MIR433

Summary

MIR433 (microRNA 433, HGNC:32026) is a microRNA gene on chromosome 14q32.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574034 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32026
Approved symbol	MIR433
Name	microRNA 433
Location	14q32.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-433
Ensembl gene	ENSG00000207569
Ensembl biotype	miRNA
OMIM	611711
Entrez	574034
RNAcentral	URS0000688CFC — miRNA, 93 nt, 36 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384837

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384837 — 1 exons

Exon	Start	End
ENSE00001499844	100881886	100881978

Expression profiles

Bgee: expression breadth broad, 17 present calls, max score 69.36.

Top tissues by expression

17 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	69.36	gold quality
Ammon’s horn	UBERON:0001954	66.51	gold quality
substantia nigra	UBERON:0002038	66.38	gold quality
midbrain	UBERON:0001891	66.37	gold quality
esophagus mucosa	UBERON:0002469	66.18	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	63.73	gold quality
blood	UBERON:0000178	63.13	gold quality
left ovary	UBERON:0002119	61.57	gold quality
ovary	UBERON:0000992	61.56	gold quality
left adrenal gland	UBERON:0001234	60.33	gold quality
left adrenal gland cortex	UBERON:0035825	59.57	gold quality
left lobe of thyroid gland	UBERON:0001120	59.37	gold quality
skin of leg	UBERON:0001511	58.92	gold quality
cerebellar hemisphere	UBERON:0002245	57.18	gold quality
spleen	UBERON:0002106	57.16	gold quality
nucleus accumbens	UBERON:0001882	56.48	gold quality
pituitary gland	UBERON:0000007	53.54	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ESRRG, NR0B2, SMAD2, SMAD3

Literature-anchored findings (GeneRIF, showing 40)

