MIR15B

Summary

MIR15B (microRNA 15b, HGNC:31544) is a microRNA gene on chromosome 3q25.33.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406949 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31544
Approved symbol	MIR15B
Name	microRNA 15b
Location	3q25.33
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-15b
Ensembl gene	ENSG00000207779
Ensembl biotype	miRNA
OMIM	619572
Entrez	406949
RNAcentral	URS000041CB9C — miRNA, 98 nt, 8 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385045

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385045 — 1 exons

Exon	Start	End
ENSE00001500052	160404588	160404685

Expression profiles

Bgee: expression breadth broad, 46 present calls, max score 99.46.

Top tissues by expression

46 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	99.46	gold quality
sural nerve	UBERON:0015488	96.58	gold quality
endometrium	UBERON:0001295	87.16	gold quality
uterus	UBERON:0000995	87.12	gold quality
kidney	UBERON:0002113	80.73	gold quality
blood	UBERON:0000178	80.05	gold quality
heart	UBERON:0000948	77.50	gold quality
gastrocnemius	UBERON:0001388	76.77	gold quality
lung	UBERON:0002048	76.37	gold quality
intestine	UBERON:0000160	72.26	gold quality
stomach	UBERON:0000945	71.17	gold quality
corpus callosum	UBERON:0002336	70.61	gold quality
body of stomach	UBERON:0001161	70.07	gold quality
muscle layer of sigmoid colon	UBERON:0035805	69.74	gold quality
esophagus mucosa	UBERON:0002469	68.88	gold quality
ascending aorta	UBERON:0001496	68.82	gold quality
granulocyte	CL:0000094	68.18	gold quality
tibial artery	UBERON:0007610	67.91	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.84	gold quality
colon	UBERON:0001155	67.62	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	67.61	gold quality
transverse colon	UBERON:0001157	67.49	gold quality
spleen	UBERON:0002106	66.79	gold quality
omental fat pad	UBERON:0010414	66.31	gold quality
putamen	UBERON:0001874	66.13	gold quality
right frontal lobe	UBERON:0002810	65.74	gold quality
skin of abdomen	UBERON:0001416	65.62	gold quality
right atrium auricular region	UBERON:0006631	65.47	gold quality
vagina	UBERON:0000996	65.46	gold quality
skin of leg	UBERON:0001511	64.10	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.32

