MIR124-3

Summary

MIR124-3 (microRNA 124-3, HGNC:31504) is a microRNA gene on chromosome 20q13.33.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406909 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31504
Approved symbol	MIR124-3
Name	microRNA 124-3
Location	20q13.33
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-124a-3, hsa-mir-124-3
Ensembl gene	ENSG00000207598
Ensembl biotype	miRNA
Entrez	406909
RNAcentral	URS000075EDDE — miRNA, 87 nt, 38 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384866

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384866 — 1 exons

Exon	Start	End
ENSE00001499873	63178500	63178586

Expression profiles

Bgee: expression breadth broad, 36 present calls, max score 77.44.

Top tissues by expression

36 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
blood	UBERON:0000178	77.44	gold quality
putamen	UBERON:0001874	69.92	gold quality
body of pancreas	UBERON:0001150	68.11	gold quality
tibial artery	UBERON:0007610	68.09	gold quality
body of stomach	UBERON:0001161	67.70	gold quality
vagina	UBERON:0000996	67.50	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	67.29	gold quality
Brodmann (1909) area 9	UBERON:0013540	67.03	gold quality
anterior cingulate cortex	UBERON:0009835	66.40	gold quality
right atrium auricular region	UBERON:0006631	65.74	gold quality
subcutaneous adipose tissue	UBERON:0002190	65.56	gold quality
right frontal lobe	UBERON:0002810	65.01	gold quality
skin of leg	UBERON:0001511	64.93	gold quality
muscle layer of sigmoid colon	UBERON:0035805	63.58	gold quality
left ovary	UBERON:0002119	63.53	gold quality
right lung	UBERON:0002167	63.18	gold quality
esophagus mucosa	UBERON:0002469	63.14	gold quality
hypothalamus	UBERON:0001898	62.87	gold quality
thoracic mammary gland	UBERON:0005200	62.86	gold quality
right lobe of thyroid gland	UBERON:0001119	62.70	gold quality
caudate nucleus	UBERON:0001873	62.70	gold quality
lower esophagus muscularis layer	UBERON:0035833	62.54	gold quality
right hemisphere of cerebellum	UBERON:0014890	62.35	gold quality
body of uterus	UBERON:0009853	61.99	gold quality
transverse colon	UBERON:0001157	61.76	gold quality
omental fat pad	UBERON:0010414	61.62	gold quality
Ammon’s horn	UBERON:0001954	61.52	gold quality
spleen	UBERON:0002106	61.39	gold quality
tibial nerve	UBERON:0001323	61.15	gold quality
small intestine Peyer’s patch	UBERON:0003454	60.21	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.11

