MIR4516

Summary

MIR4516 (microRNA 4516, HGNC:41617) is a microRNA gene on chromosome 16p13.3.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100616258 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:41617
Approved symbol	MIR4516
Name	microRNA 4516
Location	16p13.3
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-4516
Ensembl gene	ENSG00000265867
Ensembl biotype	miRNA
Entrez	100616258
RNAcentral	URS000075C976 — miRNA, 86 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000583451

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000583451 — 1 exons

Exon	Start	End
ENSE00002696722	2133119	2133204

Expression profiles

Bgee: expression breadth ubiquitous, 116 present calls, max score 97.24.

Top tissues by expression

116 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	97.24	gold quality
prefrontal cortex	UBERON:0000451	95.58	gold quality
right hemisphere of cerebellum	UBERON:0014890	89.02	gold quality
cerebellar hemisphere	UBERON:0002245	88.01	gold quality
endometrium	UBERON:0001295	86.62	gold quality
skeletal muscle tissue	UBERON:0001134	84.91	gold quality
bone marrow	UBERON:0002371	83.75	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	82.60	gold quality
adrenal tissue	UBERON:0018303	81.96	gold quality
right uterine tube	UBERON:0001302	81.71	gold quality
fundus of stomach	UBERON:0001160	81.40	gold quality
muscle of leg	UBERON:0001383	80.92	gold quality
calcaneal tendon	UBERON:0003701	80.88	gold quality
right testis	UBERON:0004534	80.76	gold quality
duodenum	UBERON:0002114	80.51	gold quality
blood	UBERON:0000178	80.38	gold quality
pituitary gland	UBERON:0000007	80.24	gold quality
urinary bladder	UBERON:0001255	80.24	gold quality
left uterine tube	UBERON:0001303	80.24	gold quality
gastrocnemius	UBERON:0001388	80.13	gold quality
vermiform appendix	UBERON:0001154	79.58	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	79.25	gold quality
monocyte	CL:0000576	78.88	gold quality
adenohypophysis	UBERON:0002196	78.68	gold quality
anterior cingulate cortex	UBERON:0009835	78.68	gold quality
left ovary	UBERON:0002119	78.55	gold quality
lower esophagus	UBERON:0013473	78.40	gold quality
lower esophagus muscularis layer	UBERON:0035833	78.40	gold quality
brain	UBERON:0000955	78.29	gold quality
myometrium	UBERON:0001296	78.23	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.32

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 18)

• overexpression of hsa-miR-4516 downregulates STAT3, p-STAT3, CDK6, and UBE2N proteins that are consistently upregulated in psoriasis and induces apoptosis in HaCaT cells. (PMID:24610393)
• Results provide evidence that loss of expression of miR-4516 in psoriatic skin might be contributing to accelerated migration, resistance to apoptosis and differentiation as seen in psoriasis lesional keratinocytes. (PMID:28844950)
• the data suggested that the opposite expression of miR-4516 in Enterovirus 71 and coxsackievirus A16 infections might be the initial steps leading to different epithelial impairments of 16HBE cells by destroying intercellular adhesion, which finally resulted in different outcomes of Enterovirus 71 and coxsackievirus A16 infections. (PMID:29686973)
• LSINCT5 could bind to HMGA2 and decrease proteasome-mediated HMGA2 degradation leading to EMT activation. LSINCT5 also served as a competing endogenous RNA (ceRNA) for miR-4516, resulting in increased STAT3/BclxL expression and attenuated apoptosis. (PMID:30472720)
• MiR-4516 represents an independent negative prognostic factor in GBM patients and acts as a novel oncogene in GBM, which regulates the PTPN14/Hippo pathway. (PMID:30559405)
• circRNA0047905 directly binds miR4516 and miR1227-5p, relieving suppression for targets SERPINB5 and MMP11. Down-regulated circRNA0047905 expression in gastric cancer cells inhibited Akt/CREB signaling pathway activation. (PMID:31157588)
• Down-regulation of miRNA 145 and up-regulation of miRNA 4516 may be associated with primary hypertension. (PMID:31556476)
• Our results show that the loss of miR-4516 from CAF-derived exosomes is associated with FOSL1-dependent triple negative breast cancer progression and suggest that miR-4516 can be used as an anti-cancer drug for triple negative breast cancer. (PMID:31672492)
• Melatonin-stimulated exosomes enhance the regenerative potential of chronic kidney disease-derived mesenchymal stem/stromal cells via cellular prion proteins. (PMID:31989677)
• MicroRNA-4516-mediated regulation of MAPK10 relies on 3’ UTR cis-acting variants and contributes to the altered risk of Hirschsprung disease. (PMID:32066630)
• Expression and clinical significance of miR-4516 and miR-21-5p in serum of patients with colorectal cancer. (PMID:32293319)
• MicroRNA-4516 suppresses pancreatic cancer development via negatively regulating orthodenticle homeobox 1. (PMID:32549762)
• miR-4516 inhibits the apoptosis of RB tumor cells by targeting the PTEN/AKT signaling pathway. (PMID:32919989)
• LINC00520 up-regulates SOX5 to promote cell proliferation and invasion by miR-4516 in human hepatocellular carcinoma. (PMID:35089659)
• Streptococcus gordonii-Induced miRNAs Regulate CCL20 Responses in Human Oral Epithelial Cells. (PMID:35099275)
• MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency. (PMID:36139370)
• Extracellular vesicles from cerebrospinal fluid revealed changes in miR-19a-3p and miR-4516 expression in Slovene male suicides. (PMID:37794714)
• Temporin-GHaK Exhibits Antineoplastic Activity against Human Lung Adenocarcinoma by Inhibiting the Wnt Signaling Pathway through miRNA-4516. (PMID:38930863)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.