MIR320A

Summary

MIR320A (microRNA 320a, HGNC:31632) is a microRNA gene on chromosome 8p21.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 407037 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31632
Approved symbol	MIR320A
Name	microRNA 320a
Location	8p21.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-320
Ensembl gene	ENSG00000208037
Ensembl biotype	miRNA
OMIM	614112
Entrez	407037
RNAcentral	URS0000D53E88 — miRNA, 72 nt, 2 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385302

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385302 — 1 exons

Exon	Start	End
ENSE00001500308	22244962	22245043

Expression profiles

Bgee: expression breadth broad, 95 present calls, max score 89.47.

Top tissues by expression

95 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	89.47	gold quality
adrenal tissue	UBERON:0018303	82.19	gold quality
bone marrow	UBERON:0002371	79.63	gold quality
granulocyte	CL:0000094	78.73	gold quality
smooth muscle tissue	UBERON:0001135	76.81	gold quality
placenta	UBERON:0001987	75.53	gold quality
liver	UBERON:0002107	75.53	gold quality
monocyte	CL:0000576	75.48	gold quality
muscle of leg	UBERON:0001383	73.97	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	73.69	gold quality
gastrocnemius	UBERON:0001388	73.65	gold quality
right lobe of liver	UBERON:0001114	72.92	gold quality
blood	UBERON:0000178	72.18	gold quality
islet of Langerhans	UBERON:0000006	71.83	gold quality
left coronary artery	UBERON:0001626	71.81	gold quality
endometrium	UBERON:0001295	71.35	gold quality
tonsil	UBERON:0002372	71.29	gold quality
left uterine tube	UBERON:0001303	71.28	gold quality
descending thoracic aorta	UBERON:0002345	71.11	gold quality
heart left ventricle	UBERON:0002084	70.45	gold quality
heart	UBERON:0000948	69.85	gold quality
body of pancreas	UBERON:0001150	69.43	gold quality
right uterine tube	UBERON:0001302	69.07	gold quality
transverse colon	UBERON:0001157	69.06	gold quality
right adrenal gland cortex	UBERON:0035827	68.98	gold quality
right atrium auricular region	UBERON:0006631	68.95	gold quality
tibial artery	UBERON:0007610	68.91	gold quality
urinary bladder	UBERON:0001255	68.56	gold quality
amygdala	UBERON:0001876	68.39	gold quality
fallopian tube	UBERON:0003889	68.36	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.72

