MIR101-1

Summary

MIR101-1 (microRNA 101-1, HGNC:31488) is a microRNA gene on chromosome 1p31.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406893 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31488
Approved symbol	MIR101-1
Name	microRNA 101-1
Location	1p31.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-101-1
Ensembl gene	ENSG00000199135
Ensembl biotype	miRNA
OMIM	612511
Entrez	406893
RNAcentral	URS0000370AC5 — miRNA, 75 nt, 7 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362265

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362265 — 1 exons

Exon	Start	End
ENSE00001437028	65058434	65058508

Expression profiles

Bgee: expression breadth broad, 73 present calls, max score 92.77.

Top tissues by expression

73 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	92.77	gold quality
sural nerve	UBERON:0015488	86.65	gold quality
blood	UBERON:0000178	83.67	gold quality
bone marrow	UBERON:0002371	82.07	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	81.46	gold quality
kidney	UBERON:0002113	77.96	gold quality
placenta	UBERON:0001987	77.81	gold quality
muscle of leg	UBERON:0001383	76.53	gold quality
endometrium	UBERON:0001295	76.16	gold quality
monocyte	CL:0000576	76.04	gold quality
gastrocnemius	UBERON:0001388	72.96	gold quality
islet of Langerhans	UBERON:0000006	72.72	gold quality
substantia nigra	UBERON:0002038	72.66	gold quality
heart	UBERON:0000948	72.33	gold quality
body of pancreas	UBERON:0001150	71.24	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	70.62	gold quality
popliteal artery	UBERON:0002250	69.96	gold quality
tibial artery	UBERON:0007610	69.86	gold quality
anterior cingulate cortex	UBERON:0009835	69.17	gold quality
stomach	UBERON:0000945	69.04	gold quality
ovary	UBERON:0000992	68.60	gold quality
left adrenal gland cortex	UBERON:0035825	68.57	gold quality
left ovary	UBERON:0002119	68.25	gold quality
right atrium auricular region	UBERON:0006631	67.76	gold quality
right ovary	UBERON:0002118	67.46	gold quality
urinary bladder	UBERON:0001255	67.29	gold quality
adrenal gland	UBERON:0002369	67.26	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.99	gold quality
lung	UBERON:0002048	66.98	gold quality
myometrium	UBERON:0001296	66.55	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.75

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): AR, ARNT, HIF1A

Literature-anchored findings (GeneRIF, showing 40)

