MIR196B

Summary

MIR196B (microRNA 196b, HGNC:31790) is a microRNA gene on chromosome 7p15.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442920 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31790
Approved symbol	MIR196B
Name	microRNA 196b
Location	7p15.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-196b
Ensembl gene	ENSG00000283745
Ensembl biotype	miRNA
OMIM	609688
Entrez	442920
RNAcentral	URS000075D019 — miRNA, 84 nt, 20 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384852

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384852 — 1 exons

Exon	Start	End
ENSE00001499859	27169480	27169563

Expression profiles

Bgee: expression breadth broad, 18 present calls, max score 74.85.

Top tissues by expression

18 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
endometrium	UBERON:0001295	74.85	gold quality
blood	UBERON:0000178	67.83	gold quality
left adrenal gland	UBERON:0001234	67.11	gold quality
renal glomerulus	UBERON:0000074	65.09	gold quality
gastrocnemius	UBERON:0001388	64.24	gold quality
lower esophagus muscularis layer	UBERON:0035833	64.22	gold quality
uterus	UBERON:0000995	61.82	gold quality
left lobe of thyroid gland	UBERON:0001120	60.21	gold quality
small intestine Peyer’s patch	UBERON:0003454	57.75	gold quality
prostate gland	UBERON:0002367	57.08	gold quality
skin of leg	UBERON:0001511	56.15	gold quality
subcutaneous adipose tissue	UBERON:0002190	56.09	gold quality
pituitary gland	UBERON:0000007	55.71	gold quality
tibial nerve	UBERON:0001323	53.74	gold quality
muscle layer of sigmoid colon	UBERON:0035805	53.00	gold quality
body of uterus	UBERON:0009853	51.85	gold quality
skin of abdomen	UBERON:0001416	51.54	gold quality
transverse colon	UBERON:0001157	48.57	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ETS2

Literature-anchored findings (GeneRIF, showing 40)

