MIR128-2

Summary

MIR128-2 (microRNA 128-2, HGNC:31511) is a microRNA gene on chromosome 3p22.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406916 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31511
Approved symbol	MIR128-2
Name	microRNA 128-2
Location	3p22.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-128b, hsa-mir-128-2
Ensembl gene	ENSG00000207625
Ensembl biotype	miRNA
OMIM	611769
Entrez	406916
RNAcentral	URS000046FA49 — miRNA, 84 nt, 46 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384893

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384893 — 1 exons

Exon	Start	End
ENSE00001499900	35744476	35744559

Expression profiles

Bgee: expression breadth broad, 35 present calls, max score 87.95.

Top tissues by expression

35 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	87.95	gold quality
skeletal muscle tissue	UBERON:0001134	85.90	gold quality
stomach	UBERON:0000945	83.85	gold quality
muscle of leg	UBERON:0001383	78.88	gold quality
blood	UBERON:0000178	77.60	gold quality
gastrocnemius	UBERON:0001388	76.51	gold quality
esophagus mucosa	UBERON:0002469	70.90	gold quality
anterior cingulate cortex	UBERON:0009835	69.90	gold quality
adult mammalian kidney	UBERON:0000082	69.80	gold quality
right atrium auricular region	UBERON:0006631	69.00	gold quality
liver	UBERON:0002107	68.80	gold quality
transverse colon	UBERON:0001157	68.58	gold quality
cerebral cortex	UBERON:0000956	67.59	gold quality
Ammon’s horn	UBERON:0001954	67.39	gold quality
frontal cortex	UBERON:0001870	67.13	gold quality
amygdala	UBERON:0001876	67.04	gold quality
right frontal lobe	UBERON:0002810	65.90	gold quality
caudate nucleus	UBERON:0001873	65.56	gold quality
nucleus accumbens	UBERON:0001882	65.15	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	64.94	gold quality
putamen	UBERON:0001874	64.88	gold quality
right hemisphere of cerebellum	UBERON:0014890	64.24	gold quality
colon	UBERON:0001155	63.64	gold quality
ascending aorta	UBERON:0001496	63.48	gold quality
substantia nigra	UBERON:0002038	63.22	gold quality
vagina	UBERON:0000996	63.16	gold quality
hypothalamus	UBERON:0001898	62.91	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	62.77	gold quality
Brodmann (1909) area 9	UBERON:0013540	62.73	gold quality
cerebellar hemisphere	UBERON:0002245	62.70	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.32

