MILIP

Summary

MILIP (MYC inducible lncRNA inactivating p53, HGNC:43649) is a long non-coding RNA gene on chromosome 17q25.3.

At a glance

• Gene type: non-coding (lncRNA) — no protein product.
• GTEx eGene: eQTL in 17 tissues, sQTL in 0 (q < 0.05)

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:43649
Approved symbol	MILIP
Name	MYC inducible lncRNA inactivating p53
Location	17q25.3
Locus type	RNA, long non-coding
Status	Approved
Ensembl gene	ENSG00000265688
Ensembl biotype	lncRNA
OMIM	621176
Entrez	92659
RNAcentral	URS000075B20D — lncRNA, 1914 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 lncRNA

Transcript support level (Ensembl TSL; 1 = fully mRNA-supported): TSL 1: 1

ENST00000582106

RefSeq mRNA: 0

Canonical transcript exons

ENST00000582106 — 2 exons

Exon	Start	End
ENSE00002690534	81929476	81930753
ENSE00002713444	81927829	81928445

Expression profiles

Bgee: expression breadth ubiquitous, 156 present calls, max score 79.53.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.8218 / max 54.1666, expressed in 1394 samples.

Highest expression (Bgee): primordial germ cell in gonad, secondary oocyte, male germ line stem cell (sensu Vertebrata) in testis, oocyte, stromal cell of endometrium.

Genetic regulation across tissues (GTEx eQTL / sQTL)

MILIP is an eGene in GTEx v10 — its expression is genetically regulated (eQTL) in 17 tissues and its splicing (sQTL) in 0 (q < 0.05). Strongest associations first; slope = normalized effect of the lead variant’s alternative allele.

17 significant tissue × QTL associations:

Tissue	QTL	q-value	Slope	Lead variant
Spleen	eqtl	8.9e-12	+2.26	17:81927264:G:A
Brain Substantia nigra	eqtl	8.4e-11	-0.63	17:81951661:G:A
Cells Cultured fibroblasts	eqtl	2.8e-07	+0.63	17:81927853:C:A
Cells EBV-transformed lymphocytes	eqtl	2.4e-05	+1.49	17:81927853:C:A
Artery Aorta	eqtl	1.0e-04	+0.78	17:81927853:C:A
Brain Cerebellar Hemisphere	eqtl	2.6e-04	-0.22	17:81943789:T:G
Nerve Tibial	eqtl	3.6e-04	+0.64	17:81927264:G:A
Muscle Skeletal	eqtl	1.1e-03	+0.62	17:81927853:C:A
Adrenal Gland	eqtl	1.3e-03	+0.98	17:81927853:C:A
Heart Left Ventricle	eqtl	1.4e-03	+1.15	17:81927264:G:A
Adipose Subcutaneous	eqtl	1.9e-03	+0.74	17:81936383:A:G
Prostate	eqtl	8.0e-03	-1.10	17:82049082:T:C
Artery Tibial	eqtl	1.3e-02	+0.63	17:81927853:C:A
Brain Hypothalamus	eqtl	2.4e-02	-0.25	17:81946986:G:GTAATAA
Brain Cerebellum	eqtl	4.0e-02	-0.19	17:81961589:A:C
Whole Blood	eqtl	4.2e-02	+0.79	17:81936383:A:G
Brain Putamen basal ganglia	eqtl	4.9e-02	-0.62	17:81436179:C:T

FANTOM5 promoters

2 alternative TSS:

