MIR185

Summary

MIR185 (microRNA 185, HGNC:31556) is a microRNA gene on chromosome 22q11.21.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406961 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31556
Approved symbol	MIR185
Name	microRNA 185
Location	22q11.21
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-185
Ensembl gene	ENSG00000208023
Ensembl biotype	miRNA
OMIM	615576
Entrez	406961
RNAcentral	URS000075D261 — miRNA, 82 nt, 9 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385288

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385288 — 1 exons

Exon	Start	End
ENSE00001500294	20033139	20033220

Expression profiles

Bgee: expression breadth broad, 66 present calls, max score 90.39.

Top tissues by expression

66 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	90.39	gold quality
blood	UBERON:0000178	79.38	gold quality
adrenal tissue	UBERON:0018303	79.18	gold quality
monocyte	CL:0000576	74.84	gold quality
gastrocnemius	UBERON:0001388	74.28	gold quality
bone marrow	UBERON:0002371	74.09	gold quality
tibial nerve	UBERON:0001323	72.47	gold quality
endometrium	UBERON:0001295	71.62	gold quality
adult mammalian kidney	UBERON:0000082	71.19	gold quality
heart	UBERON:0000948	70.89	gold quality
left adrenal gland cortex	UBERON:0035825	70.84	gold quality
stomach	UBERON:0000945	70.58	gold quality
heart left ventricle	UBERON:0002084	70.39	gold quality
body of stomach	UBERON:0001161	69.69	gold quality
liver	UBERON:0002107	68.44	gold quality
tibial artery	UBERON:0007610	68.37	gold quality
anterior cingulate cortex	UBERON:0009835	68.18	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	67.50	gold quality
right atrium auricular region	UBERON:0006631	67.36	gold quality
thoracic mammary gland	UBERON:0005200	67.31	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.22	gold quality
left adrenal gland	UBERON:0001234	67.09	gold quality
body of pancreas	UBERON:0001150	66.83	gold quality
ascending aorta	UBERON:0001496	66.54	gold quality
transverse colon	UBERON:0001157	66.04	gold quality
skin of abdomen	UBERON:0001416	65.39	gold quality
omental fat pad	UBERON:0010414	65.22	gold quality
lung	UBERON:0002048	65.15	gold quality
left ovary	UBERON:0002119	64.97	gold quality
putamen	UBERON:0001874	64.94	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.08

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): SREBF1

Literature-anchored findings (GeneRIF, showing 40)