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• a role for miR-128 in the molecular events modulating neuroblastoma progression and aggressiveness (PMID:19713529)
• Expression of miR-128b and miR-221 is down-regulated in MLL-rearranged ALL relative to other types of ALL. Reexpression of these miRNAs cooperatively sensitizes 2 cultured lines of MLL-AF4 ALL cells to glucocorticoids. (PMID:19749093)
• These results demonstrate that the novel mutation in miR-128b in MLL-AF4 acute lymphocytic leukemia alters the processing of miR-128b. (PMID:20237425)
• The transcriptional induction of miR-128-2 by mutant p53R175H results in the increased chemoresistance of cancer cells. (PMID:22193543)
• MiR-7 transfection into ovarian cancer cells induces changes in cell adhesion and other developmental networks, while miR-128 transfection induces changes in cell cycle control. (PMID:22853714)
• MiR-128-2 inhibits the expression of ABCA1, ABCG1 and RXRalpha directly through a miR-128-2-binding site within their respective 3’untranslated regions. (PMID:23990020)
• miR-128 inhibition in monocytes from AD patients improves Ab(1-42) degradation. These results could contribute to clarify the molecular mechanisms that affect the imbalanced Ab production/clearance involved in the pathogenesis of AD. (PMID:24064186)
• Data indicate that zinc finger protein SNAIL modulates ribosomal protein S6 kinase 1 (RPS6KB1)/HIF-1alpha/protein kinase 2 (PKM2) signaling pathway through miR-128. (PMID:24859886)
• Data revealed that upregulated SNAI1 accelerates glioma progression and suppresses the expression of miR-128, which can oppose SNAI1’s effect and modulate SP1 expression. (PMID:24959930)
• Study showed that the expression of miR-128 and miR-149 was downregulated in glioblastoma; the lower expression of miR-128 and miR-149 contributed to astrocytoma tumorigenesis. (PMID:25017996)
• Overexpression of miR-128b is associated with childhood acute lymphoblastic leukemia. (PMID:25388103)
• serum level of miR-128-2 serves as a noninvasive biomarker for the overall survival of patients with hepatocellular carcinoma (PMID:25642945)
• the expression of hsa-miR-128-3p is inversely correlated with the expression of CYP2C9 in hepatocellular carcinoma tumor tissues; the study helped to elucidate the mechanism of CYP2C9 regulation by hsa-miR-128-3p, and the inverse association in hepatocellular carcinoma (PMID:25704921)
• Data suggest that miR-128 acted as a tumor suppressor inhibiting the head and neck squamous cell carcinoma growth by directly mediating the expression of putative targets. (PMID:25764126)
• Decreased expression of miR-128 in both tissue and serum samples of prostate cancer patients may be associated with tumor malignant progression and BCR-free survival. (PMID:26339409)
• miR-27b, miR-101 and miR-128 inhibit angiogenesis by down-regulating VEGF-C expression in gastric cancers. (PMID:26460960)
• miR-128b is a proto-oncogene miRNA that can suppresses gastric tumor proliferation and migration through down-regulation of the oncogene gene A2bR. (PMID:26478435)
• n the conditon of miR- 128b over-expression, we also observed spontaneous inactivation of the Akt/NF-kappaB signalling, implying PDK1 was a potential regulator of this pathway. In conclusion, our study shed some novel light on miR-128b-PDK1/Akt/NF-kappaB axis onGastric cancer (GC) progression (PMID:26949090)
• miR-128 regulates the differentiation of human hair follicle mesenchymal stem cells into smooth muscle cells via targeting SMAD2, a main transcription regulator in TGF-beta signaling pathway involving smooth muscle cell differentiation. (PMID:27087048)
• AurkA suppresses the expression of miR-128, inhibitor of wnt3a mRNA stabilization. (PMID:27341528)
• Thus, VEGFA stimulates ovarian cancer stem-like cells through Src-DNMT3A-driven miR-128-2 methylation and Bmi1 upregulation. (PMID:28179359)
• Decreased miR-128 and increased miR-21 synergistically cause podocyte injury in sepsis. (PMID:28497421)
• Results reveal that miR-128 was downregulated in lung cancer tissues and induces lung cancer cell apoptosis by downregulating the expression of NEK2. (PMID:28514100)
• MiR-128-3p role in the non-small cell lung cancer drug resistance.MiR-128-3p induces epithelial-to-mesenchymal transition and cancer stem cells programming in the non-small cell lung cancer cells. (PMID:28627514)
• Low expression of miR-128 correlated with shorter overall and disease-free survival in triple-negative breast cancer (TNBC) (P < 0.001), and shorter overall but not disease-free survival in non-TNBC. In addition, miR-128 was able to inhibit glucose metabolism, mitochondrial respiration and proliferation of TNBC cells. (PMID:29116653)
• Through radiotherapy and chemotherapy assays, the function of miR-128a on chemoradiotherapy was evaluated. The correlation of miR-128a with BMI1 was identified by performing real-time PCR. (PMID:29186980)
• Our study provides mechanistic insights into the function of miR-128-3p as a key regulator of the malignant phenotype of lung cancer cells…and in particular it highlights a role for Drosha in non-small-cell lung cancer cells migration (PMID:29236960)
• miR-128 interacts with CCL18 3’UTR, reducing its expression in malignant melanoma. (PMID:30025750)
• PVT1-214 modulates the derepression of Lin28 by interacting with miR-128 (PMID:30076414)
• PVT1 can regulate VEGFC expression by competitively binding miR-128 in bladder cancer cells. (PMID:30076714)
• suggest that determination of miR-128 expression level may provide a tool for confirmation of a diagnosis of childhood (PMID:30139235)
• The recovery of miR-128 expression upregulated the expression of SIRT1, and thus promoted the TRAIL-induced generation of ROS in CRC cells. Although miR-128 could not upregulate DR5 directly, the combination of miR-128 with TRAIL was able to increase DR5 expression through the SIRT1/ROS/DR5 pathway in TRAIL-treated CRC cells. (PMID:30257253)
• Expression level of miR-128 in glioma tissues was significantly lower and NEK2 expression level was higher than that of normal brain tissues. MiR-128 expression was correlated to tumor size and malignant level of glioma, whereas not related to age and gender of glioma patients. Dual-luciferase reporter gene assay indicated that miR-128 directly bound to NEK2. (PMID:30575919)
• the PGC-1alpha/SNAI1/miR-128b axis plays a vital role in gastric cancer via regulating cell viability, migration, invasion, and apoptosis (PMID:30684287)
• The miR-128 functions through direct binding of L1 RNA and by regulating at least two cellular co-factors (hnRNPA1 and TNPO1), which L1 is dependent on for successful mobilization. (PMID:31010097)
• knockdown of HCP5 exerted anti-tumor effect via sponging miR-128-3p in Anaplastic thyroid cancer (PMID:31102936)
• The results revealed that miRNA128b regulated EGFR expression in in nonsmall cell lung cancer cells. (PMID:31638205)
• MiR-128-3p targets JAG1 to inhibit cell proliferation and invasion in melanoma. (PMID:32030966)
• Upregulated serum miR-128-3p in progressive and relapse-free multiple sclerosis patients. (PMID:32432792)
• miR-128 Regulates Tumor Cell CD47 Expression and Promotes Anti-tumor Immunity in Pancreatic Cancer. (PMID:32536914)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir128-2	ENSDARG00000083495

Paralogs (1): MIR128-1 (ENSG00000207654)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.