MIR301B

Summary

MIR301B (microRNA 301b, HGNC:33667) is a microRNA gene on chromosome 22q11.21.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126318 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33667
Approved symbol	MIR301B
Name	microRNA 301b
Location	22q11.21
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-301b
Ensembl gene	ENSG00000212102
Ensembl biotype	miRNA
Entrez	100126318
RNAcentral	URS000075A590 — miRNA, 78 nt, 7 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000390813

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000390813 — 1 exons

Exon	Start	End
ENSE00001508490	21652981	21653058

Expression profiles

Bgee: expression breadth broad, 22 present calls, max score 75.33.

Top tissues by expression

22 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
heart	UBERON:0000948	75.33	gold quality
right atrium auricular region	UBERON:0006631	73.93	gold quality
monocyte	CL:0000576	71.71	gold quality
ascending aorta	UBERON:0001496	68.23	gold quality
transverse colon	UBERON:0001157	67.36	gold quality
endometrium	UBERON:0001295	67.31	gold quality
heart left ventricle	UBERON:0002084	66.77	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.36	gold quality
esophagus mucosa	UBERON:0002469	65.72	gold quality
body of pancreas	UBERON:0001150	65.67	gold quality
anterior cingulate cortex	UBERON:0009835	63.34	gold quality
pituitary gland	UBERON:0000007	61.96	gold quality
tibial nerve	UBERON:0001323	61.59	gold quality
hypothalamus	UBERON:0001898	61.24	gold quality
right hemisphere of cerebellum	UBERON:0014890	61.05	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	60.14	gold quality
spleen	UBERON:0002106	59.14	gold quality
colon	UBERON:0001155	56.35	gold quality
skin of leg	UBERON:0001511	52.88	gold quality
testis	UBERON:0000473	48.46	gold quality
left testis	UBERON:0004533	47.95	gold quality
right testis	UBERON:0004534	45.61	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.06

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 29)

• blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. (PMID:20470754)
• miR-301b may have potential as a novel therapeutic target for cancer treatment due to its stimulatory effects on cell invasiveness. (PMID:24398967)
• microRNA-130/301 controls vasoconstriction in pulmonary hypertension (PMID:25505270)
• MicroRNA301 might function as a potential diagnostic biomarker for HCC. (PMID:26191271)
• miR-301a/b-NDRG2 might be an important axis modulating autophagy and viability of prostate cancer cells under hypoxia. (PMID:26813459)
• High miR301b expression is associated with Pancreatic Cancer. (PMID:27197190)
• miR-301b is up-regulated in lung cancer. Hypoxia induces miR-301b expression in lung cancer. MicroRNA-301b enhances cell proliferation, reduces apoptosis and increases resistance to chemotherapy (PMID:27352910)
• The miR-130b301b cluster directs epigenetic activation of cell cycle inhibitors required for DNA damage response activation, thus stimulating the senescence-associated secretory phenotype. Overexpression of miR-130b301b cluster markedly reduced the malignant phenotype of prostate cancer cells. (PMID:28166834)
• Mechanistic investigation revealed a miR-301b~miR-130b-PPARgamma axis, whose expression pattern in umbilical cord (UC), adipose tissue (Ad) and bone marrow mesenchymal stem cells significantly correlates with their adipogenic capacity. (PMID:28442776)
• findings showed that miR-218-2 rs11134527 and miR-301b rs384262 variant might contribute to increase the risk of breast cancer in a sample of Iranian population (PMID:29317318)
• miR-301b plays an oncogenic role in triple negative breast cancer (TNBC) possibly by downregulating CYLD and subsequently activating NF-kappaB p65, and this may provide a novel therapeutic approach for TNBC. (PMID:30269739)
• miRNA-301b-3p accelerates migration and invasion of high-grade ovarian serous tumor via targeting CPEB3/EGFR axis. (PMID:30834603)
• miR-301b represses the proliferation and invasion of glioma cells by up-regulating GPC5 expression (PMID:30951720)
• MicroRNA-301b-3p contributes to tumour growth of human hepatocellular carcinoma by repressing vestigial like family member 4. (PMID:31207037)
• High miR301b expression is associated with proliferation, migration and invasion of prostate cancer. (PMID:31442444)
• miR-301-3p was highly expressed in gastric cancer contributed to tumor progression via attenuating ZBTB4 (PMID:31585814)
• Inhibiting microRNA-301b suppresses cell growth and targets RNF38 in cervical carcinoma. (PMID:32643846)
• Oncogenic microRNA-301b regulates tumor repressor dystrobrevin alpha to facilitate cell growth, invasion and migration in esophageal cancer. (PMID:32737801)
• SLC16A1-AS1 enhances radiosensitivity and represses cell proliferation and invasion by regulating the miR-301b-3p/CHD5 axis in hepatocellular carcinoma. (PMID:32748357)
• miR-301b and NR3C2 co-regulate cells malignant properties and have the potential to be independent prognostic factors in breast cancer. (PMID:33063403)
• Smoking load reduction is insufficient to downregulate miR-301b, a lung cancer promoter. (PMID:33273694)
• MIR-301b-3p Promotes Lung Adenocarcinoma Cell Proliferation, Migration and Invasion by Targeting DLC1. (PMID:33754907)
• LINC01089 suppresses lung adenocarcinoma cell proliferation and migration via miR-301b-3p/STARD13 axis. (PMID:34281560)
• MiR-301b-3p Promotes the Occurrence and Development of Breast Cancer Cells via Targeting HOXA5. (PMID:34369713)
• MicroRNA-301b-3p facilitates cell proliferation and migration in colorectal cancer by targeting HOXB1. (PMID:34488545)
• LINC01089, suppressed by YY1, inhibits lung cancer progression by targeting miR-301b-3p/HPDG axis. (PMID:34561789)
• CircARHGAP12 Triggers Mesenchymal Stromal Cell Autophagy to Facilitate its Effect on Repairing Diabetic Wounds by Sponging miR-301b-3p/ATG16L1 and miR-301b-3p/ULK2. (PMID:34933019)
• Overexpression Pattern of miR-301b in Osteosarcoma and Its Relevance with Osteosarcoma Cellular Behaviors via Modulating SNX10. (PMID:35732962)
• Expression profile and functional analysis of miR-301b in patients with breast cancer: A bioinformatics, biochemical, and histopathological study. (PMID:39173462)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir130b	ENSMUSG00000065572
mus_musculus	Mir301b	ENSMUSG00000076288
rattus_norvegicus	Mir130b	ENSRNOG00000035541
rattus_norvegicus	Mir301b	ENSRNOG00000040402

Paralogs (2): MIR301A (ENSG00000207996), MIR130A (ENSG00000208009)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.