MIR409

Summary

MIR409 (microRNA 409, HGNC:32055) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574413 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32055
Approved symbol	MIR409
Name	microRNA 409
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-409
Ensembl gene	ENSG00000199107
Ensembl biotype	miRNA
OMIM	614057
Entrez	574413
RNAcentral	URS0000759A5D — miRNA, 79 nt, 34 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362237

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362237 — 1 exons

Exon	Start	End
ENSE00001437000	101065300	101065378

Expression profiles

Bgee: expression breadth broad, 84 present calls, max score 95.48.

Top tissues by expression

84 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	95.48	gold quality
placenta	UBERON:0001987	88.73	gold quality
lung	UBERON:0002048	80.68	gold quality
kidney	UBERON:0002113	79.33	gold quality
left adrenal gland cortex	UBERON:0035825	78.59	gold quality
adrenal gland	UBERON:0002369	77.25	gold quality
stomach	UBERON:0000945	76.16	gold quality
muscle of leg	UBERON:0001383	75.98	gold quality
left ovary	UBERON:0002119	75.81	gold quality
left adrenal gland	UBERON:0001234	75.41	gold quality
tonsil	UBERON:0002372	74.90	gold quality
ovary	UBERON:0000992	74.41	gold quality
blood	UBERON:0000178	74.35	gold quality
right adrenal gland	UBERON:0001233	74.15	gold quality
monocyte	CL:0000576	73.87	gold quality
endometrium	UBERON:0001295	73.52	gold quality
body of stomach	UBERON:0001161	73.27	gold quality
right adrenal gland cortex	UBERON:0035827	73.22	gold quality
gastrocnemius	UBERON:0001388	73.06	gold quality
gall bladder	UBERON:0002110	72.99	gold quality
vagina	UBERON:0000996	72.87	gold quality
body of pancreas	UBERON:0001150	72.79	gold quality
adult mammalian kidney	UBERON:0000082	72.72	gold quality
islet of Langerhans	UBERON:0000006	72.61	gold quality
myometrium	UBERON:0001296	71.53	gold quality
muscle layer of sigmoid colon	UBERON:0035805	71.22	gold quality
colon	UBERON:0001155	70.95	gold quality
intestine	UBERON:0000160	70.92	gold quality
right atrium auricular region	UBERON:0006631	70.85	gold quality
omental fat pad	UBERON:0010414	70.45	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): PHF10

Literature-anchored findings (GeneRIF, showing 40)

