MIR302A

Summary

MIR302A (microRNA 302a, HGNC:31623) is a microRNA gene on chromosome 4q25.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407028 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31623
Approved symbol	MIR302A
Name	microRNA 302a
Location	4q25
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-302, hsa-mir-302a
Ensembl gene	ENSG00000207927
Ensembl biotype	miRNA
OMIM	614596
Entrez	407028
RNAcentral	URS0000689179 — miRNA, 69 nt, 23 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385192

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385192 — 1 exons

Exon	Start	End
ENSE00001500198	112648183	112648251

Expression profiles

Bgee: expression breadth broad, 93 present calls, max score 85.86.

Top tissues by expression

93 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	85.86	gold quality
calcaneal tendon	UBERON:0003701	85.30	gold quality
duodenum	UBERON:0002114	85.14	gold quality
adult mammalian kidney	UBERON:0000082	84.97	gold quality
vermiform appendix	UBERON:0001154	83.68	gold quality
adrenal tissue	UBERON:0018303	83.53	gold quality
lymph node	UBERON:0000029	81.84	gold quality
bone marrow	UBERON:0002371	81.05	gold quality
blood	UBERON:0000178	79.35	gold quality
kidney	UBERON:0002113	78.85	gold quality
monocyte	CL:0000576	78.70	gold quality
endometrium	UBERON:0001295	77.99	gold quality
rectum	UBERON:0001052	76.08	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	72.92	gold quality
heart	UBERON:0000948	72.41	gold quality
heart left ventricle	UBERON:0002084	71.27	gold quality
islet of Langerhans	UBERON:0000006	71.11	gold quality
gastrocnemius	UBERON:0001388	70.49	gold quality
left uterine tube	UBERON:0001303	70.42	gold quality
right atrium auricular region	UBERON:0006631	69.97	gold quality
stomach	UBERON:0000945	69.93	gold quality
liver	UBERON:0002107	69.60	gold quality
body of pancreas	UBERON:0001150	69.34	gold quality
ectocervix	UBERON:0012249	69.24	gold quality
tibial artery	UBERON:0007610	68.97	gold quality
body of stomach	UBERON:0001161	68.87	gold quality
left coronary artery	UBERON:0001626	68.42	gold quality
intestine	UBERON:0000160	67.57	gold quality
left adrenal gland	UBERON:0001234	67.41	gold quality
left adrenal gland cortex	UBERON:0035825	67.38	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.59

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): NANOG, POU5F1, SOX2, ZFP42

Literature-anchored findings (GeneRIF, showing 40)

