MIR30B

Summary

MIR30B (microRNA 30b, HGNC:31625) is a microRNA gene on chromosome 8q24.22.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407030 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31625
Approved symbol	MIR30B
Name	microRNA 30b
Location	8q24.22
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-30b
Ensembl gene	ENSG00000207582
Ensembl biotype	miRNA
OMIM	619018
Entrez	407030
RNAcentral	URS00003426E6 — miRNA, 88 nt, 14 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384850

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384850 — 1 exons

Exon	Start	End
ENSE00001499857	134800520	134800607

Expression profiles

Bgee: expression breadth broad, 63 present calls, max score 86.94.

Top tissues by expression

63 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	86.94	gold quality
placenta	UBERON:0001987	82.38	gold quality
bone marrow	UBERON:0002371	79.78	gold quality
kidney	UBERON:0002113	79.45	gold quality
adrenal tissue	UBERON:0018303	79.11	gold quality
liver	UBERON:0002107	77.93	gold quality
blood	UBERON:0000178	76.97	gold quality
muscle of leg	UBERON:0001383	75.99	gold quality
heart	UBERON:0000948	75.96	gold quality
endometrium	UBERON:0001295	74.78	gold quality
monocyte	CL:0000576	74.43	gold quality
body of pancreas	UBERON:0001150	72.27	gold quality
stomach	UBERON:0000945	72.26	gold quality
adult mammalian kidney	UBERON:0000082	71.75	gold quality
omental fat pad	UBERON:0010414	70.96	gold quality
gastrocnemius	UBERON:0001388	70.93	gold quality
lung	UBERON:0002048	70.77	gold quality
right atrium auricular region	UBERON:0006631	70.76	gold quality
body of stomach	UBERON:0001161	69.12	gold quality
intestine	UBERON:0000160	68.87	gold quality
transverse colon	UBERON:0001157	67.98	gold quality
colon	UBERON:0001155	67.88	gold quality
spleen	UBERON:0002106	67.70	gold quality
esophagus mucosa	UBERON:0002469	67.70	gold quality
cerebellar hemisphere	UBERON:0002245	67.69	gold quality
popliteal artery	UBERON:0002250	67.50	gold quality
tibial artery	UBERON:0007610	67.50	gold quality
heart left ventricle	UBERON:0002084	66.31	gold quality
small intestine	UBERON:0002108	66.27	gold quality
tibial nerve	UBERON:0001323	66.17	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.85

