MIR200A

Summary

MIR200A (microRNA 200a, HGNC:31578) is a microRNA gene on chromosome 1p36.33.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406983 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31578
Approved symbol	MIR200A
Name	microRNA 200a
Location	1p36.33
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-200a
Ensembl gene	ENSG00000207607
Ensembl biotype	miRNA
OMIM	612090
Entrez	406983
RNAcentral	URS000075A884 — miRNA, 90 nt, 3 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384875

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384875 — 1 exons

Exon	Start	End
ENSE00001499882	1167863	1167952

Expression profiles

Bgee: expression breadth broad, 91 present calls, max score 92.84.

Top tissues by expression

91 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of stomach	UBERON:0001161	92.84	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	91.79	gold quality
stomach	UBERON:0000945	91.43	gold quality
fundus of stomach	UBERON:0001160	89.88	gold quality
transverse colon	UBERON:0001157	89.62	gold quality
pituitary gland	UBERON:0000007	89.25	gold quality
right uterine tube	UBERON:0001302	87.99	gold quality
endometrium	UBERON:0001295	87.90	gold quality
body of pancreas	UBERON:0001150	87.85	gold quality
colon	UBERON:0001155	86.73	gold quality
small intestine Peyer’s patch	UBERON:0003454	86.42	gold quality
small intestine	UBERON:0002108	86.33	gold quality
intestine	UBERON:0000160	86.29	gold quality
adenohypophysis	UBERON:0002196	86.22	gold quality
pancreas	UBERON:0001264	86.01	gold quality
metanephros cortex	UBERON:0010533	84.98	gold quality
esophagus mucosa	UBERON:0002469	84.22	gold quality
tonsil	UBERON:0002372	83.76	gold quality
kidney	UBERON:0002113	83.50	gold quality
adult mammalian kidney	UBERON:0000082	83.14	gold quality
esophagus	UBERON:0001043	82.96	gold quality
skin of abdomen	UBERON:0001416	82.81	gold quality
uterus	UBERON:0000995	82.43	gold quality
lower esophagus mucosa	UBERON:0035834	81.50	gold quality
mucosa of transverse colon	UBERON:0004991	81.05	gold quality
zone of skin	UBERON:0000014	80.76	gold quality
mucosa of stomach	UBERON:0001199	80.24	gold quality
muscle layer of sigmoid colon	UBERON:0035805	79.66	gold quality
prostate gland	UBERON:0002367	79.61	gold quality
duodenum	UBERON:0002114	79.52	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.27

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ZEB2

Literature-anchored findings (GeneRIF, showing 40)

