MIR133B

Summary

MIR133B (microRNA 133b, HGNC:31759) is a microRNA gene on chromosome 6p12.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The encoded miRNA is considered a canonical myomiR and is important for normal cardiac and skeletal muscle development. It is additionally important for normal development in non-muscle tissues, and is abnormally expressed in a variety of cancers. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442890 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31759
Approved symbol	MIR133B
Name	microRNA 133b
Location	6p12.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-133b
Ensembl gene	ENSG00000199080
Ensembl biotype	miRNA
OMIM	610946
Entrez	442890
RNAcentral	URS000075DAC1 — miRNA, 119 nt, 17 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362210

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362210 — 1 exons

Exon	Start	End
ENSE00001436973	52148923	52149041

Expression profiles

Bgee: expression breadth broad, 43 present calls, max score 91.32.

Top tissues by expression

43 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
skeletal muscle tissue	UBERON:0001134	91.32	gold quality
hindlimb stylopod muscle	UBERON:0004252	88.69	gold quality
muscle of leg	UBERON:0001383	84.44	gold quality
adrenal tissue	UBERON:0018303	84.42	gold quality
gastrocnemius	UBERON:0001388	82.88	gold quality
stomach	UBERON:0000945	81.93	gold quality
liver	UBERON:0002107	78.60	gold quality
prostate gland	UBERON:0002367	75.37	gold quality
kidney	UBERON:0002113	74.29	gold quality
colon	UBERON:0001155	73.00	gold quality
caudate nucleus	UBERON:0001873	72.86	gold quality
intestine	UBERON:0000160	72.54	gold quality
heart left ventricle	UBERON:0002084	71.68	gold quality
lower esophagus muscularis layer	UBERON:0035833	70.97	gold quality
left coronary artery	UBERON:0001626	70.03	gold quality
subcutaneous adipose tissue	UBERON:0002190	69.88	gold quality
ascending aorta	UBERON:0001496	69.19	gold quality
minor salivary gland	UBERON:0001830	69.11	gold quality
muscle layer of sigmoid colon	UBERON:0035805	68.37	gold quality
skin of abdomen	UBERON:0001416	67.57	gold quality
spleen	UBERON:0002106	67.43	gold quality
lung	UBERON:0002048	66.06	gold quality
blood	UBERON:0000178	65.44	gold quality
right ovary	UBERON:0002118	65.10	gold quality
transverse colon	UBERON:0001157	64.32	gold quality
tibial artery	UBERON:0007610	64.13	gold quality
vagina	UBERON:0000996	64.04	gold quality
skin of leg	UBERON:0001511	62.80	gold quality
substantia nigra	UBERON:0002038	62.50	gold quality
putamen	UBERON:0001874	62.38	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• role of miRNAs in mammalian midbrain dopaminergic neurons; study identified a miRNA, miR-133b, that is specifically expressed in midbrain dopaminergic neurons and is deficient in midbrain tissue from patients with Parkinson’s disease (PMID:17761882)
• over-expression of miR-133B increased apoptosis in response to gemcitabine and reduced MCL-1 and BCL2L2 expression. (PMID:19654003)
• miR-208 was upregulated, whereas miR-1 and miR-133a were downregulated in MI compared to healthy adult and fetal hearts. All four tested miRNAs were downregulated in fetal hearts compared to healthy adult hearts. (PMID:20029200)
• miR-133b showed dysregulation in myocardial infarction or fetal hearts when compared to healthy adults. (PMID:20075508)
• These results provide evidence that miR-133b regulated tumor cell proliferation and apoptosis through modulation of the hepatocyte growth factor receptor signaling pathway. (PMID:20505319)
• Downregulation of miR-133b is associated with esophageal squamous cell carcinoma. (PMID:21351259)
• We found that high expression of miR-185 and low expression of miR-133b were correlated with poor survival (p=0.001 and 0.028, respectively) and metastasis (p=0.007 and 0.036, respectively) in colorectal cancer. (PMID:21573504)
• The specific co-regulation of miR-133b and miR-206 with the Il17a/f locus shown in mice also extended to human Th17 cells. (PMID:21637854)
• Results describe the profile of miR-1, miR-133a, miR-133b and miR-206 in human muscle cells isolated during different stages of fetal development. (PMID:21645416)
• Upregulation of miR-133b shortens the latency of cervical carcinoma and activates the ERK and AKT1 signaling pathways. (PMID:22179829)
• Aberrant expression and functional significance ofthe miR-1/miR-133a and miR-206/miR-133b clusters in human cancers. (PMID:22308266)
• findings showed miR133a and miR-133b are expressed at in 2 hormone-insensitive prostate cancer cell lines, PC3 and DU145; ectopic expression of miR-133 inhibited cell proliferation, migration and invasion in these cells; first evidence that miR-133 may target EGFR (PMID:22407299)
