MIR27B

Summary

MIR27B (microRNA 27b, HGNC:31614) is a microRNA gene on chromosome 9q22.32.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407019 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31614
Approved symbol	MIR27B
Name	microRNA 27b
Location	9q22.32
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-27b, MIR-27b
Ensembl gene	ENSG00000207864
Ensembl biotype	miRNA
OMIM	610636
Entrez	407019
RNAcentral	URS000075B0A5 — miRNA, 97 nt, 16 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385129

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385129 — 1 exons

Exon	Start	End
ENSE00001500135	95085445	95085541

Expression profiles

Bgee: expression breadth ubiquitous, 119 present calls, max score 96.99.

Top tissues by expression

119 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right coronary artery	UBERON:0001625	96.99	gold quality
sural nerve	UBERON:0015488	95.99	gold quality
descending thoracic aorta	UBERON:0002345	95.60	gold quality
placenta	UBERON:0001987	94.09	gold quality
skeletal muscle tissue	UBERON:0001134	93.82	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	92.07	gold quality
endometrium	UBERON:0001295	91.88	gold quality
thoracic aorta	UBERON:0001515	89.91	gold quality
mucosa of stomach	UBERON:0001199	89.09	gold quality
body of pancreas	UBERON:0001150	89.01	gold quality
ascending aorta	UBERON:0001496	88.92	gold quality
left uterine tube	UBERON:0001303	88.59	gold quality
duodenum	UBERON:0002114	88.56	gold quality
esophagus mucosa	UBERON:0002469	86.92	gold quality
right adrenal gland	UBERON:0001233	86.85	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	86.79	gold quality
left coronary artery	UBERON:0001626	86.62	gold quality
esophagus	UBERON:0001043	86.37	gold quality
lower esophagus muscularis layer	UBERON:0035833	85.77	gold quality
lower esophagus	UBERON:0013473	85.73	gold quality
tibial artery	UBERON:0007610	85.32	gold quality
popliteal artery	UBERON:0002250	85.27	gold quality
right adrenal gland cortex	UBERON:0035827	85.24	gold quality
pancreas	UBERON:0001264	85.23	gold quality
uterus	UBERON:0000995	85.20	gold quality
calcaneal tendon	UBERON:0003701	85.20	gold quality
skin of abdomen	UBERON:0001416	85.17	gold quality
adrenal tissue	UBERON:0018303	85.17	gold quality
stomach	UBERON:0000945	85.16	gold quality
myometrium	UBERON:0001296	85.04	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.48

