MIR424

Summary

MIR424 (microRNA 424, HGNC:31881) is a microRNA gene on chromosome Xq26.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 494336 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31881
Approved symbol	MIR424
Name	microRNA 424
Location	Xq26.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-424
Ensembl gene	ENSG00000284231
Ensembl biotype	miRNA
OMIM	300682
Entrez	494336
RNAcentral	URS000075BC52 — miRNA, 98 nt, 14 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362227

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362227 — 1 exons

Exon	Start	End
ENSE00001436990	134546614	134546711

Expression profiles

Bgee: expression breadth broad, 28 present calls, max score 94.18.

Top tissues by expression

28 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal glomerulus	UBERON:0000074	94.18	gold quality
placenta	UBERON:0001987	82.42	gold quality
gastrocnemius	UBERON:0001388	73.22	gold quality
muscle layer of sigmoid colon	UBERON:0035805	70.39	gold quality
blood	UBERON:0000178	70.30	gold quality
omental fat pad	UBERON:0010414	69.88	gold quality
transverse colon	UBERON:0001157	69.07	gold quality
epididymis	UBERON:0001301	68.95	gold quality
tibial artery	UBERON:0007610	67.56	gold quality
endometrium	UBERON:0001295	67.29	gold quality
uterus	UBERON:0000995	67.28	gold quality
right ovary	UBERON:0002118	67.15	gold quality
ovary	UBERON:0000992	66.90	gold quality
left ovary	UBERON:0002119	66.84	gold quality
adenohypophysis	UBERON:0002196	66.27	gold quality
vagina	UBERON:0000996	65.38	gold quality
pituitary gland	UBERON:0000007	64.05	gold quality
islet of Langerhans	UBERON:0000006	63.59	gold quality
right hemisphere of cerebellum	UBERON:0014890	61.51	gold quality
tibial nerve	UBERON:0001323	60.49	gold quality
anterior cingulate cortex	UBERON:0009835	60.49	gold quality
thyroid gland	UBERON:0002046	60.44	gold quality
cerebellar hemisphere	UBERON:0002245	60.05	gold quality
right lobe of thyroid gland	UBERON:0001119	58.19	gold quality
prostate gland	UBERON:0002367	55.65	gold quality
testis	UBERON:0000473	52.08	gold quality
spleen	UBERON:0002106	51.11	gold quality
left testis	UBERON:0004533	50.68	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.49

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 40)