• miR-133b influences major apoptosis pathways, suggesting an essential role for this molecule during cellular transformation, tumorigenesis and tissue homeostasis. (PMID:22532850)
• Studies indicate that the plasma miR-133 and miR-328 are increased 11- to 16-fold in patients with acute myocardial Infarction. (PMID:22713968)
• Data indicate that miR-133b can interact specifically with the 3’-UTR of epidermal growth factor receptor (EGFR) mRNA. (PMID:22883469)
• Our results suggest that downregulation of miR-133b and overexpression of fascin-1 may have an important role in the progression of gastrointestinal stromal tumor (PMID:23196799)
• MicroRNA-133 inhibits cell proliferation, migration and invasion by targeting epidermal growth factor receptor and its downstream effector proteins in bladder cancer. (PMID:23206218)
• Our data assessed specific miRNA profiling deregulation in OS clinical samples and suggest that the expression of miR-1 and miR-133b may control cell proliferation and cell cycle through MET protein expression modulation. (PMID:23229283)
• Downregulation of miR-133b is associated with gastric cancer. (PMID:23296701)
• Gli1 expression was positive in gastric cancer tissues and inversely correlated with miR-133b expression. (PMID:23936094)
• results show that downregulated miR-133b contributed to increased cell invasion and migration in CRC by negatively regulating CXCR4. (PMID:24330809)
• Over-expression of miR-133b in osteosarcoma cell lines inhibited cell proliferation, invasion and migration, and induced apoptosis. Further, over-expression of miR-133b decreased the expression of predicted target genes BCL2L2, MCL-1, IGF1R and MET. (PMID:24391788)
• MiR-133b plays an important role in gastric cancer metastasis. (PMID:24443799)
• TAp63 expression is downregulated in human colon cancer, leading to the transcriptional regulation of the expression of miR-133b. (PMID:24594999)
• miR-133b targeted and downregulated RB1CC1 in prostate cancer cells. (PMID:24610824)
• miR-145, miR-133a and miR-133b suppressed the proliferation, migration, invasion and cell cycle progression of gastric cancer cells through decreasing expression of Sp1 and its downstream proteins. (PMID:24613927)
• microRNA-133b downregulation and inhibition of cell proliferation, migration and invasion by targeting matrix metallopeptidase-9 in renal cell carcinoma. (PMID:24714873)
• It is not increased in dopamine neurons in Parkinson’s disease. (PMID:24742361)
• Results showed that miRNA 133b was lower in colorectal cancer tissue than that in adjacent tissue. After miR-133b transfection, its level was elevated and accompanied by lower proliferation in both SW-620 and HT-29 cells. (PMID:24870791)
• Circulating level of miR-133 is associated with acute myocardial infarction. (PMID:24885383)
• Findings suggest that the EVI-1 rs6774494 G > A single nucleotide polymorphism targeted by miRNA-206/133b may contribute to the pathogenesis of breast cancer. (PMID:24935473)
• miR133b is significantly reduced in recurrent prostate cancer specimens in comparison to non-recurrent PCa samples. (PMID:24967583)
• This review identified and summarized the results of studies of miR-133 with emphasis on its function in human diseases in muscle and cancer.[Review] (PMID:24975488)
• the aberrant expression of miR-133b and miR-206 may be implicated in tumorigenesis and tumor progression of osteosarcoma (PMID:25120799)
• results also suggest the involvement of miR-208a and miR-1 in the proliferation as well as anti-proliferative and anti-apoptotic roles of miR-133a/b. (PMID:25125495)
• Combined expression of miR-133b and miR-133a is an effective prognostic marker of esophageal squamous cell carcinoma malignancy. (PMID:25280517)
• miR-133b direct targeting hERG gene is downregulated in glioma. (PMID:25355491)
• miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in gastric cancer cells. (PMID:25433493)
• CTGF expression was negatively regulated by miR133b in the human colorectum, suggesting that miR133b and CTGF may be candidate therapeutic targets in colorectal cancer. (PMID:25501363)
• We have identified NUP214, a member of the massive nuclear pore complex, as a novel miR-133b target. (PMID:25743594)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-mir-133c	ENSDARG00000080081
danio_rerio	mir133b	ENSDARG00000083537
mus_musculus	Mir133b	ENSMUSG00000065480
rattus_norvegicus	Mir133b	ENSRNOG00000035571
drosophila_melanogaster	mir-133	FBGN0262445

Paralogs (2): MIR133A1 (ENSG00000283927), MIR133A2 (ENSG00000284508)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.