MIR323A

Summary

MIR323A (microRNA 323a, HGNC:31766) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442897 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31766
Approved symbol	MIR323A
Name	microRNA 323a
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-323
Ensembl gene	ENSG00000199069
Ensembl biotype	miRNA
Entrez	442897
RNAcentral	URS000075AB81 — miRNA, 86 nt, 45 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362199

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362199 — 1 exons

Exon	Start	End
ENSE00001436962	101025732	101025817

Expression profiles

Bgee: expression breadth broad, 37 present calls, max score 93.17.

Top tissues by expression

37 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
colon	UBERON:0001155	93.17	gold quality
adrenal tissue	UBERON:0018303	91.16	gold quality
intestine	UBERON:0000160	85.68	gold quality
stomach	UBERON:0000945	79.72	gold quality
heart	UBERON:0000948	72.77	gold quality
islet of Langerhans	UBERON:0000006	72.63	gold quality
lung	UBERON:0002048	72.20	gold quality
blood	UBERON:0000178	70.70	gold quality
body of pancreas	UBERON:0001150	69.68	gold quality
ascending aorta	UBERON:0001496	68.80	gold quality
body of stomach	UBERON:0001161	68.43	gold quality
left adrenal gland	UBERON:0001234	67.38	gold quality
thoracic aorta	UBERON:0001515	67.14	gold quality
liver	UBERON:0002107	66.97	gold quality
skin of abdomen	UBERON:0001416	66.70	gold quality
putamen	UBERON:0001874	66.65	gold quality
omental fat pad	UBERON:0010414	65.90	gold quality
tibial artery	UBERON:0007610	65.68	gold quality
right hemisphere of cerebellum	UBERON:0014890	65.50	gold quality
nucleus accumbens	UBERON:0001882	65.39	gold quality
left ovary	UBERON:0002119	64.78	gold quality
anterior cingulate cortex	UBERON:0009835	64.77	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	64.41	gold quality
Ammon’s horn	UBERON:0001954	64.40	gold quality
cerebellar hemisphere	UBERON:0002245	64.36	gold quality
subcutaneous adipose tissue	UBERON:0002190	63.97	gold quality
skin of leg	UBERON:0001511	63.41	gold quality
urinary bladder	UBERON:0001255	63.05	gold quality
hypothalamus	UBERON:0001898	62.75	gold quality
caudate nucleus	UBERON:0001873	61.70	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 27)

• the authors identified miR-221/222 and miR-323-3p as novel dysregulated miR in rheumatoid arthritis synovial fibroblasts (PMID:22562984)
• results demonstrates that miR-326, miR-130a, miR-155, miR-210 and the 4 miRNA-interactions could serve as prognostic and predictive markers for survival of GBM patients (PMID:23302469)
• Circulating miR-323-3p was markedly elevated in acute coronary syndrome patients for at least one year. (PMID:25124998)
• Over-expression of miR-323-5p could promote apoptosis of U251 cells and inhibit the proliferation and migration of the glioma cells. miR-323-5p directly targets IGF1R. (PMID:25556445)
• miR-323 may increase VEGF-A-mediated cancer vascularization in PC cells through AdipoR1 suppression. (PMID:26160610)
• MiRNA-323-5p inhibited human cerebral glioma U373 cell proliferation and promoted its apoptosis by reducing IGF-1R (PMID:26656446)
• Low miR323 expression is associated with invasion and metastasis in pancreatic ductal adenocarcinoma. (PMID:26908446)
• Our data suggest that miR-323-3p acts in a negative feedback loop to control the production of IL-22 in IL-22/IL-17-producing T cells and might thus impact the T-cell responses in asthma. (PMID:27059796)
• This study demonstrated that overexpression of miR-323a-3p and miR-369-3p in glioblastoma cells inhibited their proliferation and migration in vitro. Mice implanted with glioblastoma cells overexpressing miR323a-3p. (PMID:27573219)
• The expression of miR-323a-5p was significantly elevated in the cortex and plasma of FCD patients. These results suggest that abnormal expression of miR-323a-5p could be used for improving the current diagnosis of FCD. (PMID:27824513)
• High miR323 expression is associated with cardiomyopathy in Friedreich’s ataxia. (PMID:28701783)
• Study demonstrates a novel regulatory mechanism of the miR-323a-3p/MET/SMAD3/SNAIL circuit that is involved in the EMT regulation of BCa. (PMID:28837140)
• T-C haplotype, increasing miR-323b expression, could down-regulated Pax8 expression. (PMID:29271476)
• miR-323a-3p suppresses growth of osteosarcoma cells via targeting lactate dehydrogenase A and inhibits the glycolysis of osteosarcoma. (PMID:30088225)
• Results show that the level of miR-323 in human cumulus cells (CCs) of women with polycystic ovary syndrome (PCOS) was down-regulated, compared with that of the control group. Further findings suggest that miR-323 targeting IGF-1 regulates the steroidogenesis and the activity of CCs, which plays an important role in the occurrence and development of PCOS. (PMID:30300681)
• miR323 suppresses nerve cell apoptosis in cerebral infarction via the TGFbeta1/SMAD3 signaling pathway. (PMID:30535466)
• miR-323a-5p and miR-342-5p are new tumor-suppressive microRNAs for neuroblastoma. (PMID:30770954)
• miR-323a expression in bladder cancer (BC) contributed to enhanced BC cell proliferation and migration mainly by targeting c-Met. (PMID:31180621)
• miR-323-3p promotes proliferation and inhibits apoptosis of cumulus cells in polycystic ovary syndrome (PMID:31549864)
• MiR-323-3p Targeting Transmembrane Protein with EGF-Like and 2 Follistatin Domain (TMEFF2) Inhibits Human Lung Cancer A549 Cell Apoptosis by Regulation of AKT and ERK Signaling Pathways. (PMID:32009129)
• Long non-coding RNA SNHG7 promotes neuroblastoma progression through sponging miR-323a-5p and miR-342-5p. (PMID:32534305)
• miR-323a regulates ERBB4 and is involved in depression. (PMID:33219358)
• Suppression of microRNA-323-3p restrains vascular endothelial cell apoptosis via promoting sirtuin-1 expression in coronary heart disease. (PMID:33460661)
• Identification of serum exosomal miR-98-5p, miR-183-5p, miR-323-3p and miR-19b-3p as potential biomarkers for glioblastoma patients and investigation of their mechanisms. (PMID:34837760)
• MiR-323a regulates ErbB3/EGFR and blocks gefitinib resistance acquisition in colorectal cancer. (PMID:35319011)
• miRNA-323a-3p promoted intracranial, aneurysm-induced inflammation via AMPK/NF-kappaB signaling pathway by AdipoR1. (PMID:36047894)
• Tumor-suppressive miR-323a inhibits pancreatic cancer cell proliferation and glycolysis through targeting HK-2. (PMID:36416387)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir323	ENSMUSG00000065617
rattus_norvegicus	Mir323	ENSRNOG00000035454

Paralogs (16): MIR494 (ENSG00000194717), MIR377 (ENSG00000199015), MIR381 (ENSG00000199020), MIR369 (ENSG00000199025), MIR410 (ENSG00000199092), MIR409 (ENSG00000199107), MIR539 (ENSG00000202560), MIR487A (ENSG00000207742), MIR487B (ENSG00000207754), MIR656 (ENSG00000207959), MIR496 (ENSG00000207961), MIR154 (ENSG00000207978), MIR323B (ENSG00000208004), MIR1185-1 (ENSG00000221525), MIR1185-2 (ENSG00000221614), MIR382 (ENSG00000283170)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.