MIR299

Summary

MIR299 (microRNA 299, HGNC:31618) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407023 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31618
Approved symbol	MIR299
Name	microRNA 299
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-299
Ensembl gene	ENSG00000207749
Ensembl biotype	miRNA
Entrez	407023
RNAcentral	URS0000656761 — miRNA, 63 nt, 3 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385016

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385016 — 1 exons

Exon	Start	End
ENSE00001500023	101023794	101023856

Expression profiles

Bgee: expression breadth broad, 40 present calls, max score 95.82.

Top tissues by expression

40 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	95.82	gold quality
stomach	UBERON:0000945	85.58	gold quality
lung	UBERON:0002048	84.33	gold quality
colon	UBERON:0001155	80.92	gold quality
intestine	UBERON:0000160	80.58	gold quality
heart left ventricle	UBERON:0002084	75.34	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	75.01	gold quality
calcaneal tendon	UBERON:0003701	72.72	gold quality
islet of Langerhans	UBERON:0000006	72.49	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	71.25	gold quality
left adrenal gland cortex	UBERON:0035825	69.98	gold quality
left adrenal gland	UBERON:0001234	69.91	gold quality
body of pancreas	UBERON:0001150	68.79	gold quality
lower esophagus muscularis layer	UBERON:0035833	68.63	gold quality
omental fat pad	UBERON:0010414	68.57	gold quality
skin of leg	UBERON:0001511	68.46	gold quality
putamen	UBERON:0001874	67.20	gold quality
transverse colon	UBERON:0001157	67.05	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	66.92	gold quality
ascending aorta	UBERON:0001496	66.88	gold quality
liver	UBERON:0002107	66.83	gold quality
Brodmann (1909) area 9	UBERON:0013540	66.70	gold quality
body of stomach	UBERON:0001161	66.45	gold quality
nucleus accumbens	UBERON:0001882	65.84	gold quality
substantia nigra	UBERON:0002038	65.50	gold quality
Ammon’s horn	UBERON:0001954	64.82	gold quality
subcutaneous adipose tissue	UBERON:0002190	64.71	gold quality
skin of abdomen	UBERON:0001416	64.66	gold quality
cerebellar hemisphere	UBERON:0002245	64.55	gold quality
anterior cingulate cortex	UBERON:0009835	64.48	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.48

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 34)