• Down-regulation of miR-433 is associated with gastric carcinoma. (PMID:19531230)
• the miR-433/127 loci may have evolved from a common gene of origin in mammals (PMID:19946636)
• HDAC6 3’-UTR variant suppressed hsa-miR-433-mediated post-transcriptional regulation causing the overexpression of HDAC6. (PMID:20181727)
• The common FGF20 rs12720208 SNP was not associated with the risk for Parkinson’s disease (PD) in our population. In addition, we did not find nucleotide changes in miR-433 (that binds to the 3’ UTR FGF20 mRNA) among our PD patients. (PMID:20471450)
• Upregulation of MIR-433 is associated with myeloproliferative neoplasms. (PMID:22864358)
• The ectopic expression of miR-433 and mir-127 in gastric cancer cell lines inhibits cell proliferation, cell migration and invasion by interacting with the mRNA encoding oncogenic factors KRAS and MAPK4 respectively. (PMID:23880861)
• Low mirn433 expression is associated with resistance to 5-fluorouracil in cancer. (PMID:23915286)
• A miR-433 seed match region in human and mouse CREB1 3’-UTRs, was identified. (PMID:23979134)
• SNP1599 differentially regulates osteonectin expression and contributes to variability in bone mass, by a mechanism that may involve differential targeting by miR-433 (PMID:25262637)
• miR-433 targets both catalytic (GCLc) and regulatory (GCLm) subunits of GCL. (PMID:25353619)
• microRNA-433 (miRNA-433 directly targets PAK4 through the miRNA-433 binding sequence at the 3’-UTR of PAK4 mRNA. (PMID:25410752)
• Our data highlight how the aberrant expression of miR-433 can adversely affect intracellular signaling to mediate chemoresistance in OC cells by driving cellular senescence. (PMID:25684390)
• miR-433 could be one of the vital senescence-associated miRNAs of HDPCs and found its target (GRB2), validated that miR-433 could negatively regulate GRB2 and the RAS-MAPK signaling pathway (PMID:25778413)
• The findings indicate that miR-433 may inhibit cell migration and invasion in the development of ovarian cancer via down-regulation of Notch1. (PMID:27468873)
• restoration of Notch1 and PAX6 expression partially rescued the inhibition of cell proliferation and metastasis induced by miR-433 overexpression. (PMID:27470361)
• This study highlights that miR-433 represses nonsense mediated mRNA decay. The miR-433 targets 3’-UTR of SMG5 and represses the expression of SMG5, whereas nonsense-mediated mRNA decay activity is decreased when SMG5 is decreased. (PMID:27473591)
• MIR433 targets the 3’-UTR of HIF-1alpha and inhibits proliferation and migration of HUVEC cells. (PMID:27815672)
• Data reveal that miR-433 is downregulated in glioma tissue and cells, and functions as a tumor suppressor by targeting CREB in glioma, thus regulating cell growth, invasion and migration. (PMID:27926502)
• MiR-433-3p siRNA decreased luminescence signal, indicating direct regulation of miR-433-3p on DKK1 mRNA. When the miR-433-3p binding site in DKK1 3’UTR was mutant, such reduction was prohibited. (PMID:28628652)
• Study demonstrated that miR433 is significantly downregulated in cervical cancer, and low expression level of this miRNA is associated with tumour size, FIGO stage, lymph node and distant metastases. In vitro studies demonstrated that miR-433 suppressed cellular proliferation and invasion and increased apoptosis in cervical cancer cells by directly targeting metadherin. (PMID:29130111)
• that miR-433 was frequently downregulated in colon cancer tissues and cell lines. Overexpression of miR-433 significantly inhibited the proliferation and invasion of colon cancer. We also newly identified HOXA1 as a direct target of miR-433. The effects of miR-433 on colon cancer cells were mediated via HOXA1. (PMID:29137689)
• Down-regulated Exportin-5 impairs the nuclear export of miR-433 and miR-22 precursor forms, causing a decrease in levels of mature miR-433 and miR-22 forms, and leading to overexpression of HDAC6 and ciliary loss in cholangiocarcinoma (PMID:29406621)
• The binding site of miR-433-3p was identified in the 3’UTR region of GRB2. Western blotting and FQ-PCR showed that miR-433-3p inhibited the mRNA and protein expression of GRB2. (PMID:29730656)
• a significant association between a functional polymorphism in the FGF20 gene, which regulates its modulation by miR-433, and depressive symptoms, is reported. (PMID:30241547)
• Study showed that the expression of miRNA433 was downregulated in patients with cervical cancer and correlated with poor survival. Its upregulation suppressed the growth and induted the apoptosis of cervical cancer cells through FAK/PI3K/AKT signaling. These results revealed that miRNA433 suppressed the growth of cervical cancer cells via the FAK/PI3K/AKT. (PMID:30272334)
• This study demonstrated the CREB1/miR-433 reciprocal feedback loop restrained the propagation, invasion and metastasis activities of CRC cells through abrogation of cell cycle progression and constraint of EMT. (PMID:30523220)
• Results show that miR-433 expression is regulated by Circ-ATP8A2 through its sponging in cervical cancer. (PMID:31029604)
• Circular RNA circ_0079593 indicates a poor prognosis and facilitates cell growth and invasion by sponging miR-182 and miR-433 in glioma. (PMID:31148222)
• Here, we show that miR-433 directly binds to Smad2, which is shown to be upregulated in non-small cell lung carcinomas. miR-433 expression is downregulated in NSCLC tissues and cells. Overexpression of miR-433 is associated with decreased expression of proteins - namely Cyclin D1, MMP-2/TIMP-2, and MMP-9, and consequently reduced cell proliferation and invasion phenotypes. (PMID:31445716)
• Long noncoding RNA SNHG14 promotes osteosarcoma progression via miR-433-3p/FBXO22 axis. (PMID:31948764)
• LncRNA PCGEM1 promotes renal carcinoma progression by targeting miR-433-3p to regulate FGF2 expression. (PMID:31958075)
• MiR-433 Regulates Myocardial Ischemia Reperfusion Injury by Targeting NDRG4 Via the PI3K/Akt Pathway. (PMID:32187107)
• Overexpression of miRNA-433-5p protects acute spinal cord injury through activating MAPK1. (PMID:32271400)
• Alteration of miR-21, miR-433 and miR-590 tissue expression related to the TGF-beta signaling pathway in ulcerative colitis patients. (PMID:32412349)
• LncRNA GNAS-AS1 facilitates ER+ breast cancer cells progression by promoting M2 macrophage polarization via regulating miR-433-3p/GATA3 axis. (PMID:32538432)
• Long noncoding RNA OSER1AS1 promotes the malignant properties of nonsmall cell lung cancer by sponging microRNA4333p and thereby increasing Smad2 expression. (PMID:32627026)
• LncRNA PCGEM1 accelerates non-small cell lung cancer progression via sponging miR-433-3p to upregulate WTAP. (PMID:32787827)
• Clinical significance of miR-433 in the diagnosis of Alzheimer’s disease and its effect on Abeta-induced neurotoxicity by regulating JAK2. (PMID:32871216)
• Serum miRNA125a-5p, miR-125b-5p, and miR-433-5p as biomarkers to differentiate between posterior circulation stroke and peripheral vertigo. (PMID:33038923)
• HIF-1alpha downregulation of miR-433-3p in adipocyte-derived exosomes contributes to NPC progression via targeting SCD1. (PMID:33511729)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir433	ENSMUSG00000070072
rattus_norvegicus	Mir433	ENSRNOG00000036497

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.