MIR4443

Summary

MIR4443 (microRNA 4443, HGNC:41830) is a microRNA gene on chromosome 3p21.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100616407 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:41830
Approved symbol	MIR4443
Name	microRNA 4443
Location	3p21.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-4443
Ensembl gene	ENSG00000265483
Ensembl biotype	miRNA
Entrez	100616407
RNAcentral	URS0000759B7F — miRNA, 53 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000585052

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000585052 — 1 exons

Exon	Start	End
ENSE00002723125	48196564	48196616

Expression profiles

Bgee: expression breadth broad, 16 present calls, max score 83.14.

Top tissues by expression

16 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right frontal lobe	UBERON:0002810	83.14	gold quality
gastrocnemius	UBERON:0001388	72.83	gold quality
monocyte	CL:0000576	72.76	gold quality
lower esophagus muscularis layer	UBERON:0035833	70.66	gold quality
Brodmann (1909) area 9	UBERON:0013540	68.93	gold quality
subcutaneous adipose tissue	UBERON:0002190	66.31	gold quality
lung	UBERON:0002048	66.09	gold quality
thyroid gland	UBERON:0002046	65.78	gold quality
left lobe of thyroid gland	UBERON:0001120	64.42	gold quality
tibial nerve	UBERON:0001323	63.71	gold quality
muscle layer of sigmoid colon	UBERON:0035805	63.68	gold quality
pituitary gland	UBERON:0000007	60.32	gold quality
colon	UBERON:0001155	59.65	gold quality
testis	UBERON:0000473	50.69	gold quality
left testis	UBERON:0004533	50.30	gold quality
right testis	UBERON:0004534	48.60	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 18)

• GLP-1 promoted adipogenesis through the modulation of the Wnt4/beta-catenin signaling pathway (PMID:27504971)
• miR-4443 acts in a tumor-suppressive manner by down-regulating TRAF4 and NCOA1 downstream of MEK-C/EBP-mediated leptin and insulin signaling, and that insulin and/or leptin resistance (e.g. in obesity) may suppress this pathway and increase the risk of metastatic CRC. (PMID:27842582)
• results support a scenario by which T cell-derived microvesicles act as intercellular carriers of functional miR-4443, which might exert heterotypic regulation of PTPRJ gene expression in mast cells (PMID:28823811)
• MNX1-AS1 promoted the proliferation, migration, and invasion of GBM cells through inhibiting miR-4443. (PMID:29678219)
• Overexpression of miR-4443 may promote the resistance of non-small cell lung cancer cells to epirubicin by targeting INPP4A and regulating the activation of JAK2/STAT3 pathway (PMID:30001772)
• the results of the present study demonstrated that FEZF1-AS1 promoted the proliferation, migration and invasion of HCC cells by regulating miR-4443 expression levels. The present results highlight the importance of the FEZF1-AS1/miR-4443 axis during HCC development and progression. (PMID:30365146)
• miR-4443 and miR-5195-3p can be considered tumor suppressors, and reductions in their expression can be associated with increased tumorigenicity and cell invasion. (PMID:30810880)
• Microenvironment-induced TIMP2 loss by cancer-secreted exosomal miR-4443 promotes liver metastasis of breast cancer. (PMID:31970775)
• Leptin-Responsive MiR-4443 Is a Small Regulatory RNA Independent of the Canonic MicroRNA Biogenesis Pathway. (PMID:32069948)
• MicroRNA-4443 regulates monocyte activation by targeting tumor necrosis factor receptor associated factor 4 in stroke-induced immunosuppression. (PMID:32337817)
• Long Noncoding RNA LINC00460 Promotes Cell Progression by Sponging miR-4443 in Head and Neck Squamous Cell Carcinoma. (PMID:32478564)
• [MiR-4443 promotes migration and invasion of breast cancer cells by inhibiting PEBP1 expression]. (PMID:33380387)
• Exosomal miR-4443 promotes cisplatin resistance in non-small cell lung carcinoma by regulating FSP1 m6A modification-mediated ferroptosis. (PMID:33781830)
• miR-4443 targets TRIM14 to suppress metastasis and energy metabolism of papillary thyroid carcinoma (PTC) in vitro. (PMID:34051007)
• Aberrant expression of microRNA-4443 (miR-4443) in human diseases. (PMID:36250718)
• miR-4443 promotes radiation resistance of esophageal squamous cell carcinoma via targeting PTPRJ. (PMID:36578050)
• Upregulated expression of miR-4443 and miR-4488 in drug resistant melanomas promotes migratory and invasive phenotypes through downregulation of intermediate filament nestin. (PMID:38008717)
• miR-4443 Contained Extracellular Vesicles: A Factor for Endometriosis Progression by PI3K/AKT/ACSS2 Cascade in-vitro. (PMID:38911502)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.