MIR20B

Summary

MIR20B (microRNA 20b, HGNC:32024) is a microRNA gene on chromosome Xq26.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574032 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32024
Approved symbol	MIR20B
Name	microRNA 20b
Location	Xq26.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-20b
Ensembl gene	ENSG00000284043
Ensembl biotype	miRNA
OMIM	300950
Entrez	574032
RNAcentral	URS000075BB8A — miRNA, 69 nt, 8 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384977

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384977 — 1 exons

Exon	Start	End
ENSE00001499984	134169809	134169877

Expression profiles

Top tissues by expression

0 total, by Bgee expression score (0-100, higher = more expressed):

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): EGR1

Literature-anchored findings (GeneRIF, showing 40)

• VEGF expression in breast cancer cells is mediated by HIF-1 and STAT3 in a miR-20b-dependent manner. (PMID:20232316)
• Underexpression of miR-148a and miR-20b in human embryonic stem cells (hESs) were differentiated toward Mesenchymal stem cell, compared with ESs, allows an increase in expression of the EPAS1. (PMID:21081659)
• miR-17, miR-20a, and miR-20b are differentially regulated in human placentas by preeclampsia. (PMID:22438230)
• Low miR-20b is associated with breast cancer metastasis. (PMID:22901144)
• Results indicate that upregulated miR-20b, miR-9, and miR-9* were significantly associated with HPV/p16-status. (PMID:23459718)
• Findings demonstrate that EGR1 is a key player in the transcriptional control of miR-20b, and miR-20b may in turn function as an oncogene by contributing to breast tumorigenesis via tumor suppressor targeting. (PMID:23945289)
• PAR-1 is post-transcriptionally regulated by miR-20b microRNA in human melanoma cells. Endogenous melanoma microRNAs interacted with PAR-1 3’-UTR to silence a fused reporter. Transfection of a miR-20b inhibitor into primary melanoma cells reversed this. (PMID:24405508)
• The miR-20b downregulated the TF expression independently of the Erk1/2 signaling pathway. (PMID:24405935)
• MiR-20b, -21, and -130b inhibit PTEN expression resulting in B7-H1 over-expression in advanced colorectal cancer. (PMID:24468585)
• our study suggests that miRNA 17 family (including miR-17, 20a, 20b) can act as TGFbetaR2 suppressor for reversing cisplatin-resistant and suppressing metastasis in non small cell lung cancer. (PMID:24722426)
• Underexpression of miR-126 and miR-20b occurs as an early event of colorectal carcinogenesis in familial adenomatous polyposis tumors. (PMID:24994098)
• we report the novel concept that miR-20b exerts a suppressive effect on both cell cycle-modulated proliferation and MMP-2-mediated migration and invasion in bladder cancer EJ cells. (PMID:26166554)
• expression of miR-20b is associated with clinicopathological characteristics and overall survival of HCC patients . HIF-1alpha and VEGF targets of miR-20b have been confirmed. (PMID:26612965)
• miR20b5p may be a biomarker for early detection and prognosis prediction, as well as a therapeutic target for Renal cell carcinoma. (PMID:26708577)
• MiR-20a and miR-20b negatively regulate autophagy by targeting RB1CC1/FIP200 in breast cancer cells (PMID:26829385)
• MiR-20b, an immune- and cancer-related miRNA, is decreased in the serum of MG patients. (PMID:26845056)
• Transfection of miRNA mimics for unexpressed members of the miR-106a-363 cluster (miR20b, miR-363-3p and miR-363-5p) exhibit an anti-proliferative effect on oral carcinoma cells, although likely mediated by different regulatory mechanisms. (PMID:27001184)
• Results suggest that the upregulation of miR-20b affects the expression of HIF-1alpha, downregulates the VEGF pathway proteins, and suppresses cell invasion and proliferation rate of osteosarcoma. (PMID:27098149)
• showed that miR-20b was down-regulated in the retina and retinal endothelial cells in diabetic rats, with a correlated up-regulation of VEGF and AKT3 (PMID:27421659)
• MIR20b, MIR498 and MIR196 are involved in both apoptosis and autophagy processes in esophageal squamous cell carcinoma (PMID:27462775)
