MIR33B

Summary

MIR33B (microRNA 33b, HGNC:32791) is a microRNA gene on chromosome 17p11.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 693120 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32791
Approved symbol	MIR33B
Name	microRNA 33b
Location	17p11.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-33b
Ensembl gene	ENSG00000207839
Ensembl biotype	miRNA
OMIM	613486
Entrez	693120
RNAcentral	URS000075D54F — miRNA, 96 nt, 8 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385104

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385104 — 1 exons

Exon	Start	End
ENSE00001500110	17813836	17813931

Expression profiles

Bgee: expression breadth broad, 79 present calls, max score 97.13.

Top tissues by expression

79 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	97.13	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	88.18	gold quality
skeletal muscle tissue	UBERON:0001134	83.03	gold quality
left uterine tube	UBERON:0001303	79.02	gold quality
left adrenal gland cortex	UBERON:0035825	78.90	gold quality
left adrenal gland	UBERON:0001234	78.70	gold quality
right adrenal gland	UBERON:0001233	78.50	gold quality
adrenal gland	UBERON:0002369	78.13	gold quality
right adrenal gland cortex	UBERON:0035827	77.09	gold quality
muscle of leg	UBERON:0001383	75.54	gold quality
monocyte	CL:0000576	75.40	gold quality
left coronary artery	UBERON:0001626	74.47	gold quality
lower esophagus mucosa	UBERON:0035834	74.42	gold quality
blood	UBERON:0000178	74.01	gold quality
gastrocnemius	UBERON:0001388	72.42	gold quality
heart	UBERON:0000948	71.92	gold quality
right atrium auricular region	UBERON:0006631	71.69	gold quality
body of stomach	UBERON:0001161	71.31	gold quality
liver	UBERON:0002107	71.22	gold quality
omental fat pad	UBERON:0010414	71.00	gold quality
esophagus mucosa	UBERON:0002469	70.71	gold quality
endometrium	UBERON:0001295	70.70	gold quality
adrenal tissue	UBERON:0018303	70.57	gold quality
heart left ventricle	UBERON:0002084	70.56	gold quality
right lobe of liver	UBERON:0001114	70.53	gold quality
body of pancreas	UBERON:0001150	70.29	gold quality
minor salivary gland	UBERON:0001830	69.96	gold quality
right ovary	UBERON:0002118	69.80	gold quality
esophagus	UBERON:0001043	69.74	gold quality
substantia nigra	UBERON:0002038	69.65	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.33

