MIR200B

Summary

MIR200B (microRNA 200b, HGNC:31579) is a microRNA gene on chromosome 1p36.33.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406984 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31579
Approved symbol	MIR200B
Name	microRNA 200b
Location	1p36.33
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-200b
Ensembl gene	ENSG00000207730
Ensembl biotype	miRNA
OMIM	612091
Entrez	406984
RNAcentral	URS000075C8DF — miRNA, 95 nt, 4 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384997

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384997 — 1 exons

Exon	Start	End
ENSE00001500004	1167104	1167198

Expression profiles

Bgee: expression breadth broad, 81 present calls, max score 97.34.

Top tissues by expression

81 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
olfactory segment of nasal mucosa	UBERON:0005386	97.34	gold quality
body of stomach	UBERON:0001161	93.80	gold quality
stomach	UBERON:0000945	92.28	gold quality
pituitary gland	UBERON:0000007	91.57	gold quality
body of pancreas	UBERON:0001150	90.57	gold quality
adenohypophysis	UBERON:0002196	89.37	gold quality
transverse colon	UBERON:0001157	89.29	gold quality
fundus of stomach	UBERON:0001160	89.25	gold quality
right uterine tube	UBERON:0001302	87.50	gold quality
colon	UBERON:0001155	86.52	gold quality
esophagus mucosa	UBERON:0002469	86.20	gold quality
intestine	UBERON:0000160	85.57	gold quality
skin of abdomen	UBERON:0001416	85.54	gold quality
metanephros cortex	UBERON:0010533	84.75	gold quality
small intestine Peyer’s patch	UBERON:0003454	84.68	gold quality
small intestine	UBERON:0002108	84.54	gold quality
zone of skin	UBERON:0000014	83.10	gold quality
monocyte	CL:0000576	82.96	gold quality
prostate gland	UBERON:0002367	82.44	gold quality
lower esophagus mucosa	UBERON:0035834	82.34	gold quality
kidney	UBERON:0002113	82.20	gold quality
adult mammalian kidney	UBERON:0000082	81.52	gold quality
skin of leg	UBERON:0001511	81.25	gold quality
endocervix	UBERON:0000458	80.72	gold quality
endometrium	UBERON:0001295	80.46	gold quality
saliva-secreting gland	UBERON:0001044	80.36	gold quality
minor salivary gland	UBERON:0001830	80.05	gold quality
duodenum	UBERON:0002114	78.93	gold quality
vagina	UBERON:0000996	78.57	gold quality
uterus	UBERON:0000995	78.15	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.54

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ZEB2

Literature-anchored findings (GeneRIF, showing 40)

