MIR500A

Summary

MIR500A (microRNA 500a, HGNC:32134) is a microRNA gene on chromosome Xp11.23.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Source: NCBI Gene 574502 — RefSeq curated summary.

At a glance

• Gene type: non-coding (miRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32134
Approved symbol	MIR500A
Name	microRNA 500a
Location	Xp11.23
Locus type	RNA, micro
Status	Approved
Aliases	hsa-mir-500
Ensembl gene	ENSG00000207785
Ensembl biotype	miRNA
Entrez	574502
RNAcentral	URS000075E243 — miRNA, 84 nt, 19 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 miRNA

ENST00000385051

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000385051 — 1 exons

Exon	Start	End
ENSE00001807648	50008431	50008514

Expression profiles

Bgee: expression breadth broad, 24 present calls, max score 79.63.

Top tissues by expression

24 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
blood	UBERON:0000178	79.63	gold quality
intestine	UBERON:0000160	74.96	gold quality
lung	UBERON:0002048	73.83	gold quality
adrenal tissue	UBERON:0018303	73.46	gold quality
heart	UBERON:0000948	73.06	gold quality
stomach	UBERON:0000945	72.34	gold quality
gastrocnemius	UBERON:0001388	72.33	gold quality
left adrenal gland cortex	UBERON:0035825	71.22	gold quality
colon	UBERON:0001155	69.69	gold quality
ascending aorta	UBERON:0001496	68.49	gold quality
lower esophagus muscularis layer	UBERON:0035833	66.60	gold quality
right adrenal gland	UBERON:0001233	65.05	gold quality
body of stomach	UBERON:0001161	64.37	gold quality
omental fat pad	UBERON:0010414	64.24	gold quality
caudate nucleus	UBERON:0001873	63.79	gold quality
ectocervix	UBERON:0012249	63.62	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	62.81	gold quality
thoracic mammary gland	UBERON:0005200	62.57	gold quality
tibial artery	UBERON:0007610	62.40	gold quality
subcutaneous adipose tissue	UBERON:0002190	62.36	gold quality
tibial nerve	UBERON:0001323	60.20	gold quality
spleen	UBERON:0002106	58.20	gold quality
skin of leg	UBERON:0001511	56.65	gold quality
right frontal lobe	UBERON:0002810	54.88	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.33

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 20)

• Fndings reveal that diverse changes of miRNAs occur during liver development and, one of these, miR-500 is an oncofetal miRNA relevant to the diagnosis of human HCC. (PMID:19863192)
• Data show that the constitutive nuclear factor-kappaB (NF-kappaB) activation indicating the potentially pivotal role of microRNA miR-500 in the progression of gastric cancer. (PMID:25595906)
• miR-192-5p, induced by LY6K, downregulates ERalpha directly and induced tamoxifen resistance in ERalpha-positive breast cancer cells; re-expression of ERalpha in ERalpha-negative breast cancer cells increased miR-500a-3p expression and directly inhibits LY6K expression (PMID:27304060)
• miR-500a promotes hepatocellular carcinoma migration and invasion through activating Wnt/beta-catenin signaling by directly binding to SFPR2 and GSK-3beta. (PMID:28437633)
• microRNA-500 associated with human Prostate cancer and may be a potential biomarker and molecular target in Prostate cancer. (PMID:28631332)
• Results uncover a novel mechanism by which miR-500a-3p promotes the stemness maintenance of cancer stem cell in HCC. (PMID:28750679)
• miR-500a-5p is identified as an oxidative stress response miRNA whose activity may define breast cancer progression and survival. (PMID:29162888)
• results suggest that histone modifications in the promoter region of miR-500a may be responsible for the increased expression of miR-500a in Hepatocellular carcinoma (HCC), which promotes cancer progression by targeting BID, indicating that miR-500a may be a potential prognostic predictor and therapeutic target for HCC patients. (PMID:29969781)
• MiR500a5p promoted cell proliferation, migration and invasion by targeting CHD5 and subsequently activating Wnt/betacatenin signaling. (PMID:30015879)
• miR-500a-3p acts as a tumor suppressor in non-small cell lung cancer partially via down-regulation of LY6K expression (PMID:30249107)
• Studies demonstrate that miR-500a-5p functions as a tumour suppressor in CRC by targeting the p300/YY1/HDAC2 axis, which contributes to the development of and provides new potential candidates for CRC therapy. (PMID:30737378)
• the findings demonstrate that miR-500 plays an important role in the proliferation of prostate cancer cells via the inhibition of LRP1B expression. (PMID:30877185)
• Mir-5100 Mediates Proliferation, Migration and Invasion of Oral Squamous Cell Carcinoma Cells Via Targeting SCAI. (PMID:31851859)
• MicroRNA500a5p inhibits colorectal cancer cell invasion and epithelialmesenchymal transition. (PMID:32236592)
• Exosomal MiR-500a-3p promotes cisplatin resistance and stemness via negatively regulating FBXW7 in gastric cancer. (PMID:32588541)
• Circ_0000524/miR-500a-5p/CXCL16 axis promotes podocyte apoptosis in membranous nephropathy. (PMID:32974919)
• Exosomal miR-500a-5p derived from cancer-associated fibroblasts promotes breast cancer cell proliferation and metastasis through targeting USP28. (PMID:33664871)
• LINC01278 Sponges miR-500b-5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection. (PMID:33910387)
• lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR500a3p/XBP1 axis. (PMID:33982777)
• Cancer-associated fibroblast exosomes promote prostate cancer metastasis through miR-500a-3p/FBXW7/HSF1 axis under hypoxic microenvironment. (PMID:38351137)

Cross-species orthologs

0 orthologs

Paralogs (3): MIR501 (ENSG00000211538), MIR500B (ENSG00000239057), MIR502 (ENSG00000272080)

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.