Promoter ID	TPM avg	Samples expressed
163383	2.3109	1158
208458	1.5108	866

Top tissues by expression

showing 100 of 230 tissues by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	79.53	gold quality
secondary oocyte	CL:0000655	78.77	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	76.92	gold quality
oocyte	CL:0000023	74.84	silver quality
stromal cell of endometrium	CL:0002255	74.62	gold quality
cortical plate	UBERON:0005343	72.73	gold quality
adenohypophysis	UBERON:0002196	72.48	gold quality
ventricular zone	UBERON:0003053	72.27	gold quality
parotid gland	UBERON:0001831	71.96	gold quality
buccal mucosa cell	CL:0002336	71.68	gold quality
pituitary gland	UBERON:0000007	71.51	gold quality
right testis	UBERON:0004534	70.12	gold quality
left testis	UBERON:0004533	70.07	gold quality
right uterine tube	UBERON:0001302	68.77	gold quality
testis	UBERON:0000473	68.63	gold quality
ganglionic eminence	UBERON:0004023	66.56	gold quality
bronchial epithelial cell	CL:0002328	65.89	silver quality
bronchus	UBERON:0002185	65.38	silver quality
lower esophagus mucosa	UBERON:0035834	64.83	gold quality
tendon of biceps brachii	UBERON:0008188	64.19	gold quality
nucleus accumbens	UBERON:0001882	63.90	gold quality
esophagus mucosa	UBERON:0002469	63.75	gold quality
prefrontal cortex	UBERON:0000451	63.69	gold quality
cartilage tissue	UBERON:0002418	63.68	gold quality
left uterine tube	UBERON:0001303	63.45	gold quality
body of pancreas	UBERON:0001150	62.57	gold quality
right lobe of thyroid gland	UBERON:0001119	62.55	gold quality
anterior cingulate cortex	UBERON:0009835	62.48	gold quality
caudate nucleus	UBERON:0001873	62.44	gold quality
ectocervix	UBERON:0012249	62.44	gold quality
neocortex	UBERON:0001950	62.40	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	62.34	gold quality
hindlimb stylopod muscle	UBERON:0004252	62.27	gold quality
forebrain	UBERON:0001890	62.21	gold quality
frontal cortex	UBERON:0001870	62.18	gold quality
islet of Langerhans	UBERON:0000006	62.01	gold quality
putamen	UBERON:0001874	61.91	gold quality
pancreas	UBERON:0001264	61.81	gold quality
mammary duct	UBERON:0001765	61.80	gold quality
uterine cervix	UBERON:0000002	61.77	gold quality
Brodmann (1909) area 9	UBERON:0013540	61.71	gold quality
right frontal lobe	UBERON:0002810	61.65	gold quality
cortex of kidney	UBERON:0001225	61.62	gold quality
saliva-secreting gland	UBERON:0001044	61.57	gold quality
brain	UBERON:0000955	61.52	gold quality
myocardium	UBERON:0002349	61.45	gold quality
cerebral cortex	UBERON:0000956	61.27	gold quality
nasal cavity mucosa	UBERON:0001826	61.22	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	61.20	gold quality
endocervix	UBERON:0000458	61.10	gold quality
body of uterus	UBERON:0009853	61.02	gold quality
hypothalamus	UBERON:0001898	60.98	gold quality
adult mammalian kidney	UBERON:0000082	60.97	gold quality
esophagus	UBERON:0001043	60.81	gold quality
superior frontal gyrus	UBERON:0002661	60.78	gold quality
left lobe of thyroid gland	UBERON:0001120	60.72	gold quality
amygdala	UBERON:0001876	60.61	gold quality
esophagus squamous epithelium	UBERON:0006920	60.12	gold quality
minor salivary gland	UBERON:0001830	60.10	gold quality
temporal lobe	UBERON:0001871	60.02	gold quality
fallopian tube	UBERON:0003889	59.97	gold quality
seminal vesicle	UBERON:0000998	59.91	silver quality
thyroid gland	UBERON:0002046	59.86	gold quality
myometrium	UBERON:0001296	59.73	gold quality
apex of heart	UBERON:0002098	59.63	gold quality
metanephros cortex	UBERON:0010533	59.42	gold quality
mouth mucosa	UBERON:0003729	59.21	gold quality
upper lobe of left lung	UBERON:0008952	59.09	gold quality
right hemisphere of cerebellum	UBERON:0014890	58.95	gold quality
Ammon’s horn	UBERON:0001954	58.89	gold quality
metanephros	UBERON:0000081	58.85	gold quality
substantia nigra	UBERON:0002038	58.76	gold quality
cerebellum	UBERON:0002037	58.73	gold quality
primary visual cortex	UBERON:0002436	58.72	gold quality
cerebellar cortex	UBERON:0002129	58.68	gold quality
cerebellar hemisphere	UBERON:0002245	58.64	gold quality
upper lobe of lung	UBERON:0008948	58.55	gold quality
ascending aorta	UBERON:0001496	58.52	gold quality
skin of abdomen	UBERON:0001416	58.33	gold quality
thoracic aorta	UBERON:0001515	58.30	gold quality
gall bladder	UBERON:0002110	58.23	gold quality
midbrain	UBERON:0001891	58.15	gold quality
kidney	UBERON:0002113	58.11	gold quality
pigmented layer of retina	UBERON:0001782	58.07	gold quality
skin of leg	UBERON:0001511	57.97	gold quality
left adrenal gland cortex	UBERON:0035825	57.97	gold quality
smooth muscle tissue	UBERON:0001135	57.95	gold quality
left adrenal gland	UBERON:0001234	57.95	gold quality
occipital lobe	UBERON:0002021	57.82	gold quality
lower esophagus	UBERON:0013473	57.68	gold quality
right ovary	UBERON:0002118	57.66	gold quality
lower esophagus muscularis layer	UBERON:0035833	57.62	gold quality
prostate gland	UBERON:0002367	57.43	gold quality
adrenal cortex	UBERON:0001235	57.37	gold quality
vagina	UBERON:0000996	57.36	gold quality
zone of skin	UBERON:0000014	57.30	gold quality
body of stomach	UBERON:0001161	57.29	gold quality
anatomical system	UBERON:0000467	57.02	gold quality
cerebellar vermis	UBERON:0004720	56.99	gold quality
upper leg skin	UBERON:0004262	56.90	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.98

