	99.11	gold quality
heart	UBERON:0000948	99.07	gold quality
synovial joint	UBERON:0002217	99.07	gold quality
descending thoracic aorta	UBERON:0002345	99.07	gold quality

Single-cell (SCXA)

Detected in 17 experiment(s), a significant marker in 13.

Experiment	Marker?	Max mean expression
E-MTAB-10042	yes	1171.95
E-MTAB-6701	yes	133.90
E-CURD-122	yes	67.71
E-MTAB-10287	yes	45.04
E-GEOD-134144	yes	44.45
E-CURD-112	yes	43.70
E-HCAD-10	yes	38.84
E-HCAD-1	yes	33.00
E-MTAB-8410	yes	24.65
E-HCAD-11	yes	19.18
E-CURD-46	yes	11.10
E-HCAD-9	yes	7.98
E-CURD-98	no	1293.87
E-MTAB-8559	no	885.39
E-GEOD-125970	no	6.24

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

32 targeting MGST3, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4481	100.00	66.42	1669
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-4745-5P	99.98	65.95	1028
HSA-MIR-548M	99.70	68.87	1749
HSA-MIR-5093	99.67	69.26	2291
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-5700	99.64	69.88	2280
HSA-MIR-875-3P	99.63	69.47	2548
HSA-MIR-3975	99.62	65.97	697
HSA-MIR-3942-3P	99.57	69.03	2854
HSA-MIR-4437	99.52	65.29	1266
HSA-MIR-19A-5P	99.36	66.93	1675
HSA-MIR-3692-5P	99.29	67.04	1421
HSA-MIR-485-5P	99.10	64.78	1889
HSA-MIR-6884-5P	99.10	64.50	1987
HSA-MIR-5006-5P	98.79	66.92	1246
HSA-MIR-3188	98.58	65.60	878
HSA-MIR-3944-5P	98.50	67.55	997
HSA-MIR-499B-5P	98.35	68.39	988
HSA-MIR-4433A-3P	97.75	62.82	1435
HSA-MIR-6509-5P	97.39	68.27	969
HSA-MIR-2467-5P	97.36	67.71	991
HSA-MIR-645	97.28	66.30	486
HSA-MIR-3137	97.26	66.78	761
HSA-MIR-5702	96.68	68.21	958
HSA-MIR-191-5P	95.88	67.82	171
HSA-MIR-1238-5P	94.82	67.52	493

Literature-anchored findings (GeneRIF, showing 1)

• Variants in MGST3 are associated with differences inn hippocampus size in both human brain data sets (ENIGMA MRI) and in the BXD family of mice. MGST3 is also statistically associated to genes linked to neurodegenerative disorders, including Parkinson’s, Huntington’s, and Alzheimer’s diseases. (PMID:25280473)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	mgst3a	ENSDARG00000102744
mus_musculus	Mgst3	ENSMUSG00000026688
rattus_norvegicus	Utp3	ENSRNOG00000003599
rattus_norvegicus	Mgst3	ENSRNOG00000004245

Paralogs (3): MGST2 (ENSG00000085871), ALOX5AP (ENSG00000132965), LTC4S (ENSG00000213316)

Protein

Protein identifiers

Glutathione S-transferase 3, mitochondrial — O14880 (reviewed: O14880)

Alternative names: Glutathione peroxidase MGST3, LTC4 synthase MGST3

All UniProt accessions (3): O14880, Q5VV87, Q5VV89

UniProt curated annotations — full annotation on UniProt →

Function. Displays both glutathione S-transferase and glutathione peroxidase activities toward oxyeicosanoids, as part of cellular detoxification as well as synthesis of bioactive metabolites. Catalyzes conjugate addition of reduced glutathione to the alpha, beta-unsaturated C=C carbonyl group of eisosanoids such as leukotriene A4 and 15-deoxy-Delta12,14-prostaglandin J2 to form GSH adducts relevant to the inflammatory response. Catalyzes glutathione-dependent reduction of eicosanoid peroxides to yield the corresponding eicosanoid hydroxides.

Subcellular location. Mitochondrion outer membrane.

Tissue specificity. Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes. Expressed in mast cells.

Pathway. Lipid metabolism; leukotriene C4 biosynthesis. Lipid metabolism; arachidonate metabolism.

Similarity. Belongs to the MAPEG family.

