MIR135A2

Summary

MIR135A2 (microRNA 135a-2, HGNC:31521) is a microRNA gene on chromosome 12q23.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406926 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31521
Approved symbol	MIR135A2
Name	microRNA 135a-2
Location	12q23.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-135-2, hsa-mir-135a-2
Ensembl gene	ENSG00000207586
Ensembl biotype	miRNA
Entrez	406926
RNAcentral	URS00000A7BEB — miRNA, 100 nt, 87 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384854

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384854 — 1 exons

Exon	Start	End
ENSE00001499861	97563812	97563911

Expression profiles

Bgee: expression breadth tissue_specific, 8 present calls, max score 73.70.

Top tissues by expression

8 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of pancreas	UBERON:0001150	73.70	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	69.10	gold quality
left adrenal gland	UBERON:0001234	67.20	gold quality
prostate gland	UBERON:0002367	66.86	gold quality
tibial nerve	UBERON:0001323	62.99	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	60.05	gold quality
right frontal lobe	UBERON:0002810	49.96	gold quality
left testis	UBERON:0004533	37.65	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 34)

• These findings suggest an important role for miR-135a in renal fibrosis and inhibition of miR-135a might be an effective therapy for diabetic nephropathy. (PMID:24908566)
• The results demonstrate that miR-135a targets Bmi1 in pancreatic ductal adenocarcinoma and functions as a tumor suppressor. (PMID:25013381)
• A signature comprising three miRNAs (miR1290, miR196b, and miR135a*) enabled the prediction of a chemotherapeutic response (rather than progression-free and overall survival) with high accuracy in patients with recurring lung adenocarcinoma (PMID:25142144)
• miRNA-135a-5p increases BCL-2 via AP-2alpha and consequently enhances cell resistance to apoptosis. (PMID:25322930)
• Aberrant expression of Runx2 in aggressive tumor cells is related to the loss of specific Runx2-targeting miRNA135 and miRNA203. (PMID:25634212)
• miR-135a promotes cell proliferation in bladder cancer by targeting PHLPP2 and FOXO1, and is performed as an onco- (PMID:25888950)
• miR-135a and miR-135b were upregulated in models of podocyte injury and in glomeruli isolated from patients with focal segmental glomerulosclerosis. (PMID:26134897)
• Our findings suggested that miR-135a represents a potential onco-miRNA and plays an important role in malignant melanoma progression (PMID:26261511)
• TGF-beta1 down-regulated KLF4 by activating miR-135a-5p, promoting proliferation and metastasis in hepatocellular carcinoma (PMID:27302923)
• Serum miR-135a level was downregulated in NSCLC patients, and was associated with poor prognosis. (PMID:27525941)
• High intratumoral expression of miR-135a was associated with high risk of recurrence (HR = 4.2, p = 0.024, time to recurrence 8.8 +/- 2.0 vs. 24.8 +/- 4.4 months in patients with low miR-135a expression). (PMID:28100188)
• Hepatitis C virus promotes a STAT3 transcriptional programme in the liver of patients by suppressing its regulator PTPRD via upregulation of miR-135a-5p. (PMID:28159835)
• downregulation of miR-135a as a potential mechanism underlying the high NHE9 expression observed in subset of glioblastomas (PMID:29268774)
• Analysis localized LIMK2 SNP rs2073859 (G-to-A allele) within the microRNA-135a binding region and revealed lower LIMK2 G allele expression. The frequency of A genotypes was higher in the bladder cancer (BC) group than normal controls and correlated with risks of higher grade and stage BC. LIMK2 may function as an oncogene in human BC, while allele-specific regulation by microRNA-135a may influence disease risk. (PMID:30006972)
• Study showed that miR-135a expression was significantly reduced while FOXO1 was up-regulated in glioma tissues. miR-135a overexpression in U251 cells could prominently inhibit proliferation and invasion according to the transwell assays. Moreover, FOXO1 was recognized as the target for miR-135a and may partially reverse the functions of miR-135a in U251 cells. (PMID:30024610)
• MiR-135 is induced upon glutamine deprivation in pancreatic ductal adenocarcinoma cells.MiR-135 targets phosphofructokinase-1 and inhibits aerobic glycolysis. (PMID:30778058)
• This study revealed miR-135a-5p might be indicated as a perspective for lung cancer via targeting LOXL4. (PMID:30993701)
• miR-186 and miR-135a repressed ESR2 expression in granulosa cells (GCs), which further inhibited CDKN1A expression, promoted GC proliferation and repressed GC apoptosis. (PMID:31173821)
• GAS5 silencing repressed the malignant progression of atherosclerosis at least partly through upregulation of miR-135a. (PMID:31545249)
• Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis. (PMID:32222724)
• MicroRNA-135a-induced formation of CD133+ subpopulation with cancer stem cell properties in cervical cancer. (PMID:32415843)
• TRPC1 exacerbate metastasis in gastric cancer via ciRS-7/miR-135a-5p/TRPC1 axis. (PMID:32560824)
• miR135a5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer. (PMID:34036386)
• Hypoxia-Inducible Exosomes Facilitate Liver-Tropic Premetastatic Niche in Colorectal Cancer. (PMID:34110633)
• Expression of miR-135a-5p and its target gene JAK2 in spermatozoa of patients with asthenozoospermia. (PMID:34378214)
• Clinical value of miR-135 and miR-20a combined with multi-detector computed tomography in the diagnosis of gastric cancer. (PMID:34537058)
• ERp29 forms a feedback regulation loop with microRNA-135a-5p and promotes progression of colorectal cancer. (PMID:34667160)
• Single nucleotide polymorphisms in binding site of miRNA-135a and targeted gene IRS2 are correlated with multiple clinical features of PCOS: A study in Chinese women. (PMID:35124585)
• Mir 135a inhibits tumor cell proliferation by regulating the expression of notch pathway in hepatoblastoma. (PMID:35818205)
• Hsa-mir-135a Shows Potential as A Putative Diagnostic Biomarker in Saliva and Plasma for Endometriosis. (PMID:36009038)
• CircSTK39 suppresses the proliferation and invasion of bladder cancer by regulating the miR-135a-5p/NR3C2-mediated epithelial-mesenchymal transition signaling pathway. (PMID:36538242)
• Epigenetic and genetic inactivation of tumor suppressor miR-135a in non-small-cell lung cancer. (PMID:36869643)
• Placenta-derived exosomal miR-135a-5p promotes gestational diabetes mellitus pathogenesis by activating PI3K/AKT signalling pathway via SIRT1. (PMID:37667545)
• miR-135a Mediates Mitochondrial Oxidative Respiratory Function through SIRT1 to Regulate Atrial Fibrosis. (PMID:38228115)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-mir-135a	ENSDARG00000081047
mus_musculus	Mir135a-2	ENSMUSG00000065524

Paralogs (2): MIR135B (ENSG00000199059), MIR135A1 (ENSG00000207926)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.