• study proposes that the genomic loss of miR-101 in cancer leads to overexpression of EZH2 and concomitant dysregulation of epigenetic pathways, resulting in cancer progression (PMID:19008416)
• MicroRNA-101, which is aberrantly expressed in hepatocellular carcinoma, could repress the expression of the FOS oncogene. (PMID:19133651)
• These results not only establish a role for miR-101 in estrogen-independent signaling but also provide a mechanistic link between miR-101 and Akt activation. (PMID:20956939)
• endogenous miR-101 regulates expression of APP in human cells via a specific site located within its 3’-UTR. (PMID:21172309)
• Data show there was an inverse correlation between the expression level of EZH2 and that of miR-101 or miR-128a, suggesting that the altered expression of the latter miRNAs accounts for the overexpression of the former. (PMID:21228036)
• Reduced expression of miR-101 was associated with overexpression of EZH2 in non-small cell lung cancer. (PMID:21270667)
• Up-regulation of miR-101 expression may play a role in repressing productive HSV-1 replication by targeting ATP5B. (PMID:21291913)
• by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process (PMID:21297974)
• EZH2 has a versatile function in glioblastoma progression and that its overexpression is at least partly due to decreased miR-101 expression. (PMID:21321380)
• A polycomb-mediated repression of rap1GAP was demonstrated that involves EZH2, a histone methyltransferase in head and neck cancers. It was also shown that the loss of miR-101 expression correlates with EZH2 upregulation and rap1GAP downregulation. (PMID:21532618)
• The tumour-suppressor miRNAs let-7 and mir-101 target MYCN and inhibit proliferation and clonogenic growth of MYCN-amplified neuroblastoma cells. (PMID:21654684)
• miR-101 DNA copy loss is a prominent subtype specific event in lung cancer. (PMID:21849855)
• identified the tumour suppressive miRNA, miR-101, as a potent inhibitor of basal, etoposide- and rapamycin-induced autophagy (PMID:21915098)
• these results show nuclear EZH2 as a prognostic marker of worse survival in human RCC, and identify miR-101 as a negative regulator of EZH2 expression and renal cancer cell proliferation. (PMID:22609199)
• The misregulation of hsa-miR-101 contributes partly to the overexpression of p23 in childhood acute lymphoblastic leukemia. (PMID:22677230)
• upregulation of miR-101 in response to laminar shear stress (LSS) contributes to the suppressive effects of LSS on mTOR expression and endothelial cells proliferation. (PMID:22989749)
• Downregulation of miR-101 in gastric cancer correlates with cyclooxygenase-2 overexpression and gastric tumor growth. (PMID:23013439)
• study demonstrates that down-regulation of miR-101 in different subtypes of human breast cancer tissues is linked to the increase of cellular proliferation and invasiveness via targeting Stmn1 (PMID:23071542)
• MiR-101 is another important component of the MAPK signaling pathway affects the secretion of the downstream inflammatory cytokines in systemic lupus erythematosis. (PMID:23139385)
• miR-101 may suppress human hepatocellular carcinoma tumor progression by down-regulating SOX9 (PMID:23178713)
• These results suggest that chronic cigarette smoking up-regulates miR-101 and that this miRNA could contribute to suppression of CFTR in the lungs of chronic obstructive pulmonary disease patients. (PMID:23226399)
• miR-101 and Ezh2 are key players in UVB-induced senescence of human diploid fibroblasts. (PMID:23557329)
• The VEGF and COX-2 mRNAs were identified as the bona fide targets of miR-101 in cholangiocarcinoma cells. (PMID:23608225)
• Data indicate that miR-101 directly targets c-Met via its 3’-UTR. (PMID:23618864)
• MiR-101 downregulated the expression of CPEB1 through reversing the methylation status of the CPEB1 promoter. (PMID:23788032)
• Data indicate that miR-101 overexpression downregulates microphthalmia-associated transcription factor (MITF) and enhancer of zeste homolog 2 (EZH2) protein in melanoma cells. (PMID:23962556)
• further increases expression of endogenous miR-101 by inhibition of PRC2 activation (PMID:24002871)
• Myeloid-derived suppressor cells triggered miRNA101 expression in cancer cells. miRNA101 subsequently repressesed the corepressor gene C-terminal binding protein-2 (CtBP2). (PMID:24012420)
• There was a negative correlation between expression levels of miR-101 and EZH2 (PMID:24211739)
• Low expression of microRNA-101 is associated with cervical carcinoma. (PMID:24289600)
• This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs. (PMID:24412053)
• miR-101 can directly target ZEB1 and ZEB2, resulting in suppression of the EMT in ovarian carcinoma. (PMID:24677166)
• Hepatitis B virus can downregulate miR-101-3p expression by inhibiting its promoter activity and that downregulation of miR-101-3p promotes hepatocellular carcinoma cell proliferation and migration by targeting Rab5a. (PMID:24788845)
• findings indicated that overexpression of miR-101 could downregulate ERp44 (PMID:24804790)
• Data showed that miR-101 was downregulated and its target EZH2 was upregulated in retinoblastoma tissues and ther expression is significantly associated with adverse clinicopathological and histopathological features. (PMID:24807198)
• The expression of miR-101 is down-regulated in bladder transitional cell carcinoma. (PMID:24834983)
• By targeting the proto-oncogene Fos, miR-101 is involved in G1-to-S phase transition in cervical cancer cells in vitro. (PMID:24987920)
• Downregulation of miR101 induced cell survival and cisplatin resistance through the upregulation of COX2 expression. (PMID:25109742)
• miR-101 and miR-145 are specifically upregulated in adult lung and miR-101 directly acts on its cognate site in the CFTR-3’UTR in combination with an overlapping AU-rich element (PMID:25186262)
• Results indicate that miR-101 may act as a tumor suppressor in osteosarcoma, as it has a suppressive role in cell migration and invasion by targeting EZH2. (PMID:25190211)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir101a	ENSDARG00000082131
mus_musculus	Mir101a	ENSMUSG00000065451

Paralogs (1): MIR101-2 (ENSG00000199065)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.