MIR32

Summary

MIR32 (microRNA 32, HGNC:31631) is a microRNA gene on chromosome 9q31.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407036 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31631
Approved symbol	MIR32
Name	microRNA 32
Location	9q31.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-32
Ensembl gene	ENSG00000207698
Ensembl biotype	miRNA
OMIM	609355
Entrez	407036
RNAcentral	URS0000472EB8 — miRNA, 70 nt, 49 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384965

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384965 — 1 exons

Exon	Start	End
ENSE00001499972	109046229	109046298

Expression profiles

Bgee: expression breadth broad, 87 present calls, max score 83.81.

Top tissues by expression

87 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	83.81	gold quality
vermiform appendix	UBERON:0001154	83.24	gold quality
calcaneal tendon	UBERON:0003701	80.01	gold quality
blood	UBERON:0000178	79.30	gold quality
fundus of stomach	UBERON:0001160	75.91	gold quality
endometrium	UBERON:0001295	75.12	gold quality
myometrium	UBERON:0001296	74.48	gold quality
gastrocnemius	UBERON:0001388	73.94	gold quality
stomach	UBERON:0000945	72.75	gold quality
monocyte	CL:0000576	72.73	gold quality
adrenal gland	UBERON:0002369	72.51	gold quality
heart left ventricle	UBERON:0002084	72.38	gold quality
heart	UBERON:0000948	72.04	gold quality
liver	UBERON:0002107	71.87	gold quality
body of uterus	UBERON:0009853	71.66	gold quality
ovary	UBERON:0000992	71.44	gold quality
left ovary	UBERON:0002119	71.30	gold quality
right adrenal gland cortex	UBERON:0035827	71.09	gold quality
islet of Langerhans	UBERON:0000006	70.95	gold quality
left adrenal gland	UBERON:0001234	70.84	gold quality
urinary bladder	UBERON:0001255	70.75	gold quality
body of pancreas	UBERON:0001150	70.64	gold quality
fallopian tube	UBERON:0003889	70.62	gold quality
body of stomach	UBERON:0001161	70.28	gold quality
right atrium auricular region	UBERON:0006631	70.28	gold quality
left uterine tube	UBERON:0001303	70.03	gold quality
adult mammalian kidney	UBERON:0000082	69.93	gold quality
right adrenal gland	UBERON:0001233	69.90	gold quality
left adrenal gland cortex	UBERON:0035825	69.78	gold quality
lung	UBERON:0002048	69.68	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.47