• hsa-mir-299-5p targets OPN. (PMID:19538464)
• Hsa-miR-299-5p emerged as having a role in controlling CD34+ progenitor fate, grown in multilineage culture conditions. (PMID:21364636)
• Early insight into the role of hsa-miR-299-3p in the modulation of replicative senescence in human umbilical vein endothelial cells. (PMID:23362143)
• Mir-299-5p, mapped to the 14q32.31 locus, regulates proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. (PMID:24166498)
• MiR2993p has crucial roles in the progression of Hep2 cancer cells by interacting with hTERT mRNA. (PMID:25634687)
• miR-299-3p promotes the sensibility of lung cancer to doxorubicin through suppression of ABCE1, at least partly. Therefore, the disordered decreased of miR-299-3p and resulting ABCE1 up-expression may contribute to chemoresistance of lung cancer (PMID:26617714)
• Findings showed that during the alteration of normal tissue to cancerous one, the expression of miR-299-5p was downregulated in intestinal-type gastric adenocarcinoma. (PMID:27007598)
• Results show that MiR-299 expression was downregulated in glioblastoma tissues and cell lines and, was involved in the TUG1-regulated tumor-induced angiogenesis through inhibiting the expression of VEGFA by targeting its 3’-UTR. (PMID:27345398)
• lncRNA-TUG1 enhances the evolution and progression of EC through inhibiting miR-299 and miR-34a-5p. (PMID:28404901)
• Results provide the first evidence that ELL2 is a direct target of miR-299 and increased ELL2 expression and down-regulation of miR-299 are associated with GBM progression and poor prognosis in patients. (PMID:28531325)
• MicroRNA-299 functions as a suppressor in colon cancer cells by targeting VEGFA. (PMID:28738498)
• the results showed the up-regulation of miR-299 in the placental tissues from women with severe pre-eclampsia, and miR-299 suppressed the invasion and migration of trophoblast cells partly via targeting HDAC2. (PMID:29145147)
• MiR-299 is significantly upregulated in the ovarian cancers and suppression of its expression inhibits the proliferation by induction of apoptosis as well suppresses migration and invasion of the SKOV3 cancers cells. OCT-4 was found to be putative target of miR-99-3p in ovarian cancer and inhibition of OCT-4 had similar effects as that of miR-299 inhibition on cell migration and invasion. (PMID:29758200)
• miR-299-5p promotes cell cycle transition and proliferation in osteosarcoma cells by regulation the expression of cell cycle regulatory proteins. (PMID:29771409)
• miR-299-3p suppresses migration, invasion and proliferation of Hepatocellular carcinoma cells cells via directly targeting SIRT5 (PMID:30170358)
• MiR-299-5p knockdown suppressed the progression of HCC by targeting SIAH1, highlighting its role as a potential biomarker and therapeutic target of hepatocellular carcinoma. (PMID:30266288)
• miR-299-3p was significantly downregulated in thyroid cancer (TC) tissues and cell lines. miR-299-3p could inhibit cell proliferation and cell cycle progression, whereas remarkably promote cell apoptosis in TC cell lines. Bioinformatics predicted that SHOC2 might be a potential target of miR-299-3p. Subsequent Dual-Luciferase reporter analysis validated this hypothesis. (PMID:30657565)
• Bioinformatics and luciferase reporter assays identified heparanase (HPSE) as a direct target of miR2993p. (PMID:31257534)
• a novel mechanism of TUG1 in RCC tumorigenesis (PMID:31310753)
• Inhibition of TUG1/miRNA-299-3p Axis Represses Pancreatic Cancer Malignant Progression via Suppression of the Notch1 Pathway. (PMID:31655908)
• the miR-299 overexpression enhanced the chemosensitivity of the HK1 cells to 5-FU and also caused a decrease in their invasion ability. miR-299 may exhibit a therapeutic implication in Nasopharyngeal cancer (NC) may prove useful in NC treatment. (PMID:31786874)
• Periodontal ligament cells regulate osteogenesis via miR-299-5p in mesenchymal stem cells. (PMID:31951879)
• MicroRNA2995p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39. (PMID:32020227)
• LINC00467 enhances head and neck squamous cell carcinoma progression and the epithelial-mesenchymal transition process via miR-299-5p/ubiquitin specific protease-48 axis. (PMID:32159247)
• Multifaceted Function of MicroRNA-299-3p Fosters an Antitumor Environment Through Modulation of Androgen Receptor and VEGFA Signaling Pathways in Prostate Cancer. (PMID:32198489)
• MIR205HG acts as a ceRNA to expedite cell proliferation and progression in lung squamous cell carcinoma via targeting miR-299-3p/MAP3K2 axis. (PMID:32513149)
• LINC01128 regulates the development of osteosarcoma by sponging miR-299-3p to mediate MMP2 expression and activating Wnt/beta-catenin signalling pathway. (PMID:33108067)
• Silencing of long non-coding RNA HCP5 inhibits proliferation, invasion, migration, and promotes apoptosis via regulation of miR-299-3p/SMAD5 axis in gastric cancer cells. (PMID:33371778)
• Long non-coding RNA ZEB1-AS1 promotes proliferation and metastasis of hepatocellular carcinoma cells by targeting miR-299-3p/E2F1 axis. (PMID:33788950)
• N(6)-methyladenosine (m(6)A)-mediated lncRNA DLGAP1-AS1enhances breast canceradriamycin resistance through miR-299-3p/WTAP feedback loop. (PMID:34866525)
• Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis. (PMID:36260520)
• MicroRNA-299-3p inhibits cell proliferation, motility, invasion and angiogenesis via VEGFA in upper tract urothelial carcinoma. (PMID:38049938)
• DOK7, a target of miR-299-5p, suppresses the progression of bladder cancer. (PMID:38095644)
• Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1. (PMID:38394889)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir299a	ENSMUSG00000106653

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.