• study showed miR-200a, miR-200b & miR-429 in miR-200b-429 cluster are significantly associated with cancer recurrence & overall survival in advanced ovarian cancer; study suggests miR-200 miRNAs could play an important regulatory role in ovarian cancer (PMID:19501389)
• Members of the miR-200 family appear to control the EMT process and sensitivity to EGFR therapy in bladder cancer cells and the expression of miR-200 is sufficient to restore EGFR dependency at least in some of the mesenchymal bladder cancer cells. (PMID:19671845)
• miR-200a appears to act as a multifunctional tumor suppressor miRNA in meningiomas through effects on the E-cadherin and Wnt/beta-catenin signaling pathways. (PMID:19703993)
• ZEB2 and CTNNB1 are the functional downstream targets of miR-200a and they play distinct roles in regulating nasopharyngeal carcinoma development. (PMID:19931509)
• miR-200a is likely to affect the metastatic potential of cervical cancer cells by coordinate suppression of multiple genes controlling cell motility. (PMID:20124485)
• Coordinated epigenetic repression of the miR-200 family and miR-205 is associated with invasive bladder cancer. (PMID:20473948)
• miR-200bc/429 cluster targets PLCgamma1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer. (PMID:20514023)
• Most pancreatic cancers display hypomethylation and overexpression of miR-200a and miR-200b, silencing of SIP1 by promoter methylation, and retention of E-cadherin expression. (PMID:20551052)
• knockdown of miR-200a promotes epithelial-mesenchymal to stem-like transition via ZEB2 and beta-catenin signaling and results in nasopharyngeal carcinoma. (PMID:20826811)
• miR-200 family controls beta-tubulin III expression and is associated with paclitaxel-based treatment response and progression-free survival in ovarian cancer. (PMID:21051560)
• Data suggest that miR-200 suppresses lung tumorigenesis by targeting Flt1. (PMID:21115742)
• We found a significant down-regulation of the miR-200 family and miR-205, loss of desmosomal cadherins, and an altered expression of classic cadherins in spindle cell carcinoma (PMID:21237487)
• Data show that siRNA-mediated knockdown of DCAMKL-1 in pancreatic cancer cells induced microRNA miR-200a, an EMT inhibitor, along with downregulation of EMT-associated transcription factors. (PMID:21285251)
• Results suggest that loss of expression of miR-200a may play a critical role in the repression of E-cadherin by ZEB2, thereby enhancing migration and invasion in CD133/1+ cells. (PMID:21529905)
• a critical tumor suppressive role of the miR-200 family in breast epithelium in addition to identifying a novel regulatory mechanism, which may contribute to SIRT1 up-regulation in breast cancer (PMID:21596753)
• Data show that miR-200a can directly bind to thrombospondin 1 3’UTR and negatively regulate thrombospondin 1 expression. (PMID:21698760)
• HDAC4/Sp1/miR-200a regulatory network induces the down-regulation of miR-200a and the up-regulation of HDAC4 in hepatocellular carcinoma. (PMID:21837748)
• miR-200a regulates the Keap1/Nrf2 pathway in mammary epithelium (PMID:21926171)
• Data show that miR-200-regulated ZEB1 is reduced in LY2 cells. (PMID:21955614)
• Data indicate that STAT4 might be the molecular target of miR-132, miR-212, and miR-200a. (PMID:22077060)
• Our data suggest that miR-9 and miR-200a can distinguish between hemangioblastomas and metastatic clear cell renal cell carcinomas (PMID:22082152)
• miR-141 and miR-200a target p38alpha and modulate the oxidative stress response in ovarian cancers. (PMID:22101765)
• possible mechanistic role for miR-200a in progression of advanced prostate cancer (PMID:22161972)
• We demonstrated that miRNAs were dysregulated in conjunctival MALT lymphoma, and dysregulation of the miR-200 family could be involved in the pathogenesis and progression of the disease. (PMID:22183793)
• TGF-beta1-induces fibrosis in vitro and inhibits the expression of miR-200a and miR-200b in intestinal epithelial cells. (PMID:22294131)
• Data suggest an important role of miR-200a in the decline in progesterone receptor (PR) function leading to labor. (PMID:22529366)
• Overexpression of the miR-200a is associated with hepatocellular carcinoma cell migration through the epithelial to mesenchymal transition. (PMID:22868917)
• MicroRNAs miR-146a1, miR-155_2, and miR-200a1 are regulated in autoimmune thyroid diseases. (PMID:22957494)
• Upregulation of MIR200a is associated with recurrent endometrial carcinoma. (PMID:22987275)
• Results indicate that miR-200a, miR-200a*, miR-200b and miR-200b* are frequently lost in clear cell renal cell carcinoma (ccRCC). (PMID:23074016)
• ZEB1/2 upregulation by WASF3 results from downregulation of KISS1, leading to the release of inhibition of nuclear factor (NF)kappaB by IkappaBalpha (PMID:23318438)
• GNA13 is an important mediator of prostate cancer cell invasion, and miR-182 and miR-200 family members regulate its expression post-transcriptionally (PMID:23329838)
• we found that YAP1 expression was inversely correlated with miR-200a expression in breast cancer clinical specimens, and miR-200a expression was associated with distant metastasis in patients with breast cancer. (PMID:23340296)
• Studies indicate that the miR-200 family is both a marker and a powerful regulator of epithelial-to-mesenchymal transition (EMT) in cancer. (PMID:23455327)
• These results indicate that the miR-200 family plays a crucial role in the transition between stem-like and non-stem phenotypes and that distinct epigenetic-based mechanisms regulate each miR-200 gene in this process. (PMID:23525011)
• the ‘Focal adhesion’ and ‘ErbB signaling’ pathways were significantly regulated by miR-200b/c/429 and miR-200a/141, respectively. (PMID:23635949)
• High MiR200a expression is associated with metastasis and invasion of cervical carcinoma. (PMID:23679328)
• restoring miR-200a or miR-200c in H1299 cells induces downregulation of DLC1, ATRX and HFE. (PMID:23708087)
• The expression levels of miR-200a/b might determine the therapeutic efficacy of curcumin in liver neoplasms. (PMID:23760980)
• Studies indicate that three miRNA biomarkers miR-21, miR-155, and miR-200 have been repetitively identified in pancreatic cancer tissue. (PMID:23774697)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir141	ENSDARG00000080489
danio_rerio	mir200a	ENSDARG00000081399
mus_musculus	Mir200a	ENSMUSG00000065400
rattus_norvegicus	Mir3548	ENSRNOG00000035650
drosophila_melanogaster	mir-8	FBGN0262432

Paralogs (2): MIR200C (ENSG00000207713), MIR200B (ENSG00000207730)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.