MIR448

Summary

MIR448 (microRNA 448, HGNC:26069) is a microRNA gene on chromosome Xq23.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 554212 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26069
Approved symbol	MIR448
Name	microRNA 448
Location	Xq23
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-448
Ensembl gene	ENSG00000199001
Ensembl biotype	miRNA
OMIM	300686
Entrez	554212
RNAcentral	URS00006EA8E0 — miRNA, 111 nt, 10 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000362131

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000362131 — 1 exons

Exon	Start	End
ENSE00001436894	114823454	114823564

Expression profiles

Bgee: expression breadth tissue_specific, 9 present calls, max score 87.08.

Top tissues by expression

9 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
blood	UBERON:0000178	87.08	gold quality
gastrocnemius	UBERON:0001388	72.93	gold quality
muscle layer of sigmoid colon	UBERON:0035805	71.33	gold quality
tibial nerve	UBERON:0001323	67.14	gold quality
skin of abdomen	UBERON:0001416	66.13	gold quality
omental fat pad	UBERON:0010414	65.73	gold quality
lower esophagus muscularis layer	UBERON:0035833	64.62	gold quality
skin of leg	UBERON:0001511	55.49	gold quality
left testis	UBERON:0004533	42.81	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.22

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 37)

• We demonstrated that pterostilbene mediated NFkappaB downregulation was correlated to an increased amount of microRNA 448 so pterostilbene effectively suppresses the generation of cancer stem cells. (PMID:23504987)
• miR-448 may contribute to the progression of Hepatocellular Carcinoma via regulating ROCK2 expression. (PMID:25969175)
• Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12. (PMID:26103953)
• The miRNA-448 and 4478 were significantly overexpressed in lung cancer patients (PMID:26341493)
• Downregulation of miR448 is associated with T-cell acute lymphoblastic leukemia. (PMID:26882564)
• Hsa-miR-448 disrupting KDM5B-MALAT1 signalling axis and associated activities in TNBC cells, projects it as a putative therapeutic factor for selective eradication of TNBC cells. (PMID:26917489)
• we identified KDM2B, a reader for methylated CpGs, as the target of miR-448 that represses glycolysis and promotes oxidative phosphorylation in gastric cancer (PMID:26989077)
• DCLK1 has been identified as a potential target for miR-448 to regulate lung squamous cell carcinoma cells growth. (PMID:28320089)
• The results suggest that miR-448 might promote Th17 differentiation in multiple sclerosis and thus aggravate the disease through inhibiting PTPN2. (PMID:28342869)
• we revealed that PVT1 functions as an endogenous “sponge” by competing for miR-448 binding to regulate the miRNA target SERBP1 and, therefore, promotes the proliferation and migration of PC cells. (PMID:28657147)
• Overexpression of miR-448 promoted VSMCs migration. (PMID:28799067)
• miR448 was downregulated in osteosarcoma and overexpression of miR448 inhibited cell proliferation and invasion by targeting AEG1, providing novel insights into the understanding of osteosarcoma pathogenesis. (PMID:28849015)
• LncRNA OIP5-AS1 modulated the expression of Bcl-2 by targeting miR-448 in lung adenocarcinoma cells. (PMID:29247949)
• we uncovered that NEAT1 participated in breast cancer progression by regulating miR-448 and ZEB1. NEAT1 can be provided as a vital biomarker in breast cancer diagnosis and treatment therapy. (PMID:29323713)
• miR-448 contributed to the progression of osteoarthritis by directly targeting matrilin-3. (PMID:29483929)
• Interference SNHG1 could inhibit the differentiation of Treg cells by promoting miR-448 expression and reducing IDO level, thereby impeding the immune escape of breast cancer . (PMID:29886172)
• Low miR448 expression is associated with pancreatic cancer. (PMID:30015954)
• Functional assays revealed the anti-oncogenic function of miR-448 in non-small cell lung cancer (NSCLC) by inhibiting cell proliferation, invasion, migration and epithelial-mesenchymal transition; lncRNA PRNCR1 interacted with HEY2 to abolish miR-448-mediated growth inhibition in NSCLC cells (PMID:30257372)
• miRNA-448 was expressed at a lower level in hepatocellular carcinoma (HCC) tissues than adjacent non-cancerous tissues, and correlated with a larger tumor size, incomplete tumor encapsulation, and advanced Barcelona Clinic Liver Cancer stage. miRNA-448 inhibited HCC cell growth. (PMID:30316787)
• microRNA-448 inhibits the progression of non-small-cell lung cancer through regulating IRS2. (PMID:30912183)
• miR-448 activates the AMPK signaling pathway by downregulating MAGEA6, thus inhibiting the stemness maintenance and self-renewal of hepatocellular carcinoma stem cells. (PMID:31232474)
• miR-448 targets IDO1 and regulates CD8(+) T cell response in human colon cancer. (PMID:31391111)
• MicroRNA-448 targets SATB1 to reverse the cisplatin resistance in lung cancer via mediating Wnt/beta-catenin signalling pathway. (PMID:32525527)
• MicroRNA-448/EPHA7 axis regulates cell proliferation, invasion and migration via regulation of PI3K/AKT signaling pathway and epithelial-to-mesenchymal transition in non-small cell lung cancer. (PMID:32572879)
• Long non-coding RNA NEAT1 regulates Hodgkin’s lymphoma cell proliferation and invasion via miR-448 mediated regulation of DCLK1. (PMID:32572888)
• Circular RNA circ-ZEB1 acts as an oncogene in triple negative breast cancer via sponging miR-448. (PMID:32629026)
• MiR-448 targets BLC2 and inhibits the growth of pituitary adenoma cells. (PMID:32648768)
• Inhibitory role of long non-coding RNA OIP5-AS1 in rheumatoid arthritis progression through the microRNA-448-paraoxonase 1-toll-like receptor 3-nuclear factor kappaB axis. (PMID:32770578)
• Tumor expression of miR-448 is a prognostic marker in oral squamous cell carcinoma. (PMID:32787801)
• MIR448 antagomir reduces arrhythmic risk after myocardial infarction by upregulating the cardiac sodium channel. (PMID:33108349)
• LncRNA XIST regulates cell proliferation, migration and invasion of glioblastoma via regulating miR-448 and ROCK1. (PMID:33325210)
• Long Noncoding RNA DUXAP8 Promotes Pancreatic Carcinoma Cell Migration and Invasion Via Pathway by miR-448/WTAP/Fak Signaling Axis. (PMID:33625109)
• Long noncoding RNA nuclear paraspeckle assembly transcript 1 regulates ionizing radiationinduced pyroptosis via microRNA448/gasdermin E in colorectal cancer cells. (PMID:34476497)
• Inhibition of microRNA-448 suppresses CD4(+) T cell inflammatory activation via up-regulating suppressor of cytokine signaling 5 in systemic lupus erythematosus. (PMID:35121374)
• Peripheral Blood Levels of miR-448 and SIRT1 in Patients with Deep Venous Thrombosis and Their Relationship. (PMID:35536066)
• Circulating miR-448 acts as a potential diagnostic biomarker for multiple myeloma. (PMID:35938956)
• miR-448 regulates potassium voltage-gated channel subfamily A member 4 (KCNA4) in ischemia and heart failure. (PMID:36693615)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir448	ENSMUSG00000065450
rattus_norvegicus	Mir448	ENSRNOG00000035635

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.