• We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers (PMID:19688090)
• Altered expression of the novel tumor suppressor miR-185 may be one of the central events that leads to dysregulation of oncogenic protein Six1 in human cancers. (PMID:20603620)
• Data show that miR-185 is a negative regulator of RhoA and Cdc42 and their cellular activities, and could inhibit proliferation and invasion of colorectal cancer cells. (PMID:21186079)
• We found that high expression of miR-185 and low expression of miR-133b were correlated with poor survival (p=0.001 and 0.028, respectively) and metastasis (p=0.007 and 0.036, respectively) in colorectal cancer. (PMID:21573504)
• unveil miR-185-3p as the first miRNA that monitors c-Myc levels via an autoregulatory feedback mechanism in response to serum stimulation. (PMID:21832077)
• MiR-185 targets the DNA methyltransferases 1 and regulates global DNA methylation in human glioma (PMID:21962230)
• miRNA-185 plays a role in the growth and invasion of glioma cells (PMID:22834685)
• Results indicate that miR-152 and miR-185 were involved in ovarian cancer cisplatin resistance in vitro and in vivo by targeting DNMT1 directly. (PMID:23318422)
• miR-185 could function as a tumor-suppressor gene in prostate cancer by directly targeting androgen receptor. (PMID:23417242)
• Data indicate that, in 22q11.2 deletion/DiGeorge syndrome, eighteen microRNAs had a statistically significant differential expression with miR-185 expressed at 0.4x normal levels. (PMID:23454892)
• miR-185, miR-96, and miR-223 may repress selective HDL-cholesterol uptake through the inhibition of SR-BI in human hepatic cells. (PMID:23459944)
• our study demonstrated that the low expression level of miR-185 led to a lower survival rate and more recurrence of hepatocellular carcinoma (HCC) in early stage, and miR-185 could suppress the HCC cell growth and invasive ability in vitro (PMID:23648054)
• A novel regulatory mechanism for ATR gene expression mediated by miR-185. (PMID:23807228)
• Our results show that GKN1 has an miR-185-dependent and -independent mechanism for chromatic and DNA epigenetic modification, thereby regulating the cell cycle (PMID:23846337)
• miR-185 plays a role in up-regulation of GPX2 and SEPHS2 expression. (PMID:23934683)
• miR-185 and 342 control lipogenesis and cholesterogenesis in prostate cancer cells by inhibiting SREBP-1 and 2 expression and downregulating their targeted genes, FASN and HMGCR. (PMID:23951060)
• miR-185 regulates T cell development through its targeting of several mRNAs including Mzb1. (PMID:24014023)
• a novel axis regulating cholesterol homeostasis exists that exploits miR-185-dependent regulation of SREBP-2 and requires SREBP-1c for function (PMID:24296663)
• findings demonstrate that the miR-185 is important for gastric cancer initiation and progression and holds promise as a prognostic biomarker to predict survival and relapse in gastric cancer (PMID:24352663)
• RUNX3, miR-185 and ARC regulate the sensitivity of gastric cancer cells to chemotherapy. (PMID:24763054)
• miR-185 directly targeted to EphB2 mRNA and suppressed its expression. miR-185 overexpression inhibited B-cell activation. (PMID:24803541)
• miR-185 inhibits Hepatocellular carcinoma cell growth by targeting DNMT1, leading to PTEN induction and Akt inhibition. (PMID:24911372)
• Human genetic analyses generated no evidence of the involvement of MIR185 in schizophrenia (PMID:24936775)
• miR185 may be a tumor suppressor, affecting the development of colon cancer via inhibition of HIF2a signaling. (PMID:25216407)
• high levels of miRNA-185 in RCC are associated with high VEGFR-2 mRNA and protein levels and a higher density of microvessels. (PMID:25217984)
• Results showed that miR-185-3p contributs to radioresistance of nasopharyngeal carcinoma via modulation of WNT2B expression. (PMID:25297925)
• miR-185 suppresses tumor proliferation by directly targeting E2F6 and DNMT1 and indirectly upregulating BRCA1 in triple-negative breast cancer. (PMID:25319390)
• miR-185-mediated Vegfa targeting may be involved in breast cancer formation (PMID:25448984)
• Expression of STIM1 is regulated by a posttranscriptional regulatory mechanism mediated by a new Epithelial-to-mesenchymal transition-related miRNA mir-185. (PMID:25531324)
• MiR-185 plays an important role in the regulation of insulin secretion and beta-cell growth in diabetes. (PMID:25658748)
• MiR185 directly targeted the AR3’UTR, to inhibit the expression of AR, and acted as a tumor suppressor in the PCa cells. (PMID:25673182)
• Our results suggest that the miR-185, as a tumor suppressor, plays a pivotal role by inhibiting VEGFA in von Hippel-Lindau gene-inactivated clear cell renal cell carcinomas (PMID:25700976)
• HCV core protein disturbs the cholesterol homeostasis in HepG2 cells via the SREBP2 pathway; miR-185-5p is involved in the regulation of SREBP2 by the core protein. (PMID:25914460)
• mir-185 regulates the expression of SSTR2 in GH-secreting pituitary adenoma. (PMID:26036598)
• The miR-185 was present with quite low abundance in acute phase ischemic stroke compared with healthy individuals. (PMID:26044809)
• Results suggest that TRIM29 functions as an oncogene in gastric cancer and is regulated by miR-185. (PMID:26191199)
• Identify RAGE as a target of miR-185 in esophageal squamous cell carcinoma. (PMID:26316588)
• assess the clinical values of low miR-185-3p and low miR-324-3p expression in predicting response to radiotherapy (RT) and prognosis of NPC (PMID:26390174)
• These findings suggest that plasma miR-185 has become potential biomarkers for glioma and may be useful in clinical management for glioma patients. (PMID:26458593)
• miR-185 controls cholesterol homeostasis as a key posttranscriptional LDLR modulator in hepatic cells, providing novel insight into the regulatory mechanism for LDLR expression and the anti-atherosclerosis effect of miR-185-inhibitor. (PMID:26523989)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir185	ENSMUSG00000065464
rattus_norvegicus	Mir185	ENSRNOG00000035585

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.