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• these results suggest that the anti-adipogenic effect of miR-27b in hMADS cells is due, at least in part, to suppression of PPARgamma. (PMID:19800867)
• miR-27b may play a role in regulating the expression of MMP-13 in human chondrocytes (PMID:20131257)
• inhibition of miR-27b, which was induced upon LPS exposure, partially reversed PPARgamma1 mRNA decay, whereas miR-27b overexpression decreased PPARgamma1 mRNA content (PMID:20164187)
• TNF-alpha-induced A(2B)AR expression in colonic epithelial cells is post-transcriptionally regulated by miR27b and miR128a (PMID:20388705)
• miR-2b is downregulated in melanoma cells exposed to a human embryonic stem cell environment. (PMID:20495621)
• serum levels of miR-130a, miR-27b and miR-210 may serve as potential biomarkers for early stage atherosclerosis obliterans (PMID:20888330)
• The overexpression and inhibition of miR-21 or miR-27b in HuH7 cells significantly decreased and increased the PPARalpha protein level, respectively, but not PPARalpha mRNA level. (PMID:21562928)
• results suggest that miR-200c and miR-27b downregulated by HGF might play an important role for EMT and ECM degradation with HGF autoactivation followed by enhanced invasive characteristics of squamous cell carcinoma of the head and neck (PMID:21899661)
• aberrantly up-regulated miR-27b may be one of the critical factors that contribute to malignancy in human gliomas (PMID:21922148)
• analyses revealed miR-27b was significantly down-regulated in OLP tissue; miR-27b expression was more suppressed in atrophic-erosive OLP than in reticular OLP. miR-27b was found to be expressed in epithelial keratinocyte layer of normal and OLP tissues. (PMID:22077423)
• in neuroblastoma, miR-27b functions as a tumor suppressor by inhibiting the tumor-promoting function of PPARgamma, which triggers an increased inflammatory response (PMID:22120719)
• Altered miR-23b/-27b expression and miR-221/222 expression may be associated with the castration resistant prostate cancer process. (PMID:22127852)
• Data show that miR-27a/b regulated the expression of the angiogenesis inhibitor semaphorin 6A (SEMA6A) in vitro and in vivo and targeted the 3’-untranslated region of SEMA6A. (PMID:22184411)
• High miR-27b is associated with melanoma. (PMID:22213330)
• in a human hepatocyte cell line (Huh7) that miR-27b regulates the expression (messenger RNA [mRNA] and protein) of several key lipid-metabolism genes, including Angptl3 and Gpam. (PMID:22777896)
• miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research. (PMID:22902387)
• Downregulation of microRNA -27b is associated with metastatic, castration-resistant prostate tumors. (PMID:23300597)
• miR-23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-alpha in breast cancer (PMID:23338610)
• An anti-tumor role for miR-27b and its novel target VEGFC in vivo could lead to tumor necrosis. (PMID:23593282)
• We aimed to evaluate the effects of sex, sex hormones, and polycystic ovary syndrome and their interactions with obesity on the expression in the circulation of these miRNAs. (PMID:24037889)
• we demonstrated the novel finding that ectopic expression of miR-27a or miR-27b impaired mitochondrial biogenesis (PMID:24133204)
• miR-27b rescues impaired bone marrrow derived angiogenic cell angiogenesis via TSP-1 suppression, semaphorin 6A expression, and p66shc-dependent mitochondrial oxidative stress and improves BMAC therapy in wound healing in type 2 diabetic mice. (PMID:24177325)
• DROSHA rs10719T>C polymorphism may be associated with bladder cancer risk in a Chinese population, and hsa-miR-27b can influence the expression of DROSHA protein by binding with 3’UTR (PMID:24312312)
• Our data provide new evidence that 6G-induced myeloid leukemia cell death is initiated by reactive oxygen species and mediated through an increase in miR-27b expression and DNA damage. (PMID:24378438)
• Our results demonstrated a tumor-suppressive role of miR-27b in NSCLC, suggesting a potential therapeutic target for NSCLC. (PMID:24390089)
• Taken together, these results suggest that miR-27a and miR-27b expression may be pharmacologically relevant modulators of Dihydropyrimidine dehydrogenase enzyme function in the liver. (PMID:24401318)
• These findings indicate that miR-27a/b affects the efflux, influx, esterification and hydrolysis of cellular cholesterol by regulating the expression of ABCA1, apoA1, LPL, CD36 and ACAT1. (PMID:24608080)
• Results demonstrated that miR-27b targets Gremlin 1, and that this regulation likely represents an important control point in fi brotic pathways. (PMID:24633904)
• we found that miR-27 suppressed growth and invasion of non-small cell lung cancer cells partially by targeting Sp1 (PMID:25012245)
• miR-23b and miR-27b function as tumor suppressors, targeting several oncogenic genes in clear cell renal cell carcinoma cells (PMID:25014580)
• downregulation of the miR-23b/27b/24-1 cluster was a frequent event in prostate cancer, and these clustered miRNAs functioned as tumor suppressors (PMID:25115396)
• For CYP1B1 regulation, IL6 downregulated the CYP1B1-targeting microRNA miR27b through a mechanism involving DNA methylation. (PMID:25333344)
• We conclude that the decreased expression of the tumor-suppressive miR-23b/27b cluster enhanced cancer cell proliferation, migration and invasion in BC through direct regulation of EGFR and c-Met signaling pathways (PMID:25405368)
• High miR-27b expression or low CRISP2 protein expression was significantly associated with low sperm progressive motility, abnormal morphology, and infertility. (PMID:25505194)
• miR-27b promotes drug response specifically in patients carrying p53-wild-type or CYP1B1-high signature (PMID:25698578)
• High serum expression levels of miR-27a and miR-27b correlate with poor response to chemotherapy in esophageal cancer patients.miR-27b is associated with chemoresistance in esophageal cancer through transformation of normal fibroblasts. (PMID:26026166)
• identified microRNA-27b (miR-27b) as a key regulator for the generation of a side-population in breast cancer cells that showed CSC properties (PMID:26065921)
• Findings suggest that down-regulation of miR98 and miR27b promotes CCL18-mediated invasion and migration of breast cancer cells. (PMID:26244871)
• Mir-27b is associated with diabetic retinopathy incidence and progression in type 1 diabetes patients. (PMID:26395742)
• the present study revealed that miR-27b is upregulated by HPV16 E7 to inhibit PPARg expression and promotes proliferation and invasion in cervical carcinoma cells. (PMID:26397063)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir27d	ENSDARG00000082462
mus_musculus	Mir27b	ENSMUSG00000065475

Paralogs (1): MIR27A (ENSG00000207808)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.