MIR10B

Summary

MIR10B (microRNA 10b, HGNC:31498) is a microRNA gene on chromosome 2q31.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406903 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31498
Approved symbol	MIR10B
Name	microRNA 10b
Location	2q31.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-10b
Ensembl gene	ENSG00000207744
Ensembl biotype	miRNA
OMIM	611576
Entrez	406903
RNAcentral	URS00004CAC40 — miRNA, 110 nt, 4 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385011

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385011 — 1 exons

Exon	Start	End
ENSE00002719037	176150303	176150412

Expression profiles

Bgee: expression breadth broad, 59 present calls, max score 81.69.

Top tissues by expression

59 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	81.69	gold quality
heart left ventricle	UBERON:0002084	79.95	gold quality
heart	UBERON:0000948	75.52	gold quality
sural nerve	UBERON:0015488	74.62	gold quality
muscle of leg	UBERON:0001383	73.88	gold quality
monocyte	CL:0000576	73.65	gold quality
epididymis	UBERON:0001301	72.40	gold quality
gastrocnemius	UBERON:0001388	72.35	gold quality
body of stomach	UBERON:0001161	72.22	gold quality
islet of Langerhans	UBERON:0000006	70.94	gold quality
adult mammalian kidney	UBERON:0000082	70.68	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	70.59	gold quality
right lobe of liver	UBERON:0001114	70.43	gold quality
body of uterus	UBERON:0009853	70.31	gold quality
esophagus mucosa	UBERON:0002469	70.27	gold quality
kidney	UBERON:0002113	69.85	gold quality
ascending aorta	UBERON:0001496	69.71	gold quality
tibial artery	UBERON:0007610	69.68	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	69.25	gold quality
myometrium	UBERON:0001296	69.23	gold quality
calcaneal tendon	UBERON:0003701	69.09	gold quality
left coronary artery	UBERON:0001626	68.93	gold quality
vagina	UBERON:0000996	68.43	gold quality
transverse colon	UBERON:0001157	68.20	gold quality
uterus	UBERON:0000995	67.90	gold quality
skin of abdomen	UBERON:0001416	67.59	gold quality
colon	UBERON:0001155	67.24	gold quality
omental fat pad	UBERON:0010414	67.15	gold quality
right ovary	UBERON:0002118	67.04	gold quality
right adrenal gland cortex	UBERON:0035827	67.00	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.22

