MIR148A

Summary

MIR148A (microRNA 148a, HGNC:31535) is a microRNA gene on chromosome 7p15.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406940 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31535
Approved symbol	MIR148A
Name	microRNA 148a
Location	7p15.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-148, hsa-mir-148a
Ensembl gene	ENSG00000199085
Ensembl biotype	miRNA
OMIM	613786
Entrez	406940
RNAcentral	URS00004C7B32 — miRNA, 68 nt, 30 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362215

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362215 — 1 exons

Exon	Start	End
ENSE00001436978	25949919	25949986

Expression profiles

Bgee: expression breadth broad, 45 present calls, max score 88.58.

Top tissues by expression

45 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	88.58	gold quality
blood	UBERON:0000178	75.54	gold quality
body of pancreas	UBERON:0001150	73.59	gold quality
monocyte	CL:0000576	71.81	gold quality
heart left ventricle	UBERON:0002084	71.56	gold quality
stomach	UBERON:0000945	70.92	gold quality
body of stomach	UBERON:0001161	70.85	gold quality
fundus of stomach	UBERON:0001160	68.79	gold quality
endometrium	UBERON:0001295	68.16	gold quality
fallopian tube	UBERON:0003889	67.86	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.77	gold quality
right adrenal gland	UBERON:0001233	67.14	gold quality
esophagus mucosa	UBERON:0002469	66.48	gold quality
skin of leg	UBERON:0001511	66.38	gold quality
skin of abdomen	UBERON:0001416	65.84	gold quality
transverse colon	UBERON:0001157	65.63	gold quality
left adrenal gland	UBERON:0001234	65.26	gold quality
omental fat pad	UBERON:0010414	65.19	gold quality
lung	UBERON:0002048	65.08	gold quality
intestine	UBERON:0000160	64.77	gold quality
right lobe of thyroid gland	UBERON:0001119	64.58	gold quality
minor salivary gland	UBERON:0001830	63.75	gold quality
putamen	UBERON:0001874	63.43	gold quality
right atrium auricular region	UBERON:0006631	63.30	gold quality
left lobe of thyroid gland	UBERON:0001120	63.15	gold quality
spleen	UBERON:0002106	62.77	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	62.68	gold quality
upper lobe of left lung	UBERON:0008952	62.53	gold quality
muscle layer of sigmoid colon	UBERON:0035805	62.50	gold quality
small intestine Peyer’s patch	UBERON:0003454	62.39	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.51

