MIR449B

Summary

MIR449B (microRNA 449b, HGNC:32794) is a microRNA gene on chromosome 5q11.2.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 693123 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32794
Approved symbol	MIR449B
Name	microRNA 449b
Location	5q11.2
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-449b
Ensembl gene	ENSG00000207728
Ensembl biotype	miRNA
OMIM	613132
Entrez	693123
RNAcentral	URS000075B9B1 — miRNA, 97 nt, 9 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000384995

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000384995 — 1 exons

Exon	Start	End
ENSE00001500002	55170646	55170742

Expression profiles

Bgee: expression breadth broad, 12 present calls, max score 86.39.

Top tissues by expression

12 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
kidney	UBERON:0002113	86.39	gold quality
calcaneal tendon	UBERON:0003701	84.91	gold quality
intestine	UBERON:0000160	82.23	gold quality
lung	UBERON:0002048	69.08	gold quality
thyroid gland	UBERON:0002046	68.63	gold quality
stomach	UBERON:0000945	67.16	gold quality
right ovary	UBERON:0002118	64.47	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	64.09	gold quality
spleen	UBERON:0002106	64.05	gold quality
skin of abdomen	UBERON:0001416	63.68	gold quality
right frontal lobe	UBERON:0002810	50.60	gold quality
left testis	UBERON:0004533	41.90	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.50

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 22)

• Data suggest that miR-449a and b provide a twofold safety mechanism to avoid excessive E2F1-induced proliferation by cell cycle arrest and by apoptosis. (PMID:19960022)
• miR-449b and miR-34c expressionresulted in cell cycle arrests and down-regulation of CDK6, CDC25A and CyclinA in the malignant ovarian cancer cell line SKOV3-ipl. (PMID:21321636)
• miR-449b can regulate the expression level of E2F3 and CCND1 through the post-transcriptional regulation. (PMID:23674142)
• a role for miR-449b in regulation of HDAC1 and antiviral cytokine signaling (PMID:24086750)
• This study suggests that MIR449A and MIR449B could serve as viable therapeutic targets for retinoblastoma treatment. (PMID:24120948)
• This study provides the first evidence that miR-449b rs10061133 and miR-4293 rs12220909 are associated with esophageal squamous cell carcinoma risk in Chinese population. (PMID:26055141)
• NEAT1 functions a molecular sponge for miR-449b-5p and leads to the upregulation of c-Met. This regulation menchaism promotes glioma pathogenesis and may provide a potential target for the prognosis and treatment of glioma. (PMID:26242266)
• Rs10061133 A>G is a highly conserved single nucleotide polymorphism locus in the mature area of miR-449b. Association analysis shows that the rs10061133 AA genotype is a risk factor for premature ovarian insufficiency. (PMID:27552335)
• The study showed associations between miRNA polymorphisms (miR-27a rs895819 and miR-449b rs10061133) and recurrent pregnancy loss development, and between the miRNA polymorphism (miR-27a rs895819) and plasma folate levels. (PMID:28489914)
• Results suggest that the eukaryotic elongation factor-2 kinase (eEF-2 kinase) 3’ untranslated regions (3’UTR) contains a functional target site for miR-449b in cancer cells. (PMID:29331413)
• miR-449b-3p was downregulated in ectopic and eutopic tissues, and the same expression pattern was also observed in ESCs. Its expression is not affected by estrogen or progesterone. The upregulated expression of miR-449b-3p inhibited the proliferation of ESCs and the supernatants of miR-449b-overexpressing ESCs inhibited the formation of tubular structures in HUVECs. (PMID:30066926)
• miR-449b inhibits tumor cell growth and invasion by targeting LGR4 in NSCLC. (PMID:30128865)
• MiR-449b-5p overexpression decreased the expression of beta-catenin and suppressed the activation of Wnt/beta-catenin/TCF-4 signaling via targeting CREPT. (PMID:30738779)
• miR-449b-5p interacted with FOXP1 to regulate cell proliferation, migration, invasion and radiosensitivity in CC (PMID:31103631)
• study suggests that miR-449b-5p, as a novel tumour-suppressive miRNA against nasopharyngeal carcinoma, inhibits proliferation, migration and invasion of nasopharyngeal carcinoma cells via inhibiting TPD52 expression (PMID:31350893)
• C-Met is the downstream target of microRNA-449b-5p, and its level was inhibited in OS cells overexpressing microRNA-449b-5p. Importantly, c-Met partially rescued the inhibitory effects of microRNA-449b-5p on behavior of OS cells. MicroRNA-449b-5p is downregulated in OS, which alleviates the malignant progression of OS by targeting c-Met. (PMID:31425497)
• Feedback loop in miR-449b-3p/ADAM17/NF-kappaB promotes metastasis in nasopharyngeal carcinoma. (PMID:31433128)
• CASC19 in HeLa cells negatively regulates the expression of miR-449b-5p, and down-regulating the expression of miR-449b-5p could partially reverse the effects of silencing CASC19 on HeLa cell proliferation, apoptosis and radiation sensitivity. (PMID:32074771)
• MiR-449b-5p targets lncRNA PSMG3-AS1 to suppress cancer cell proliferation in lung adenocarcinoma. (PMID:32471413)
• MiR-449b-5p inhibits human glioblastoma cell proliferation by inactivating WNT2B/Wnt/beta-catenin signaling pathway. (PMID:32495889)
• MDM4 is targeted by miR-449b-5p to promote the proliferation of endometrial carcinoma. (PMID:33275219)
• CircSTX6 promotes pancreatic ductal adenocarcinoma progression by sponging miR-449b-5p and interacting with CUL2. (PMID:35650603)

Cross-species orthologs

0 orthologs

Paralogs (1): MIR449A (ENSG00000198983)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.