MIR30D

Summary

MIR30D (microRNA 30d, HGNC:31628) is a microRNA gene on chromosome 8q24.22.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407033 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31628
Approved symbol	MIR30D
Name	microRNA 30d
Location	8q24.22
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-30d
Ensembl gene	ENSG00000199153
Ensembl biotype	miRNA
OMIM	619019
Entrez	407033
RNAcentral	URS000036BDAF — miRNA, 70 nt, 18 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362283

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362283 — 1 exons

Exon	Start	End
ENSE00001437046	134804876	134804945

Expression profiles

Bgee: expression breadth broad, 49 present calls, max score 93.02.

Top tissues by expression

49 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal glomerulus	UBERON:0000074	93.02	gold quality
pituitary gland	UBERON:0000007	89.12	gold quality
sural nerve	UBERON:0015488	88.10	gold quality
calcaneal tendon	UBERON:0003701	85.44	gold quality
blood	UBERON:0000178	81.64	gold quality
adult mammalian kidney	UBERON:0000082	80.44	gold quality
heart	UBERON:0000948	80.41	gold quality
right atrium auricular region	UBERON:0006631	78.85	gold quality
lung	UBERON:0002048	78.01	gold quality
adrenal tissue	UBERON:0018303	77.15	gold quality
endometrium	UBERON:0001295	77.07	gold quality
placenta	UBERON:0001987	75.04	gold quality
gastrocnemius	UBERON:0001388	74.07	gold quality
stomach	UBERON:0000945	73.84	gold quality
body of stomach	UBERON:0001161	71.69	gold quality
thyroid gland	UBERON:0002046	71.03	gold quality
right frontal lobe	UBERON:0002810	70.69	gold quality
frontal lobe	UBERON:0016525	70.68	gold quality
uterus	UBERON:0000995	70.13	gold quality
left adrenal gland cortex	UBERON:0035825	69.64	gold quality
midbrain	UBERON:0001891	69.55	gold quality
omental fat pad	UBERON:0010414	68.78	gold quality
tibial artery	UBERON:0007610	68.45	gold quality
tibial nerve	UBERON:0001323	68.40	gold quality
urinary bladder	UBERON:0001255	68.08	gold quality
intestine	UBERON:0000160	67.73	gold quality
skin of leg	UBERON:0001511	67.69	gold quality
fundus of stomach	UBERON:0001160	67.16	gold quality
cerebellar hemisphere	UBERON:0002245	66.79	gold quality
cerebellum	UBERON:0002037	66.78	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.98