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. (PMID:19843643)
• Data suggest disruption of miR-124-mediated repression of ITGB1 may be a key factor in OSCC progression. (PMID:21112327)
• The methylation of mir-9-3, mir-124-2, and mir-124-3 was individually associated with an advanced T factor independent of age, sex, and smoking habit. (PMID:21917081)
• Methylation of mir-124-3 is suggested as an independent prognosticator for ccRCC. (PMID:23321515)
• we found that miR-124-1, miR-124-2 and miR-124-3 are highly methylated in pancreatic cancer tissues (PMID:23334332)
• miR-124-3p can repress the migration and invasion of bladder cancer cells via regulating ROCK1 (PMID:24180482)
• miR-124a-1, miR-124a-2, and miR-124a-3 genes are frequently methylated in breast cancer and play a role in tumor growth and aggressivity. (PMID:24375250)
• miR-124 and its target gene, SOX9, are overexpressed in the stenotic colon segment of patients with HSCR, and may have a significant role in the development of HSCR. (PMID:24604230)
• miR-124-3p and miR-16 are diagnostic markers to discriminate hemorrhagic and ischemic stroke (PMID:24650689)
• low expression of miR-124-3p, miR-128-1, and miR-221-3p may constitute a potential marker of astrocytomas that correlates with localization (PMID:24718706)
• Molecular data on gastric cance patients show that down regulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p is correlated with extensive lymph node metastasis, lymphatic abd venous invasion, high-stage Borrmann type and poor differentiation. (PMID:24805774)
• The methylation level of miR-124a-3 is a promising marker for estimating individual risk for colitis-associated cancer. (PMID:24825593)
• Findings identify that KITENIN-targeting miR-124, miR-27a, and miR-30b function as endogenous inhibitors of colorectal cancer cell motility and demonstrate that miR-124 plays a suppressor role in colorectal tumorigenesis. (PMID:24909917)
• MIRN124 binds to the 3’UTR of iASPP and suppressing mRNA expression and the proliferation of prostate tumor cells. (PMID:24966937)
• Findings suggest that miR-124 inhibits TGF-alpha-induced epithelial-mesenchymal transition in human prostate cancer cell line DU145 by targeting Slug. (PMID:24969691)
• A negative regulatory role of miR-124 in fine-tuning inflammatory response in alveolar macrophages upon mycobacterial infection, in part through a mechanism by directly targeting TLR signaling. (PMID:24995397)
• Results demonstrate that miR-124 functions as a tumor-suppressive microRNA in nasopharyngeal carcinoma by repressing Foxq1 expression. (PMID:25098939)
• miR-124 binds AmotL1 3’UTR and down-regulates its expression repressing vasculogenic mimicry and cell motility in cervical cancer cells. (PMID:25218344)
• Suggest that miRNA-124 may regulate non-small cell lung carcinoma cell proliferation via decreasing SOX8. (PMID:25400731)
• Data indicate differential expression of microRNAs miR-124 and let-7a between the smoking and control groups. (PMID:25598229)
• Suggest that miR-124 acts as a tumor-suppressor and a modulator of energy metabolism through a PTB1/PKM1/PKM2 feedback cascade in human colorectal tumor cells. (PMID:25818238)
• found a negative feedback loop between androgen receptor and miR-124 expression in prostate cancer cells (PMID:25860954)
• Decreased expression of miR-124 might be associated with tumor progression and poor prognosis in patients with breast cancer. (PMID:25924779)
• miR-124 regulates Tip110 expression and differentiation of human cord blood CD34(+) cells and suggests important roles of miR-124/Tip110 in hematopoiesis (PMID:25928721)
• miR-124 promotes hyperplasia and contributes to invasion of colorectal cancer cells, but downregulates ROCK1. (PMID:25987767)
• We identified the downregulated miR-124-3p, -30a-5p and -200c-3p as the most influential miRNAs in renal cell carcinoma pathogenesis (PMID:26002553)
• Paeoniflorin inhibits cell viability and induces apoptosis through the up-regulation of miR-124 and suppression of PI3K/Akt and STAT3 signaling in gastric carcinoma cells. (PMID:26109806)
• Overexpression of miR-124 decreased ESX and EGFR levels in head and neck squamous cell carcinoma cells and reduced cell invasion, migration, proliferation, and colony formation. (PMID:26227488)
• miR-124 could control the fate of target gene UHRF1 mRNA by binding 3’-UTR (PMID:26310391)
• miR-124 and miR-506 inhibit progression and increase sensitivity to chemotherapy by targeting DNMT3B and DNMT1 in colorectal carcinoma. (PMID:26497367)
• We found that miR-124 directly downregulates the levels of AR transcript variants, as well as enhancer of Zeste homolog 2 (EZH2) and Src tyrosine kinase (Src). (PMID:26573802)
• meta-analysis suggests that the miR-124 rs531564 C > G polymorphism is an important risk factor for cancers among the Chinese population (PMID:26625819)
• MiR-124 is an important miRNA modulating neurogenic transdifferentiation of ADMSCs at least partly via the miR-124/RhoA/ROCK1 signaling pathway. (PMID:26745800)
• data show a novel feedback loop between miR-124 and transforming growth factor-beta (TGF-beta) pathway driving non-small cell lung cancer (NSCLC) metastasis, which might provide a new insight into treatment of NSCLC progression (PMID:26818357)
• We demonstrated that a seed-modifying isomiR of the retina-enriched miR-124-3p was endowed with different targeting properties with respect to the corresponding canonical form (PMID:26819412)
• miR-124 functions as a tumor suppressor in lung adenocarcinoma by directly targeting SOX9. (PMID:26935152)
• High MIR124-3 expression is associated with acute stroke. (PMID:26968104)
• findings aid in understanding the tumor-suppressive role of miR-124-3p in astrocytoma pathogenesis through the inhibition of PIM1 translation (PMID:27088547)
• miR-124 inhibits lung cancer cell migration and invasion through suppressing epithelial-mesenchymal transition (EMT) and inducing apoptosis of the lung cancer cells.fa (PMID:27251409)
• The introduction of miR-214 significantly promoted the proliferation of pulmonary artery smooth muscle cells by suppressing cell apoptosis, and this effect was mediated by the downregulation of cyclin L2. (PMID:27381447)

Cross-species orthologs

8 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir124-5	ENSDARG00000080174
danio_rerio	dre-mir-124-1	ENSDARG00000080655
danio_rerio	mir124-6	ENSDARG00000083622
danio_rerio	dre-mir-124-4	ENSDARG00000107926
mus_musculus	Mir124a-3	ENSMUSG00000065454
rattus_norvegicus	Mir124-3	ENSRNOG00000035593
drosophila_melanogaster	mir-124	FBGN0262398
caenorhabditis_elegans		WBGENE00003319

Paralogs (2): MIR124-2 (ENSG00000207816), MIR124-1 (ENSG00000284321)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.