MIR466

Summary

MIR466 (microRNA 466, HGNC:38359) is a microRNA gene on chromosome 3p23.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 100423038 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:38359
Approved symbol	MIR466
Name	microRNA 466
Location	3p23
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-466
Ensembl gene	ENSG00000265376
Ensembl biotype	miRNA
Entrez	100423038
RNAcentral	URS00007B235C — miRNA, 84 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000580653

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000580653 — 1 exons

Exon	Start	End
ENSE00002687784	31161704	31161787

Expression profiles

Bgee: expression breadth broad, 14 present calls, max score 82.39.

Top tissues by expression

14 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	82.39	gold quality
Ammon’s horn	UBERON:0001954	78.23	gold quality
monocyte	CL:0000576	74.22	gold quality
minor salivary gland	UBERON:0001830	68.04	gold quality
lower esophagus muscularis layer	UBERON:0035833	66.98	gold quality
subcutaneous adipose tissue	UBERON:0002190	66.93	gold quality
right adrenal gland	UBERON:0001233	66.31	gold quality
left ovary	UBERON:0002119	65.36	gold quality
ectocervix	UBERON:0012249	65.04	gold quality
tibial nerve	UBERON:0001323	64.53	gold quality
thyroid gland	UBERON:0002046	62.54	gold quality
skin of leg	UBERON:0001511	61.29	gold quality
lung	UBERON:0002048	58.91	gold quality
vagina	UBERON:0000996	57.84	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.98

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 12)

• Subsequent experiments also proved that both the rno-miR-466d and the human hsa-miR-466, which are orthologs of the miR-467 gene family, could effectively down-regulate the levels of rat and human CAR protein expression, respectively (PMID:25497012)
• In primary lymphatic endothelial cells (HDLEC), miR-466 mimic transfection suppressed Prox1 mRNA and protein expression. HDLEC transfected with the miR-466 mimic suppressed tube formation as compared to the scrambled control. (PMID:25573115)
• Our findings implicate miR-466g as a novel early-phase aldosterone responsive miRNA that regulates SGK1 and ENaC in cortical collecting duct cells. (PMID:26911843)
• miR-466-mediated attenuation of RUNX2 as a novel therapeutic approach to regulate prostate cancer growth, particularly metastasis to bone. (PMID:28125091)
• MicroRNA-466 (miR-466) has a role as a tumor suppressor and prognostic factor in colorectal cancer (PMID:29338680)
• the expression level of miR-466 was significantly downregulated in tumor tissues and cell lines, and its overexpression was able to inhibit cell proliferation and invasion. The tumor-suppressive roles of miR-466 in OS cells were mediated by the silencing of IRS1. (PMID:30816452)
• miR-466 was downregulated in tumor tissues of prostate carcinoma patients. TUC338 and miR-466 were inversely correlated in tumor tissues. miR-466 overexpression failed to affect TUC338 expression but resulted in reduced rates of cancer cell migration and invasion, and also attenuated the effect of TUC338 overexpression. (PMID:31085276)
• NUS1 was upregulated in IUAs tissues, and the high expression level of NUS1 was positively correlated with the severity of IUAs. NUS1 promoted cell proliferation in vitro. NUS1 overexpression on cell migration and invasion promoted the EMT process in vitro and in vivo. (PMID:31154456)
• PGM5-AS1 was transcriptionally activated by p53 and it could directly interact with and sequester miR-466 to elevate PTEN expression, thereby inhibiting esophageal squamous cell carcinoma (ESCC) progression. Overall, our data indicate that PGM5-AS1 is a novel tumor suppressor in ESCC and restoration of PGM5-AS1 may be a promising avenue for treatment of ESCC patient. (PMID:31185143)
• MicroRNA-466 regulates the proliferation, migration and invasion of the human lung cancer cells by targeting transcription factor RUNX2. (PMID:33455109)
• The Clinical Significance and Functional Role of miR-466 in Gastric Cancer Peritoneal Metastasis. (PMID:34435325)
• A novel ceRNA regulatory network involving the long noncoding NEAT1, miRNA-466f-3p and its mRNA target in osteoblast autophagy and osteoporosis. (PMID:36169673)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.