• identified miR-196b as a candidate microRNA that could contribute to SNP-specific expression of HMGA2 during human prenatal development. (PMID:20058197)
• High expression of miR-196b is not exclusively MLL-driven but can also be found in other types of leukemia with aberrant activation of HOXA genes. (PMID:20494936)
• Suggest that miR-196b functions as a tumor suppressor in B-cell lineage acute lymphoblastic leukemia. (PMID:20549547)
• our results identify miR-196a and miR-196b as ERG regulators and implicate a potential role for these miRNAs in acute leukemia. (PMID:20570349)
• results provide important information on miR-196s regulation and demonstrate that abnormal DNA hypomethylation induces overexpression of miR-196b in gastric cance (PMID:20662076)
• Results of the present study revealed that miR-196b becomes non-functional in T-cell ALL as a consequence of mutations in 3’-UTR of c-myc gene in T-cell ALL cellular models (PMID:20924650)
• The study demonstrates that HCV RNA replication in PBMCs of CHC patients is connected with the increased and coordinated expression of miRNA-155 and miRNA-196b. (PMID:21750860)
• Higher expression levels of miR-196b is associated with precursor T-Cell Lymphoblastic Leukemia-Lymphoma. (PMID:22099053)
• demonstrated that miR-196b was transcriptionally regulated by ETS2 and there was an inverse expression profile between miR-196b and ETS2 in clinical samples (PMID:22298639)
• findings show that HOXA9 and MEIS1 are direct targets of miRNA-196b, a microRNA located adjacent to and co-expressed with HOXA9, in MLL-rearranged leukaemic cells (PMID:22353710)
• MiR-196b is overexpressed and confers a poor prognosis via promoting cellular proliferation in GBM patients. (PMID:22723849)
• we found that both miR-10b and miR-196b show elevated expression in human high-grade breast tumor vasculature (PMID:22836757)
• miR-196b expression is significantly upregulated in hepatocellular carcinoma compared to non-tumor tissue. (PMID:22976466)
• Data have provided the first evidence that expression of miR-196b was associated with the occurrence of pre-operative seizures in low-grade gliomas, and may predict seizure prognosis in patients without pre-operative seizures. (PMID:23049982)
• Data suggest that expression of MIR196B in stromal cells from endometriotic ovary is repressed by DNA hypermethylation of MIR196B gene; this repression may be involved in development of proliferative/anti-apoptotic characteristics of endometriosis. (PMID:23293219)
• MicroRNA-196b regulates the HOXB7-VEGF axis in cervical cancer. (PMID:23861821)
• A low level of expression of miR-196b can cause up-regulation of BCR-ABL1 and HOXA9 expression, which leads to the development of chronic myeloid leukemia. (PMID:23894305)
• Co-activation of miR-196b and HOXA10 characterized a poor-prognosis subgroup of patients with gastric cancer. (PMID:24222951)
• Inhibition of microRNA-21 and microRNA-196b inhibited in vitro leukemic colony forming activity and depleted in vivo leukemia-initiating cell activity. (PMID:24334453)
• miR-196b inhibits hepatis C virus replication. (PMID:24591085)
• A signature comprising three miRNAs (miR1290, miR196b, and miR135a*) enabled the prediction of a chemotherapeutic response (rather than progression-free and overall survival) with high accuracy in patients with recurring lung adenocarcinoma (PMID:25142144)
• the mi-RNAs hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p were induced in human umbilical vein endothelial cells following expression of EBOV GP (PMID:25218824)
• miR-196 performed it’s their function by inhibiting NME4 expression and further activating p-JNK, suppressing TIMP1, and augmenting MMP1/9. (PMID:25233933)
• miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation. (PMID:25475721)
• Combined determination of circulating miR-196a and miR-196b levels may serve as panel plasma biomarkers for the early detection of oral cancer. (PMID:25485932)
• Results suggest that the up-regulation of MIR196B modulates apoptosis in colorectal cancer cells by partially repressing FAS expression. (PMID:25605245)
• MiR-196b and miR-1290 targeted the 3’ untranslated region of HIV-1 and affected its expression. (PMID:26469550)
• This study identified HOXA9 as a target gene of miR-196b and determined that the mechanism of miR-196b-mediated epithelial-to-mesenchymal transition and invasion processes involves the regulation of HOXA9 expression in non-small cell lung cancer cells. (PMID:26586336)
• The high level of Mir-196b expression is correlated with less chemotherapy resistance and better survival of colon cancer patients. (PMID:26626874)
• these studies highlight miR-196b as a tumor suppressor whose silencing early in lung carcinogenesis may provide a selective growth advantage to premalignant cells. (PMID:27302168)
• Data suggest that miR-124-3p, miR-9-3p and miR-196b-5p may be potential signatures for differential diagnosis of thyroid nodules in eastern coastal areas of China. (PMID:27705935)
• Data show that miR-196b is significantly upregulated in a cohort of sporadic colon cancer patients. Its upregulation was found to correlate with early stages of disease progression and with a marked reduction in GATA6 expression in colon cancer samples. (PMID:27902469)
• MiR-196b inhibitor decreased cell proliferation. (PMID:28186267)
• over-expression of miR-196b-5p may be closely associated with the risk of transformation to leukemia in myelodysplastic syndrome patients. (PMID:28224273)
• Homeobox A9 (HOXA9) expression was inversely correlated with microRNA miR-196b levels in recurrent epithelial ovarian cancer (EOC). (PMID:28387653)
• miR-196b-5p inhibition led to significantly increased colorectal cancer cell migration/invasion and metastases formation in mice, whereas ectopic overexpression showed the opposite phenotype. Molecular profiling and target confirmation identified an interaction between miR-196b-5p and HOXB7 and GALNT5, which in turn regulated colorectal cancer cell migration (PMID:28533224)
• miR-196b-5p plays an important role in the stemness and chemotherapy resistance of CRC cells via activating STAT3 signaling pathway. Importantly, miR-196b-5p was highly enriched in the serum exosomes of patients with CRC compared to healthy control subjects. (PMID:28591704)
• our data showed that miR-196b expression is up-regulated in PC tissues, and miR-196b regulate apoptosis and proliferation of PANT-1 cells via targeting CADM1. (PMID:28904340)
• Overexpression of miR-196b suppressed cell viability, migration, invasion, and induced apoptosis as well as inhibited TGF-beta induced epithelial mesenchymal transition process in A549 cells. In addition, Runx2 was a putative target of miR-196b, and Runx2 silence remarkably increased cell apoptosis and abolished the promotive effects of miR-196b suppression on cell viability, migration and invasion. (PMID:28950255)
• The early occurrence and prevalence of miR-196b dysregulation in HNSCC tumorigenesis. (PMID:29259267)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir196b	ENSMUSG00000065443

Paralogs (2): MIR196A2 (ENSG00000207924), MIR196A1 (ENSG00000210741)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.