MIR496

Summary

MIR496 (microRNA 496, HGNC:32086) is a microRNA gene on chromosome 14q32.31.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574454 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32086
Approved symbol	MIR496
Name	microRNA 496
Location	14q32.31
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-496
Ensembl gene	ENSG00000207961
Ensembl biotype	miRNA
OMIM	621334
Entrez	574454
RNAcentral	URS000075BE7B — miRNA, 102 nt, 9 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385226

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385226 — 1 exons

Exon	Start	End
ENSE00001500232	101060573	101060674

Expression profiles

Bgee: expression breadth broad, 55 present calls, max score 90.56.

Top tissues by expression

55 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
olfactory segment of nasal mucosa	UBERON:0005386	90.56	gold quality
adrenal tissue	UBERON:0018303	84.65	gold quality
blood	UBERON:0000178	82.67	gold quality
saliva-secreting gland	UBERON:0001044	80.90	gold quality
placenta	UBERON:0001987	78.52	gold quality
muscle of leg	UBERON:0001383	77.68	gold quality
vagina	UBERON:0000996	76.20	gold quality
intestine	UBERON:0000160	75.98	gold quality
left ovary	UBERON:0002119	75.50	gold quality
stomach	UBERON:0000945	75.14	gold quality
colon	UBERON:0001155	74.95	gold quality
monocyte	CL:0000576	74.31	gold quality
omental fat pad	UBERON:0010414	73.83	gold quality
gastrocnemius	UBERON:0001388	73.68	gold quality
right atrium auricular region	UBERON:0006631	73.21	gold quality
adipose tissue	UBERON:0001013	73.20	gold quality
muscle layer of sigmoid colon	UBERON:0035805	72.73	gold quality
islet of Langerhans	UBERON:0000006	72.51	gold quality
body of stomach	UBERON:0001161	72.30	gold quality
body of pancreas	UBERON:0001150	71.64	gold quality
putamen	UBERON:0001874	71.64	gold quality
heart	UBERON:0000948	71.37	gold quality
heart left ventricle	UBERON:0002084	71.22	gold quality
endometrium	UBERON:0001295	69.81	gold quality
left adrenal gland	UBERON:0001234	69.65	gold quality
esophagus mucosa	UBERON:0002469	69.59	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	69.57	gold quality
multicellular organism	UBERON:0000468	69.40	gold quality
ascending aorta	UBERON:0001496	69.32	gold quality
left adrenal gland cortex	UBERON:0035825	69.31	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.16

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): MBD2

Literature-anchored findings (GeneRIF, showing 19)

• Methylated DNA binding domain protein 2 (MBD2) coordinately silences gene expression through activation of the microRNA hsa-mir-496 promoter in breast cancer cell line. (PMID:24204564)
• miR-496 is involved in the regulation of human aging through the control of mTOR. (PMID:27097372)
• Long non-coding RNA DANCR contributes to lung adenocarcinoma progression by sponging miR-496 to modulate mTOR expression. (PMID:29266795)
• Over-expression of the high risk Human papillomavirus 16 E6 oncoprotein down-regulated miR-496, impacting upon the post-transcriptional control of the transcription factor E2F2. (PMID:29935424)
• BCL2L14 knockdown exhibited similar effects similar to those of miR-496 overexpression, and the restored BCL2L14expression reversed the protective effects of miR-496 on SH-SY5Y cells (PMID:30597231)
• Findings reveal that, through epigenetic modification, ox-LDL can inhibit the normal expression of Hippo-YAP/ZAP pathway proteins via miR-496 expression and induce vascular endothelial cell dysfunction. (PMID:30811087)
• miR-496 could work as a tumor suppressor in osteosarcoma cells through down-regulation of eIF4E. (PMID:30998968)
• MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer. (PMID:31273789)
• findings first represent a mechanism of miR-496 on NSCLC tumor growth via inactivating BDNF-mediated PI3K/Akt signaling pathway (PMID:31421833)
• miR-496 remedies hypoxia reoxygenation-induced H9c2 cardiomyocyte apoptosis via Hook3-targeted PI3k/Akt/mTOR signaling pathway activation. (PMID:31436348)
• Long noncoding RNA NNT-AS1 enhances the malignant phenotype of bladder cancer by acting as a competing endogenous RNA on microRNA-496 thereby increasing HMGB1 expression. (PMID:31848324)
• Hsa_circ_001193 regulates proliferation and apoptosis of nasopharyngeal carcinoma cells through targeting mir-496. (PMID:32271424)
• miR-496/MMP10 Is Involved in the Proliferation of IL-1beta-Induced Fibroblast-Like Synoviocytes Via Mediating the NF-kappaB Signaling Pathway. (PMID:33548006)
• MicroRNA-496 inhibits triple negative breast cancer cell proliferation by targeting Del-1. (PMID:33832090)
• The effect of miR-496 on eIF3h in lung invasive adenocarcinoma. (PMID:34038061)
• The long noncoding RNA noncoding RNA activated by DNA damage (NORAD)-microRNA-496-Interleukin-33 axis affects carcinoma-associated fibroblasts-mediated gastric cancer development. (PMID:34895039)
• Knockdown of circ_0007290 alleviates oxygen-glucose deprivation-induced neuronal injury by regulating miR-496/PDCD4 axis. (PMID:35032277)
• Ras-related Protein in Brain 4A (Rab4A) is Downregulated by miR-496 to inhibit the Progression of Gastric Cancer. (PMID:38523522)
• Circ_0027791 contributes to the growth and immune evasion of hepatocellular carcinoma via the miR-496/programmed cell death ligand 1 axis in an m6A-dependent manner. (PMID:38546290)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mir496a	ENSMUSG00000070136
rattus_norvegicus	Mir496	ENSRNOG00000040406

Paralogs (16): MIR494 (ENSG00000194717), MIR377 (ENSG00000199015), MIR381 (ENSG00000199020), MIR369 (ENSG00000199025), MIR323A (ENSG00000199069), MIR410 (ENSG00000199092), MIR409 (ENSG00000199107), MIR539 (ENSG00000202560), MIR487A (ENSG00000207742), MIR487B (ENSG00000207754), MIR656 (ENSG00000207959), MIR154 (ENSG00000207978), MIR323B (ENSG00000208004), MIR1185-1 (ENSG00000221525), MIR1185-2 (ENSG00000221614), MIR382 (ENSG00000283170)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.