MIR335

Summary

MIR335 (microRNA 335, HGNC:31773) is a microRNA gene on chromosome 7q32.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The encoded miRNA is dysregulated in a variety of cancers, including breast, colorectal, and prostate cancer. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 442904 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31773
Approved symbol	MIR335
Name	microRNA 335
Location	7q32.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-335
Ensembl gene	ENSG00000199043
Ensembl biotype	miRNA
OMIM	611768
Entrez	442904
RNAcentral	URS000063D0D0 — miRNA, 94 nt, 3 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362173

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362173 — 1 exons

Exon	Start	End
ENSE00001436936	130496111	130496204

Expression profiles

Bgee: expression breadth tissue_specific, 3 present calls, max score 54.61.

Top tissues by expression

3 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal glomerulus	UBERON:0000074	54.61	silver quality
pituitary gland	UBERON:0000007	54.30	silver quality
placenta	UBERON:0001987	51.14	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ID4, SP1

Literature-anchored findings (GeneRIF, showing 40)

• our data suggest that the two genetic variants within miR-126 and miR-335 are not associated with breast cancer risk. (PMID:21046227)
• miR-335 downregulation is critical for the acquisition of reparative MSC phenotypes. (PMID:21164520)
• implicate the miR-335 locus on 7q32.2 as the first selective metastasis suppressor and tumor initiation suppressor locus in human breast cancer (PMID:21289068)
• miR-139-3p, pmiR-675, and miR-335 allowed separation of adrenocortical adenomas from adrenocortical carcinomas (PMID:21471143)
• our data indicate that miR-335 affects different targets in the upstream BRCA1-regulatory cascade with impact on key cellular functions such as proliferation and apoptosis. (PMID:21618216)
• our results provide evidence that miR-335 might function as a metastasis suppressor in gastric cancer by targeting SP1 directly and indirectly through the Bcl-w-induced phosphoinositide 3-kinase-Akt-Sp1 pathway (PMID:21822301)
• findings suggest that miR-335 is potently required for differentiation of malignant glioma cells induced by cAMP/PKA pathway activation, and a single microRNA may act as an important fate determinant to control differentiation status of malignant gliomas (PMID:22172575)
• The MYCN transcription factor directly binds to a region immediately upstream of the miR-335 transcriptional start site. (PMID:22382496)
• These results indicated that miR-335 expression was increased in human gliomas and was associated with advanced tumor progression (PMID:22644918)
• A high frequency recurrence and poor survival were observed in gastric cancer cases with high level of hsa-miR-335 (PMID:22802949)
• High miR-335 expression is associtated with meningiomas. (PMID:22886530)
• The expression levels of miR-335 significantly correlated with those of MEST, supporting the notion that the intronic miR-335 is co-expressed with its host gene (PMID:23229728)
• this study validated the functions of the rs2239680 SNP in the mir-355-related regulation of BIRC5 in Chinese Han with lung cancer. (PMID:23232114)
• we disclose a miR-129-2(miR-335)/SOX4/Semaphorin-Plexin regulatory axis in the tumorigenesis of pancreatic cancer. (PMID:23251334)
• Investigated functional and clinical significance of miR-335 in PCa. Reduced expression of miR-335 was found in human PCa compared with benign prostate tissues.Inc’d miR-335 exp’n suppressed cell proliferation, invasion & migration of PCa cell lines. (PMID:23456549)
• Taken together, these findings strongly suggest that an ATM-dependent CREB-miR-335-CtIP axis influences the selection of HRR for repair of certain DSB lesions (PMID:23696749)
• MiR-335 lacks of expression brings about the abnormal accumulation of Bcl-w. (PMID:23708561)
• Upregulation of miR-335 can simultaneously suppress the invasiveness and promote apoptosis of lung cancer cell A549 and H1299 by targeting Bcl-w and SP1. (PMID:23740614)
