MIR218-2

Summary

MIR218-2 (microRNA 218-2, HGNC:31596) is a microRNA gene on chromosome 5q34.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407001 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31596
Approved symbol	MIR218-2
Name	microRNA 218-2
Location	5q34
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-218-2
Ensembl gene	ENSG00000207739
Ensembl biotype	miRNA
OMIM	616771
Entrez	407001
RNAcentral	URS00003735F0 — miRNA, 110 nt, 21 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385006

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385006 — 1 exons

Exon	Start	End
ENSE00001500013	168768146	168768255

Expression profiles

Bgee: expression breadth broad, 75 present calls, max score 81.78.

Top tissues by expression

75 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
olfactory segment of nasal mucosa	UBERON:0005386	81.78	gold quality
sural nerve	UBERON:0015488	75.33	gold quality
gastrocnemius	UBERON:0001388	74.44	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	73.48	gold quality
endocervix	UBERON:0000458	73.20	gold quality
caudate nucleus	UBERON:0001873	73.02	gold quality
tibial artery	UBERON:0007610	72.36	gold quality
popliteal artery	UBERON:0002250	72.35	gold quality
heart left ventricle	UBERON:0002084	71.48	gold quality
placenta	UBERON:0001987	71.38	gold quality
ascending aorta	UBERON:0001496	71.33	gold quality
thoracic aorta	UBERON:0001515	71.17	gold quality
right atrium auricular region	UBERON:0006631	70.97	gold quality
heart	UBERON:0000948	70.88	gold quality
corpus callosum	UBERON:0002336	70.86	gold quality
body of pancreas	UBERON:0001150	70.64	gold quality
stomach	UBERON:0000945	70.24	gold quality
right adrenal gland cortex	UBERON:0035827	69.97	gold quality
right lobe of liver	UBERON:0001114	69.90	gold quality
left adrenal gland cortex	UBERON:0035825	69.79	gold quality
blood	UBERON:0000178	69.77	gold quality
left adrenal gland	UBERON:0001234	69.75	gold quality
muscle layer of sigmoid colon	UBERON:0035805	69.66	gold quality
body of stomach	UBERON:0001161	69.35	gold quality
subcutaneous adipose tissue	UBERON:0002190	69.22	gold quality
left coronary artery	UBERON:0001626	69.17	gold quality
right coronary artery	UBERON:0001625	69.16	gold quality
lower esophagus muscularis layer	UBERON:0035833	69.01	gold quality
colon	UBERON:0001155	68.91	gold quality
adrenal tissue	UBERON:0018303	68.85	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.36

