MIR302B

Summary

MIR302B (microRNA 302b, HGNC:31763) is a microRNA gene on chromosome 4q25.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442894 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31763
Approved symbol	MIR302B
Name	microRNA 302b
Location	4q25
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-302b
Ensembl gene	ENSG00000284463
Ensembl biotype	miRNA
OMIM	614597
Entrez	442894
RNAcentral	URS0000759FEB — miRNA, 73 nt, 6 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362188

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362188 — 1 exons

Exon	Start	End
ENSE00001436951	112648485	112648557

Expression profiles

Bgee: expression breadth broad, 79 present calls, max score 76.81.

Top tissues by expression

79 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
kidney	UBERON:0002113	76.81	gold quality
blood	UBERON:0000178	76.66	gold quality
rectum	UBERON:0001052	76.47	gold quality
endometrium	UBERON:0001295	76.26	gold quality
adrenal tissue	UBERON:0018303	75.73	gold quality
bone marrow	UBERON:0002371	75.12	gold quality
monocyte	CL:0000576	73.73	gold quality
calcaneal tendon	UBERON:0003701	73.67	gold quality
lymph node	UBERON:0000029	73.34	gold quality
gall bladder	UBERON:0002110	73.22	gold quality
left adrenal gland cortex	UBERON:0035825	72.26	gold quality
heart left ventricle	UBERON:0002084	70.83	gold quality
gastrocnemius	UBERON:0001388	70.71	gold quality
right adrenal gland	UBERON:0001233	70.61	gold quality
heart	UBERON:0000948	70.54	gold quality
smooth muscle tissue	UBERON:0001135	70.50	gold quality
right atrium auricular region	UBERON:0006631	69.97	gold quality
tibial artery	UBERON:0007610	69.71	gold quality
right frontal lobe	UBERON:0002810	68.20	gold quality
body of pancreas	UBERON:0001150	68.05	gold quality
fundus of stomach	UBERON:0001160	68.05	gold quality
body of stomach	UBERON:0001161	67.94	gold quality
stomach	UBERON:0000945	67.82	gold quality
subcutaneous adipose tissue	UBERON:0002190	67.10	gold quality
left adrenal gland	UBERON:0001234	66.90	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	66.29	gold quality
lower esophagus	UBERON:0013473	65.60	gold quality
ascending aorta	UBERON:0001496	65.59	gold quality
lower esophagus muscularis layer	UBERON:0035833	65.55	gold quality
esophagus	UBERON:0001043	65.51	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.29

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 33)

