MIR20A

Summary

MIR20A (microRNA 20a, HGNC:31577) is a microRNA gene on chromosome 13q31.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406982 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31577
Approved symbol	MIR20A
Name	microRNA 20a
Location	13q31.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-20, hsa-mir-20a
Ensembl gene	ENSG00000283762
Ensembl biotype	miRNA
OMIM	609420
Entrez	406982
RNAcentral	URS0000507C9B — miRNA, 71 nt, 56 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362279

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362279 — 1 exons

Exon	Start	End
ENSE00001437042	91351065	91351135

Expression profiles

Bgee: expression breadth tissue_specific, 2 present calls, max score 60.45.

Top tissues by expression

2 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
skin of leg	UBERON:0001511	60.45	gold quality
prostate gland	UBERON:0002367	52.07	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• expression of E2F1 is negatively regulated by two miRNAs in the cluster, miR-17-5p and miR-20a (PMID:15944709)
• E2F1, E2F2, and E2F3 directly bind the promoter of the mir-17-92 cluster, activating its transcription. miR-20a from the mir-17-92 cluster in turn modulates the translation of E2F2 and E2F3, suggesting an autoregulatory feedback loop. (PMID:17135249)
• a circuit involving c-myc, miR-17-92, and HIF-1 alpha may play a role in cancer cell proliferation under normoxia in a cellular context-dependent manner [miR-92] (PMID:18632605)
• These studies identify a novel cyclin D1/miR-17/20 regulatory feedback loop through which cyclin D1 induces miR-17-5p/miR-20a. (PMID:18695042)
• miRNA 17-5p, 20a and 126 are constitutively expressed in Ph(-) MPN megakaryopoiesis while low or absent miRNA 10a appeared to correlate with strong megakaryocytic HOXA1 protein expression (PMID:18773208)
• miR-17 and miR-20a govern the transition through G1 in normal diploid human cells. (PMID:18836483)
• HIF-1alpha and VEGF regulation by miR-20b tunes tumor cells to adapt to the alteration of oxygen concentration (PMID:19893619)
• identified four commonly regulated miRNAs, miR-17 down-regulated in all seven; miR-19b and miR-20a, down-regulated in six models; and miR-106a down-regulated in five models. (PMID:20089119)
• microRNA17/20 cluster is shown to govern cellular migration and invasion of nearby cells via heterotypic secreted signals. (PMID:20406904)
• Down regulation of miR-20b is associated with mantle cell lymphoma (PMID:20485376)
• microRNA-20a regulates Stat3 at the post-transcriptional level, resulting in inhibition of cell proliferation and invasion of pancreatic carcinoma (PMID:20583868)
• miR-17 and miR-20a were significantly under-expressed in multiple sclerosis (PMID:20711463)
• more dedifferentiated prostate cancer cells have a higher expression of miR-20a and this supports the oncogenic role of miR-20a in prostate cancer carcinogenesis (PMID:20944140)
• Our findings suggested that miR-20a may play a role in colorectal adenocarcinoma cancer cell drug resistance (PMID:21242194)
• Mir-17, -20a, and -106b downregulate a common set of pro- and anti-proliferative target genes to impact cell cycle progression of human cord blood stem cells and increase intracellular activity of E2F transcription factors to govern G(1)/S transition. (PMID:21283765)
• miR-20a promoted the osteogenesis of mesenchymal stem cells in a co-regulatory pattern by targeting PPAR-gamma, Bambi and crim1, the negative regulators of BMP signaling (PMID:21743293)
• Data show that miR-17/20 inhibition suppresses proliferation in cultured retinoblastoma cells. (PMID:21816922)
• miR-20a, encoded by miR-17-92, downregulates Fas expression in osteosarcoma, thus contributing to the metastatic potential of osteosarcoma cells by altering the phenotype and allowing survival in the FasL(+) lung microenvironment. (PMID:22186140)
• miR-20a shows potent effect in promoting the growth of glioma cells (PMID:22381757)
• miR-17, miR-20a, and miR-20b are differentially regulated in human placentas by preeclampsia. (PMID:22438230)
• Data suggest that miR-20a can promote migration and invasion of cervical cancer cells through the upregulation of TNKS2. (PMID:22449978)
• miR20a is an important modulator in the development of endometriosis. (PMID:22648654)
• Study reports the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 A resolution; the miRNA is anchored at both ends by the Mid and PAZ domains and makes several kinks and turns along the binding groove and miRNA binding confers remarkable stability on hAgo2. (PMID:22682761)
• miR-20a acts in a feedback loop to repress the expression of MKK3 and to negatively regulate the p38 pathway-mediated VEGF-induced endothelial cell migration and angiogenesis (PMID:22696064)
• establish that p53 directly regulates Quaking (QKI) gene expression, and QKI protein associates with and leads to the stabilization of miR-20a (PMID:22751500)
• miR-20a significantly contributes to the progression of prostate cancer by targeting CX43. (PMID:22785209)
• miR-20a and miR-106b (known to be closely related) were the two most robust upregulated miRNAs in pediatric brainstem gliomas compared to adult subtype (PMID:22825541)
• Low miR-20a is associated with breast cancer metastasis. (PMID:22901144)
• 4 miRNAs including miR-17, miR-20a, miR-29c, and miR-223 were found to be expressed differentially in the serum of NPC compared with that of non-cancerous control (PMID:23056289)
• The mir-20a can target the p21 and STAT3 3’ UTR to inhibit the expression of p21 and STAT3 in human cells. (PMID:23059786)
• Data point toward an important role for miR-20a in the regulation of pro-inflammatory cytokines release, by controlling ASK1 expression in rheumatoid fibroblast-like synoviocytes. (PMID:23087182)
• Plasma miR-17-5p, miR-20a and miR-22 are down-regulated in women with endometriosis compared with those without endometriosis. (PMID:23203215)
• mir-17-5p and mir-20a constitute favorable prognostic factors in myelodysplastic syndromes. (PMID:23246221)
• Plasma miR-106b, miR-20a, and miR-221 have the potential as novel, non-invasive biomarkers for the early detection of gastric cancer (PMID:23307259)
• Fndings indicate that miR-17-5p/20a promote gastric cancer cell proliferation and inhibit cell apoptosis via post-transcriptional modulation of p21 and TP53INP1. (PMID:23333058)
• High miR-20a expression was associated with higher complete remission rates & overall survival in Chinese adult patients with de novo acute myeloid leukemia. (PMID:23515710)
• These findings demonstrate for the first time that miR-17, miR-20a, and miR-106a regulate SIRPalpha synthesis and SIRPalpha-mediated macrophage inflammatory responses in a redundant fashion. (PMID:23562609)
• MiR-20a is decreased in HCCs and correlates with HCC recurrence and prognosis. Down-regulation of miR-20a increases the proliferation abilities of HCC cells. (PMID:23594563)
• miR-17/20a acts as a prognostic predictor of OSCC patients’ outcome and a tumor migration suppressor miRNA (PMID:23602254)
• TGF-beta1-mediated activation of the miR-20a/Smad7/beta-catenin axis plays a pivotal role in the pathogenesis and worse prognosis of gallbladder carcinoma (PMID:23665284)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir20b	ENSDARG00000082177
danio_rerio	dre-mir-20a	ENSDARG00000104910
mus_musculus	Mir20a	ENSMUSG00000065442
rattus_norvegicus	Mir20a	ENSRNOG00000035605

Paralogs (4): MIR106B (ENSG00000208036), MIR18A (ENSG00000283815), MIR20B (ENSG00000284043), MIR17 (ENSG00000284536)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.