• miR-125b/miR-20b and Wnt signalling have roles in glioblastoma phenotypes in a pathway that involves FZD6 (PMID:27698350)
• These results indicate for the first time that miR-20b displays tumor-suppressor functions in papillary thyroid cancer. By targeting SOS1 and ERK2, miR-20b inhibits the activity of the MAPK/ERK signaling pathway. The findings suggest that miR-20b may play an important role in papillary thyroid cancer initiation, progression, and metastasis. (PMID:27717302)
• loss of miR-17 and miR-20b enhanced breast cancer resistance to taxol by upregulating NCOA3 levels. (PMID:27831559)
• Conclusion. miR-20b acts as a tumor suppressor in the development of thymoma and thymoma-associated myasthenia gravis. The tumor suppressive function of miR-20b in thymoma could be due to its inhibition of NFAT signaling by repression of NFAT5 and CAMTA1 expression. (PMID:27833920)
• ADAM9 is a direct target of miR-20b and that miR-20b decreased the 5-FU resistance of HCT116-R cells. (PMID:27878272)
• Chronic exposure to TNF-alpha causes premature senescence of endothelial cells with the involvement of hsa-miR-20b and its target gene, RBL1. (PMID:28595801)
• that miR-20b could alleviate the inflammatory response in Tuberculosis mice via targeting the NLRP3/caspase-1/IL-1beta pathway (PMID:28606793)
• This study demonstrated that the Overexpression of miR-20b downregulated the expressions of Ngn2, MAP2, and TUBB3. miR-20b may directly or indirectly regulate neuronal genes expression to modulate the neural differentiation of human umbilical cord mesenchymal stem cells. (PMID:29016394)
• Microarray-based analyses revealed that the expression of miR-20b was significantly increased, whereas TGFBR2 and MYC were significantly downregulated and upregulated, respectively, in all ES cells compared to their expression in human mesenchymal stem cells (hMSCs). (PMID:29039480)
• Our study suggests that miR-20b-5p may play an important role in airway remodeling during asthma (PMID:29549727)
• miR-20b-5p overexpression retains its favorable prognostic role in CLL patients of intermediate risk or stratified according to established prognostic factors [CD38 expression and mutational status of the immunoglobulin heavy chain variable (IGHV) region]. In conclusion, miR-20b-5p is a potential independent molecular biomarker of favorable prognosis in CLL. (PMID:29715621)
• A significant decrease in serum miR-20b and miR-17-3p and a significant increase in serum HOTAIR and MALAT1 in proliferative diabetic retinopathy relative to non-proliferative diabetic retinopathy has been observed. (PMID:30468285)
• The expression of miR-20b-5p is negatively correlated with that of metastasis-associated lung adenocarcinoma transcript-1 (MALAT1, r = -0.928, p = 0.023) and Oct4 (r = -0.894, p = 0.041) in colorectal cells. (PMID:31012108)
• Study found that an unreported lncRNA in glioblastoma, lnc-TALC (temozolomide-associated lncRNA in glioblastoma recurrence), correlated with temozolomide resistance via competitively binding miR-20b-3p to facilitate c-Met expression. (PMID:31053733)
• Adipose derived stem cells promote tumor metastasis in breast Cancer cells by stem cell factor inhibition of miR20b. (PMID:31254605)
• miR-20b is identified to regulate intestinal FPN expression in vitro and in vivo, which will provide a potential target for intestinal iron exportation. (PMID:31554201)
• Downregulation of circDNMT3B contributes to vascular dysfunction in diabetic retinas through regulating miR-20b-5p and BAMBI, providing a potential treatment strategy for diabetic retinopathy (PMID:31636010)
• Circulating Exosomal miR-20b-5p Inhibition Restores Wnt9b Signaling and Reverses Diabetes-Associated Impaired Wound Healing. (PMID:31867895)
• miR20b promotes growth of nonsmall cell lung cancer through a positive feedback loop of the Wnt/betacatenin signaling pathway. (PMID:31894264)
• miR20b inhibits the senescence of human umbilical vein endothelial cells through regulating the Wnt/betacatenin pathway via the TXNIP/NLRP3 axis. (PMID:31922218)

Cross-species orthologs

0 orthologs

Paralogs (4): MIR106B (ENSG00000208036), MIR20A (ENSG00000283762), MIR18A (ENSG00000283815), MIR17 (ENSG00000284536)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.