MIR146B

Summary

MIR146B (microRNA 146b, HGNC:32079) is a microRNA gene on chromosome 10q24.32.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574447 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32079
Approved symbol	MIR146B
Name	microRNA 146b
Location	10q24.32
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-146b
Ensembl gene	ENSG00000202569
Ensembl biotype	miRNA
OMIM	610567
Entrez	574447
RNAcentral	URS000075C2A0 — miRNA, 73 nt, 4 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000365699

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000365699 — 1 exons

Exon	Start	End
ENSE00001440462	102436512	102436584

Expression profiles

Bgee: expression breadth broad, 50 present calls, max score 85.97.

Top tissues by expression

50 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
myometrium	UBERON:0001296	85.97	gold quality
right atrium auricular region	UBERON:0006631	79.35	gold quality
blood	UBERON:0000178	76.16	gold quality
heart left ventricle	UBERON:0002084	75.06	gold quality
descending thoracic aorta	UBERON:0002345	74.45	gold quality
adenohypophysis	UBERON:0002196	74.37	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	73.84	gold quality
gall bladder	UBERON:0002110	73.45	gold quality
pituitary gland	UBERON:0000007	73.07	gold quality
monocyte	CL:0000576	72.44	gold quality
adrenal gland	UBERON:0002369	72.41	gold quality
left adrenal gland	UBERON:0001234	72.02	gold quality
left adrenal gland cortex	UBERON:0035825	71.82	gold quality
body of pancreas	UBERON:0001150	71.59	gold quality
liver	UBERON:0002107	70.16	gold quality
omental fat pad	UBERON:0010414	69.46	gold quality
lung	UBERON:0002048	69.39	gold quality
body of stomach	UBERON:0001161	69.33	gold quality
lower esophagus muscularis layer	UBERON:0035833	69.04	gold quality
skin of abdomen	UBERON:0001416	68.61	gold quality
esophagus mucosa	UBERON:0002469	68.52	gold quality
transverse colon	UBERON:0001157	68.46	gold quality
multicellular organism	UBERON:0000468	68.18	gold quality
right coronary artery	UBERON:0001625	67.47	gold quality
left coronary artery	UBERON:0001626	67.23	gold quality
ascending aorta	UBERON:0001496	67.22	gold quality
upper lobe of left lung	UBERON:0008952	67.14	gold quality
granulocyte	CL:0000094	66.68	gold quality
minor salivary gland	UBERON:0001830	66.14	gold quality
right adrenal gland cortex	UBERON:0035827	66.01	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.99

