MIR214

Summary

MIR214 (microRNA 214, HGNC:31591) is a microRNA gene on chromosome 1q24.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 406996 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31591
Approved symbol	MIR214
Name	microRNA 214
Location	1q24.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-214
Ensembl gene	ENSG00000283844
Ensembl biotype	miRNA
OMIM	610721
Entrez	406996
RNAcentral	URS0000160683 — miRNA, 110 nt, 47 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385214

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385214 — 1 exons

Exon	Start	End
ENSE00001500220	172138798	172138907

Expression profiles

Bgee: expression breadth broad, 55 present calls, max score 79.26.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0994 / max 6.6487, expressed in 41 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
15905	0.0994	41

Top tissues by expression

55 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
liver	UBERON:0002107	79.26	gold quality
ovary	UBERON:0000992	78.84	gold quality
blood	UBERON:0000178	75.59	gold quality
right adrenal gland cortex	UBERON:0035827	73.36	gold quality
gastrocnemius	UBERON:0001388	73.08	gold quality
muscle of leg	UBERON:0001383	72.89	gold quality
skeletal muscle tissue	UBERON:0001134	71.69	gold quality
caudate nucleus	UBERON:0001873	71.44	gold quality
right atrium auricular region	UBERON:0006631	71.26	gold quality
descending thoracic aorta	UBERON:0002345	71.01	gold quality
right hemisphere of cerebellum	UBERON:0014890	67.24	gold quality
amygdala	UBERON:0001876	67.20	gold quality
Brodmann (1909) area 9	UBERON:0013540	66.96	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.77	gold quality
right adrenal gland	UBERON:0001233	66.34	gold quality
upper lobe of left lung	UBERON:0008952	65.19	gold quality
heart	UBERON:0000948	64.80	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	64.74	gold quality
omental fat pad	UBERON:0010414	64.34	gold quality
anterior cingulate cortex	UBERON:0009835	63.44	gold quality
prostate gland	UBERON:0002367	62.73	gold quality
apex of heart	UBERON:0002098	62.51	gold quality
esophagus mucosa	UBERON:0002469	61.59	gold quality
multicellular organism	UBERON:0000468	61.57	gold quality
right frontal lobe	UBERON:0002810	61.57	gold quality
frontal lobe	UBERON:0016525	61.56	gold quality
lung	UBERON:0002048	61.51	gold quality
body of stomach	UBERON:0001161	61.14	gold quality
left adrenal gland	UBERON:0001234	61.08	gold quality
lower esophagus muscularis layer	UBERON:0035833	60.80	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): EZH2

Literature-anchored findings (GeneRIF, showing 40)