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• Ectopic expression of miR-30b/30d promoted the metastatic behavior of melanoma cells by directly targeting the GalNAc transferase GALNT7. (PMID:21741600)
• These results suggest that compromise of autophagy by MIR30B allows intracellular H. pylori to evade autophagic clearance, thereby contributing to the persistence of H. pylori infections. (PMID:22647547)
• The post-transcriptional control of gene expression via miRNA modulation regulates human catalase. (PMID:22880027)
• Both miR-30b (and miR-30d) were significantly upregulated in receptive endometrium from fertile women. (PMID:22902743)
• Data indicate that miR-30b/c and miR-21 target respectively the 3’ untranslated region of caspase-3 and TAp63 mRNAs. (PMID:22964638)
• Respiratory syncytial virus upregulates human let-7 and mir-30b microRNAs by using mechanisms involving beta interferon and NF-kappaB. (PMID:23249809)
• Down-regulation of miR-30b might be involved in the pathogenesis of systemic sclerosis through its regulation of the expression of PDGF receptor beta. (PMID:23893664)
• Demonstrate role of miRNA-30b in calcific aortic valve disease as a regulator of human aortic valvular calcification and apoptosis through direct targeting of Runx2, Smad1, and caspase-3. (PMID:23968872)
• ADAR1 regulates the expression of ARHGAP26 through A-to-I RNA editing by disrupting the binding of miR-30b-3p and miR-573 within the 3’ UTR of ARHGAP26. (PMID:24067935)
• Hsa-miR-30b and hsa-miR-30c are negative regulators of cell death induced by loss of attachment through down-regulation of caspase 3 expression. (PMID:24129493)
• MYBL2 overexpression was associated with lower expression of miR-30a (P=0.024), miR-30b (P=0.021) and miR-30c (P=0.009). (PMID:24199710)
• Study reveals functional and mechanistic links between miRNA-30b and oncogene KRAS, PIK3CD and BCL2 in the pathogenesis of colorectal carcinoma. (PMID:24293274)
• MicroRNA-30b mediates metastasis in colorectal cells via homeobox protein SIX1. (PMID:24593661)
• miR-30b-5p is significantly diminished in gastric cancer tissues, providing the first insight into the epigenetic mechanism of miR-30b-5p down-regulation, induced by DNMT1, and the role of miR-30b-5p in gastric cancer carcinogenesis. (PMID:24913034)
• Data suggest that miR-30b may function as a novel tumor suppressor gene in gastric cancer by targeting plasminogen activator inhibitor-1 (PAI-1) and regulating the apoptosis of cancer cells. (PMID:25170877)
• overexpression of miR-103a-3p, miR-30b-5p and miR-29a-3p in L-Dopa treated patients with Parkinson disease (PD) was observed; promising candidate target genes for these were revealed by in silico analysis; data provide a rationale for clarifying role of the miRNAs in the pathogenesis of PD (PMID:25596505)
• these results demonstrate that miR-24, miR-30b, and miR-142-3p regulate phagocytosis and associated cytokine production in myeloid inflammatory cells through modulation of various genes involved in the pathway. (PMID:25601927)
• study found there was a greater expression rate of miR-200c, miR-141 and miR-30b in bladder cancer tissues than healthy adjacent control tissue (PMID:25703910)
• Regulation of miR-24, miR-30b, and miR-142-3p during macrophage and dendritic cell differentiation potentiates innate immunity. (PMID:25990241)
• overexpression of miR-30b can improve the Hcy-induced apoptosis in HCAECs by downregulating caspase-3 expression. Therefore, miR-30b may play an important role in Hcy-induced apoptosis in endothelial cells (PMID:26263983)
• MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 expression in gastric cancer cells. (PMID:26309359)
• reduces proinsulin expression and insulin content of beta-cells by directly targeting the transcriptional factor Neurod1 (PMID:26367486)
• miR-30b was involved in the process of osteoarthritis, and it probably functioned through its target gene ERG (PMID:26653555)
• we unravels a new miRs-based mechanism that helps maintain intracellular proteostasis and promote cell survival during ER stress through upregulation of miR-30b-5p and miR-30c-5p which target eIF2alpha and thereby inhibit the p-eIF2alpha/ATF4/CHOP pro-apoptotic pathway, identifying miR-30b-5p and miR-30c-5p as potentially new targets for anti-cancer therapies. (PMID:26898246)
• In summary, we identified HNF1A-AS1-miR-30b axis as a key regulator in hepatocarcinogenesis, which may be promising biomarkers and therapeutic targets in the future. (PMID:27084450)
• the significance of miR-30b downregulation in hepatocellular tumor metastasis and invasiveness, is reported. (PMID:27333771)
• Circulating hsa-let-7b and hsa-miR-30b might be associated with tuberculosis development by regulating the target genes involved in TLR-NF-kB mediated signal pathway. (PMID:27450007)
• Expression of miR-30b in coronary atherosclerosis samples, identified the effects of miR-30b on cell cycle and apoptosis. (PMID:27464494)
• Overexpression of miR-24, miR-30b and miR-142-3p attenuates uptake and processing of soluble antigen ovalbumin (Ova) in primary human macrophages and dendritic cells. Analysis of molecules regulating antigen presenting cells and T-cell receptor interaction shows miRNA-mediated induced expression of Programmed Death-Ligand 1 (PD-L1) which inhibits T-cell proliferation. (PMID:27611009)
• Droplet digital RT-PCR quantification of miR-30c-5p and miR-30d-5p revealed significantly reduced levels in metastatic castration resistant PCa (CRPC), when compared to healthy prostate tissues. (PMID:27683042)
• the molecular mechanism of trastuzumab action in BT474 cell line may be regulated by miR-26a and miR-30b and CCNE2 overexpression might play an important role in acquired trastuzumab resistance in HER2+ breast cancer. (PMID:28120942)
• 6 microRNAs (miRNAs) hsa-mir-483-5p, hsa-mir-486-5p, hsa-30b-5p, hsa-mir-200a-3p, hsa-mir-502-3p and hsa-mir-142-3p are selected as most promising plasma biomarker candidates for the detection of early Alzheimer’s disease (AD). (PMID:28179587)
• Data indicate that homeobox A1 (HOXA1) was a direct microRNA miR-30b target in esophageal cancer (EC) cells. (PMID:28189678)
• miR30b was upregulated in renal cell carcinoma (RCC) tissues from affected cell lines when compared with adjacent normal tissues and a normal kidney cell line, which is different to the downregulation of miR30b as observed in other types of cancer. miR30b is associated with RCC cell proliferation, invasion, migration and apoptosis, which indicated that miR30b acts as an oncogene in RCC. (PMID:28259953)
• This study showed that miR-30b-5p repressed cell proliferation and cell cycle of HCC cell lines and that miR-30b-5p mediated DNMT3A to repress proliferation, meanwhile it targeted USP37 for decelerating cell cycle. (PMID:28273636)
• MiR-30b-5p regulates GCN13, suppresses cell proliferation, metastasis and epithelial-to-mesenchymal transition in renal cell carcinoma. (PMID:28536082)
• miR-30b promotes glioblastoma cell proliferation, migration, and invasion via targeting PRRT2. (PMID:28550683)
• these results suggest that miR-30b plays important roles in kynurenine-induced increase of FOXO3 expression (PMID:28905195)
• results support that the regulation of miR-30b by VEGF in HUVEC is important for capillary morphogenesis, as increased miR-30b expression inhibits capillary morphogenesis through enhanced expression of TGFbeta2 (PMID:28977001)
• miR-30b may contribute to the development of alopecia areata. (PMID:29080678)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-mir-30c	ENSDARG00000080952
danio_rerio	dre-mir-30b	ENSDARG00000083528
mus_musculus	Mir30b	ENSMUSG00000065476

Paralogs (5): MIR30E (ENSG00000198974), MIR30C2 (ENSG00000199094), MIR30D (ENSG00000199153), MIR30A (ENSG00000207827), MIR30C1 (ENSG00000207962)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.