MIR154

Summary

MIR154 (microRNA 154, HGNC:31541) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406946 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31541
Approved symbol	MIR154
Name	microRNA 154
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-154
Ensembl gene	ENSG00000207978
Ensembl biotype	miRNA
Entrez	406946
RNAcentral	URS00003AF18B — miRNA, 84 nt, 7 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385243

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385243 — 1 exons

Exon	Start	End
ENSE00001500249	101059755	101059838

Expression profiles

Bgee: expression breadth broad, 65 present calls, max score 95.95.

Top tissues by expression

65 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	95.95	gold quality
placenta	UBERON:0001987	81.81	gold quality
stomach	UBERON:0000945	80.14	gold quality
blood	UBERON:0000178	78.71	gold quality
gastrocnemius	UBERON:0001388	77.21	gold quality
left ovary	UBERON:0002119	75.77	gold quality
intestine	UBERON:0000160	75.04	gold quality
ovary	UBERON:0000992	74.96	gold quality
heart	UBERON:0000948	74.95	gold quality
kidney	UBERON:0002113	74.70	gold quality
lung	UBERON:0002048	74.55	gold quality
liver	UBERON:0002107	72.23	gold quality
tibial artery	UBERON:0007610	71.40	gold quality
heart left ventricle	UBERON:0002084	71.33	gold quality
right atrium auricular region	UBERON:0006631	70.81	gold quality
colon	UBERON:0001155	70.47	gold quality
body of stomach	UBERON:0001161	69.88	gold quality
endometrium	UBERON:0001295	69.45	gold quality
adipose tissue	UBERON:0001013	69.37	gold quality
skin of abdomen	UBERON:0001416	69.33	gold quality
omental fat pad	UBERON:0010414	69.31	gold quality
myometrium	UBERON:0001296	69.21	gold quality
small intestine Peyer’s patch	UBERON:0003454	68.76	gold quality
corpus callosum	UBERON:0002336	68.75	gold quality
prefrontal cortex	UBERON:0000451	67.99	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	67.58	gold quality
upper lobe of left lung	UBERON:0008952	67.52	gold quality
right hemisphere of cerebellum	UBERON:0014890	66.70	gold quality
minor salivary gland	UBERON:0001830	66.62	gold quality
left adrenal gland	UBERON:0001234	66.60	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.37

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 38)

