MIR365B

Summary

MIR365B (microRNA 365b, HGNC:33693) is a microRNA gene on chromosome 17q11.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126356 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33693
Approved symbol	MIR365B
Name	microRNA 365b
Location	17q11.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-365-2
Ensembl gene	ENSG00000283978
Ensembl biotype	miRNA
Entrez	100126356
RNAcentral	URS000075E397 — miRNA, 111 nt, 23 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362317

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362317 — 1 exons

Exon	Start	End
ENSE00001437080	31575411	31575521

Expression profiles

Bgee: expression breadth broad, 43 present calls, max score 77.52.

Top tissues by expression

43 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
kidney	UBERON:0002113	77.52	gold quality
monocyte	CL:0000576	75.88	gold quality
blood	UBERON:0000178	73.70	gold quality
adrenal tissue	UBERON:0018303	73.13	gold quality
lymph node	UBERON:0000029	72.77	gold quality
liver	UBERON:0002107	72.45	gold quality
muscle layer of sigmoid colon	UBERON:0035805	70.87	gold quality
tibial nerve	UBERON:0001323	70.72	gold quality
body of stomach	UBERON:0001161	69.83	gold quality
body of pancreas	UBERON:0001150	69.80	gold quality
colon	UBERON:0001155	69.72	gold quality
stomach	UBERON:0000945	69.26	gold quality
heart	UBERON:0000948	69.23	gold quality
esophagus mucosa	UBERON:0002469	69.23	gold quality
heart left ventricle	UBERON:0002084	69.08	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	68.86	gold quality
omental fat pad	UBERON:0010414	67.92	gold quality
tibial artery	UBERON:0007610	67.63	gold quality
ascending aorta	UBERON:0001496	67.58	gold quality
right atrium auricular region	UBERON:0006631	67.55	gold quality
left lobe of thyroid gland	UBERON:0001120	67.43	gold quality
left ovary	UBERON:0002119	67.12	gold quality
left coronary artery	UBERON:0001626	66.82	gold quality
subcutaneous adipose tissue	UBERON:0002190	66.54	gold quality
right lobe of thyroid gland	UBERON:0001119	65.65	gold quality
skin of abdomen	UBERON:0001416	65.65	gold quality
zone of skin	UBERON:0000014	65.17	gold quality
endometrium	UBERON:0001295	65.14	gold quality
gastrocnemius	UBERON:0001388	64.40	gold quality
skin of leg	UBERON:0001511	64.31	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.08

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 15)

• Fndings suggest that miR-193b-365 cluster is part of the unique miRNA signature in MM. (PMID:19883314)
• Our findings suggest that miR-365 expression was an independent poor prognostic factor for hepatocellular carcinoma patient overall survival and suppressed tumor cell growth. (PMID:25973057)
• Study show that miR365 expression is up-regulated in breast cancer cells and its inhibition reduces cell proliferation and cell invasion. (PMID:25998153)
• Aberrant expression of mir-365/TTF-1 may be involved in the tumor development in patients with non-small cell lung carcinoma. (PMID:26045746)
• MiR-365 can decrease migratory, invasive and proliferative behavior of malignant melanomas by attenuating the expression of NRP1. (PMID:26191184)
• MIR365b-3p was differentially expressed in obese children with and without endothelial dysfunction. (PMID:26270249)
• Preoperative serum miR-365 and TTF-1 mRNA levels may be effective indicators of tumor aggressiveness in human NSCLC. miR-365 and its target gene TTF-1 appear to be synergistic risk factors for the reduction in overall survival of patients with NSCLC. (PMID:26337230)
• Results showed that miR-365 expression was significantly downregulated in glioma cell lines and tissues, and that miR-365 overexpression inhibited cell proliferation, migration and invasion of glioma by targeting PIK3R3. (PMID:28260020)
• Data show that miR-365 was significantly differentially expressed in recurrent miscarriage (RM) decidual tissues. (PMID:28393453)
• Overexpression of miR-365 is able to enhance the radiosensitivity of non-small cell lung cancer (NSCLC) cells through targeting CDC25A. We found that the expression level of miR-365 was positively correlated with the radiosensitivity of NSCLC cell lines. (PMID:30902389)
• miR-365b promoted hepatocellular carcinoma (HCC) cell motility and spreading. Furthermore, SGTB was found to be a downstream target of miR-365b, and knockdown of the SGTB gene could mimic the effect of miR-365b in hastening HCC cell migration and invasion. These results imply that miR-365b plays a tumor-promoting role in HCC by suppressing SGTB expression. (PMID:30943053)
• The decreased expression of miR-365 in human cervical cancer cells relieves its inhibitory effect on ELF4, which promotes the proliferation of cervical cancer cells and the formation of tumor. (PMID:31060678)
• Serum microRNA-365 suppresses non-small-cell lung cancer metastasis and invasion in patients with bone metastasis of lung cancer. (PMID:33121309)
• MicroRNA-365 induces apoptosis and inhibits invasion of human myeloma cells by targeting homeobox A9 (HOXA9). (PMID:33621688)
• Identification of hsa-miR-365b-5p’s role in Alzheimer’s disease: A combined analysis of miRNA and mRNA microarrays. (PMID:36181964)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	dre-mir-365-2	ENSDARG00000098708
danio_rerio	dre-mir-365-3	ENSDARG00000101273
rattus_norvegicus	Mir365b	ENSRNOG00000035655

Paralogs (1): MIR365A (ENSG00000199130)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.