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• MiR-148a attenuates paclitaxel resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression (PMID:20406806)
• Low miR-148a is associated with lymph recurrence in squamous cell esophageal carcinoma. (PMID:20628822)
• MiR-148 was shown to affect Mitf mRNA expression in melanoma cells through a conserved binding site in the 3’UTR sequence of mouse and human Mitf. (PMID:20644734)
• Potential contribution of miR-148a to the growth of human prostate cancer. (PMID:20820187)
• Underexpression of miR-148a and miR-20b in human embryonic stem cells (hESs) were differentiated toward Mesenchymal stem cell, compared with ESs, allows an increase in expression of the EPAS1. (PMID:21081659)
• Functional studies revealed that miR-138-2, miR-148a, and miR-488 repress (30%-60%) several candidate genes for panic disorder-GABRA6, CCKBR and POMC. (PMID:21168126)
• Data show that hypermethylation at miR-148a and miR-152 was associated with microsatellite-unstable tumors and hypermethylation at miR-18b with sporadic disease. (PMID:21327300)
• miR-148a suppresses the expression of Bcl-2 at the posttranscriptional level and induces apoptosis by activating a caspase cascade colorectal cancer (PMID:21455217)
• variation within the 3’UTR of HLA-C regulates binding of miR-148 to its target site, resulting in relatively low surface expression of alleles that bind this microRNA and high expression of HLA-C alleles that escape post-transcriptional regulation (PMID:21499264)
• frequently and dramatically downregulated in human advanced gastric cancer tissues (PMID:21552422)
• miR-152 and miR-148a may be involved in the carcinogenesis of ovarian cancer through deregulation of cell proliferation. (PMID:21971665)
• Results show an inverse correlation between miR-148a and ROCK1 expression in gastric cancer tissues. (PMID:21994419)
• DNMT1 was over-expressed in primary tumors and cell lines, while knockdown of DNMT1 using siRNA could decrease methylation level of miR-148a promoter and restore its expression (PMID:22167392)
• miR-148a is an important mediator of ACVR1, thus offering a new potential target for the development of therapeutic agents against Fibrodysplasia ossificans progressiva. (PMID:22408438)
• miR-148a and miR-152 down-regulate HLA-G expression by binding its 3’UTR (PMID:22438923)
• Up-regulated miR-148a in the liver was associated with the appearance of aggressive liver tumors. PTEN was suppressed by miR-148a. (PMID:22496917)
• we identified WNT10B as a direct target of miR-148a in cancer-associated fibroblasts from endometrial cancers (PMID:22890324)
• miR-148a and miR-152 act as tumor suppressors by targeting IGF-IR and IRS1, and that restoration of miR-148a/152 expression may provide a strategy for therapeutic application to treat BC patients. (PMID:22935141)
• MiR-148a status has a prognostic/predictive value in advanced CRC patients treated with conventional chemotherapy, which has important clinical implications in improving therapeutic strategies and personalized management of this malignancy. (PMID:23056401)
• beta-catenin in postnatal Osx-lineage cells critically regulates bone homeostasis by promoting osteoblast activity and suppressing osteoblast turnover, while restraining osteoclast and marrow fat formation. (PMID:23225151)
• miR-148a reduced the growth, epithelial-to-mesenchymal transition, invasion, and metastasis of HBx-expressing hepatocarcinoma cells through inhibition of HPIP-mediated mTOR signaling. (PMID:23321675)
• we demonstrate herein that miR-148a does not impact PDAC proliferation both in vitro and in vivo thus suggesting a weak potential as a therapeutic tool. (PMID:23383211)
• negative regulator of Myc, revealing heretofore unappreciated role in hepatocarcinogenesis (PMID:23389829)
• No correlation of hsa-miR-148a with expression of PXR or CYP3A4 in human livers from Chinese Han population. (PMID:23527115)
• miR-148a plays a pivotal role in the liver by promoting the hepatospecific phenotype and suppressing the invasiveness of transformed cells. (PMID:23532995)
• Results show that miR-148a may regulate RUNX3 expression through modulation of DNMT1-dependent DNA methylation in gastric cancer and highlight a miRNA-epigenetics regulation mechanism of gene expression. (PMID:23549984)
• Data indicate that knockdown of ROCK1 reversed epithelial-to-mesenchymal transition (EMT) resembling that of miR-148a overexpression. (PMID:23670799)
• overexpression of miRNA-148a significantly suppressed the migratory and invasive abilities of A549 and H1299 lung cancer cells. (PMID:23843100)
• The Wnt signal pathway was determined to be involved in the miR-148a-mediated inhibition of EMT. (PMID:23861222)
• Proteins associated with cancer progression neural development are regulated by miR-148a. (PMID:23869555)
• Data show that miR-148a downregulated vimentin expression and upregulated E-cadherin expression, suggesting that miR-148a inhibited epithelial-mesenchymal transition (EMT), and the SMAD2 gene was identified as the direct and functional target of miR-148a. (PMID:23873106)
• Human HTT gene expression regulation by Huntington’s disease variants of miR-137, miR-214, and miR-148a. (PMID:23965969)
• targeting CCKBR and Bcl-2, overexpression of miR-148a can suppress cell proliferation and induce apoptosis in human pancreatic cancer cell lines. (PMID:23975374)
• Results show that miR-148a may negatively regulate Met/Snail signaling and therefore inhibit the EMT and metastasis of hepatoma cells. These findings suggest miR-148a as an attractive candidate for cancer therapy. (PMID:24013226)
• MicroRNA-148a (miR-148a) which suppresses tumor growth by directly decreasing DNMT1 expression has been demonstrated as an important role for cancer therapy. The mechanisms for miR-148a in cancer will become potential future researches (PMID:24084367)
• High miR-148a expression is associated with medulloblastoma. (PMID:24203893)
• a MIR148A insertion/deletion polymorphism associates with its own expression levels, affecting the extent to which HLA-C is down-regulated, the level of HIV control, and the risk of Crohn disease (PMID:24248364)
• hsa-miR-148a promotes cartilage production and inhibits cartilage degradation by osteoarthritic chondrocytes. (PMID:24269634)
• MMP7 was found to be a direct and functional target of miR-148a. (PMID:24283384)
• MiR-148a reduced EGFR trafficking. (PMID:24425048)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-mir-152	ENSDARG00000080838
danio_rerio	dre-mir-148	ENSDARG00000083365

Paralogs (2): MIR148B (ENSG00000199122), MIR152 (ENSG00000207947)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.