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• In addition to miR-125b and miR-504, the human TP53 gene is negatively regulated by two more miRNAs: miR-25 and miR-30d. (PMID:20935678)
• Ectopic expression of miR-30b/30d promoted the metastatic behavior of melanoma cells by directly targeting the GalNAc transferase GALNT7. (PMID:21741600)
• Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3 (PMID:22058146)
• our results suggest that miR-21, miR-205, miR-30d, and miR-24 may serve as potential novel non-invasive biomarkers for diagnosis of lung cancer (PMID:22782668)
• In fertile women in receptive endometrium miR-30d (and miR-30b) are upregulated. (PMID:22902743)
• miR-30d, miR-181a, & miR-199a-5p are down-regulated in several cancers & tumor cell lines. They act cooperatively to down-regulate GRP78 and induce apoptosis by directly targeting its 3’ untranslated region. (PMID:23085757)
• Authors identified miR-30d as a downregulator of SOCS1 expression by directly binding to 3’-UTR of SOCS1 in prostate cancer. (PMID:23231923)
• microRNA-10a and microRNA-30d have roles in kidney injury (PMID:23272089)
• Taken together, these results provide evidence that the oncomir mir-30d impairs the autophagy process by targeting multiple genes in the autophagy pathway. (PMID:23274497)
• The miR-30d post-transcriptionally suppresses expression of the oncoprotein metadherin (MTDH) by destabilizing its mRNA. (PMID:23416459)
• we report that the EZH2-miR-30d-KPNB1 signalling pathway is critical for malignant peripheral nerve sheath tumour cell survival in vitro and tumourigenicity in vivo (PMID:24132643)
• miR-30d negatively regulates the expression of key autophagy gene beclin 1, leading to suppression of cisplatin-activated autophagic response that protects thyroid carcinoma cells from apoptosis. (PMID:24345332)
• The overexpression of miR-30d or 181a suppressed HOXA11 mRNA. (PMID:25630974)
• MiR-221 and miR-30d repress prostate cancer cell proliferation, and this effect is partially rescued by Bmi-1 overexpression. (PMID:25761682)
• Results suggest that the miR-30d-5p/CCNE2 axis may contribute to NSCLC cell proliferation and motility. (PMID:25843294)
• Baseline plasma miR-30d level is associated with response to cardiac resynchronization therapy in heart failure. (PMID:25995320)
• Hsa-miR-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome. (PMID:26395145)
• These findings pinpoint that TGF-beta represses miR-30d through a Smad2/3-HDAC3-NCoR repression complex and provide novel insights into a potential target for the treatment of podocyte injury-associated glomerulopathies. (PMID:26432290)
• miR-30d directly bound to the 3’ untranslated region of Snail to suppress its expression and consequently inhibited the epithelial-mesenchymal transition process. (PMID:26501435)
• Although the mechanism is not clear, miR-124a and miR-30d potentially may be used as therapeutic targets and prognostic markers for breast cancer patients with type 2 diabetes mellitus . (PMID:26897751)
• Mir-30d increases intracellular survival of H. pylori in gastric epithelial cells through inhibition of multiple core proteins in the autophagy pathway. (PMID:27099441)
• Data report the epigenetic regulation of TET3 on miR-30d in TGF-beta1-induced epithelial-mesenchymal transition in ovarian cancer cells. (PMID:27141829)
• Kaplan-Meier survival analysis indicated that miR-125b-5p levels were associated with 6 month cardiovascular events in patients with AMI, but not miR30d5p. miR-125b-5p and miR-30d-5p presented a diagnostic value for early diagnosis of AMI, and miR30d5p may have a higher diagnostic value than cTnI (PMID:27176713)
• The receptive-up-regulated micro-RNA, mir-30d was also down-regulated in endometrial cells by polychlorinated biphenyls exposure. (PMID:27481218)
• These data indicate that HO-1 significantly suppresses HCC progression by regulating the miR-30d/miR-107 level, suggesting miR-30d/miR-107 regulation as a new molecular mechanism of HO-1 anticancer effect. (PMID:27571925)
• Low miR30d expression is associated with esophageal squamous cell carcinoma. (PMID:28184915)
• Our data highlight the complexity and tumour heterogeneity caused by hypoxia. MiR-210 as a hypoxic biomarker, and the targeting of miR-21 and miR-30d and/or the amino acid metabolism pathways may offer translational opportunities. (PMID:28207045)
• Kaplan-Meier survival analysis confirmed that patients with higher serum miR-30d levels had significantly lower mortality. (PMID:28214846)
• Studied copy number and amplification of miRNA miR-30d in disease progression of cervical squamous cell carcinomas. (PMID:28356144)
• Liver receptor homologue1 (LRH1) is a direct target of miR30d in colorectal carcinoma cells. (PMID:28440426)
• MiR-30d inhibited cell autophagy by directly targeting messenger RNA of ATG5, phosphoinositide 3-kinase, and Beclin1 and promoted cell apoptosis of human colon cancer cells. It is helpful to clarify the function of miR-30d in tumorigenesis of human cancers. (PMID:28651493)
• Studied role of miR-30d in tumor invasiveness and migration in non-small lung cancer (NSCLC). Found miR-30d suppressed cell migration and invasion by directly targeting nuclear factor I B (NFIB) in NSCLC. (PMID:28861760)
• miR-30b/d was the most significant microRNA for the follow-up analyses, which also showed lower expression in the hair follicle of alopecia areata patients (PMID:29080678)
• Data show that the 3’-untranslated region (3’-UTR) of LDHA mRNA was the direct target of microRNA-30d-5p (miR-30d-5p), which was low expressed in gallbladder cancer (GBC) tissues and associated with poor prognosis of GBC patients. (PMID:29569755)
• In this study, both ARID4A and ARID4B were identified as the target genes of miR-30d. (PMID:29797600)
• The expression of miR-30d-5p can significantly inhibit both Beclin-1 and Atg5 expression by targeting the 3’UTR of Beclin-1 and Atg5 at the mRNA level. Suppression of autophagy can transform microglial/macrophage polarization from M1 to M2 under ischemic conditions. (PMID:29807362)
• Low expression of miR-30D is associated with Prostate Cancer. (PMID:29916747)
• microRNA-30d mediated breast cancer invasion, migration, and epithelial-mesenchymal transition by targeting KLF11 and activating STAT3 pathway. (PMID:29923255)
• miR-30d may improve TGF-beta1-induced pulmonary fibrosis through direct binding to the 3’UTR of JAG1 and blocking JAG1/Notch signaling (PMID:30029934)
• MiR-30d inhibited the activation of the downstream PI3K/Akt signaling pathway by directly binding to its target gene, MTDH, which suggested that miR-30d plays an important anti-carcinogenic role in the pathological process of myltiple myeloma by targeting the MTDH/PI3K/Akt signaling pathway. (PMID:30132507)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir30d	ENSMUSG00000065437

Paralogs (5): MIR30E (ENSG00000198974), MIR30C2 (ENSG00000199094), MIR30B (ENSG00000207582), MIR30A (ENSG00000207827), MIR30C1 (ENSG00000207962)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.