• The transcriptional activation of miR-302 and the translational repression of its targets, such as cyclin D1, may provide a link between Oct4/Sox2 and cell cycle regulation in pluripotent cells. (PMID:18710938)
• miR-302 appears as part of a stringent regulatory mechanism for p63 in germ cells, reminiscent of the tight control for p53 levels in somatic cells. (PMID:19342891)
• These findings suggest a crucial role for the miR-430/427/302 family in vertebrate embryogenesis by controlling germ layer specification. (PMID:19386261)
• Notch4 is one of the targets of miR-302a, which is upregulated in melanoma cells exposed to a human embryonic stem cell environment. (PMID:20495621)
• Data show that miR-302 simultaneously suppressed both the cyclin E-CDK2 and cyclin D-CDK4/6 pathways to block>70% of the G1-S cell cycle transition. (PMID:21062975)
• Findings suggest that Lefty is negatively modulated by miR-302s in hESCs, which plays an important role in maintaining the balance between pluripotency and germ layer specification. (PMID:21266536)
• two miRNA clusters, 106a-363 and 302-367, have roles in somatic cell reprogramming (PMID:21454525)
• Hsa-miR-302b and hsa-miR-372 regulate expression of a number of targets that influence reprogramming of human somatic cells. (PMID:21490602)
• Stable miR-302-367 cluster expression results in inhibition of CXCR4 leading to the disruption of the sonic hedgehog (SHH)-GLI-NANOG network.[miR 302] (PMID:21720384)
• miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors (PMID:22012620)
• Ascl2 knockdown results in tumor growth arrest by miRNA-302b-related inhibition of colon cancer progenitor cells (PMID:22384170)
• Data show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. (PMID:22511267)
• Generate miR-302s-induced pluripotent stem cells from HEK 293 cells via transfection of the miR-302s expression vector. (PMID:22515122)
• miR-302 is an ROS-sensitive regulator of ARID4a and CCL5 mRNAs. miR-302 plays a regulatory role during quiescence and proliferation. (PMID:22732186)
• Hyaluronan-CD44v3 interaction with Oct4-Sox2-Nanog promotes miR-302 expression leading to self-renewal, clonal formation, and cisplatin resistance in cancer stem cells from head and neck squamous cell carcinoma. (PMID:22847005)
• the negative regulatory loop of BMP4-miR-302-BMPRII is a potential mechanism for the maintenance and fine-tuning of the BMP4 signaling pathway in various systems (PMID:22988237)
• MiR-302 inhibits NR2F2 and promotes pluripotency through indirect positive regulation of OCT4. (PMID:23136034)
• A new cell cycle regulatory pathway was identified ain which the miR-302-367 cluster directly down-regulated both cyclin D1 and AKT1 and indirectly up-regulated p27Kip1 and p21Cip1, leading to the suppression of cervical cancer cell proliferation. (PMID:23185040)
• Hypoxia supports reprogramming of mesenchymal stromal cells via induction of embryonic stem cell-specific microRNA-302 cluster and pluripotency-associated genes. (PMID:23256541)
• MiR-302a-enhanced cisplatin sensitivity was partially mediated through the down-regulation of p21 in NT2 cells. (PMID:23625774)
• JMJD1C represses neural differentiation of hESCs at least partially by epigenetically sustaining miR-302 expression (PMID:24318875)
• we demonstrate knockdown of the miR-302/367 cluster by using the Kruppel-associated box repressor domain fused with specific transcription activator-like effectors (TALEs) designed to bind the miR-302/367 cluster promoter. (PMID:24319658)
• Our gene expression study revealed the overexpression of candidate genes after transduction of USSCs with miR-302-367 cluster (PMID:24705778)
• miR-302-related reprogramming improved drug sensitivity in hepatocellular carcinoma cells through AOF2 downregulation. (PMID:24740829)
• MiR-302a regulates proliferation and protects against oxidant-induced cell death mediated by the target genes CDKN1A and CCL5. (PMID:25144720)
• This work shows that miR-302 participation may facilitates the conversion of adult hepatocytes into pancreatic islets-like cells (PMID:25268319)
• In healthy tissues, the MIR302(high)/MIR203(low) asymmetry was characteristic of embryonic and induced pluripotency. In invasive ductal carcinoma, the MIR302/MIR203 asymmetry was associated with stem cell markers, metastasis, and shorter survival. (PMID:25306216)
• MiR-302b functions as a tumor suppressor in EOC by targeting RUNX1 and modulating the activity of the STAT3 signaling pathway. (PMID:25562167)
• MicroRNA-302/367 cluster governs hESC self-renewal by dually regulating cell cycle and apoptosis pathways (PMID:25801506)
• MicroRNA-302a suppresses tumor cell proliferation by inhibiting AKT in prostate cancer. (PMID:25922934)
• these data uncover a direct regulatory relationship between miR-302 and the Brg1 chromatin remodeling complex that controls gene expression and cell fate decisions in hESCs and suggests that similar mechanisms are at play during early human development (PMID:26119756)
• our results indicated that miR-302a overexpression was shown to inhibit proliferation and invasion of colorectal cancer cells (PMID:26191138)
• miR-302a plays a key role in inhibition of ovarian cancer cell proliferation, and enhancing apoptosis by targeting SDC1. (PMID:26191180)
• miR-302b inhibits SMMC-7721 cell invasion and metastasis by targeting AKT2 (PMID:26254095)
• Clustering expression levels of miR-302 validated target genes showed a significant correlation between miR-302/367 cluster miRNAs and a subset of validated gene targets in healthy and adjacent tumor tissues. (PMID:26363379)
• Data suggest microRNA302c, but not microRNA520e, promotes replication of influenza A virus H3N2 although the two microRNAs target same site of NFkappaB-inducing kinase (MAP3K14) 3prime untranslated region; studies were conducted in lung epithelial cells. (PMID:26602079)
• Results demonstrated that miR-302 is able to inhibit Abeta-induced cytotoxicity via activating Akt signaling to upregulate Nrf2 and Nanog expressions, leading to a marked restoration of insulin signaling in Alzheimer’s disease neurons (PMID:26890744)
• Our system uniquely provides sensitive detection of pluripotent stem cells and partially differentiated cells. In addition to its ability to eliminate undifferentiated cells, miR-302a switch also holds great potential in investigating the dynamics of differentiation and/or reprograming of live-cells based on intracellular information (PMID:27608814)
• MiR-302 is a pleiotropically acting miRNA family which may have significant implications in controlling cancer progression and invasion (PMID:27840154)
• miR302a targeted IGF1R and enhanced 5FUinduced cell death and viability inhibition in human colon cancer cells. Targeting miR302a may offer new therapeutic interventions in Colorectal cancer. (PMID:27840990)

Cross-species orthologs

0 orthologs

Paralogs (3): MIR302C (ENSG00000199102), MIR302D (ENSG00000199145), MIR302B (ENSG00000284463)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.