• Expression of miR-154*, miR-376b, and miR-431* were suppressed in leukocytes from initial Grave disease patients. (PMID:22456620)
• Increases in mir-154 may allow the activation of fibrotic transcriptional programs in lung fibroblasts. (PMID:23043088)
• miR-154 plays a prominent role in prostate cancer proliferation by suppressing CCND2 (PMID:23428540)
• MiR-154 plays a role in regulating epithelial-mesenchymal transition by targeting HMGA2. (PMID:23591597)
• Mir-154, mapped to the 14q32.31 locus, regulates proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. (PMID:24166498)
• The toll-like receptor 2 is a direct target of miR-154 in colorectal cancer cells. (PMID:24242044)
• miR-154* and miR-379 play important roles in prostate cancer biology by facilitating tumor growth, EMT, and bone metastasis. (PMID:25324143)
• our results indicate that changes in serum miRNAs are associated with cigarette smoking and lung cancer and that let-7i-3p and miR-154-5p are potential biomarkers of smoking-related lung cancer. (PMID:25386559)
• miR-154-5p may play an important role as an inhibitor of proliferation, migration and invasion of prostate cancer by targeting E2F5 in prostate cancer cell lines (PMID:27074041)
• miR-154 was upregulated in CD133(+) Glioblastoma multiforme cell lines. (PMID:27338789)
• The results suggest that miR-154 downregulation may be involved in glioma formation and progression, and that miR-154 might serve as a potential prognostic biomarker for patients with this disease. (PMID:27417886)
• targeting of DKK2 by miR-154 leads to upregulation of beta-catenin expression and activation of the classical Wnt signaling pathway and cardiac fibroblasts. (PMID:27542661)
• Low miR154 expression is associated with Growth and Invasion of Gastric Cancer. (PMID:28800791)
• Results demonstrate that miR-154-5p is downregulated in glioblastoma and functions as a tumor suppressor, thus regulating glioblastoma cell growth, invasion and migration by targeting PIWIL1 in vitro and in vivo. Findings suggest that miR-154-5p may be a significant potential biomarker for prognosis evaluation and therapeutic procedure in malignant glioblastoma. (PMID:28842123)
• Data found that miR-154 was downregulated in bladder cancer (BC) tissues and lower miR-154 expression levels were strongly associated with worse overall survival rates of patients with BC. Further study suggested that this was due to decreased inhibition of BC cell proliferation, migration and invasion mediated by miR-154. (PMID:29048677)
• MiR-154 can inhibit GSK-3beta expression by activating Wnt/beta-catenin signaling pathway, which promotes myocardial fibrosis (PMID:29687862)
• miR-154 inhibits proliferation and induces apoptosis of human skin SCC cells by down-regulating WHSC1 and blocking the P53 signaling pathway (PMID:29727714)
• hsa-miR-154-5p, alone or in combination with hsa-miR-196b-5p, hsa-miR-378a-3p and hsa-miR-33a-5p and the clinical parameters of BMI and age, may be applicable for non-invasive diagnosis of the endrometriosis (PMID:29857988)
• High miR154 expression is associated with renal cell carcinoma. (PMID:30138594)
• the results of our studies illuminate how SNHG1 formed a regulatory network to confer an oncogenic function in colorectal cancer and suggest that SNHG1 may serve as a potential target for colorectal cancer diagnosis and treatment. (PMID:30266084)
• miR154 expression was significantly downregulated and negatively correlated with ATG7 expression in bladder cancer (BCa) tissues and cell lines. miR154 expression was associated with advanced T stage, lymphatic invasion, and distant metastasis. A xenograft study revealed that miR154 inhibited BCa cell growth in vivo and suppressed ATG7 expression. (PMID:30483807)
• Data show that 56% of Bcl2-associated athanogene 3 protein (BAG3+)/ dilated cardiomyopathy (DCM+) significantly co-expressed mir-154-5p and mir-182-5p, suggesting that co-expression of mir-154-5p and mir-182-5p may potentially show diagnostic value. (PMID:30792263)
• MiR-154 inhibits cells proliferation and metastasis in melanoma by targeting AURKA and serves as a novel prognostic indicator. (PMID:31173299)
• Oxidative injury in HUVECs was regulated by microRNA-154 targeting the Wnt/ss-catenin signaling pathway. (PMID:31359876)
• miR-154-3p and miR-487-3p synergistically modulate RHOA signaling in the carcinogenesis of thyroid cancer. (PMID:31820783)
• Circ_101064 regulates the proliferation, invasion and migration of glioma cells through miR-154-5p/ PIWIL1 axis. (PMID:31941603)
• Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes mellitus: a cross-sectional cohort study. (PMID:31950345)
• Upregulation of miRNA-154-5p prevents the tumorigenesis of osteosarcoma. (PMID:32000044)
• Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154. (PMID:32138544)
• Long non-coding RNA SNHG11 promotes cell proliferation, invasion and migration in glioma by targeting miR-154-5p. (PMID:32432753)
• Histone deacetylase 1 regulates the malignancy of oral cancer cells via miR-154-5p/PCNA axis. (PMID:32549181)
• Circular RNA circABCC4 acts as a ceRNA of miR-154-5p to improve cell viability, migration and invasion of breast cancer cells in vitro. (PMID:33023375)
• SP1 activated-lncRNA SNHG1 mediates the development of epilepsy via miR-154-5p/TLR5 axis. (PMID:33096314)
• lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation. (PMID:33195697)
• Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration. (PMID:34857012)
• Exosomes-derived miR-154-5p attenuates esophageal squamous cell carcinoma progression and angiogenesis by targeting kinesin family member 14. (PMID:35156510)
• LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cell-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axis. (PMID:37326687)
• MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo. (PMID:37397007)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir154	ENSMUSG00000065448
rattus_norvegicus	Mir154	ENSRNOG00000035600

Paralogs (16): MIR494 (ENSG00000194717), MIR377 (ENSG00000199015), MIR381 (ENSG00000199020), MIR369 (ENSG00000199025), MIR323A (ENSG00000199069), MIR410 (ENSG00000199092), MIR409 (ENSG00000199107), MIR539 (ENSG00000202560), MIR487A (ENSG00000207742), MIR487B (ENSG00000207754), MIR656 (ENSG00000207959), MIR496 (ENSG00000207961), MIR323B (ENSG00000208004), MIR1185-1 (ENSG00000221525), MIR1185-2 (ENSG00000221614), MIR382 (ENSG00000283170)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.