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• TEL-AML1 might exert its antiapoptotic action at least in part by suppressing miRNA-494 and miRNA-320a, lowering their expression causing enhanced survivin expression. (PMID:20807887)
• These findings indicate that miR-320a suppresses the growth of colon cancer cells by directly targeting beta-catenin, suggesting its application in prognosis prediction and cancer treatment. (PMID:22459450)
• These findings suggest that oxidative stress-responsive microRNA-320a may regulate glycolysis broadly within nature. (PMID:22767230)
• miR-320 is a key negative regulator in prostate tumor initiating cells. (PMID:23188675)
• MiR-320a is downregulated in patients with myasthenia gravis and modulates inflammatory cytokines production by targeting mitogen-activated protein kinase 1. (PMID:23196978)
• During hemin-induced erythroid differentiation, enhanced expression of SMAR1 negatively correlates with miR-320a expression. (PMID:23876508)
• these data show that miR-320 regulates the function of vascular endothelial cells by targeting NRP1 and has the potential to be developed as an anti-angiogenic or anti-cancer drug. (PMID:24114198)
• study found that low expression of miR-320a was correlated with short time to imatinib resistance in patients with gastrointestinal stromal tumors (PMID:24217767)
• results demonstrate that miR-320a functions as a tumour-suppressive miRNA through targeting Rac1 in colorectal cancer (PMID:24265291)
• Study demonstrates that pre-miR-320 is an RNA Pol II transcript that is 7-methylguanosine (m7G)-capped at its 5’ end, whereas its 3’ end is most likely produced by transcription termination. (PMID:24360278)
• High MIRN320 expression is associated with Glioblastoma multiforme. (PMID:24435880)
• MiR-320a down-regulation mediates bladder carcinoma invasion by targeting ITGB3. (PMID:24443232)
• E2A is an independent prognostic factor for colorectal cancer patients and targets miR-320a to regulate cell proliferation of colon cancer cells. (PMID:24454819)
• miR-320a has a role in modulating the induction of HO-1, GCLM and OKL38 by oxidized phospholipids in endothelial cells (PMID:24786516)
• In colorectal adenomatous polyps, the expression of miR-320a increased and miR-145 and miR-192 expression decreased with higher histologic grade. (PMID:24791633)
• MiR-320 regulates the expression and functions of E2F1 and SF-1 in Polycystic Ovary Syndrome patients. (PMID:24828505)
• Low miR-320a expression was associated with glioma. (PMID:25117070)
• miR-320a directly and functionally targets BMI-1 in nasopharyngeal carcinoma. Overexpression of miR-320a reduces migration and invasion of NPC cells. (PMID:25171860)
• miR-320a specifically targeted the 3’ UTR of Hsc 70, decreased Hsc 70 expression and induced alpha-synuclein intracellular accumulation. (PMID:25207598)
• the mi-RNAs hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p were induced in human umbilical vein endothelial cells following expression of EBOV GP (PMID:25218824)
• Luciferase assay validated RUNX2 (Runt-related transcription factor 2) as a bona fide target for miR-320 family. (PMID:25356868)
• Waldenstrom macroglobulinemia can be differentially diagnosed using a combination of serum microRNA-320a and microRNA-320b expression levels. (PMID:25428891)
• These findings implied that miR-320-FOXM1 axis may overcome chemo-resistance of colon cancer cells. (PMID:25446103)
• Low MicroRNA-320a expression is associated with metastasis in colorectal cancer. (PMID:25458952)
• This study demonistrated that expression of miR-320a is decreased in B cells of MS patients and may contribute to increased blood-brain barrier permeability and neurological disability. (PMID:25468268)
• Data show that microRNA miR-320a is a key regulator of rtherogenesis, and down-regulating serum response factor (SRF). (PMID:25728840)
• Data show that micrRNA miR-320a directly targets cAMP-regulated phosphoprotein 19 (ARPP-19) and estrogen-related receptor gamma protein (ERRgamma) in breast cancer cell lines. (PMID:25736597)
• miR320 expression is downregulated in patients with glioma. (PMID:25901521)
• MiR-320a inhibits metastasis in salivary Adenoid cystic carcinoma by targeting ITGB3 and may serve as a therapeutic target and prognostic marker in salivary cancers. (PMID:25924850)
• Study found down-regulation of miR-320a and up-regulation of miR-451a in drug naive, first episode depressed patients (PMID:26343596)
• Mir-320a is associated with diabetic retinopathy incidence and progression in type 1 diabetes patients. (PMID:26395742)
• serum starvation induces CREB1 expression, in turn activating miR-320a expression, which then down-regulates VDAC1 expression to facilitate mitophagy (PMID:26472185)
• The accumulation of NLC1-C in the nucleus repressed miR-320a and miR-383 transcript and promoted testicular embryonal carcinoma cell proliferation by binding to Nucleolin. (PMID:26539909)
• Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC, whereas miR-320a inhibition had the opposite effects. (PMID:26741129)
• Decreased Expression of miR320 is associated with Inflammatory Bowel Disease. (PMID:26752466)
• MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1beta-induced chondrocyte responses. (PMID:26774733)
• Our findings collectively indicate that miR-320a inhibits cell proliferation and induces apoptosis in MM cells by directly targeting PBX3, supporting its utility as a novel and potential therapeutic agent for miRNA-based MM therapy. (PMID:27086852)
• These results thus reveal the crucial role played by miR-320a in limiting gastric carcinoma by directly targeting FoxM1- P27KIP1 axis (PMID:27086911)
• Data indicate a role for the miR-320/SOX4/FOXM1/FOXQ1 axes in promoting colorectal cancer (CRC) development and suggest targeting those networks as potential therapeutic strategy for CRC. (PMID:27119506)
• Overexpression of LAMP1 was observed in prostate cancer (PCa) and castration-resistant prostate cancer clinical specimens. Moreover, downstream pathways were identified using si-LAMP1-transfected cells. The discovery of tumor-suppressive miR320a-mediated pathways may provide important insights into the potential mechanisms of PCa metastasis. (PMID:27212625)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
rattus_norvegicus	Mir320a	ENSRNOG00000035629

Paralogs (5): MIR320E (ENSG00000211513), MIR320B1 (ENSG00000211543), MIR320C2 (ENSG00000212051), MIR320D2 (ENSG00000221081), MIR320B2 (ENSG00000221406)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.