RefSeq proteins (1): NP_004519* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001129	Membr-assoc_MAPEG	Family
IPR023352	MAPEG-like_dom_sf	Homologous_superfamily
IPR050997	MAPEG	Family

Pfam: PF01124

Catalyzed reactions (Rhea), 3 shown:

• leukotriene C4 = leukotriene A4 + glutathione (RHEA:17617)
• (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O (RHEA:48620)
• 15-deoxy-Delta(12,14)-prostaglandin J2 + glutathione = 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathione (RHEA:75963)

UniProt features (14 total): topological domain 4, transmembrane region 3, sequence variant 2, mutagenesis site 2, lipid moiety-binding region 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O14880-F1	92.78	0.88

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 150, 151

Mutagenesis-validated functional residues (2):

Position	Phenotype
150	abolishes s-acylation; when associated with s-151.
151	abolishes s-acylation; when associated with s-150.

Function

Pathways and Gene Ontology

Reactome pathways

5 pathways

ID	Pathway
R-HSA-156590	Glutathione conjugation
R-HSA-5423646	Aflatoxin activation and detoxification
R-HSA-1430728	Metabolism
R-HSA-156580	Phase II - Conjugation of compounds
R-HSA-211859	Biological oxidations

MSigDB gene sets: 264 (showing top): MODULE_93, REACTOME_BIOLOGICAL_OXIDATIONS, STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, IVANOVA_HEMATOPOIESIS_MATURE_CELL, GOBP_LONG_CHAIN_FATTY_ACID_METABOLIC_PROCESS, TGACCTY_ERR1_Q2, GOBP_LEUKOTRIENE_BIOSYNTHETIC_PROCESS, GOBP_MONOCARBOXYLIC_ACID_METABOLIC_PROCESS, GOBP_ORGANIC_ACID_BIOSYNTHETIC_PROCESS, GOMF_GLUTATHIONE_TRANSFERASE_ACTIVITY, SENESE_HDAC1_AND_HDAC2_TARGETS_DN, GOBP_SMALL_MOLECULE_BIOSYNTHETIC_PROCESS, MODULE_75, GOBP_CELLULAR_RESPONSE_TO_TOXIC_SUBSTANCE

GO Biological Process (6): lipid metabolic process (GO:0006629), prostanoid metabolic process (GO:0006692), arachidonate metabolic process (GO:0019369), leukotriene biosynthetic process (GO:0019370), leukotriene metabolic process (GO:0006691), cellular oxidant detoxification (GO:0098869)

GO Molecular Function (8): glutathione transferase activity (GO:0004364), leukotriene-C4 synthase activity (GO:0004464), glutathione peroxidase activity (GO:0004602), identical protein binding (GO:0042802), protein binding (GO:0005515), oxidoreductase activity (GO:0016491), transferase activity (GO:0016740), lyase activity (GO:0016829)

GO Cellular Component (6): nuclear envelope (GO:0005635), mitochondrion (GO:0005739), mitochondrial outer membrane (GO:0005741), endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
Biological oxidations	2
Phase II - Conjugation of compounds	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
icosanoid metabolic process	3
catalytic activity	3
unsaturated fatty acid metabolic process	2
endomembrane system	2
cytoplasm	2
intracellular membrane-bounded organelle	2
primary metabolic process	1
long-chain fatty acid metabolic process	1
olefinic compound metabolic process	1
leukotriene metabolic process	1
icosanoid biosynthetic process	1
cellular detoxification	1
transferase activity, transferring alkyl or aryl (other than methyl) groups	1
carbon-sulfur lyase activity	1
peroxidase activity	1
protein binding	1
binding	1
nucleus	1
organelle envelope	1
mitochondrial membrane	1
organelle outer membrane	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
cellular anatomical structure	1

Protein interactions and networks

STRING

1506 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MGST3	MGST1	P10620	948
MGST3	LTC4S	Q16873	896
MGST3	ALOX5AP	P20292	848
MGST3	PTGES	O14684	828
MGST3	GSTM4	Q03013	619
MGST3	HPGDS	O60760	613
MGST3	GSTM2	P28161	604
MGST3	OPLAH	O14841	567
MGST3	GSTO1	P78417	529
MGST3	GSTZ1	O43708	515
MGST3	MGST2	Q99735	509
MGST3	GSTP1	P09211	492
MGST3	LRRC52	Q8N7C0	490
MGST3	POGK	Q9P215	475
MGST3	GSTA2	P09210	456
MGST3	CADM3	Q8N126	456

IntAct

340 interactions, top by confidence:

