MIR365A

Summary

MIR365A (microRNA 365a, HGNC:33692) is a microRNA gene on chromosome 16p13.12.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126355 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33692
Approved symbol	MIR365A
Name	microRNA 365a
Location	16p13.12
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-365-1
Ensembl gene	ENSG00000199130
Ensembl biotype	miRNA
OMIM	614735
Entrez	100126355
RNAcentral	URS000075A669 — miRNA, 87 nt, 36 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362260

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362260 — 1 exons

Exon	Start	End
ENSE00001437023	14309285	14309371

Expression profiles

Bgee: expression breadth broad, 67 present calls, max score 87.14.

Top tissues by expression

67 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	87.14	gold quality
skeletal muscle tissue	UBERON:0001134	85.20	gold quality
lymph node	UBERON:0000029	77.78	gold quality
monocyte	CL:0000576	76.55	gold quality
muscle of leg	UBERON:0001383	76.55	gold quality
blood	UBERON:0000178	76.44	gold quality
placenta	UBERON:0001987	75.80	gold quality
stomach	UBERON:0000945	74.45	gold quality
tonsil	UBERON:0002372	73.93	gold quality
gastrocnemius	UBERON:0001388	73.36	gold quality
calcaneal tendon	UBERON:0003701	72.42	gold quality
body of pancreas	UBERON:0001150	71.22	gold quality
lung	UBERON:0002048	70.39	gold quality
adult mammalian kidney	UBERON:0000082	70.25	gold quality
corpus callosum	UBERON:0002336	70.08	gold quality
endometrium	UBERON:0001295	69.62	gold quality
Ammon’s horn	UBERON:0001954	69.04	gold quality
substantia nigra	UBERON:0002038	68.38	gold quality
omental fat pad	UBERON:0010414	68.38	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	68.28	gold quality
lower esophagus muscularis layer	UBERON:0035833	68.19	gold quality
ascending aorta	UBERON:0001496	68.03	gold quality
thoracic aorta	UBERON:0001515	67.80	gold quality
right lobe of liver	UBERON:0001114	67.69	gold quality
tibial artery	UBERON:0007610	67.51	gold quality
putamen	UBERON:0001874	67.10	gold quality
esophagus	UBERON:0001043	66.89	gold quality
esophagus mucosa	UBERON:0002469	66.21	gold quality
skin of abdomen	UBERON:0001416	66.05	gold quality
left adrenal gland	UBERON:0001234	65.97	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.32

