MIR300

Summary

MIR300 (microRNA 300, HGNC:33636) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126297 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33636
Approved symbol	MIR300
Name	microRNA 300
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-300
Ensembl gene	ENSG00000215957
Ensembl biotype	miRNA
Entrez	100126297
RNAcentral	URS000075D97A — miRNA, 83 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000401138

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000401138 — 1 exons

Exon	Start	End
ENSE00001546482	101041363	101041445

Expression profiles

Bgee: expression breadth broad, 57 present calls, max score 98.27.

Top tissues by expression

57 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	98.27	gold quality
lung	UBERON:0002048	84.80	gold quality
liver	UBERON:0002107	83.50	gold quality
stomach	UBERON:0000945	80.96	gold quality
placenta	UBERON:0001987	77.83	gold quality
kidney	UBERON:0002113	77.64	gold quality
blood	UBERON:0000178	74.82	gold quality
intestine	UBERON:0000160	73.47	gold quality
gastrocnemius	UBERON:0001388	73.37	gold quality
heart	UBERON:0000948	72.70	gold quality
gall bladder	UBERON:0002110	72.03	gold quality
adult mammalian kidney	UBERON:0000082	71.24	gold quality
endometrium	UBERON:0001295	68.98	gold quality
amygdala	UBERON:0001876	68.55	gold quality
right atrium auricular region	UBERON:0006631	68.35	gold quality
lower esophagus muscularis layer	UBERON:0035833	67.97	gold quality
left adrenal gland cortex	UBERON:0035825	67.71	gold quality
body of pancreas	UBERON:0001150	67.65	gold quality
colon	UBERON:0001155	67.43	gold quality
esophagus mucosa	UBERON:0002469	66.67	gold quality
body of stomach	UBERON:0001161	66.54	gold quality
hypothalamus	UBERON:0001898	66.35	gold quality
left adrenal gland	UBERON:0001234	66.23	gold quality
left ovary	UBERON:0002119	66.01	gold quality
thoracic aorta	UBERON:0001515	65.95	gold quality
subcutaneous adipose tissue	UBERON:0002190	65.91	gold quality
substantia nigra	UBERON:0002038	65.89	gold quality
skin of abdomen	UBERON:0001416	65.86	gold quality
vagina	UBERON:0000996	65.58	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	65.58	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.48

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 27)

• miR-300 can enhance the self-renewal of glioma stem-like cells and reduce differentiation. LZTS2 is a direct target of miR-300. (PMID:24464870)
• miR-300 inhibits epithelial to mesenchymal transition and metastasis by targeting Twist in human epithelial cancer. (PMID:24885626)
• Report feedback loop between PTTG1 targeting miRNAs,including mir300, PTTG1 and p53 that promotes pituitary tumorigenesis. (PMID:26320179)
• Data show that microRNA miR-300 directly represses vascular endothelial growth factor-C (VEGF-C) expression via binding to the 3’ untranslated regions (3’-UTR) of VEGF-C. (PMID:26824419)
• miR-300 promoted proliferation and epithelial-mesenchymal transition-mediated colorectal cancer cell migration and invasion by targeting p53. (PMID:27779716)
• LncRNA TUG1 promotes gallbladder carcinoma cell proliferation, metastasis and epithelial-mesenchymal transition progression by functioning as a miRNA sponge to abrogate the endogenous effect of miR-300. (PMID:28178615)
• MicroRNA-300 is a tumor suppressor microRNA in non-small cell lung cancer by downregulating HIF3alpha expression. (PMID:28485161)
• Twist1 was demonstrated to act as a direct target of miR300, and was negatively regulated by miR300. In addition, miR300 overexpression downregulated the expression of the primary factors involved in the NFkappaB signaling pathway. (PMID:28765882)
• miR-300 regulates the cellular sensitivity to ionizing radiation through targeting p53 and apaf1 in lung cancer cells. (PMID:28895780)
• we first identified that the level of miR-300 was significantly upregulated in the blood samples of acute lung injury patients compared with healthy control (PMID:29441890)
• Serum level of miR-300 and tumor necrosis factor-alpha (TNFalpha) was increased in the knee osteoarthritis (KOA) patients. (PMID:29739086)
• miR-300 targeting modulates FAK, and the PI3K/AKT signaling pathway inhibits the epithelial-mesenchymal transition and suppresses the migration and invasion of hepatocellular carcinoma cells. (PMID:29864952)
• Study shows that miR300 expression is increased in hepatocellular carcinoma (HCC) cell lines and tissues and associated with increased risk for invasiveness and relapse. Data revealed that miRNA300 functions as an oncogene in liver cancer by inhibiting FOXO1 and interacting with the AKT/mTOR signaling pathway. (PMID:30272296)
• miR-300 directly targeted SP1 and suppressed its expression by specifically binding to its 3’-untranslated region. Small interference RNAs silencing SP1 phenocopied the effects of miR-300 overexpression, while restoration of SP1 expression partially rescued the inhibition of metastasis induced by miR-300 overexpression. (PMID:30526300)
• Study revealed that miR300 expression was significantly decreased in hepatocellular carcinoma (HCC) cell lines compared with normal liver cells. LEF1 which was verified as a direct target gene of miR300, promoted cell proliferation, migration and invasion and mediates the effects of miR300. Low expression of miR300 and high expression of LEF1 in HCC tissues is associated with poor prognosis of patients with HCC. (PMID:30968150)
• LncRNA DLEU1/microRNA-300/RAB22A axis regulates migration and invasion of breast cancer cells. (PMID:31841195)
• MiRNA-300 suppresses proliferation, migration and invasion of non-small cell lung cancer via targeting ETS1. (PMID:31858551)
• LINC00472 promotes osteogenic differentiation and alleviates osteoporosis by sponging miR-300 to upregulate the expression of FGFR2. (PMID:32432728)
• Serum ROCK2, miR-300 and miR-450b-5p levels in two different clinical phenotypes of multiple sclerosis: Relation to patient disability and disease progression. (PMID:32781341)
• Persistence of Drug-Resistant Leukemic Stem Cells and Impaired NK Cell Immunity in CML Patients Depend on MIR300 Antiproliferative and PP2A-Activating Functions. (PMID:32974613)
• Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1. (PMID:33064976)
• UBE2C mRNA expression controlled by miR-300 and HuR determines its oncogenic role in gastric cancer. (PMID:33223052)
• [MiR-300 inhibits invasion and metastasis of osteosarcoma cell MG63 by negatively regulating PTTG1]. (PMID:33624604)
• MiR-300 promotes the proliferation, migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis by targeting IL-37. (PMID:35658770)
• Expression Levels of MicroRNA-300/BCL2L11 in Papillary Thyroid Cancer and Their Clinical Diagnostic Values. (PMID:37231813)
• Long-chain noncoding RNA LINC01569 upregulates filamin A-interacting protein 1-like to prevent metastasis of triple-negative breast cancer via sponging miR-300. (PMID:37955081)
• Has-miR-300-GADD45B promotes melanoma growth via cell cycle. (PMID:38070141)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.