A	B	Type	Score
MGST3	TFAM	psi-mi:“MI:0915”(physical association)	0.660
CCNC	MGST3	psi-mi:“MI:0915”(physical association)	0.560
PLN	MGST3	psi-mi:“MI:0915”(physical association)	0.560
SLC7A1	MGST3	psi-mi:“MI:0915”(physical association)	0.560
MGST3	AQP3	psi-mi:“MI:0915”(physical association)	0.560
NKG7	MGST3	psi-mi:“MI:0915”(physical association)	0.560
BET1	MGST3	psi-mi:“MI:0915”(physical association)	0.560
PLP1	MGST3	psi-mi:“MI:0915”(physical association)	0.560
MGST3	MTIF3	psi-mi:“MI:0915”(physical association)	0.560
LRP10	MGST3	psi-mi:“MI:0915”(physical association)	0.560
TSPAN2	MGST3	psi-mi:“MI:0915”(physical association)	0.560
GRM2	MGST3	psi-mi:“MI:0915”(physical association)	0.560
MGST3	CREB3L1	psi-mi:“MI:0915”(physical association)	0.560
MGST3	PLP1	psi-mi:“MI:0915”(physical association)	0.560
MGST3	NRM	psi-mi:“MI:0915”(physical association)	0.560
MGST3	PAGE1	psi-mi:“MI:0915”(physical association)	0.560
MGST3	CACNG1	psi-mi:“MI:0915”(physical association)	0.560
MGST3	SCN3B	psi-mi:“MI:0915”(physical association)	0.560
MGST3	VAMP3	psi-mi:“MI:0915”(physical association)	0.560
MGST3	AQP10	psi-mi:“MI:0915”(physical association)	0.560
MGST3	TMX2	psi-mi:“MI:0915”(physical association)	0.560
MGST3	AGTRAP	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (316): MGST3 (Two-hybrid), MGST3 (Affinity Capture-RNA), MGST3 (Proximity Label-MS), MGST3 (Proximity Label-MS), MGST3 (Affinity Capture-MS), MGST3 (Affinity Capture-MS), MGST3 (Affinity Capture-MS), MGST3 (Affinity Capture-MS), MGST3 (Affinity Capture-MS), HRAS (Affinity Capture-MS), S100A13 (Affinity Capture-MS), NMT1 (Affinity Capture-MS), GLMN (Affinity Capture-MS), NMT2 (Affinity Capture-MS), GAPDHS (Affinity Capture-MS)

ESM2 similar proteins: A0A2I1C3V3, A0SYQ0, A1L2F6, A2RST1, A4DA06, A6XA80, A8X8R3, J7FIJ6, O14684, O14880, O74507, O94511, P08011, P0DN89, P10620, P64515, P64516, P70245, P73795, P79382, Q15125, Q16873, Q17428, Q28GF8, Q296J9, Q2KJG4, Q2NKS0, Q3T100, Q41745, Q54GA9, Q57ZC7, Q5R9A6, Q60490, Q60860, Q64L89, Q6GNM0, Q6GPW4, Q6PWL6, Q8HZJ2, Q91VS7

Diamond homologs: A2RST1, A6XA80, O14880, P20291, P20292, P30353, P30354, P30355, P30356, P30357, P30358, P73795, Q148F2, Q16873, Q2KJG4, Q2NKS0, Q2PG08, Q3T100, Q60860, Q925U2, Q99735, Q9CPU4, Q54GA9

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

44 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	19
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

969 predictions. Top by Δscore:

Variant	Effect	Δscore
1:165649839:A:AG	acceptor_gain	1.0000
1:165649840:G:GA	acceptor_gain	1.0000
1:165649840:GAC:G	acceptor_gain	1.0000
1:165649963:AGGTA:A	donor_loss	1.0000
1:165649964:GGTA:G	donor_loss	1.0000
1:165649965:GT:G	donor_loss	1.0000
1:165649966:T:A	donor_loss	1.0000
1:165651012:A:AG	acceptor_gain	1.0000
1:165651013:G:GG	acceptor_gain	1.0000
1:165649835:CCACA:C	acceptor_loss	0.9900
1:165649836:CACA:C	acceptor_loss	0.9900
1:165649837:ACAG:A	acceptor_loss	0.9900
1:165649838:CAGAC:C	acceptor_loss	0.9900
1:165649839:A:C	acceptor_loss	0.9900
1:165649840:G:A	acceptor_loss	0.9900
1:165649961:GGAG:G	donor_gain	0.9900
1:165649962:GAG:G	donor_gain	0.9900
1:165649962:GAGG:G	donor_gain	0.9900
1:165649965:G:GG	donor_gain	0.9900
1:165651013:GT:G	acceptor_gain	0.9900
1:165651088:G:GG	donor_gain	0.9900
1:165653847:C:G	donor_gain	0.9900
1:165654277:A:AG	acceptor_gain	0.9900
1:165654278:G:GG	acceptor_gain	0.9900
1:165654278:GC:G	acceptor_gain	0.9900
1:165655366:A:AG	acceptor_gain	0.9900
1:165655367:G:GA	acceptor_gain	0.9900
1:165649839:AGAC:A	acceptor_gain	0.9800
1:165649840:GA:G	acceptor_gain	0.9800
1:165649840:GACG:G	acceptor_gain	0.9800