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• miR-146a/b as a negative regulator of constitutive NF-kappaB activity in a breast cancer setting and suggest that modulating miR-146a/b levels has therapeutic potential to suppress breast cancer metastases. (PMID:18504431)
• Results support the recent notion that modulating the levels of miR-146a or miR-146b could have a therapeutic potential to suppress breast cancer metastasis. (PMID:19190326)
• miR-146b is involved in glioma cell migration and invasion by targeting MMP16; implicating miR-146b as a metastasis-inhibiting miRNA in glioma (PMID:19265686)
• Based on cross-fold validation analyses, miR-146b alone was found to have the strongest prediction accuracy for stratifying prognostic groups at approximately 78%. (PMID:19584273)
• Our results show the potential importance of miR-221, miR-222, and miR-146b in determining the aggressive properties of papillary thyroid carcinoma (PMID:20406109)
• we report that miR-146b-5p suppresses expression of epidermal growth factor receptor (EGFR) in human glioblastoma cell lines (PMID:20874002)
• The authors report here the involvement of miR-146a and miR-146b-5p that bind to the same site in the 3’UTR of BRCA1 and down-regulate its expression as demonstrated using reporter assays. (PMID:21472990)
• Overexpression of miR-146b was associated with recurrence and distant metastases in thyroid carcinoma. (PMID:21537871)
• Only a minimal effect of pre-miR-146b overexpression on the malignant phenotype was seen in A549 cells. (PMID:21789255)
• Matrix metalloproteinase 16 (MMP16) was a downstream target of miR-146b-5p. (PMID:21823013)
• Our results confirm the oncogenic role of miR-146b-5p in thyroid follicular cells and contribute to knowledge regarding the modulation of TGF-beta signal transduction by miRNAs in PTCs. (PMID:21874046)
• miR-146b-5p decrease in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin (PMID:22393448)
• Expression profiles of miR-146b, miR-155, and miR-221 enables classification of thyroid cancer nodules, distinguishing benign from papillary thyroid carcinomas, starting from fine needle aspiration. (PMID:22728346)
• miR-146b is a novel prognostic factor of papillary thyroid carcinoma. (PMID:23264400)
• miR-146a and miR-146b-5p are expressed in human RPE cells in culture and their expression is highly induced by proinflammatory cytokines (IFN-gamma + TNF-alpha + IL-1beta). (PMID:23592910)
• miR-146a and miR-146b are induced in endothelial cells upon exposure to pro-inflammatory cytokines. (PMID:23733368)
• The overexpression of miR-146b-5p in glioblastoma cell lines led to MMP16 mRNA silencing, MMP2 inactivation, and the inhibition of tumour cell migration and invasion. (PMID:23796692)
• results thus identify miR-146b as an IL-10-responsive miR with an anti-inflammatory activity based on multiple targeting of components of the TLR4 pathway in monocytes (PMID:23798430)
• p16(INK4a) interacts with Sp1 through the fourth ankyrin repeat, which is crucial for Sp1 binding to the miR-141 and miR-146b-5p promoters and their transcriptional activation. (PMID:24163379)
• MicroRNA-222 and miR-146b were over-expressed 10.8-fold and 8.9-fold, respectively. (PMID:24301304)
• The results indicated that a novel role for miR-146b in adipose tissue inflammation and miR-146b may be an important mediator in the process of obesity complications via its own transcription mechanism. (PMID:24428800)
• higher expression of miR-146b was positively correlated with patient survival in breast cancer subtypes with increased IL6 expression and STAT3 phosphorylation. (PMID:24473196)
• Among the three isoforms of C/EBPbeta, C/EBPbeta LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. (PMID:24589738)
• miR-146b and miR-183 may influence follicular thyroid carcinoma development through the induction of migration and apoptosis inhibition. (PMID:24631480)
• The expression level of miRNA 146b was found to be increased in cases of thyroid cancer with well differentiated tumor with uncertain malignant potential (PMID:24643689)
• these results suggest that low expression of miR-146b-5p and miR-320d may be predictive of compromised responses of a subset of diffuse large B-cell lymphoma patients to treatment with the CHOP regimen (PMID:24931464)
• We conclude that miR-146b-5p has a role in cell proliferation and invasion, and that PRRX1 plays an important role in papillary thyroid carcinoma epithelial-mesenchymal transition and disease progression. (PMID:24946010)
• miR-146b, PDGFRA, and GATA-1 formed a regulatory circuit to promote erythroid and megakaryocytic differentiation. (PMID:24982425)
• MicroRNA-141 and microRNA-146b-5p have a role in inhibiting the prometastatic mesenchymal characteristics through the RNA-binding protein AUF1 targeting the transcription factor ZEB1 and the protein kinase AKT (PMID:25261470)
• miR-146b and miR-155 helped to discriminate between benign and malignant lesions (PMID:25456009)
• Up-regulation of miR-146a and miR-146b expression in tissues was related to carcinogenesis and deterioration of PTC. MicroRNA-146a and miR-146b expressed in thyroid tissue may act as potential biomarkers for PTC patients. (PMID:25524940)
• The present study proposed a miRNA signature for expression pattern in Epstein-Barr virus+ diffuse large B-cell lymphoma of the elderly and our findings suggest that hsa-miR-146b and hsa-miR-222 could be biomarkers and therapeutic targets. (PMID:25544772)
• MiR-146b-5p increased the cell surface levels of the Wnt receptors Frizzled-6 and LRP6 and enhanced Wnt/beta-catenin signaling by downregulating ZNRF3. (PMID:25547151)
• miR146b5p may be a potential therapeutic strategy for the treatment of cervical cancer through regulation of cell chemotaxis and the cell cycle. (PMID:25572123)
• miR-146b-5p may represent the molecular basis of remodeling in Atrial fibrillation acting as intracellular mediator in the maladaptive remodeling in atrial fibrosis in Atrial fibrillation. (PMID:25617731)
• Up-regulation of miR-155 and miR-146b decreases H. pylori (cagA+)-introduced IL6 overexpression, which might weaken the cleanup of H. pylori (cagA+) and contributes to ulcer. (PMID:25753878)
• the present study indicated that the mechanism of action of miR-146b-5p in GBC involves the regulation of EGFR expression. (PMID:25760482)
• Normal thyroid tissues and most benign goiters were negative for miR-146b-5p and miR-21; miR-146b-5p was mainly expressed in papillary carcinoma and was not expressed in most Follicular and anaplastic cancer. (PMID:25771986)
• results showed that papillary thyroid cancer cells overexpress miR-146b and miR-222 in exosomes (PMID:25819770)
• Ang-1 disrupts TLR4 signalling, resulting in inhibition of LPS-induced inflammatory responses in endothelial cells. This inhibition occurs through selective targeting of IRAK1 and TRAF6 proteins by miR-146b-5p. (PMID:25824148)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir146a	ENSDARG00000083310
mus_musculus	Mir146b	ENSMUSG00000070127
rattus_norvegicus	Mir146b	ENSRNOG00000040448

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.