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• MIR218-1 and MIR218-2 are deleted and downregulated in lung squamous cell carcinoma. (PMID:20838434)
• Data show direct interaction between miR-218 and the 3’UTR of mRNAs encoding ROBO1, survivin (BIRC5), and connexin43 (GJA1). (PMID:21385904)
• Data suggest microRNA-218 was deregulated in most cervical cancer patients and associated with tumor invasion. (PMID:22237456)
• miR-218 is involved in the invasive behavior of glioblastoma cells by targeting LEF1 and blocking the invasive axis. (PMID:22766851)
• results implicate the involvement of miR-218-2 and its host gene SLIT3 in thyroid cancer cell invasion, migration, and proliferation (PMID:23720784)
• MIR218 functions as a tumor suppressor by regulating SH3GL1 expression in medulloblastoma cancer cells. (PMID:23970061)
• rs11134527 may be a novel genetic risk factor of hepatocellular carcinoma in hepatitis B virus-exposed subjects. (PMID:24118778)
• MicroRNA-218 inhibits melanogenesis by directly suppressing microphthalmia-associated transcription factor expression. (PMID:24824743)
• miR-218 is deregulated in gastric cancer patients and is strongly correlated with tumor stage, grade and metastasis. (PMID:24944481)
• Modulation of miR-218 actively affected human hepatocellular carcinoma cancer cell development (PMID:25120782)
• Report up-regulation of microRNA218 in gastric cancer after cytoreductive surgery/hyperthermic intraperitoneal chemotherapy. (PMID:25170221)
• Data suggest that pri-miR-218 rs11134527 may contribute to the genetic susceptibility and prognosis for esophageal squamous cell carcinoma in Chinese Han population. (PMID:25337271)
• Data indicate that survivin is an important target of microRNA-218 (miR-218) in cervical cancer cells. (PMID:25473903)
• MiR218 was revealed to inhibit adiponectininduced AMPK and p38 MAPK activation and glucose uptake in HepG2 cells. (PMID:25634129)
• Expression of miR-218 was not altered in cervical cancer. (PMID:25701838)
• MiR-205/miR-218 expression is associated with carboplatin chemoresistance and regulation of apoptosis via Mcl-1 and survivin signaling in lung cancer cells. (PMID:25917317)
• Identification of a miRNA, miR-218 that binds directly to the 3’-UTR of RPTPalpha. (PMID:25940608)
• miR-218 was found to suppress gastric cancer metastasis by simultaneously mediating multiple molecules in the POU2F2-oriented network. (PMID:26019213)
• Treatment with propofol efficiently reduced MMP2 protein expression levels, and overexpression of miR218 also decreased MMP2 protein expression levels. (PMID:26133092)
• Results reveal an essential role of miR-218 as a downstream effector of the Isl1-Lhx3 complex in establishing motor neuron identity. (PMID:26212498)
• These findings suggest that miR-218 inhibits multidrug resistance of gastric cancer cells by down-regulating smoothened expression. (PMID:26261515)
• miR-218 directly binds to the 3’-UTR of HMGB1 gene and up-regulates HMGB1 in paclitaxel resistant endometrial carcinoma cells. (PMID:26261543)
• downregulation of miR-218 may contribute to the chemoresistance of non-small cell lung cancer cells to cisplatin, which leads to upregulation of RUNX2 (PMID:26282001)
• MEF2D overexpression participated in the growth of lung cancers and its aberrant expression may result from the reduction of tumor suppressor miR-218. (PMID:26409449)
• High miR-218 expression had a positive prognostic value in 5-FU-based treatments for colorectal cancer patients. (PMID:26442524)
• serum expression of miR-218 might be a potential noninvasive tumor biomarker in the diagnosis and assessment of prognosis of hepatocellular carcinoma (PMID:26586116)
• miR-218 directly targets LGR4 and modulated the PI3K/Akt and Wnt/beta-catenin pathways in the LNCaP-IL-6+ cells. (PMID:26986507)
• the results of this study highlight an important role for miR-218-5p in the regulation of epidermal growth factor receptor in non-small cell lung cancer (PMID:27057632)
• miR-214 and miR-218 function as tumor suppressors in breast cancer, and may become biomarkers and potential therapeutic targets in breast cancer. (PMID:27109339)
• We found that miR-218 is downregulated in cervical cancer tissue and inhibits Epithelial-Mesenchymal Transition, migration and invasiveness of cervical cancer cells (PMID:27285984)
• results strongly suggest a role for miR-218-5p in the host response against cigarette smoke exposure and in the pathogenesis of Chronic Obstructive Pulmonary Disease (PMID:27409149)
• these findings highlight the pivotal role of miR-218 in various aspects of MACC1 expression regulation and MACC1-mediated colorectal cancer progression (PMID:27462788)
• miR-218 sensitized HCT-116/L-OHP cells to L-OHP-induced cell apoptosis via inhibition of cytoprotective autophagy by targeting YEATS4 expression. (PMID:27779719)
• mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 expression. (PMID:27974673)
• Tumor-suppressive microRNA-218 inhibits tumor angiogenesis through RICTOR/VEGFA axis in prostate tumor cells. (PMID:28030804)
• An anti-angiogenic role of miR-218 in gastric cancer (PMID:28323002)
• Describe a regulatory loop miR-218-CDK6/CyclinD1-E2F1 whose disruption may contribute to cell cycle progression in gastric cancer. (PMID:28634044)
• miR218 functions as a tumor suppressor in human glioma. (PMID:29138857)
• findings showed that miR-218-2 rs11134527 and miR-301b rs384262 variant might contribute to increase the risk of breast cancer in a sample of Iranian population (PMID:29317318)
• Functional depletion of the alternative splicing factors Muscleblind-like (MBNL 1 and 2) is at the basis of the neuromuscular disease myotonic dystrophy type 1 (DM1). Here, we screen for miRNAs that regulate MBNL1 and MBNL2 in HeLa cells. We thus identify miR-23b and miR-218, and confirm that they downregulate MBNL proteins in this cell line. (PMID:29946070)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir218a-2	ENSDARG00000083023
mus_musculus	Mir218-2	ENSMUSG00000065583
rattus_norvegicus	Mir218-2	ENSRNOG00000035481

Paralogs (1): MIR218-1 (ENSG00000207732)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.