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• These findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules. (PMID:19135980)
• miR-15b expression in hepatocellular carcinoma tissues may have a role in prventing recurrence (PMID:19956871)
• data suggest that reduced expression of miR-200b and miR-15b underscores the mechanisms of chemotherapy-induced epithelial-mesenchymal transition in tongue squamous cell carcinoma (PMID:21725369)
• The expression of miR-15b was shown to be highly correlated with that of five selected E2F-induced genes. (PMID:22045185)
• miR-106b and miR-15b maybe mediated as robust regulators in apoptosis or angiogenesis following MI, respectively (PMID:22613985)
• increased serum miR-15b level is a potentially biomarker for the diagnosis of fatty liver disease. (PMID:23287814)
• Overexpression of microRNA-15b is associated with the reduction of VEGFR-2 expression and cellular migration and tubulogenesis. (PMID:23688497)
• Data indicate that treatment of gastric cancer cells with dihydroartemisinin (DHA) increased miR-15b and miR-16 expression, caused a downregulation of Bcl-2, resulting in apoptosis of gastric cancer cells. (PMID:23907579)
• MicroRNA-15b (miR-15b) was downregulated in patients with HCC. (PMID:24122375)
• The miR-15b is induced by PDGF in pulmonary artery smooth muscle cells and is critical for PDGF-mediated repression of SMC-specific genes. (PMID:24152911)
• miR-15b can act as a positive regulator for osteoblast differentiation. (PMID:24435757)
• miR-15b may function as a glioma suppressor by targeting the Cyclin D1, which may provide a novel therapeutic strategy for treatment of glioma (PMID:24995320)
• DNA damage by irradiation induces miR-15b expression, causing up-regulation of ATM/Chk1. (PMID:25092292)
• Identify the miR-15 family as a novel regulator of cardiac hypertrophy and fibrosis acting by inhibition of the TGFbeta-pathway. (PMID:25103110)
• Rsults show that serum miR-15b levels significantly increased over time in recurrent melanoma patients comparing to nonrecurrent patients. (PMID:25155861)
• study demonstrated three miRNAs, including miR-15b, -27a, and -233 have a good clinical value in EEC diagnosis. (PMID:25329674)
• This study showed that the mir15b up regulation in patient with bipolar disorders. (PMID:25708817)
• Increased miR-15b expression in lung adenocarcinoma patients treated with cisplatin-based chemotherapy was correlated with low expression of PEBP4, decreased sensitivity to cisplatin and poor prognosis. (PMID:25721211)
• EGF induces microRNAs that target suppressors of cell migration: miR-15b targets MTSS1 in breast cancer. (PMID:25783158)
• This study demonstrated that miR-15b play a role in the inhibition of insulin resistance via reduced TNFalpha and SOCS3 signaling and increased IGFBP-3 levels, resulting in REC protection from hyperglycemia-induced apoptosis. (PMID:25888955)
• mangiferin regulates proliferation and apoptosis in glioma cells by induction of miR-15b and inhibition of MMP-9 expression (PMID:25901555)
• miR-15b might be involved in termination of osteoblastic cells proliferation by arresting them at G0/G1 phase through direct targeting cyclin E1. (PMID:26007664)
• Underexpression of miR-15b-5p is associated with hepatocellular carcinoma. (PMID:26023735)
• miR-15b is downregulated in Myasthenia Gravis patients and directly regulates the expression of Interleukin-15 in experimental Myasthenia Gravis mice. (PMID:26087886)
• Circulating miR-15b-5p, miR-338-5p, and miR-764 may be biomarkers for diagnosis of HCC (PMID:26119771)
• the activation of TLR7 increased CCND3 expression via the downregulation of miR-15b in B cells. (PMID:26144250)
• Data show that miR15b mediates SMURF2 expression in pancreatic cancer and suggest miR15b as an oncogene by promoting epithelial-mesenchymal transition and SMURF2 as a tumor suppressor gene which expression is inhibited by miR15b in pancreatic cancer. (PMID:26166038)
• Suggest that miR-15b may be a prognostic predictor and be involved in malignant progression of glioma. (PMID:26191187)
• Results indicate that the nucleotide sequence in the 3’ untranslated region (3’ UTR) of ring finger protein 125 (RNF125) is a potential microRNA miR-15b targeting site. (PMID:26202983)
• miR-15b/16-2 loss has an important role in the pathogenesis of B-cell chronic lymphocytic leukemia (PMID:26324892)
• mechanisms involved in regulating miR-15b expression in mammalian tumors and discuss the effects of miR-15b dysregulation on the hallmarks of cancer [review] (PMID:26586400)
• MiR-15b was greatly upregulated in aMCL. (PMID:26676972)
• High abundance of circulating miR-15b correlated with tumor metastasis. (PMID:26743779)
• MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression. (PMID:26884837)
• observations indicate that induced expression of miR-15b is modulated by c-Rel and CREB in response to JEV infection (PMID:26931521)
• negative regulator of stress-induced SIRT4 expression, thereby counteracting senescence associated mitochondrial dysfunction and regulating the senescence-associated secretory phenotype and possibly organ aging, such as photoaging of human skin. (PMID:26959556)
• WEE1 is regulated at the translational level by CPEB1 and miR-15b in a coordinated and cell-cycle-dependent manner. (PMID:27027998)
• the miR-15b expression is negatively associated with the cripto-1 expression in glioma cells. miR-15b may subsequently impair growth and invasion of glioma cells through targeted regulation of cripto-1. (PMID:27082313)
• Mir15b directly regulates WNT7A expression in ovarian cancer cell line. (PMID:27195958)
• We were able to demonstrate that the expression levels of three microRNAs (miR-10b, -15b and -139) in the supernatants of four breast cancer cell lines were specifically and significantly down-regulated following hyperthermia exposure. (PMID:27380148)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir15c	ENSDARG00000081366
mus_musculus	Mir15b	ENSMUSG00000065580
rattus_norvegicus	Mir15b	ENSRNOG00000035483

Paralogs (3): MIR16-2 (ENSG00000198987), MIR15A (ENSG00000283785), MIR195 (ENSG00000284112)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.