AlphaMissense

972 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:165649899:A:C	S18R	0.998
1:165649901:C:A	S18R	0.998
1:165649901:C:G	S18R	0.998
1:165649944:C:A	R33S	0.993
1:165651056:T:C	F54L	0.992
1:165651058:C:A	F54L	0.992
1:165651058:C:G	F54L	0.992
1:165651072:G:C	R59P	0.990
1:165654319:G:C	R97P	0.990
1:165651081:A:C	Q62P	0.989
1:165649945:G:C	R33P	0.988
1:165651057:T:C	F54S	0.987
1:165651070:G:C	Q58H	0.987
1:165651070:G:T	Q58H	0.987
1:165649891:G:A	G15D	0.986
1:165651061:C:A	N55K	0.986
1:165651061:C:G	N55K	0.986
1:165654297:G:C	G90R	0.985
1:165649890:G:C	G15R	0.984
1:165651017:C:T	P41S	0.984
1:165651064:C:G	C56W	0.983
1:165651018:C:A	P41H	0.982
1:165654298:G:A	G90D	0.982
1:165654306:T:A	W93R	0.982
1:165654306:T:C	W93R	0.982
1:165649941:G:C	A32P	0.981
1:165651983:A:T	E66V	0.981
1:165651984:A:C	E66D	0.981
1:165651984:A:T	E66D	0.981
1:165651057:T:G	F54C	0.980

dbSNP variants (sampled 300 via entrez): RS1000006321 (1:165643683 T>G), RS1000032957 (1:165655466 A>G), RS1000059631 (1:165649270 T>C), RS1000091665 (1:165649042 A>C), RS1000105771 (1:165637083 A>G), RS1000195684 (1:165631732 T>A), RS1000385728 (1:165655113 C>T), RS1000734590 (1:165637732 C>T), RS1000785325 (1:165638046 G>A), RS1000794423 (1:165656441 T>C), RS1001034801 (1:165644701 T>A), RS1001070906 (1:165643574 A>T), RS1001147760 (1:165650302 G>T), RS1001183416 (1:165642996 A>G), RS1001402201 (1:165643472 A>G)

Disease associations

OMIM: gene MIM:604564 | disease phenotypes:

GenCC curated gene-disease

Mondo (1): pulmonary disease, chronic obstructive, susceptibility to (MONDO:0100167)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST000898_1	Total ventricular volume	7.000000e-06
GCST009391_233	Metabolite levels	4.000000e-06
GCST009391_988	Metabolite levels	2.000000e-06

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0010493	glycodeoxycholate measurement
EFO:0010475	deoxycholate measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL1743186 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

51 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects expression, decreases methylation, increases expression	6
sodium arsenite	decreases expression, increases abundance, increases expression	4
bisphenol A	affects expression, increases expression	2
TAK-243	decreases sumoylation	1
dicrotophos	decreases expression	1
bismuth tripotassium dicitrate	increases expression	1
methylmercuric chloride	increases expression	1
pirinixic acid	affects binding, increases activity, increases expression	1
arsenite	affects binding, increases reaction	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
W 7	decreases reaction, increases expression	1
perfluorooctanoic acid	increases expression	1
dinophysistoxin 1	increases expression	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	affects expression	1
bisphenol B	increases expression	1
abrine	increases expression	1
jinfukang	increases expression	1
bisphenol AF	increases expression	1
Temozolomide	decreases expression	1
Sunitinib	decreases expression	1
Troglitazone	increases expression	1
Artesunate	affects response to substance	1
Leflunomide	decreases expression	1
Aerosols	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	decreases expression, increases abundance	1
Benzo(a)pyrene	decreases methylation	1
Chelating Agents	affects binding, increases expression	1
Copper	increases expression, affects binding	1

ChEMBL screening assays

10 unique, capped per target: 9 binding, 1 admet

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1743241	ADMET	Substrates for human microsomal glutathione transferase MGST3	Casarett and Doull’s Toxicology The Basic Science of Poisons, 7th edition
CHEMBL4341385	Binding	Binding affinity to MGST3 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis	Profiling withanolide A for therapeutic targets in neurodegenerative diseases. — Bioorg Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.