MIR140

Summary

MIR140 (microRNA 140, HGNC:31527) is a microRNA gene on chromosome 16q22.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406932 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31527
Approved symbol	MIR140
Name	microRNA 140
Location	16q22.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-140, MIR-140
Ensembl gene	ENSG00000208017
Ensembl biotype	miRNA
OMIM	611894
Entrez	406932
RNAcentral	URS00005471BA — miRNA, 100 nt, 17 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385282

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385282 — 1 exons

Exon	Start	End
ENSE00001500288	69933081	69933180

Expression profiles

Bgee: expression breadth broad, 79 present calls, max score 88.78.

Top tissues by expression

79 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
metanephros cortex	UBERON:0010533	88.78	gold quality
sural nerve	UBERON:0015488	86.60	gold quality
kidney	UBERON:0002113	82.33	gold quality
adult mammalian kidney	UBERON:0000082	81.93	gold quality
blood	UBERON:0000178	77.82	gold quality
right lobe of liver	UBERON:0001114	76.87	gold quality
monocyte	CL:0000576	76.81	gold quality
liver	UBERON:0002107	76.69	gold quality
descending thoracic aorta	UBERON:0002345	75.36	gold quality
muscle of leg	UBERON:0001383	74.93	gold quality
left uterine tube	UBERON:0001303	73.94	gold quality
thoracic aorta	UBERON:0001515	73.93	gold quality
ascending aorta	UBERON:0001496	73.79	gold quality
gastrocnemius	UBERON:0001388	72.71	gold quality
right atrium auricular region	UBERON:0006631	72.19	gold quality
body of stomach	UBERON:0001161	71.97	gold quality
smooth muscle tissue	UBERON:0001135	71.85	gold quality
stomach	UBERON:0000945	71.57	gold quality
adrenal tissue	UBERON:0018303	71.36	gold quality
body of pancreas	UBERON:0001150	71.25	gold quality
right adrenal gland	UBERON:0001233	70.94	gold quality
heart	UBERON:0000948	70.90	gold quality
left coronary artery	UBERON:0001626	70.64	gold quality
urinary bladder	UBERON:0001255	70.63	gold quality
saliva-secreting gland	UBERON:0001044	70.50	gold quality
minor salivary gland	UBERON:0001830	69.93	gold quality
tibial artery	UBERON:0007610	69.89	gold quality
Brodmann (1909) area 9	UBERON:0013540	69.79	gold quality
heart left ventricle	UBERON:0002084	69.64	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	69.49	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.32

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ESR1, SOX9

Literature-anchored findings (GeneRIF, showing 40)