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 16)

• suggested that MAFG-AS1 functions as a novel oncogenic lncRNA in the development of CRC by regulating miR-147b/NDUFA4 (PMID:30348529)
• LncRNA MAFG-AS1 promotes the aggressiveness of breast carcinoma through regulating miR-339-5p/MMP15. (PMID:31002134)
• LncRNA MAFG-AS1 boosts the proliferation of lung adenocarcinoma cells via regulating miR-744-5p/MAFG axis (PMID:31211984)
• MAFG-AS1 is a novel clinical biomarker for clinical progression and unfavorable prognosis in gastric cancer. (PMID:32079456)
• Long noncoding RNA MAFG-AS1 facilitates the progression of hepatocellular carcinoma via targeting miR-3196/OTX1 axis. (PMID:33336731)
• Regulatory effect of the MAFGAS1/miR1505p/MYB axis on the proliferation and migration of breast cancer cells. (PMID:33367930)
• LncRNA MAFG-AS1 regulates miR-125b-5p/SphK1 axis to promote the proliferation, migration, and invasion of bladder cancer cells. (PMID:33400245)
• LncRNA MAFG-AS1 affects the tumorigenesis of breast cancer cells via the miR-574-5p/SOD2 axis. (PMID:33989902)
• LncRNA MAFG-AS1 promotes the malignant phenotype of ovarian cancer by upregulating NFKB1-dependent IGF1. (PMID:34035482)
• LncRNA MAFG-AS1 deregulated in breast cancer affects autophagy and progression of breast cancer by interacting with miR-3612 and FKBP4 invitro. (PMID:35653827)
• LncRNA MILIP links YBX1 to translational activation of Snai1 and promotes metastasis in clear cell renal cell carcinoma. (PMID:36028903)
• LncRNA MAFG-AS1-induced acute myeloid leukemia development via modulating miR-147b/HOXA9. (PMID:36229729)
• The N-Myc-responsive lncRNA MILIP promotes DNA double-strand break repair through non-homologous end joining. (PMID:36445966)
• MAFG-AS1/MAFG positive feedback loop contributes to cisplatin resistance in bladder urothelial carcinoma through antagonistic ferroptosis. (PMID:36654385)
• A review on the roles and molecular mechanisms of MAFG-AS1 in oncogenesis. (PMID:36736142)
• Long non-coding RNA MAFG-AS1: A promising therapeutic target for human cancers. (PMID:37105079)

Cross-species orthologs

0 orthologs

Genomic neighbors (locus context)

Genes flanking this one on the chromosome (NCBI/Entrez gene neighborhood). Positional context only — some neighbors share regulatory elements (bidirectional promoters, antisense/overlapping transcripts), but chromosomal adjacency is not itself a functional relationship (see Interactions for that).

Symbol	Type	Entrez
MAFG-AS1	ncRNA	128385369
NPB	protein-coding	256933
MAFG	protein-coding	4097
SIRT7	protein-coding	51547
PYCR1	protein-coding	5831
PCYT2	protein-coding	5833
ALYREF	protein-coding	10189
ANAPC11	protein-coding	51529
MYADML2	protein-coding	255275
NOTUM	protein-coding	147111

Protein

Non-coding RNA — no protein product.

Function

Gene-set membership (MSigDB)

This non-coding RNA is a member of 26 curated MSigDB gene sets — regulatory-target and signature collections that include it (it has no protein-based pathway / GO / interaction data).