• These results suggest that miR-302b plays an important role in maintaining the pluripotency of embryonal carcinoma cells and probably embryonic stem cells , by post-transcriptional regulation of Cyclin D2 expression. (PMID:18930031)
• ethanol-induced neuronal apoptosis follows both the mitochondria-mediated (miR-497- and BCL2-mediated) and non-mitochondria-mediated (miR-302b- and CCND2-mediated) pathway. (PMID:21878650)
• Data show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. (PMID:22511267)
• Hsa-miR-302b acts as a “chemosensitizer” in human ovarian carcinoma cells and may represent a biomarker able to predict response to cisplatin treatment. (PMID:23484053)
• MiR-302b is a potential molecular marker of esophageal squamous cell carcinoma and functions as a tumor suppressor by post-transcriptionally regulating ErbB4. (PMID:24438167)
• MiR-302b regulates proliferation and protects against oxidant-induced cell death mediated by the target genes CDKN1A and CCL5. (PMID:25144720)
• Findings indicate that miR-302b plays a relevant role in breast cancer cell response to cisplatin through the modulation of the E2F1 transcription factor (E2F1)/ataxia telangiectasia mutated protein (ATM) axis. (PMID:26623722)
• Findings suggest that miR-302 inhibits BCRP expression via targeting the 3’-UTR of BCRP mRNA. miR-302 members may cooperatively downregulate BCRP expression to increase chemosensitivity of breast cancer cells. (PMID:26644266)
• The upregulation of miR-302b reduced the expression of CDK2, and inhibited ERK signaling pathway, thereby inhibiting cell proliferation and G1/S phase conversion rate. (PMID:27465546)
• We verified that NFIA binds to the IGFBP2 promoter and transcriptionally enhances IGFBP2 expression levels. We identified that NFIA-mediated IGFBP2 signaling pathways are involved in miR-302b-induced glioma cell death. (PMID:28323865)
• These finding suggests that miR-302b inhibits key transcription factors and cytokines by targeting ERBB4, IRF2 and CXCR4, implicating its role in the inhibition of CRI in EC. (PMID:28467773)
• MicroRNA-302b-3p suppresses cell proliferation through AKT pathway by targeting IGF-1R in human gastric cancer. (PMID:28743112)
• the aim of the present study was to compare the plasma levels of miR-302b between patients following acute myocardial infarction and healthy subjects. (PMID:28752786)
• our results indicate that miR-302b functions as a tumour repressor in the invasion and migration of osteosarcoma by directly downregulating Runx2 expression and may be a potential therapeutic target for osteosarcoma. (PMID:29042587)
• Low miR302b expression is associated with epithelial-mesenchymal transition in gastric cancer. (PMID:29273006)
• Data show that interleukin 1 receptor associated kinase 4 (IRAK4) and EPH receptor A2 (EphA2) were the functional targets of miR-302b. (PMID:29482609)
• Study showed that miR-302 microRNA cluster genes are involved in in vitro dedifferentiation of human pancreatic islet cells and inhibits the expression of multiple genes involved in the maintenance of beta-cell mature phenotype. (PMID:29649109)
• The abrogation of DDX6 expression inhibited iPSC generation, which was mediated by RNA decay targeting parental mRNAs supporting mesenchymal phenotypes, along with microRNAs, such as miR-302b-3p (PMID:30273347)
• The overexpression of miR-302b-3p downregulated the protein levels of GCNT3, N-cadherin, vimentin, and p-Erk and elevated the levels of E-cadherin. (PMID:30355927)
• Our findings indicate that miR-302b-5p might suppress hepatocellular carcinoma progression (PMID:31103408)
• miRNA-302s may act as oncogenes in human testicular germ cell tumours. (PMID:31235829)
• The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. (PMID:31767682)
• MiR-302b-5p enhances the neuroprotective effect of IGF-1 in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s disease by regulating inducible nitric-oxide synthase. (PMID:32474958)
• Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1alpha Activation. (PMID:32630449)
• LncUBE2R2-AS1 acts as a microRNA sponge of miR-302b to promote HCC progression via activation EGFR-PI3K-AKT signaling pathway. (PMID:32835579)
• Circular RNA circ_HN1 facilitates gastric cancer progression through modulation of the miR-302b-3p/ROCK2 axis. (PMID:32949310)
• Silencing of miR-302b-3p alleviates isoflurane-induced neuronal injury by regulating PTEN expression and AKT pathway. (PMID:33370590)
• MiR-302b Suppresses Tumor Metastasis by Targeting Frizzled 6 in OSCC. (PMID:33478325)
• SOX-17 is involved in invasion and apoptosis of colorectal cancer cells through regulating miR-302b-3p expression. (PMID:33739578)
• Low expression of lncRNA SBF2-AS1 regulates the miR-302b-3p/TGFBR2 axis, promoting metastasis in laryngeal cancer. (PMID:34644425)
• Analysis of the subcellular location of FAM230B and its interaction with premature miR-302b in osteosarcoma. (PMID:35639175)
• LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p. (PMID:37226251)
• Hypoxia enhances anti-fibrotic properties of extracellular vesicles derived from hiPSCs via the miR302b-3p/TGFbeta/SMAD2 axis. (PMID:37904135)

Cross-species orthologs

0 orthologs

Paralogs (3): MIR302C (ENSG00000199102), MIR302D (ENSG00000199145), MIR302A (ENSG00000207927)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.