• The transcriptional regulation of miR-335 was identified potential promoter of miR-335 within the intron of mesoderm-specific transcript homolog. (PMID:23801157)
• GRP-R-mediated tumorigenicity and increased metastatic potential in neuroblastoma are regulated, in part, by miR-335 and miR-363. (PMID:23806264)
• Deregulated MIR335 that targets MAPK1 is implicated in poor outcome of paediatric acute lymphoblastic leukaemia. (PMID:23888996)
• loss of miR-335 promoted Small cell lung cancer metastatic skeletal lesions via deregulation of IGF-IR and RANKL pathways and was associated with metastatic osteolytic skeletal lesions. (PMID:23966614)
• miR-335 acts as tumor suppressor by targeting the ROCK1 gene and inhibiting osteosarcoma cells migration and invasion. (PMID:23975506)
• Data indicate that expression of miR-1260, -26a, -335*, -576-3p, -628-3p and -664 were consistently dysregulated in both whole blood and H1N1 infected cells. (PMID:24116168)
• miR-335 regulates the expression of at least five formin family members, three of which are validated, FMNL3, FMN2 and DAAM2. (PMID:24223803)
• investigation of clinical significance of miR-335 expression in epithelial ovarian cancer(EOC); miR-335 expression was reduced in malignant tissue samples, especially in omental metastases; low miR-335 levels in EOC were associated with shorter overall survival and relapse-free survival (PMID:24515774)
• Differences in the expression levels of miR-335 and MEST (median [interquartile range]: 1.69 [0.85-1.74], and 3.85 [3.20-5.67] were detected between mesenchymal stem cells isolated from patients with osteoarthritis and controls (PMID:24582835)
• Our results demonstrate that miR-335 plays a key role in the regulation of reparative activities of human mesenchymal stem cells (PMID:24648336)
• Molecular data on gastric cance patients show that down regulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p is correlated with extensive lymph node metastasis, lymphatic abd venous invasion, high-stage Borrmann type and poor differentiation. (PMID:24805774)
• MicroRNA-335 inhibits invasion and metastasis of colorectal cancer by targeting ZEB2.miR-335 was downregulated in human primary colorectal cancer tissues with lymph node metastases. (PMID:24829139)
• miR-335 might inhibit progression and stem cell properties of pancreatic cancer targeting OCT4 (PMID:24859837)
• Results show that microRNAs Let-7a and miR-335 expression levels were significantly downregulated in the tumors of patients with a BRCA mutation and correlated with tumor prognosis. (PMID:24942235)
• miR-335 is down regulated in a majority of esophageal squamous cell carcinoma patients. (PMID:25337272)
• Taken together, these results demonstrate that miR-335 suppresses breast cancer cell migration by negatively regulating the HGF/c-Met pathway. (PMID:25492484)
• The results suggested that miR-335 expression was decreased in cervical cancer specimens and lower miR-335 expression resulted in poorer survival in patients with cervical cancer. (PMID:25712373)
• these results indicate that miR-335 acts as a novel tumor suppressor to regulate ccRCC cell proliferation and invasion through downregulation of BCL-W expression. (PMID:25846734)
• miR-335 exerted apoptotic effects and inhibited ubiquitination of HDAC3 in anti-cancer drug-resistant cancer cell lines. miR-335 negatively regulated the invasion, migration, and growth rate of cancer cells. (PMID:25997740)
• Upregulation of microRNA335 and microRNA584 contributes to the pathogenesis of severe preeclampsia through downregulation of endothelial nitric oxide synthase (PMID:26133786)
• Data indicate that microRNA miR-335 can regulate cell surface membrane-type 1 matrix metalloproteinase (MT1-MMP) levels in some tumor cells, a property accompanied by increased motility and proliferation in these cells. (PMID:26204513)
• results suggest that miR-34a and miR-335 are candidate tumor suppressive miRNAs in GISTs, and that they are frequent targets of epigenetic silencing in GISTs. (PMID:26214687)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir335	ENSMUSG00000065501
rattus_norvegicus	rno-mir-335	ENSRNOG00000068719

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.