GARGALOVIC_RESPONSE_TO_OXIDIZED_PHOSPHOLIPIDS_BLUE_UP, LIAO_METASTASIS, E2F3_UP.V1_UP, MAFG_TARGET_GENES, NCOA6_TARGET_GENES, RBM34_TARGET_GENES, TFEB_TARGET_GENES, ZBTB12_TARGET_GENES, ZFP91_TARGET_GENES, ZNF354B_TARGET_GENES, ZNF410_TARGET_GENES, ZNF423_TARGET_GENES, ZNF507_TARGET_GENES, ZNF561_TARGET_GENES, ZNF830_TARGET_GENES, ZSCAN30_TARGET_GENES, BLANCO_MELO_INFLUENZA_A_INFECTION_A594_CELLS_DN, AHRR_TARGET_GENES, MZF1_TARGET_GENES, ZNF549_TARGET_GENES, MYOCD_TARGET_GENES, TRAVAGLINI_LUNG_PROXIMAL_CILIATED_CELL, CARRILLOREIXACH_HEPATOBLASTOMA_VS_NORMAL_UP, GSE2706_R848_VS_LPS_2H_STIM_DC_DN, chr17q25, GSE41867_DAY6_VS_DAY15_LCMV_ARMSTRONG_EFFECTOR_CD8_TCELL_DN

ncRNA interactions

Experimentally supported non-coding-RNA interaction partners (NPInter).

showing 4 of 50 interaction partners:

Partner	Partner type	Level	Source
MAFG-AS1	lncRNA	RNA-Protein	High-throughput data
MAFG-DT	lncRNA	RNA-Protein	High-throughput data
miR-125b-5p	miRNA	RNA-RNA	Literature mining
miR-143-3p	miRNA	RNA-RNA	Literature mining

Disease & clinical

ncRNA disease associations

Curated non-coding-RNA → disease associations (LncRNADisease, HMDD) — these are RNA-level associations for this gene, not the positional variant data omitted above.

showing 19 of 27 ncRNA–disease associations:

Disease	Causal?	Validated by
Carcinoma, Hepatocellular	No	qRT-PCR
Ovarian Neoplasms	Yes	Transwell Assay, Wound Healing Assay, qRT-PCR, FISH, RIP, Colony Formation Assay, ChIP
Carcinoma, Hepatocellular	Yes	Western Blot, CCK8, qRT-PCR, RIP, Luciferase Report Assay, Transwell Assay
Esophageal Squamous Cell Carcinoma	Yes	RNA Pull-Down, Western Blot, qRT-PCR, Luciferase Report Assay, Transwell Assay
Prostatic Neoplasms	Yes	Invasion Assay, Migration Assay, qRT-PCR, RNA-seq, Cell Proliferation Assay, Colony Formation Assay, Bioinformatics Analysis, Transwell Assay
Carcinoma, Non-Small-Cell Lung	Yes	qRT-PCR
Breast Neoplasms	Yes	RNA Pull-Down, CCK8, qRT-PCR, RIP, Luciferase Report Assay, BrdU, ELISA
Leukemia, Myeloid, Acute	Yes	qRT-PCR, Luciferase Report Assay
Urinary Bladder Neoplasms	Yes	qRT-PCR, Wound Healing Assay
Colorectal Neoplasms	Yes	Western Blot, qRT-PCR, MTT, Luciferase Report Assay, Transwell Assay
Glioblastoma	Yes	Western Blot, Wound Healing Assay, CCK8, qRT-PCR, Flow Cytometry, Colony Formation Assay
Breast Neoplasms	No	CCK8, qRT-PCR, Invasion Assay, Migration Assay
gastric adenocarcinoma	Yes	CCK8, qRT-PCR, Luciferase Report Assay, Western Blot
bladder urothelial carcinoma	Yes	IHC, Western Blot, Wound Healing Assay, Co-IP, qRT-PCR, FISH, RIP, MTT, IP, Colony Formation Assay, Transwell Assay
Carcinoma, Renal Cell	Yes	In Vivo Experiment, RNA Pull-Down, Western Blot, Wound Healing Assay, Migration Assay, qRT-PCR, RIP, Invasion Assay, Transwell Assay
Stomach Neoplasms	Yes	qRT-PCR
Adenocarcinoma of Lung	Yes	CCK8, FISH, EdU Staining, Western Blot
Colorectal Neoplasms	No	qRT-PCR
Urinary Bladder Neoplasms	No	qRT-PCR, Luciferase Report Assay, RIP

Drugs & pharmacology

ncRNA–drug associations

Curated non-coding-RNA → drug associations (drug response / resistance / target).

showing 5 of 7 ncRNA–drug associations:

Drug	Relation	Effect	Condition
Oxaliplatin	drug_resistance	sensitive	cell line (MHCC97H)
Gemcitabine	drug_resistance	resistant	“cell line (HuCCT1, SNU-245)”
Tamoxifen	drug_resistance	resistant	“cell line (MCF-7, BT-474, T47D, MCF-10A)”
Oxaliplatin	drug_target	down-regulated	cell line (MHCC97H)
Panobinostat	drug_target	down-regulated	“cell line (SK-NEP-1, G401)”