• MUC1 and galectin-3 function as part of a miR-322-dependent regulatory loop. (PMID:17889671)
• These findings indicate that MUC1 and galectin-3 function as part of a miR-322-dependent regulatory loop. (PMID:17889671)
• a significant regulation of PLAG1 by miR-181a, miR-181b, miR-107, and miR-424. (PMID:19692702)
• Results reveal an unexpected role of Cdc25A down-regulation and the inhibitory phosphorylation of cdk2 T14 and Y15 in cell cycle quiescence during muscle differentiation and implicate miRNAs-322 and -503 in the process. (PMID:20462953)
• These results suggest that miR-322/424 plays an important physiological role in post-ischemic vascular remodeling and angiogenesis, and regulate HIF-1alpha isoforms.. (PMID:20972335)
• decreased mir-424 expression and increased levels of MEK1 or cyclin E1 in senile hemangioma may cause abnormal cell proliferation in the tumor (PMID:21179471)
• MicroRNA-16 and microRNA-424 regulate cell-autonomous angiogenic functions in endothelial cells via targeting vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1. (PMID:21885851)
• miR-424 over-expression is an effective factor in maturation of the monoblastic U937 cells and it has the ability of directing them into cells, expressing monocyte/macrophage characteristics. (PMID:21902894)
• Results identify a crucial tumor suppressive role of miR-424 in the progression of cervical cancer at least partly via upreglating the expression of Chk1. (PMID:22469983)
• miR-424 was down-modulated in acute myeloid leukaemia’s with NPM1 mutation. (PMID:22681934)
• miR-503 was downregulated in pulmonary arterial hypertension, exerted antiproliferative effects in pulmonary artery endothelial cells (PMID:23263626)
• Demonstrate that miR-424/322 is up-regulated after vascular injury. This is likely an adaptive response to counteract smooth muscle cell proliferation. (PMID:23447642)
• the increased levels of miRNA-424 were negatively correlated with the mRNA levels of MEK1 in placentae complicated with fetal growth restriction (FGR); study suggests upregulated miRNA-424 may be associated with the pathogenesis of FGR (PMID:23643257)
• HDACs and NF-kB signaling coordinate epithelial expression of CX3CL1 to promote mucosal antimicrobial defense through suppression of the mir-424-503 gene. (PMID:23724129)
• MiR-424 was significantly upregulated in FXTAS patients. (PMID:23790110)
• Authors hypothesize that miR-424 promotes monocytic differentiation by regulating other critical factors and miR-424 has high affinity for these factors. (PMID:23801117)
• we show that miR-424 is uniquely downregulated in primary human trophoblasts by hypoxia or chemicals known to hinder cell differentiation. We also identify FGFR1 as a direct target of miR-424 in human trophoblasts. (PMID:23803556)
• By receiver-operator curve analysis of hair shaft miR-424 to distinguish psoriatic patients from normal subjects, the area under the curve was 0.77. However, relative miR-424 levels were not correlated with disease activity markers. (PMID:24628460)
• Recurrent microdeletion was detected in Xq26.3, causing loss of PHF6 expression, a potential tumor suppressor gene, and the miR-424, which is involved in the development of acute myeloid leukemia. (PMID:24674452)
• miR-424 or miR-503 mediate anti-proliferative and anti-invasive actions of thyroid hormone receptor beta (PMID:24796297)
• Overall, our findings demonstrate a novel mechanism, mediated by elevated expression of the miR424-503 cluster, underlying TGFbeta activation and metastasis of human breast cancer. (PMID:25164015)
• Aberrant expression of miR-424-5p is critically involved in resistance to anoikis. (PMID:25175916)
• miR-424(322)/503-dependent posttranscriptional downregulation of CDC25A cooperates with transcriptional repression of the CDC25A promoter and proteasome-mediated degradation to reduce the levels of CDC25A expression and to induce cell cycle arrest. (PMID:25266660)
• miR-424 regulates the myofibroblast differentiation during epithelial-to-mesenchymal transition by potentiating the TGF-beta signaling pathway, likely through Smurf2. (PMID:25524739)
• miR-424 is downregulated in Pulmonary arterial hypertension as well as apelin. Restoration of apelin and miR-424 levels reduces Pulmonary arterial hypertension severity. (PMID:25660363)
• Low expression of miR-424 is associated with chronic lymphocytic leukemia. (PMID:25670628)
• Findings indicate that miR-424 targets the E2F7 transcript and suppresses endometrial cancer cell growth, suggesting that miR-424 has a tumor suppressive role in human endometrial cancer pathogenesis. (PMID:25708247)
• Breast cancer patient miR-424 levels positively associate with TWIST1/2. (PMID:25716682)
• DNMT1 has a role in suppressing microRNA424 regulation of tumor progression in human bladder cancer (PMID:26090723)
• mirn424 has a role in inhibiting Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma (PMID:26315541)
• let-7c, miR-200b, miR-222 and miR-424 target PBX3, which is necessary for the acquisition and maintenance of liver tumour-initiating cells properties. (PMID:26420065)
• Data suggest microRNA-424 regulates expression of MGAT4A (mannoside beta-1,4-N-acetylglucosaminyltransferase A), OGT (O-linked N-acetylglucosamine transferase), and GALNT13 (polypeptide N-acetylgalactosaminyltransferase 13) in mammary epithelium. (PMID:26589799)
• Mir-424 plays a role in endometrial cancer cells proliferation via targeting GPER expression. (PMID:26638889)
• found a significant systematic difference in expression levels between tumor zones for miR-92a, miR-424 and miR-375 (PMID:26681654)
• miR-424 interacts with long non-coding RNA OIP5-AS1 and competes with HuR for binding to OIP5-AS1. (PMID:26819413)
• Our findings provide the compelling evidence that the decreased expression of serum miR-424 may serve as a novel biomarker to predict the unfavorable prognosis of hepatocellular carcinoma patients. (PMID:26823812)
• In this study, we detected relatively lower expression levels of miR-424 & mir- 27a in TRAIL-resistant and semi-resistant acute myeloid leukemia cell lines as well as newly diagnosed patient samples (PMID:27013583)
• miR-424-5p expression in oral squamous cell carcinoma cells could be induced by IL-8 through increasing STAT5 transcriptional activity. (PMID:27038552)
• MiR-424 regulates the PD-L1/PD-1 and CD80/CTLA-4 pathways in chemoresistant ovarian cancer. (PMID:27147225)
• Results suggest that E7 recruited CUL2, driven by CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates HPV16-induced cervical carcinogenesis. (PMID:27153550)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir322	ENSMUSG00000065418

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.