MIR135B

Summary

MIR135B (microRNA 135b, HGNC:31760) is a microRNA gene on chromosome 1q32.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442891 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31760
Approved symbol	MIR135B
Name	microRNA 135b
Location	1q32.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-135b
Ensembl gene	ENSG00000199059
Ensembl biotype	miRNA
OMIM	619560
Entrez	442891
RNAcentral	URS0000759E75 — miRNA, 97 nt, 12 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362189

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362189 — 1 exons

Exon	Start	End
ENSE00001436952	205448302	205448398

Expression profiles

Bgee: expression breadth broad, 62 present calls, max score 87.10.

Top tissues by expression

62 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
olfactory segment of nasal mucosa	UBERON:0005386	87.10	gold quality
islet of Langerhans	UBERON:0000006	77.84	gold quality
blood	UBERON:0000178	76.50	gold quality
left uterine tube	UBERON:0001303	72.24	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	71.22	gold quality
body of pancreas	UBERON:0001150	70.75	gold quality
body of stomach	UBERON:0001161	70.39	gold quality
heart	UBERON:0000948	69.95	gold quality
right atrium auricular region	UBERON:0006631	69.84	gold quality
stomach	UBERON:0000945	69.64	gold quality
esophagus mucosa	UBERON:0002469	69.05	gold quality
esophagus	UBERON:0001043	69.01	gold quality
fallopian tube	UBERON:0003889	68.68	gold quality
lower esophagus muscularis layer	UBERON:0035833	68.57	gold quality
endometrium	UBERON:0001295	68.14	gold quality
lung	UBERON:0002048	67.76	gold quality
saliva-secreting gland	UBERON:0001044	67.69	gold quality
upper lobe of left lung	UBERON:0008952	67.27	gold quality
muscle layer of sigmoid colon	UBERON:0035805	67.18	gold quality
minor salivary gland	UBERON:0001830	67.06	gold quality
nucleus accumbens	UBERON:0001882	66.75	gold quality
right hemisphere of cerebellum	UBERON:0014890	66.63	gold quality
tonsil	UBERON:0002372	66.58	gold quality
right ovary	UBERON:0002118	66.56	gold quality
transverse colon	UBERON:0001157	66.48	gold quality
intestine	UBERON:0000160	66.18	gold quality
adult mammalian kidney	UBERON:0000082	65.91	gold quality
right lung	UBERON:0002167	65.77	gold quality
urinary bladder	UBERON:0001255	65.73	gold quality
left ovary	UBERON:0002119	65.73	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.21