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• Results suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation. (PMID:21687927)
• miR-32 blockade is sufficient to elevate Bim expression and sensitize acute myeloid leukemia cells to chemotherapy-induced apoptosis (PMID:21816906)
• MIR32 is upregulated in prostate cancer and regulates expression of BTG2. (PMID:22266859)
• results define miR-25 and -32 as positive regulators of p53, underscoring their role in tumorigenesis in glioblastoma (PMID:22431589)
• A novel mechanism of HIV-1 Tat C protein-mediated perturbation of microRNA-32 results in dysregulation of cellular TRAF3. (PMID:22709905)
• MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells. (PMID:23617834)
• MiR-32 overexpression is correlated with colorectal cancer (CRC) clinicopathological features and is a marker of poor prognosis. MiR-32 and PTEN expression are inversely correlated, miR-32 is associated with the development of CRC. (PMID:24123284)
• EZH2 was identified as the functional downstream target of miR-32. (PMID:25472588)
• Down-expression of PTEN could attenuate the inhibitory effects of knockdown miR-32 on the proliferation, migration, and invasion of liver cancer cells, suggesting that miR-32 could be a potential target for hepatocellular carcinoma treatment (PMID:25647261)
• Our results provided the first evidence that down-regulation of miR-32 was correlated with human non-small cell lung cancer progression (PMID:25755781)
• Suggest that direct PHLPP2 downregulation is required for miR-32-induced cell proliferation of breast cancer cells. (PMID:26276160)
• conclude that miR-32 promotes GC cell proliferation, migration and invasion by targeting KLF4, suggesting that the miR-32-KLF4 pathway may be useful in clinical diagnosis and therapeutics (PMID:26471298)
• Three circulating up-regulated microRNAs, miR-197-3p, miR-1281 and miR-32-3p, are proposed as potential new malignant pleural mesothelioma biomarkers. (PMID:27716620)
• A negative correlation between miR-32 and KLF4 expression was detected in gastric cancer cells. (PMID:27871067)
• MiR-32-5p facilitated intracellular mycobacterial survival and attenuated cytokine production in M.tb-infected macrophages. MiR-32-5p directly targets FSTL1. (PMID:28215633)
• Results confirmed miR-32 as a critical modulator of vascular calcification through its targeting of the PTEN/Akt/RUNX2 axis in mice. Moreover, results of analyses of plasma from CAD patients revealed that plasma miR-32 levels were positively correlated with coronary artery calcification (CAC) and might serve as a diagnostic marker in coronary artery disease patients with CAC. (PMID:28319142)
• Study highlights the importance of hsa-miR-32-5p, which is up-regulated in cancerous oral squamous cell carcinoma (OSCC) tissue and was previously reported to be up-regulated in serum of OSCC patients. (PMID:29330429)
• The present study suggested that miR32 may effectively inhibit WWP2 expression in human amniotic epithelial stem cells and promote Oct4 overexpression to maintain their pluripotency. (PMID:29393344)
• Suggest a significant role of miR-32/BMP5 axis in colorectal cancer tumorigenesis. (PMID:30119170)
• KLF4 is an essential regulator in cisplatin-induced apoptosis, and the miR-32-5p-KLF4-BIK signalling axis plays an important role in prostate cancer chemosensitivity (PMID:30176890)
• Data discovered an increased expression of miR-32 in esophageal squamous cell carcinoma (ESCC) tumors and cell lines. miR-32 could promote ESCC migration, invasion, and adhesion via suppressed CXXC5, and overexpression of CXXC5 could stimulate TGF-beta signaling, suggesting that miR-32 might be a key oncogene of ECSS metastasis through inhibiting CXXC5-mediated TGF-beta pathway. (PMID:30362164)
• Data indicate that miR-32 accelerates progression in HCC by targeting ADAMTS9, and the abnormal expression of miR-32 is correlated with prognosis. (PMID:30393368)
• miR-32-5p significantly downregulated in cervical cancer (CCa) tissues and cells. Bioinformatics and Luciferase method screened HOXB8 as a downstream regulatory target of miR-32-5p. Besides, HOXB8 was incredibly highly expressed in CCa tissues and cells. Decreased expression of HOXB8 resulting from upregulation of miR-32-5p could weaken cell proliferation, clone formation, invasion and migration ability of HeLa cells. (PMID:30657550)
• miR-32 acts an important role in inhibiting glioma cell proliferation and metastasis and suppresses the expression of ABCC4 by directly targeting its 3’-untranslated region. The miR-32/enhancer of zeste homolog 2 axis may provide new insights to the treatment for glioma. (PMID:31138033)
• Identification and development of long non-coding RNA-associated regulatory network in colorectal cancer. (PMID:31144439)
• miR-32 negatively modulates PTEN, thereby promoting M2 macrophage transformation through PI3K/AKT signaling, enhancing glioma proliferation and migration abilities. (PMID:31218569)
• MicroRNA 32 promotes cell proliferation, migration, and suppresses apoptosis in colon cancer cells by targeting OTU domain containing 3. (PMID:31338872)
• The authors show positive effects of miR-32-5p on cell proliferation and the phenotypic changes in cardiac fibroblasts by inhibiting DUSP1 (Dual-specificity protein phosphatase 1) expression. (PMID:31438862)
• Mir-32 role in the tumorigenesis and metastasis of colorectal cancer.SNHG14 promotes the tumorigenesis and metastasis of colorectal cancer through miR-32-5p/ski-oncogene-like SKIL axis.SKIL is a downstream target gene of miR-32-5p. (PMID:31471872)
• Elevation of plasma miR-32 levels in Non-Small Cell Lung Cancer patients receiving platinum-based chemotherapy can predict the sensitivity of chemotherapy regimens and predict the prognosis of patients. Thus, changes of plasma miR-32 levels in platinum-based chemotherapy patients with lung cancer are a prognostic indicator. (PMID:31626089)
• miR-32-5p has a role in H. pylori-related pediatric enteritis, which involves TAK1 and p38 (PMID:31749593)
• Long non-coding RNA SNHG5 regulates chemotherapy resistance through the miR-32/DNAJB9 axis in acute myeloid leukemia. (PMID:31884339)
• Silence of miR-32-5p promotes endothelial cell viability by targeting KLF2 and serves as a diagnostic biomarker of acute myocardial infarction. (PMID:32127011)
• Study of the Association Between microRNA (miR-25T>C, miR-32C>A, miR-125C>T, and miR-222G>T) Polymorphisms and the Risk of Recurrent Pregnancy Loss in Korean Women. (PMID:32224893)
• LncRNA WEE2-AS1 promotes proliferation and inhibits apoptosis in triple negative breast cancer cells via regulating miR-32-5p/TOB1 axis. (PMID:32307083)
• Overexpression of miR-32 inhibits the proliferation and metastasis of ovarian cancer cells by targeting BTLA. (PMID:32432730)
• Long noncoding RNA PITPNAAS1 silencing suppresses proliferation, metastasis and epithelialmesenchymal transition in nonsmall cell lung cancer cells by targeting microRNA325p. (PMID:33495838)
• CUL4B renders breast cancer cells tamoxifen-resistant via miR-32-5p/ER-alpha36 axis. (PMID:33638154)
• MiR-32-3p Regulates Myocardial Injury Induced by Microembolism and Microvascular Obstruction by Targeting RNF13 to Regulate the Stability of Atherosclerotic Plaques. (PMID:34185281)
• Aberrant expression profile of miR-32, miR-98 and miR-374 in chronic lymphocytic leukemia. (PMID:34455196)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir32	ENSMUSG00000065544

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.