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• Findings suggest that miR-193b-365 cluster is part of the unique miRNA signature in MM. (PMID:19883314)
• miR-365, a novel negative regulator of interleukin-6 gene expression, is cooperatively regulated by Sp1 and NF-kappaB (PMID:21518763)
• MiR-365 potentiates ox-LDL-induced endothelial cells apoptosis by regulating the expression of Bcl-2. (PMID:21640710)
• microRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2. (PMID:22072615)
• signaling loop between transforming growth factor beta (TGFb) and miR-365 and this loop reinforced suppression of TTF-1 via miR-365. (PMID:22185756)
• data suggests that miR-365 suppresses the expression and therefore the oncogenic functions of NKX2-1 in lung cancer (PMID:23507558)
• miR-365 may act as an onco-miR in cutaneous squamous cell carcinoma both in vitro and in vivo. (PMID:23514750)
• expression levels of selected candidate target genes (ELL, EBF3 and IRF4 for miR-24, PITPNC1 for miR-126 and ZAP-70 for miR-365) did not reduce upon miRNAs overexpression in MHH-CALL-3 TCF3-rearranged leukemic cells (PMID:24153013)
• MiR-365 directly targeted adaptor protein Src Homology 2 Domain Containing 1 (SHC1) and apoptosis-promoting protein BAX. (PMID:24216611)
• miR-193b/365a cluster controls progression of epidermal squamous cell carcinoma. (PMID:24374827)
• Further studies also discovered a conserved feedback regulatory circuitry formed by NFIB and miR-365 in cutaneous squamous cell carcinoma development (PMID:24949940)
• Our findings suggest that miR-365 expression was an independent poor prognostic factor for hepatocellular carcinoma patient overall survival and suppressed tumor cell growth. (PMID:25973057)
• Study show that miR365 expression is up-regulated in breast cancer cells and its inhibition reduces cell proliferation and cell invasion. (PMID:25998153)
• Aberrant expression of mir-365/TTF-1 may be involved in the tumor development in patients with non-small cell lung carcinoma. (PMID:26045746)
• MiR-365 can decrease migratory, invasive and proliferative behavior of malignant melanomas by attenuating the expression of NRP1. (PMID:26191184)
• Three miRNAs including hsa-mir-23b, hsa-mir-365-1 and hsa-mir-365-2 showed significant influence on prognosis of Colorectal cancer patients (PMID:26269151)
• Preoperative serum miR-365 and TTF-1 mRNA levels may be effective indicators of tumor aggressiveness in human NSCLC. miR-365 and its target gene TTF-1 appear to be synergistic risk factors for the reduction in overall survival of patients with NSCLC. (PMID:26337230)
• NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in malignant melanoma (PMID:26406949)
• Data show that microRNA miR-365 could inhibit the proliferation and promote the apoptosis in SOSP-9607 osteosarcoma cells probably by mediating the expression of KRAS protein. (PMID:26728377)
• Data suggest that miR-365a may act as an oncomiR and may promote growth and metastasis in laryngeal squamous cell carcinoma via the PI3K/AKT signaling pathway. (PMID:26883008)
• miR-365 was up-regulated by cyclic loading and IL-1beta stimulation in articular chondrocytes through a mechanism that involved the transcription factor NF-kappa B. (PMID:27023516)
• Our results indicate that miR-365 targets the HLA-G 3’ UTR to repress its expression. The expression of miR-365 may play an important role in human placental development and in immunoprotection of the semiallogenic embryo. (PMID:27577708)
• Results showed that miR-365 expression was significantly downregulated in glioma cell lines and tissues, and that miR-365 overexpression inhibited cell proliferation, migration and invasion of glioma by targeting PIK3R3. (PMID:28260020)
• Data show that miR-365 was significantly differentially expressed in recurrent miscarriage (RM) decidual tissues. (PMID:28393453)
• our study reveals that exosomes mediate a horizontal transfer of drug-resistant trait in chronic myeloid leukemia cell by delivering miR-365. (PMID:29223442)
• NKX2-1 expression was upregulated in never-smokers , lung adenocarcinoma and patients with wild-type TP53. A negative correlation between NKX2-1 and miR-365 expression was found but there was no correlation between NKX2-1 and miR-33a expression. NKX2-1 expression impacts prognosis in early-stage non-small cell lung cancer patients, particularly in those with neither TP53 nor KRAS mutations. (PMID:29237428)
• MiR-365 regulates IL-1beta-stimulated catabolic effects in human chondrocytes by modulating HIF-2alpha expression. (PMID:29263346)
• Circulating mir-365a-3p was positively correlated with HbA1c in Type 1 diabetics. (PMID:29453204)
• NEAT1 silencing also inhibited tumor growth in vivo. Collectively, we revealed that the NEAT1/miR365/RGS20 axis may be a novel mechanism or therapeutic strategy for OSCC treatment. (PMID:29484420)
• Low miR365 expression is associated with liver cancer. (PMID:30182377)
• TET1 is one of the targets of miR-365a-3p. miR-365a-3p regulates the biological behavior of laryngeal cancer by down-regulating TET1. (PMID:30249104)
• Targeting a novel miR-365-3p/EHF/KRT16/beta5-integrin/c-Met signaling pathway could improve treatment efficacy in OSCC. (PMID:30782177)
• Overexpression of miR-365 is able to enhance the radiosensitivity of non-small cell lung cancer (NSCLC) cells through targeting CDC25A. We found that the expression level of miR-365 was positively correlated with the radiosensitivity of NSCLC cell lines. (PMID:30902389)
• sulforaphane-induced miR-365a-3p expression inhibits NF-kappaB activity by downregulating c-Rel, which prevents the progression of pancreatic ductal adenocarcinoma (PMID:30910589)
• The decreased expression of miR-365 in human cervical cancer cells relieves its inhibitory effect on ELF4, which promotes the proliferation of cervical cancer cells and the formation of tumor. (PMID:31060678)
• miR-365 suppressed the expression of TRIM25 and elevated the expression of the proapoptotic protein in non-small-cell lung cancer cells. (PMID:31099423)
• circRNA_0058097 promotes tension-induced degeneration of endplate chondrocytes by regulating HDAC4 expression through sponge adsorption of miR-365a-5p. (PMID:31222836)
• Results indicate that miR-365 was both necessary and sufficient for activation of the marker genes for chondrogenesis, hypertrophy, and osteogenesis in the osteoarthritis patients mesenchymal stem cells (OA-MSC). (PMID:31287025)
• MiRNA-365a-3p promotes the progression of osteoporosis by inhibiting osteogenic differentiation via targeting RUNX2. (PMID:31599402)
• MiR-365/ADAM10 axis may present a new target for the treatment of breast cancer. (PMID:31786854)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mir365-1	ENSDARG00000098065
mus_musculus	Mir365-1	ENSMUSG00000065449
rattus_norvegicus	Mir365-1	ENSRNOG00000047135

Paralogs (1): MIR365B (ENSG00000283978)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.