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• This finding suggests that hsa-miR-135b may control osteoblastic differentiation of unrestricted somatic stem cells by regulating expression of bone-related genes. (PMID:19795981)
• Data show that microRNA-135b (miR-135b) mediates nucleophosmin-anaplastic lymphoma kinase (NPM-ALK)-driven oncogenicity and empowers IL-17-producing immunophenotype in anaplastic large cell lymphoma (ALCL). (PMID:22042699)
• miR-135b functions as an oncogene in colorectal cancer (PMID:22469014)
• Expression of miR-135b correlates with serum estradiol level and ER-beta mRNA expression in colorectal cancer patients’ cancer tissues. (PMID:23143558)
• providing an evidence against usage of serum miR-17-3p, miR-29a, miR-92a and miR-135b as new biomarkers for early detection of colorectal cancer. (PMID:23568010)
• Expression of miR-135b, LZTS1, LATS2 and nuclear TAZ predicts poor outcomes of non-small-cell lung cancer. (PMID:23695671)
• A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of lung cancer in Chinese by reducing CD133 expression. (PMID:23715500)
• miR135b has a role in restricting proliferative potential of normal human keratinocytes (PMID:24129066)
• Data indicate four predicted polymorphic miR-135b-5p target sites in the disrupted-in-schizophrenia-1 (DISC1) 3’UTR, and that miR-135b-5p regulates the level of DISC1 mRNA. (PMID:24169524)
• miR-135b is involved in the impaired osteogenic differentiation of MSCs derived from multiple myeloma patients (PMID:24223191)
• miR-135b downregulated MTSS1 expression and contributed to colorectal cancer cell invasion, indicating its involvement in colorectal cancer progression. (PMID:24343340)
• Galpha12gep oncogene inhibits FOXO1, which may result from the inhibition of FOXO1 de novo synthesis by miR-135b in conjunction with MDM2-mediated destabilization of FOXO1. (PMID:24631529)
• Stool-based miR-135b can be used as a noninvasive biomarker for the detection of olorectal cancer and advanced adenoma. (PMID:24691020)
• Authors identify miR-135b as a key downsteam effector of oncogenic pathways and a potential target for CRC treatment (PMID:24735923)
• PAX6 activates miR-135b to inhibit TGF-b and BMP signaling thereby differentiating hESC toward a neural phenotype. (PMID:24802670)
• The expression of miR-135b or GSK3beta was significantly association with ionizing radiation resistance of glioblastoma multiforme samples. (PMID:25265336)
• High miR-135b-5p expression levels are associated with nasopharyngeal carcinoma. (PMID:25292031)
• Data indicate that exosomal miR-135b directly suppressed its target factor-inhibiting hypoxia-inducible factor 1 (FIH-1) in endothelial cells. (PMID:25320245)
• miR-135b inhibitor significantly inhibited OS cells proliferation and invasion. Forced expression of FOXO1 showed the opposite effect, and FOXO1 knockdown abolished the effect of miR-135b inhibitor (PMID:25416447)
• Data show that heat shock transcription factor 1/miR-135b/reversion-inducing-cysteine-rich protein with kazal motifs and ecotropic viral integration site 5 axis provides novel insight into the mechanisms of hepatocellular carcinoma metastasis. (PMID:25537516)
• The results showed positive feedback loops between miR135b and PTEN inactivation in human colorectal cancers (PMID:25571954)
• Overexpression of miR-135b suppressed the invasion of extravillous trophoblast-derived HTR-8/SVneo cell by down regulating CXCL12. (PMID:25896762)
• demonstrates that miR-135b regulates ERalpha, AR and HIF1AN protein levels through interaction with their 3’UTR regions, and proliferation in ERalpha-positive BCa and AR-positive PCa cells (PMID:25907805)
• miR-135b may function as an oncogene by inhibiting GK5 in glioblastoma. (PMID:25936394)
• miR-135b expression inversely correlated with LZTS1 staining intensity and the Cutaneous Squamous Cell Carcinoma grade. (PMID:25938461)
• miR-135b exerted a tumor-promoting effect by inducing the proliferation and inhibiting the apoptosis of CRC cells via the negative regulation of TGFBR2 expression. (PMID:26061281)
• miR-135a and miR-135b were upregulated in models of podocyte injury and in glomeruli isolated from patients with focal segmental glomerulosclerosis. (PMID:26134897)
• We demonstrate that miR-135b and miR-146b target the CaSR and reduce its expression in colorectal tumors, reducing the antiproliferative and prodifferentiating actions of calcium. (PMID:26178670)
• MiR-135b-5p and MiR-499a-3p Promote Cell Proliferation and Migration in Atherosclerosis by Directly Targeting MEF2C (PMID:26184978)
• The expression levels of miR10b and miR135b are higher in semen with strong tissue specificity. (PMID:26355456)
• miR-135b promotes the invasion and metastasis possibly by targeting the Hippo pathway genes. (PMID:26429530)
• miR-135b down-regulated genes driving key pathways in glioblastoma cell proliferation, survival and migration/infiltration, suggesting possible tumor suppressor function. (PMID:26437223)
• Down-regulation of miR-135b evidently inhibited proliferation and arrested cell cycle in cervical cancer cells. Bioinformatics analysis predicted that the FOXO1 was a potential target gene of miR-135b. (PMID:26617737)
• Suggest that hsa-miR-29c and hsa-miR-135b are promising biomarkers of gastric carcinogenesis. (PMID:26877610)
• Findings demonstrated that miR-135b is upregulated in breast cancer tissues and cell lines, and is able to promote cellular proliferation, migration and invasion as well as disrupt the cell cycle in vitro via direct regulation of the expression of LATS2. (PMID:26934863)
• Data show the significance of circulating microRNAs miR-214 and miR-135b levels in detection of bone disease and in prediction of prognosis of patients with multiple myeloma, suggesting its potential clinical applications. (PMID:26995755)
• we report microRNA-135b (miR-135b), a key regulator of the malignancy, highly expressed in the RC component and promoting MLS cell invasion in vitro and metastasis in vivo through the direct suppression of thrombospondin 2 (THBS2). (PMID:27157622)
• The amplification of miR-135b suppressed non-small cell lung cancer chemoresistance by directly mediating FZD1 down-regulation. (PMID:27643554)
• Data show that miR-135b selectively targets the AMPK phosphatase Ppm1e. (PMID:27661114)
• Authors conclude that miR-135b-5p expression downregulates Ppm1e to activate AMPK signaling, which inhibits LPS-induced TNFalpha production via suppressing ROS production and NFkappaB activation. (PMID:27793001)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir135c-1	ENSDARG00000080100
danio_rerio	mir135c-2	ENSDARG00000080573
rattus_norvegicus	Mir135b	ENSRNOG00000035621

Paralogs (2): MIR135A2 (ENSG00000207586), MIR135A1 (ENSG00000207926)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.