• miR-214 induces cell survival and cisplatin resistance primarily through targeting the PTEN/Akt pathway. (PMID:18199536)
• Following down-regulation of microRna-214 expression in umbilical vein endothelial cells, there is an increase in endothelial nitric oxide synthase expression and in cell migration and tube formation. (PMID:19733659)
• miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3’UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs. (PMID:19859982)
• Twist1 as a regulator of miRNA cluster responsible for the regulation of the IKKbeta/NF-kappaB and PTEN/AKT pathways and its association of ovarian cancer stem cell differentiation (PMID:20400975)
• plexin-B1, a target of miR-214, may function as an oncogene in human cervical cancer HeLa cells (PMID:21216304)
• [advanced glycation end product; AGE]Overexpression of pre-micro(mi)RNA-214 leads to impaired phosphatase and tensin homolog (PTEN) expression and delayed apoptosis of THP-1 cells, whereas miR-214 knockdown largely abolishes AGE-induced cell survival. (PMID:21228352)
• miR-214 is highly expressed in human melanomas and the data suggest a critical role for this miRNA in disease progression and the establishment of distant metastases through suppression of TFAP2C. (PMID:21468029)
• MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sezary syndrome (PMID:21525938)
• Decreased microRNA-214 levels in breast cancer cells coincides with increased cell proliferation, invasion and accumulation of the Polycomb Ezh2 methyltransferase. (PMID:21828058)
• miR-214, miR-150, miR-146a and miR-125b targeted HTT. (PMID:22048026)
• Adenosine A2A receptor upregulation in human PMNs is controlled by miRNA-214, miRNA-15, and miRNA-16 (PMID:22249219)
• circulating, cell-free miR-214 has diagnostic potential in breast cancer as indicator of malignant disease and metastatic spread to regional lymph nodes. (PMID:22350790)
• unfolded protein response may offer a new explanation for why the miR-199a/214 cluster were down-regulated in the progression in HCC. (PMID:22359598)
• miR-214 is a new regulator of GALNT7, and both miR-214 and GALNT7 play important roles in the pathogenesis of cervical cancer (PMID:22399294)
• Down-regulation of miR-214 contributes to intrahepatic cholangiocarcinoma metastasis by targeting Twist. (PMID:22540680)
• miR-214 is a tumor-suppressor of human hepatocellular carcinoma (HCC). Its downregulation is associated with worse prognosis. miR-214 downregulation contributes to hypervascularity of HCC via the induction and secretion of hepatoma-derived growth factor. (PMID:22613005)
• miR-214 is the regulator of ERK1, whereas ERK2 is regulated by miR-124 and miR-214 in cutaneous squamous cell carcinoma. (PMID:22828925)
• MicroRNA-98 and microRNA-214 post-transcriptionally regulate enhancer of zeste homolog 2 and inhibit migration and invasion in human esophageal squamous cell carcinoma. (PMID:22867052)
• We demonstrated a functional role for miRNA-214 showing that by manipulating miRNA-214 expression, the radiotherapy response of non small cell lung cancer cells can be significantly altered. (PMID:22929890)
• Down-regulation of MiR-214, up-regulation of EZH2, CTNNB1 and the down-regulation of CDH1 in hepatocellular carcinoma patients correlated with early recurrent disease and can be an independent predictor of poor survival. (PMID:22962603)
• These findings identify miR-199a-3p/miR-214 as important regulators of myometrial contractility and provide new insight into strategies to prevent preterm birth. (PMID:22973051)
• cellular miR-214 is capable of inhibiting adenovirus replication by regulating the translation of E1A protein. (PMID:22982546)
• These findings indicate that miR-214 serves as tumor suppressor and plays substantial roles in inhibiting the tumorigenesis of hepatocellular carcinoma through suppression of beta-catenin. (PMID:23068095)
• Data indicate that miR-214 down-regulated the expression of PSMD10 (gankyrin) and ASF1B, and gankyrin inhibition induced an increase of P53 mRNA levels. (PMID:23100276)
• Low miR-214 expression is associated with glioma. (PMID:23187003)
• miR-214 directly binds and regulates activity of the Necl-2 3’UTR. (PMID:23301758)
• Expression of miR-214 reduces cell survival, induces apoptosis and enhances sensitivity to cisplatin through directly inhibiting Bcl2l2 expression. (PMID:23337879)
• The expression of miR-214 is reduced in cardiac tissue from dilated cardiomyopathy patients compared to controls. (PMID:23360823)
• conclude that isoflurane increases cell death in the presence of amyloid beta by increasing Bax level through downregulating miR-214 (PMID:23408966)
• miR-214 was down-regulated in nasopharyngeal carcinoma. (PMID:23479198)
• Endothelial cells release miR-214-containing exosomes to stimulate angiogenesis through the silencing of ataxia telangiectasia mutated in neighboring target cells. (PMID:23532734)
• Downregulation of miR-124 promotes the growth and invasiveness of glioblastoma cells involving upregulation of PPP1R13L. (PMID:23624869)
• results define a pathway involving miR-214, miR-148b, TFAP2, and ALCAM that is critical for establishing distant metastases in melanoma. (PMID:23667173)
• Alcohol represses glutathine reductase and cytochrome P450 oxidoreductase expression via up-regulation of miR-214 in liver cells. (PMID:23905773)
• Studied the role of miRNA-214 in the metastatic potential of NSCLC. and identified a number of metastasis-related target genes, including PAPP-A, ALPK2, CDK6 and TNFAIP3. These were validated to be regulated by miRNA-214. (PMID:23929716)
• downregulation of miR-214 in HCC and the upregulation of its target gene FGFR-1 is associated with hepatocellular carcinoma progression (PMID:23962428)
• Human HTT gene expression regulation by Huntington’s disease variants of miR-137, miR-214, and miR-148a. (PMID:23965969)
• Upregulated expression of microRNA-214 is linked to tumor progression in pediatric osteosarcoma. (PMID:24038809)
• Exosomal transfer of miR-214 is a paradigm for the regulation of CCN2-dependent fibrogenesis and identifies fibrotic pathways as targets of intercellular regulation by exosomal miRs. (PMID:24122827)
• In kidney tissue, miR-214 was detected in cells of the glomerulus and tubules as well as in infiltrating immune cells in diseased tissue. (PMID:24158985)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir214a	ENSDARG00000083042
mus_musculus	Mir214	ENSMUSG00000065516
rattus_norvegicus	Mir214	ENSRNOG00000035513

Paralogs (1): MIR3120 (ENSG00000283152)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.