MIR325

Summary

MIR325 (microRNA 325, HGNC:31768) is a microRNA gene on chromosome Xq21.1.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442899 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31768
Approved symbol	MIR325
Name	microRNA 325
Location	Xq21.1
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-325
Ensembl gene	ENSG00000207995
Ensembl biotype	miRNA
Entrez	442899
RNAcentral	URS000075BDCE — miRNA, 98 nt, 10 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385260

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385260 — 1 exons

Exon	Start	End
ENSE00001500266	77005404	77005501

Expression profiles

Bgee: expression breadth broad, 40 present calls, max score 83.61.

Top tissues by expression

40 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.61	gold quality
heart	UBERON:0000948	74.21	gold quality
intestine	UBERON:0000160	72.26	gold quality
lower esophagus muscularis layer	UBERON:0035833	71.99	gold quality
body of pancreas	UBERON:0001150	71.68	gold quality
calcaneal tendon	UBERON:0003701	71.07	gold quality
subcutaneous adipose tissue	UBERON:0002190	69.79	gold quality
stomach	UBERON:0000945	69.30	gold quality
right atrium auricular region	UBERON:0006631	69.16	gold quality
lung	UBERON:0002048	69.12	gold quality
Brodmann (1909) area 9	UBERON:0013540	68.62	gold quality
thoracic aorta	UBERON:0001515	68.25	gold quality
ascending aorta	UBERON:0001496	67.98	gold quality
Ammon’s horn	UBERON:0001954	67.45	gold quality
putamen	UBERON:0001874	67.15	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	67.14	gold quality
right adrenal gland	UBERON:0001233	66.64	gold quality
adenohypophysis	UBERON:0002196	66.32	gold quality
body of uterus	UBERON:0009853	66.31	gold quality
corpus callosum	UBERON:0002336	66.25	gold quality
left ovary	UBERON:0002119	66.03	gold quality
transverse colon	UBERON:0001157	65.90	gold quality
substantia nigra	UBERON:0002038	65.88	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	65.75	gold quality
prefrontal cortex	UBERON:0000451	65.42	gold quality
thoracic mammary gland	UBERON:0005200	65.26	gold quality
brain	UBERON:0000955	65.04	gold quality
hypothalamus	UBERON:0001898	63.68	gold quality
amygdala	UBERON:0001876	63.63	gold quality
omental fat pad	UBERON:0010414	63.61	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.49

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 13)

• these results suggest that the genetic polymorphism pri-miR-325 is associated with functional dyspepsia (FD) and interacts with SLC6A4 polymorphisms in increasing susceptibility to FD in Japanese. (PMID:22438098)
• The downregulated hsa-miR-325 significantly correlated with diastolic blood pressure and body mass index. (PMID:22710575)
• Single nucleotide polymorphism of TRPV1 315G>C, rs5981521 of pri-miR-325 and SCN10A is related to the development of functional dyspepsia. This involvement differed between Helicobater pylori-positive and -negative patients. (PMID:23047628)
• The most pronounced effects were observed for hsa-miR-146a-5p, which was upregulated by four of the 16 investigated fatty acids, and hsa-miR-32-5p, which was strongly downregulated by five fatty acids in the human colorectal adenocarcinoma cell line Caco-2. (PMID:25612549)
• These findings implied that miR-325-3p regulates cell invasion and proliferation via targeting HMGB1 and may be a potential prognostic marker for non-small cell lung cancer. (PMID:25776482)
• Human microRNA-325 is lowly expressed and may serve as a potential prognostic biomarker in human bladder cancer. (PMID:30176759)
• KIF2C exerts an oncogenic role in nonsmall cell lung cancer and is negatively regulated by miR-325-3p (PMID:31328811)
• miR-325-3p Overexpression Inhibits Proliferation and Metastasis of Bladder Cancer Cells by Regulating MT3. (PMID:32512576)
• Suppression of HAX-1 induced by miR-325 resensitizes bladder cancer cells to cisplatin-induced apoptosis. (PMID:33015771)
• MiR-325-3p mediate the CXCL17/CXCR8 axis to regulate angiogenesis in hepatocellular carcinoma. (PMID:33515898)
• LINC01515 promotes nasopharyngeal carcinoma progression by serving as a sponge for miR-325 to up-regulate CDCA5. (PMID:33770322)
• MiR-325-3p Alleviates Acute Pancreatitis via Targeting RIPK3. (PMID:35094251)
• miR-325 Supresses Cell Proliferation and Migration in Non-Small Cell Lung Cancer via Targeting DNA Ligase 1 (LIG1). (PMID:39231317)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.