• The reduction in miR-140 expression in oateoarthritic cartilage and in response to IL-1beta may contribute to the abnormal gene expression pattern characteristic of osteoarthritis. (PMID:19714579)
• miR-140 may be a candidate target to develop novel therapeutic strategy to overcome drug resistance. (PMID:19734943)
• Regulation of the IGFBP-5 and MMP-13 genes by the microRNAs miR-140 and miR-27a in human osteoarthritic chondrocytes. (PMID:19948051)
• These results suggest that the single nucleotide polymorphism located in pre-miR-140 contributes to nonsyndromic cleft palate susceptibility by influencing the processing of miR-140. (PMID:20358594)
• Our data suggest that miR-140 has a large number of targets conserved between human and chicken and we validate one of these, BMP2. (PMID:21720209)
• these results provide new information about the roles of microRNA-22 and microRNA-140 in the regulation of NF-kappaB activity. (PMID:21798241)
• Infants With CA/AA genotypes at Rs7205289, located in the microRNA-140 gene, exposed to maternal passive smoking during the first trimester have increased risk of nonsyndromic cleft palate. (PMID:22012839)
• we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells (PMID:22052544)
• Expression of miRNA-140 and MMP-13 was induced by IL-1beta (PMID:22273691)
• While DDX20 normally suppresses NF-kappaB activity by regulating NF-kappaB-suppressing miRNA-140 function, this suppressive effect was lost in DDX20-deficient cells. (PMID:22445758)
• Data suggest that microRNA/mRNA pairs in hsa-miR-140-3p/RAD51AP1/, hsa-miR-145/E2F3, hsa-miR-139-5p/TOP2A, and hsa-miR-133a/GCLC were correlated with ovarian tumorigenesis. (PMID:22452920)
• L-Sox5 and Sox6 proteins enhance chondrogenic miR-140 microRNA expression by strengthening dimeric Sox9 activity (PMID:22547066)
• miR-140-3p modulates CD38 expression in human airway smooth muscle cells through direct binding to the CD38 untranslated region and indirect mechanisms involving activation of p38 MAPK and NF-kappaB. (PMID:22773691)
• acts as a liver tumor suppressor (PMID:22898998)
• Estrogen receptor alpha signaling regulates breast tumor-initiating cells by down-regulating miR-140 which targets the transcription factor SOX2 (PMID:23060440)
• miR-140-5p is down-regulated in hepatocellular carcinoma. miR-140-5p possesses the potency to suppress HCC growth and metastasis by regulating TGFBR1 and FGF9 (PMID:23401231)
• these results indicate a new role of miR-140 at early stages of endochondral ossification, opening a promising area of research in the field of skeletal development. (PMID:23529404)
• results support that a miR-140/ALDH1/SOX9 axis is critical to basal CSC self-renewal and tumor formation in vivo, suggesting that the miR-140 pathway may be a promising target for preventative strategies in patients with basal-like DCIS (PMID:23752191)
• Studies focused on the role of microRNAs in cartilage have identified miR-140 and -675 as playing important roles in regulation of cartilage homeostasis. (PMID:23754477)
• Data indicate that miR-140 downregulates insulin-like growth factor 1 receptor (IGF1R) by directly targeting its 3’ untranslated regions (3’ UTR). (PMID:24039995)
• microRNA-140 targets RALA and regulates chondrogenic differentiation of human mesenchymal stem cells by translational enhancement of SOX9 and ACAN. (PMID:24063364)
• This is the first study to provide evidence of new and differential roles for NFAT3 and SMAD3 in the osteoarthritis process in the regulation of miR-140 transcription (PMID:24257415)
• ADAM10 is not just a direct target of miR-140-5p, the repressed ADAM10 also helps to enhance the effect of miR-140-5p to other target genes: ERBB4 and PAX6. ERBB4 is “positively” regulated by miR-140-5p. (PMID:24530397)
• TNFalpha in the marrow microenvironment led to RANKL demethylation and re-expression in myeloma cells through DNMT1 repression and upregulation of miR-126-3p and miR-140, both known to repress DNMT1 translation. (PMID:24875904)
• miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSC (PMID:24928442)
• Together, our results highlight the significance of miR-140-5p/MMD axis in lung cancer, and miR-140-5p/MMD axis could serve as new molecular targets for the therapy against lung cancer. (PMID:24971538)
• Over-expression of miR-140-3p is correlated with recurrence and tumor invasion in spinal chordoma (PMID:25197358)
• MiR-140 expression was decreased in the EC tissues. (PMID:25322669)
• functional evidence for a novel FGFRL1 poly-miRTS rs4647940 in a previously known 4p16.3 locus, and experimental and clinical genetics studies have shown both FGFRL1 and hsa-miR-140-5p are important for bone formation. (PMID:25941324)
• Report inhibition of colorectal cancer stem cell survival and invasive potential by hsa-miR-140-5p mediated suppression of Smad2 and autophagy. (PMID:25980495)
• MIR-140-5p has an essential role in mesenchymal stem cell chondrogenesis. (PMID:26175215)
• miR-140 could inhibit the growth, migration, and metastasis of osteosarcoma cells (PMID:26219893)
• miR-140-5p acts as a tumor suppressor during ovarian carcinogenesis, inhibiting ovarian cancer growth partially by repressing PDGFRA expression. (PMID:26297547)
• Ionizing radiation promotes NRF2 nuclear translocation and subsequent activation of miR-140 transcription in human lung fibroblasts. (PMID:26300493)
• Studies indicate that expression of microRNA miR-140 is ubiquitous in chondrocyte cells during embryonic bone development. (PMID:26318829)
• Study showed downregulation of miR-140 in colorectal cancer (CRC) closely associated with overall survival. VEGFA, its target, is up-regulated in CRC cells suggesting a tumor suppressive role of miR-140-5p in CRC and progression by targeting VEGFA. (PMID:26402430)
• The increased level of intracellular ATP8A1 protein attenuated the inhibitor role of miR-140-3p in the growth and mobility of NSCLC cell. (PMID:26415732)
• demonstrated that reexpression of NEAT1 in miR-140 knockout adipocyte-derived stem cells is sufficient to restore their ability to undergo differentiation (PMID:26459763)
• Lower expression levels of miR-140-3p and miR-140-5p were detected in synovial tissue and synovial fibroblasts from patients with rheumatoid arthritis. (PMID:26473405)
• there was reciprocal repression between MALAT1 and miR-140, and miR-140 mediated the effects that MALAT1 knockdown exerted (PMID:26619802)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir140	ENSDARG00000080453
mus_musculus	Mir140	ENSMUSG00000065439
rattus_norvegicus	Mir140	ENSRNOG00000035609

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.