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• miR-33a/b embedded within introns of SREBP genes targets ABCA1, a regulator of HDL synthesis and reverse cholesterol transport, for posttranscriptional repression; indicates miR-33 acts in concert with SREBP host genes to control cholesterol homeostasis (PMID:20466882)
• miR-33a and -b regulate pathways controlling three of the risk factors of metabolic syndrome: levels of HDL, triglycerides, and insulin signaling (PMID:21576456)
• In this work, the authors identified miR-33b as a primate-specific negative regulator of c-Myc. (PMID:22887866)
• miR-33b regulates lipid metabolism through both arms of the miR-33a/miR-33b duplex. (PMID:23547260)
• miR-33b cooperates with SREBP1 in regulating glucose metabolism by targeting phosphoenolpyruvate carboxykinase (PCK1) and glucose-6-phosphatase (G6PC), key regulatory enzymes of hepatic gluconeogenesis. (PMID:23716591)
• Xuezhikang (XZK) therapy raises plasma levels of miR-33a and miR-33b, which may inhibit cellular cholesterol export and limit the HDL-raising effect of XZK. (PMID:24591767)
• We found that rs8066560 variant on SREBP-1 and miR-33b genes is associated with LDL-C levels; however, it may not be an important determinant for the MetS, hyperglycemia, and insulin resistance in children and adolescents. (PMID:24825092)
• Data show that microRNA-33b (miR-33b) is co-expressed with sterol regulatory element-binding protein 1 (SREBP1) in HepG2 cells. (PMID:24931346)
• MicroRNAs 758 and 33b are significantly upregulated in hypercholesterolemic plaques. (PMID:25445880)
• suppresses the epithelial-to-mesenchymal transition (EMT) and migratory potential of melanoma cells by targeting HMGA2 (PMID:25725129)
• Methyl protodioscin increases ABCA1 expression and cholesterol efflux while inhibiting gene expressions for synthesis of cholesterol and triglycerides by suppressing SREBP transcription and microRNA 33a/b levels. (PMID:25733328)
• MicroRNA-33a/b in lipid metabolism (PMID:25744742)
• targeting miRNA by cordycepin indicates a new mechanism of cordycepin-induced suppression of tumor metastasis; miR-33b/HMGA2/Twist1/ZEB1 axis plays critical roles in regulating melanoma dissemination (PMID:25868853)
• The mechanism through which miR-33b inhibits the stemness, migration and invasion of breast cancer cells is by targeting HMGA2, SALL4 and Twist1. (PMID:25919570)
• Expression of miR-33a/b increased in human carotid atherosclerotic plaques. There was a decrease in mitochondrial regulatory genes PGC-1alpha, SLC25A25, NRF1, and TFAM. In THP-1 cells, miR-33 directly regulates the expression of PGC-1alpha, SLC25A25, and PDK4. (PMID:26002865)
• miR-33b located in the intron of sterol-regulatory elementbinding protein (SREBF1), control cholesterol homeostasis. (PMID:26084456)
• Circulating levels of miR-33a and miR-33b are up-regulation in children with familial hypercholesterolemia. (PMID:26229086)
• the present results outline a possible role and mechanism of miR-33b in suppression of lung adenocarcinoma progression (PMID:26459797)
• miR-33b acts as a tumor suppressor and hypermethylation of the CpG island upstream of miR-33b is responsible for its down-regulation in gastric cancer. (PMID:26729612)
• miR-33b is highly induced upon differentiation of human preadipocytes, along with SREBP-1 and miR-33b is an important regulator of adipogenesis (PMID:26830228)
• High plasma MicroRNA33b in Type 2 Diabetic Patients is associated with Dyslipidemia. (PMID:27301461)
• High plasma MicroRNA33b in Type 2 Diabetic Patients is associated with Lipid Disorders. (PMID:27476669)
• CDKN1A (p21) was identified as a functional target of miR-33b-3p, a critical regulator of G1/S checkpoint, which potentially mediated the protection effects of miR-33b-3p against cisplatin. (PMID:27559850)
• The expression of miR-33b decreases and HMGA2 increases in the esophageal squamous cell carcinoma. (PMID:27609581)
• Hepatic miR33b expression was increased in morbidly obese compared to moderately obese and in nonalcoholic steatohepatitis. (PMID:27669236)
• miR-33 regulates cellular macrophage cholesterol homeostasis and autophagy in atherosclerosis. (PMID:28428217)
• Results reveal that miR-33b, miR-671, and miR-140 are significantly downregulated in the serum of osteoarthritis patients. (PMID:29255496)
• Results demonstrated that miR-33a/b might play an important role in cholesterol regulation. (PMID:29601916)
• The study reveals that miR-33b plays a suppressive role in the regulation of osteosarcoma cell proliferation through direct targeting LDHA. (PMID:30213286)
• SREBF1-miR-33b axis affects both lipid profiles and macrophage phenotype remodeling, promoting the development of atherosclerosis. (PMID:30354203)
• Downregulation of miR-33b promotes non-small cell lung cancer cell growth through reprogramming glucose metabolism by targeting LDHA. (PMID:30368888)
• Ageing influenced the relationship of circulating miR-33 a and miR-33 b with insulin resistance and increased adiposity. (PMID:30537863)
• Authors demonstrate that CUL4B upregulates the expression of C-MYC at post-transcriptional level through epigenetic silencing of miR-33b-5p. CUL4B-induced oncogenic activity in PCa by targeting C-MYC is repressed by miR-33b-5p. (PMID:30609075)
• miR33b upregulation inhibited cell growth and enhanced apoptosis in an Ems model (primary cell cultures) compared with the control group. In addition, miR33b upregulation reduced Wnt/betacatenin signaling pathway and suppressed zinc finger Eboxbinding homeobox 1 (ZEB1) protein expression in the in vitro Ems model (primary cell cultures) compared with the control group (PMID:30664209)
• Possible roles of three miRNAs (mir142, mir33b, mir423) in polycystic ovary syndrome (PCOS) and might provide candidate biomarkers for PCOS diagnosis. (PMID:30865275)
• miR-33-5p knockdown attenuates abdominal aortic aneurysm progression via promoting target adenosine triphosphate-binding cassette transporter A1 expression and activating the PI3K/Akt signaling pathway. (PMID:31170866)
• MicroRNA-33b regulates sensitivity to daunorubicin in acute myelocytic leukemia by regulating eukaryotic translation initiation factor 5A-2. (PMID:31222822)
• Identification of Differential Roles of MicroRNA-33a and -33b During Atherosclerosis Progression With Genetically Modified Mice. (PMID:31242815)
• MiR-33b-3p promotes chondrocyte proliferation and inhibits chondrocyte apoptosis and cartilage extracellular matrix degradation by targeting DNMT3A in osteoarthritis (PMID:31522815)
• Metformin reduces lipid accumulation in HepG2 cells via downregulation of miR-33b. (PMID:31686542)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
drosophila_melanogaster	mir-33	FBGN0262393

Paralogs (1): MIR33A (ENSG00000207932)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.