• Data show that silencing of radixin (RDX) phenocopied the effects of miR-409 overexpression, whereas restoration of RDX in miR-409-overexpressed gastric cancer (GC) cells reversed the suppressive effects of miR-409. (PMID:22179828)
• these findings suggest that miR-409-3p may function as a novel tumor suppressor in GC and its anti-oncogenic activity may involve the direct targeting and inhibition of PHF10. (PMID:22388101)
• data demonstrate that miR-409-3p inhibits tumor growth, vascularization and metastasis through down-regulating ANG expression (PMID:22531314)
• Upregulation of plasma MIR409-3p is associated with breast cancer. (PMID:22927033)
• In primary immune thrombocytopenia patients, decreased DGCR8 leads to down-regulation of MIR409-3p, which in turn results in up-regulation of IFN-gamma. (PMID:23360331)
• The pro-metastatic gene c-Met as a potential miR-409-3p target. (PMID:23820886)
• This finding bears particular translational importance as miR-409-3p/-5p appears to be an attractive biomarker and/or possibly a therapeutic target to treat bone metastatic prostate cancer (PMID:24963047)
• Stromal fibroblasts derived miR-409-induced tumorigenesis. (PMID:25065597)
• MiR-409-3p expression was an independent prognostic factor for LAD patients. (PMID:25278243)
• Data demonstrate that miR-409-3p is a metastatic suppressor, and post-transcriptional inhibition of the oncoprotein GAB1 is one of its mechanisms of action. (PMID:25991585)
• miR-409-3p functions as a tumor suppressor. (PMID:26084278)
• Our data collectively indicate that miR-409-3p functions as a tumor suppressor in BC through downregulating Akt1. (PMID:26631969)
• The present study provides evidence that miR-409-3p targets and inhibits Beclin-1, which inhibits chemotherapy-induced autophagy and thereby enhances the chemosensitivity of colon cancer cells. (PMID:26935807)
• expression of catenin-delta1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion (PMID:26992637)
• Our results confirmed miR-181d-5p as the key MGMT-regulating miRNA in glioblastoma and miR-409-3p modifies this effect (PMID:27057640)
• miR-409-3p can regulate the invasion and metastasis process of breast cancer by targeting ZEB1. (PMID:27079864)
• The tissue RASGRP3 mRNA, target gene of miR-409-3p, was significantly up-regulated in the Mitral regurgitation patients with Heart failure compared to normal controls . (PMID:27505319)
• miR-409-3p was related to the prognosis of patients with breast cancer and might be a promising predictor of miR-409-3p recurrence (PMID:27735035)
• We observed decreased miR-409-3p expression in glioma, and overexpression of miR-409-3p repressed glioma cell invasion and proliferation by targeting HMGN5, an oncogene associated with glioma (PMID:28109076)
• Ras suppressor protein 1 was confirmed to be directly targeted by miR-409-5p in breast cancer cells. Small interfering RNA-mediated Ras suppressor protein 1 inhibition reversely promoted cancer proliferation and migration in miR-409-5p-downregualted breast cancer cells. MiR-409-5p is downregulated in breast cancer and its inhibition has anti-cancer effect on breast cancer development both in vitro and in vivo. (PMID:28459205)
• The microRNA-409-3p level was lower in cervical carcinoma tissues, and significantly affected the overall survival, tumor size and TNM staging in clinical patients; Low expression of microRNA-409-3p could promote the proliferative ability of cervical carcinoma cells through the target gene AKT. (PMID:29509241)
• these results indicated that PDK1 facilitated clear cell renal cell carcinoma cell glycolysis, regulated by miR-409-3p in hypoxia. (PMID:30218446)
• Low miR409 expression is associated with endometrial carcinoma. (PMID:30431090)
• ZEB1-AS1/miR-409-3p/ZEB1 constitutes a positive feedback loop to promote the tumorigenesis of non-small cell lung cancer (PMID:30448056)
• Compared with paracancerous and normal liver tissues, the level of microRNA-409 was remarkably reduced in hepatocarcinoma tissues and was negatively correlated with tumor stage, tumor size and overall survival time of patients with liver cancer. Meanwhile, microRNA-409 expression in hepatoma cell lines was also strikingly lower than that in normal liver cells. (PMID:30657556)
• Investigation revealed an inverse correlation of miR-409-3p with E6 mRNA levels in precancerous cervical lesions. (PMID:30711417)
• MiRNA-409-5p dysregulation promotes imatinib resistance and disease progression in children with chronic myeloid leukemia. (PMID:31646577)
• The Expression and Clinical Significance of MicroRNA-409-3p in Acute Myeloid Leukemia. (PMID:32538052)
• Circ_0000745 Promotes the Progression of Cervical Cancer by Regulating miR-409-3p/ATF1 Axis. (PMID:32644859)
• Long noncoding RNA PSMA3AS1 functions as a microRNA4093p sponge to promote the progression of nonsmall cell lung carcinoma by targeting spindlin 1. (PMID:32945481)
• MicroRNA-409-3p promotes osteoblastic differentiation via activation of Wnt/beta-catenin signaling pathway by targeting SCAI. (PMID:33274736)
• miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10. (PMID:33287942)
• Plasma miR-409-3p promotes acute cerebral infarction via suppressing CTRP3. (PMID:33336518)
• Plasma Exosomal CircNEK9 Accelerates the Progression of Gastric Cancer via miR-409-3p/MAP7 Axis. (PMID:33449227)
• Precision hyperthermia-induced miRNA-409-3p upregulation inhibits migration, invasion, and EMT of gastric cancer cells by targeting KLF17. (PMID:33667708)
• miRNAs derived from circulating small extracellular vesicles as diagnostic biomarkers for nasopharyngeal carcinoma. (PMID:33728743)
• CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis. (PMID:33818788)
• MicroRNA-409-3p regulates macrophage migration in polymyositis through targeting CXCR4. (PMID:34142881)
• The clinical significance of microRNA-409 in pancreatic carcinoma and associated tumor cellular functions. (PMID:34338153)
• Exercise as a model to identify microRNAs linked to human cognition: a role for microRNA-409 and microRNA-501. (PMID:34625536)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir409	ENSMUSG00000105849
mus_musculus	Gm54371	ENSMUSG00002075156

Paralogs (16): MIR494 (ENSG00000194717), MIR377 (ENSG00000199015), MIR381 (ENSG00000199020), MIR369 (ENSG00000199025), MIR323A (ENSG00000199069), MIR410 (ENSG00000199092), MIR539 (ENSG00000202560), MIR487A (ENSG00000207742), MIR487B (ENSG00000207754), MIR656 (ENSG00000207959), MIR496 (ENSG00000207961), MIR154 (ENSG00000207978), MIR323B (ENSG00000208004), MIR1185-1 (ENSG00000221525), MIR1185-2 (ENSG00000221614), MIR382 (ENSG00000283170)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.