• This study identifies miR-200b as a post-transcriptional regulator of ZFHX1B and demonstrates the ability of miR-200b to affect the promoter activity of the ZFHX1B target gene E-cadherin. (PMID:17585049)
• study showed miR-200a, miR-200b & miR-429 in miR-200b-429 cluster are significantly associated with cancer recurrence & overall survival in advanced ovarian cancer; study suggests miR-200 miRNAs could play an important regulatory role in ovarian cancer (PMID:19501389)
• Members of the miR-200 family appear to control the EMT process and sensitivity to EGFR therapy in bladder cancer cells and the expression of miR-200 is sufficient to restore EGFR dependency at least in some of the mesenchymal bladder cancer cells. (PMID:19671845)
• Data show that three members of the microRNA-200 family, miR200b, miR200c, and miR429 were predicted to have the highest scores. (PMID:19801681)
• miR-200bc/429 cluster targets PLCgamma1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer. (PMID:20514023)
• Most pancreatic cancers display hypomethylation and overexpression of miR-200a and miR-200b, silencing of SIP1 by promoter methylation, and retention of E-cadherin expression. (PMID:20551052)
• Data show that expression of MIR152, MIR200B, and MIR338 is specifically modulated in neuroblastoma cell lines during differentiation and apoptosis. (PMID:20574809)
• miRNAs 200b and 429 are key regulators via their effects on expression of ZEB1 and ZEB2 of the switch between latent and lytic infection by EBV (PMID:20668090)
• study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target (PMID:20683643)
• Loss of miR-200 during CSC formation increases Suz12 expression, Suz12 binding, H3-K27 trimethylation, and Polycomb-mediated repression of the E-cadherin gene. (PMID:20832727)
• results indicate that miR-200 family is up-regulated after ureter obstruction, miR-200b being strongly induced, and that miR-200b ameliorates tubulointerstitial fibrosis in obstructed kidneys (PMID:21049046)
• miR-200 family controls beta-tubulin III expression and is associated with paclitaxel-based treatment response and progression-free survival in ovarian cancer. (PMID:21051560)
• These findings implicate the miR-200 family and their targets, ZEB1 and ZEB2, as unique regulators of uterine quiescence and contractility during pregnancy and labor. (PMID:21079000)
• hypoxia-sensitive miR-200b is involved in induction of angiogenesis via directly targeting Ets-1 in HMECs. (PMID:21081489)
• We found a significant down-regulation of the miR-200 family and miR-205, loss of desmosomal cadherins, and an altered expression of classic cadherins in spindle cell carcinoma (PMID:21237487)
• data suggest that reduced expression of miR-200b and miR-15b underscores the mechanisms of chemotherapy-induced epithelial-mesenchymal transition in tongue squamous cell carcinoma (PMID:21725369)
• Our results suggest that down-regulation of miR-200b could lead to E2F3 overexpression and in turn contribute to chemoresistance of lung adenocarcinoma cells to docetaxel. (PMID:22139708)
• We demonstrated that miRNAs were dysregulated in conjunctival MALT lymphoma, and dysregulation of the miR-200 family could be involved in the pathogenesis and progression of the disease. (PMID:22183793)
• These data suggest an association between miR-200b regulation and breast cancer subtype and a potential use of DNA methylation of miRNA promoters as a component of a suite of breast cancer biomarkers. (PMID:22231446)
• TGF-beta1-induces fibrosis in vitro and inhibits the expression of miR-200a and miR-200b in intestinal epithelial cells. (PMID:22294131)
• miR-200b regulates zinc finger E-box-binding homeobox 2 (ZEB2) expression and thus controls metastasis in gastric cancer. (PMID:22311119)
• These data define miR-200b and miR-200c as factors that modify the efficiency of TLR4 signaling through the MyD88-dependent pathway and can thus affect host innate defenses against microbial pathogens. (PMID:22522429)
• Data indicate that methylation frequency of 5 miR CpG islands (miR-9-1, miR-9-3, miR-137, miR-34b, and miR-210) gradually increased while the proportion of methylated miR-200b gradually decreased during gastric carcinogenesis. (PMID:22703336)
• Data indicate that in KRAS mutated tumours increased miR-200b and decreased miR-143 expression were associated with a good progression-free survival (PFS) upon treatment. (PMID:22804917)
• Overexpression of the miR-200b is associated with hepatocellular carcinoma cell migration through the epithelial to mesenchymal transition. (PMID:22868917)
• Results indicate that miR-200a, miR-200a*, miR-200b and miR-200b* are frequently lost in clear cell renal cell carcinoma (ccRCC). (PMID:23074016)
• High miR-200b expression is associated with aggressiveness of muscle-invasive bladder cancer. (PMID:23169479)
• miR-200b enhances matrix metallopeptidase 2 (MMP2) activity by downregulating TIMP2 expression in endometrial adenocarcinoma HEC-1A cells (PMID:23205572)
• analysis of an antagonistic function of the RNA-binding protein HuR and miR-200b in post-transcriptional regulation of vascular endothelial growth factor-A expression and angiogenesis (PMID:23223443)
• ZEB1/2 upregulation by WASF3 results from downregulation of KISS1, leading to the release of inhibition of nuclear factor (NF)kappaB by IkappaBalpha (PMID:23318438)
• miR-200b-3p is down-regulated by low expression of p73 in androgen-independent prostate cancer cells, and this interaction contributes to the proliferation of AIPC. (PMID:23389960)
• Studies indicate that the miR-200 family is both a marker and a powerful regulator of epithelial-to-mesenchymal transition (EMT) in cancer. (PMID:23455327)
• Kindlin 2 plays a novel role in epigenetic repression of miR-200 family, a mechanism that promotes breast cancer invasion. (PMID:23483548)
• Mir-200b maintains intact intestinal epithelium through inhibiting epithelial-mesenchymal transition and promoting proliferation of intestinal epithelial cells. (PMID:23492772)
• MiR-200b targets the CREB1 gene and suppresses glioma cell growth. (PMID:23543137)
• the ‘Focal adhesion’ and ‘ErbB signaling’ pathways were significantly regulated by miR-200b/c/429 and miR-200a/141, respectively. (PMID:23635949)
• Reinforced miR-200b expression results in downregulation of DLC1, HNRNPA3 and HFE. (PMID:23708087)
• The expression levels of miR-200a/b might determine the therapeutic efficacy of curcumin in liver neoplasms. (PMID:23760980)
• Studies indicate that three miRNA biomarkers miR-21, miR-155, and miR-200 have been repetitively identified in pancreatic cancer tissue. (PMID:23774697)
• Wnt-1 is a target of regulation by miR-200b and miR-22. (PMID:23851184)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir200c	ENSDARG00000080713
danio_rerio	mir200b	ENSDARG00000081868
mus_musculus	Mir200b	ENSMUSG00000065549

Paralogs (2): MIR200A (ENSG00000207607), MIR200C (ENSG00000207713)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.