MIR374B

Summary

MIR374B (microRNA 374b, HGNC:33665) is a microRNA gene on chromosome Xq13.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100126317 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33665
Approved symbol	MIR374B
Name	microRNA 374b
Location	Xq13.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-374b
Ensembl gene	ENSG00000212027
Ensembl biotype	miRNA
Entrez	100126317
RNAcentral	URS000075BF8B — miRNA, 72 nt, 22 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000390738

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000390738 — 1 exons

Exon	Start	End
ENSE00001508415	74218547	74218618

Expression profiles

Bgee: expression breadth tissue_specific, 3 present calls, max score 59.27.

Top tissues by expression

3 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal glomerulus	UBERON:0000074	59.27	silver quality
prostate gland	UBERON:0002367	54.22	gold quality
placenta	UBERON:0001987	50.81	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 27)

• Up-regulation of miR-374B is associated with triple negative breast cancer. (PMID:23405235)
• The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival. (PMID:24191917)
• Suggest that upregulation of miR-374b-5p contributes to gastric cancer cell metastasis and invasion through inhibition of RECK expression. (PMID:25516656)
• Report potential value of miR-1 and miR-374b as biomarkers for colorectal cancer at different stages. (PMID:26045793)
• MicroRNA-374b Suppresses Proliferation and Promotes Apoptosis in T-cell Lymphoblastic Lymphoma by Repressing AKT1 and Wnt-16 (PMID:26100275)
• we identified downregulation of miR-374b as likely being directly involved in acquisition of the drug-resistant phenotype. (PMID:27229158)
• Moreover, inhibition on miR-374b relieved the suppressed AKT1 protein, and reduced cell apoptosis induced by BLM. These data depict the p53/miR-374b/AKT1 signaling that may regulate BLM-induced apoptosis in Colorectal cancer (CRC)cells, thus facilitating to improve the outcome of chemotherapy in CRC. (PMID:28339062)
• It is a potential biomarker for amyotrophic lateral sclerosis. (PMID:28454844)
• Findings showed that miR-374b expression was significantly downregulated in colon cancer tissues and cell lines and suggest that miR-374b inhibits colon cancer cell proliferation and invasion through downregulation of LRH-1 expression. (PMID:29128635)
• iR-374b significantly inhibits cell proliferation, migration, and invasion through the blockade of the p38/ERK signaling pathway activation, as well as negatively binding to JAM-2 (PMID:29575013)
• Results showed that MiR-374b was highly expressed, while PTEN was downregulated in the GIST tissues. The levels of miR-374b, PI3K, AKT and PTEN were related to tumor diameter and pathological stage. Additionally, miR-374b increased the mRNA and protein levels of PI3K, Akt, MMP2, MMP9, P53 and cyclinD1, suggesting that miR-374b activates PI3K/Akt signaling pathway in GIST-T1 cells. (PMID:29902839)
• miR-374b-5p-FOXP1 feedback loop regulates tumor progression in ovarian cancer. (PMID:30274777)
• miR-454-3p and miR-374b-5p negatively regulated invasion and migration of Bladder cancer cell lines via targeting ZEB2. (PMID:30352837)
• MiR-374b reduces cell proliferation and cell invasion of cervical cancer through regulating FOXM1. (PMID:30720158)
• a functional MAGI2-AS3/miR-374b-5p/SMG1 axis that suppresses hepatocellular cancer progression, is reported. (PMID:30924168)
• Diagnostic Value of Peripheral Blood miR-374b-5p in Patients with Prostate Cancer. (PMID:32013356)
• Differential microRNAs expression in seminal plasma of normospermic patients with different sperm DNA fragmentation indexes. (PMID:32259568)
• MicroRNA-374b mediates the initiation of non-small cell lung cancer by regulating ITGB1 and p53 expressions. (PMID:32364676)
• ABCA8 is regulated by miR-374b-5p and inhibits proliferation and metastasis of hepatocellular carcinoma through the ERK/ZEB1 pathway. (PMID:32430024)
• miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10. (PMID:32445843)
• Circulating miR-374b-5p negatively regulates osteoblast differentiation in the progression of osteoporosis via targeting Wnt3 AND Runx2. (PMID:32548991)
• MicroRNA-374b inhibits breast cancer progression through regulating CCND1 and TGFA genes. (PMID:33480984)
• hsa-miR-374b-5p regulates expression of the gene U2AF homology motif (UHM) kinase 1. (PMID:34160076)
• The role of circulating blood microRNA-374 and microRNA-10 levels in the pathogenesis and therapeutic mechanisms of major depressive disorder. (PMID:34418506)
• MiRNA-374b-5p and miRNA-106a-5p are related to inflammatory bowel disease via regulating IL-10 and STAT3 signaling pathways. (PMID:36437465)
• MAGI2-AS3 and miR-374b-5p as Putative Regulators of Multiple Sclerosis via Modulating the PTEN/AKT/IRF-3/IFN-beta Axis: New Clinical Insights. (PMID:36878000)
• Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis. (PMID:37691099)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir374b	ENSMUSG00000076269

Paralogs (1): MIR374A (ENSG00000199168)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.