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• microRNA-10b (miR-10b) is highly expressed in metastatic breast cancer cells and positively regulates cell migration and invasion (PMID:17898713)
• MicroRNA-10b is overexpressed in malignant glioma and associated with tumor invasive factors, uPAR and RhoC. (PMID:19536818)
• Identified the effect of post-transcriptional regulation of miRNA-10b on PPAR-alpha expression to influence steatosis level in hepatocytes, which may contribute to pathogenesis of non-alcoholic fatty liver disease. (PMID:19780876)
• elevated expression of miR-10b in 95% (38 of 40) of esophageal cancer tissues, although no significant correlation of the miR-10b level with clinical metastasis status was observed in these samples (PMID:20075075)
• Data show that NF1 mRNA was the target for miR-10b. (PMID:20550523)
• miR-10b positively regulates the metastasis of nasopharyngeal carcinoma (NPC). (PMID:20732742)
• Hyaluronan-CD44 interaction promotes c-Src-mediated twist signaling, microRNA-10b expression, and RhoA/RhoC up-regulation, leading to Rho-kinase-associated cytoskeleton activation and breast tumor cell invasion. (PMID:20843787)
• changes in miR-10a and -10b expression levels may regulate SFRS1-dependent alternative splicing and translational functions. (PMID:21118818)
• The miR-10b has major roles in the process of neural cell differentiation through direct targeting of NCOR2, which in turn induces a cascade of primary and secondary transcriptional alterations. (PMID:21212796)
• Data show that Argonaute 2 expression is critical for stem cells to escape senescence by downregulating miR10b and miR23b, and that selenoprotein N1 is also involved in ATSC survival and self-renewal through ROS-mediated p38 MAPK inactivation. (PMID:21241449)
• miR-10b induced glioma cell invasion by modulating tumor invasion factors MMP-14 and uPAR expression via the direct target HOXD10 (PMID:21419107)
• microRNA-10b has a role in glioma (PMID:21471404)
• report that the microRNA-10b gene (miR-10b) was silenced in gastric cancer cells by promoter methylation (PMID:21562367)
• heparin attenuates miR-10b expression and induces HoxD10 expression in vivo to inhibit angiogenesis and impair the growth of MDA-MB-231 tumor xenografts (PMID:21642433)
• in 10 pancreatic ductal adenocarcinoma (PDAC) samples, miR-10b was the most frequently overexpressed miRNA exhibiting a 4-fold increase in cancer cells; lower levels were associated with improved response to multimodality neoadjuvant therapy and survival (PMID:21652542)
• We experimentally validated the role of one of the dysregulated miRNAs (miR-10b) in relapse using proliferation and wound-healing assay. (PMID:21769427)
• MIR-10b is overexpressed in pancreatic cancer and may be involved in the invasiveness in pancreatic cancer cells, thereby leading to a poor prognosis. (PMID:22018284)
• WISP-2 regulates microRNA-10b via hypoxia-inducible factor-1alpha-TWIST signaling networks in human breast cancer cells. (PMID:22020939)
• miR-10b showed higher expression levels in tumors obtained from bilateral breast carcinoma patients as compared to unilateral neoplasms. (PMID:22057972)
• Suggest that miR-10b can stimulate the upregulation of RhoC and AKT phosphorylation through targeting HOXD10, thus promoting cell invasion in gastric tumors. (PMID:22293682)
• miR-10b actively participates in cancer formation by promoting cell migration and invasion (PMID:22318752)
• We have demonstrated that high expression of miR-10b is associated with enhanced malignant potential and poor prognosis in human colorectal cancer. In vitro studies revealed that miR-10b expression is associated with chemoresistance to 5-fluorouracil. (PMID:22322955)
• miR-10b may positively regulate the invasion and metastasis of HCC through targeting CADM1. (PMID:22528944)
• Rho-GTPase-dependent modulation of cytoskeletal function and downregulation of E-cadherin expression are identified as relevant effectors of the miR-10b-syndecan-1 axis (PMID:22573479)
• Treatment after the formation of lymph node metastases arrests the metastatic process without a concomitant effect on primary tumor growth raising the possibility of a context-dependent variation in miR-10b breast oncogenesis (PMID:22580603)
• we found that both miR-10b and miR-196b show elevated expression in human high-grade breast tumor vasculature (PMID:22836757)
• These findings indicate the existence of a novel E-cadherin-related mechanism by which miR-10b modulates breast cancer metastasis. (PMID:22847191)
• our results define MICB as a novel immune target of miR-10b (PMID:22915757)
• TWIST-1 and miR10a/b expression are dysregulated in MDS. Expression of miRs10a/b is controlled by TWIST-1. (PMID:22983574)
• The pleiotropic nature of miRNA-10b was due to its suppression of multiple tumor suppressors, including TP53, FOXO3, CYLD, PAX6, PTCH1, HOXD10 and NOTCH1. (PMID:23034333)
• Ectopic delivery of microRNA-10b* in breast cancer cell lines or into xenograft mouse breast tumours inhibits cell proliferation and impairs tumour growth. (PMID:23125021)
• Syndecan-1, a target of miR-10b, inhibits epithelial endometriotic cell invasiveness through down-regulation of metalloproteinase activity and interleukin-6. (PMID:23206733)
• study observed that circulating miR-10b and miR-373 levels could distinguish breast cancer patients with lymph node metastasis from non-metastatic patients (PMID:23238818)
• High miR-10b levels are associated with glioblastoma. (PMID:23307328)
• In humans, miR-10b may play an important role in progression and prognosis of gastric cancer. (PMID:23351547)
• MiR-10b is highly upregulated in metastatic minimally invasive follicular thyroid carcinoma. (PMID:23563786)
• downregulation of HOXD10 expression by miR-10b overexpression may induce an increase of pro-metastatic gene products, such as MMP14 and RHOC, and contribute to the acquisition of metastatic phenotypes in epithelial ovarian cancer cells (PMID:23670532)
• The miR-10b locus and its involvement in cancer, focusing on its role in the establishment and spreading of breast cancer. [Review] (PMID:23839045)
• Identification of a possible link between miR-10b expression in metastasis and patient survival. (PMID:23987127)
• miR-10b promotes pancreatic cancer cell proliferation and invasion by suppressing TIP30. (PMID:24096486)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir10b-1	ENSDARG00000080641
danio_rerio	mir10d	ENSDARG00000083047
mus_musculus	Mir10b	ENSMUSG00000065500
rattus_norvegicus	Mir10b	ENSRNOG00000035554
drosophila_melanogaster	mir-10	FBGN0262424

Paralogs (7): MIR99B (ENSG00000207550), MIR99A (ENSG00000207638), MIR125B2 (ENSG00000207863), MIR125B1 (ENSG00000207971), MIR100 (ENSG00000207994), MIR125A (ENSG00000208008), MIR10A (ENSG00000284038)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.